FastQCFastQC Report
Fri 27 May 2016
SRR940049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940049_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775126
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26470.34149286696614484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18360.2368647161880778No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12100.1561036528254761No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13300.068.608831
GTATCAA25950.058.8359571
ATCAACG31400.047.8549543
TCAACGC32000.046.9576724
CAACGCA32600.046.1986735
TATCAAC32600.046.0934222
AACGCAG34350.043.82246
ACGCAGA39250.038.1975257
CGCAGAG40050.037.434538
GCAGAGT44100.033.8617559
GTACATG34300.031.8197821
TACATGG35450.030.4451642
ACATGGG34150.030.3818723
GTGGTAT6150.030.0626221
GCGTACA1055.829688E-428.3946632
TGGTATC6550.028.2212582
CATGGGG18900.027.1326754
CAGAGTA44000.026.29912410-11
AGAGTAC43000.026.21893910-11
GAGTACT28650.025.95731412-13
Produced by FastQC (version 0.11.5)