FastQCFastQC Report
Fri 27 May 2016
SRR940049_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940049_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775126
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14100.046.992621
GGTATCA8700.046.656851
CAACGCA15050.042.3492775
ATCAACG14850.042.150083
TCAACGC14900.042.0113564
AACGCAG15550.040.9875646
GTACATG33550.036.830161
TACATGG33500.035.4458472
TATCAAC17900.035.335392
CGTAGAC700.002762776634.0641483
ACATGGG36250.032.560633
ACGCAGA19950.032.2462737
CGCAGAG21350.029.852778
TATTCCG1055.865708E-428.364785
CATGGGG19250.027.253084
TGGACCG1107.6988805E-427.075475
CATGGGA20950.025.6107434
GCAGAGT24600.024.9289769
TCTATAC2752.068191E-923.8449043
ATGGGAG11000.022.7433955
Produced by FastQC (version 0.11.5)