##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940049_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 775126 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.1568764304126 34.0 33.0 34.0 31.0 34.0 2 33.19958819598362 34.0 33.0 34.0 31.0 34.0 3 33.26981290783692 34.0 34.0 34.0 31.0 34.0 4 36.48756847273862 37.0 37.0 37.0 35.0 37.0 5 36.495328501430734 37.0 37.0 37.0 35.0 37.0 6 36.569030067369695 37.0 37.0 37.0 35.0 37.0 7 36.55951548522434 37.0 37.0 37.0 35.0 37.0 8 36.55534584054721 37.0 37.0 37.0 35.0 37.0 9 38.44256288655006 39.0 39.0 39.0 37.0 39.0 10-11 38.41727409479233 39.0 39.0 39.0 37.0 39.0 12-13 38.39888869680542 39.0 39.0 39.0 37.0 39.0 14-15 40.02534426660956 41.0 40.0 41.0 38.0 41.0 16-17 40.0304840761373 41.0 40.0 41.0 38.0 41.0 18-19 40.06242017426844 41.0 40.0 41.0 38.0 41.0 20-21 40.0623601840217 41.0 40.0 41.0 38.0 41.0 22-23 40.02751875179003 41.0 40.0 41.0 38.0 41.0 24-25 40.00537977051473 41.0 40.0 41.0 38.0 41.0 26-27 39.927834958445466 41.0 40.0 41.0 38.0 41.0 28-29 39.8561575795419 41.0 40.0 41.0 38.0 41.0 30-31 39.74291599043252 41.0 40.0 41.0 37.0 41.0 32-33 39.6102717493672 41.0 40.0 41.0 36.0 41.0 34-35 39.519669705312424 41.0 40.0 41.0 36.0 41.0 36-37 39.438544830130844 41.0 40.0 41.0 35.5 41.0 38-39 39.33759737126609 41.0 40.0 41.0 35.0 41.0 40-41 39.22796809808986 41.0 40.0 41.0 35.0 41.0 42-43 39.09104777803867 41.0 39.0 41.0 35.0 41.0 44-45 38.919114440748984 41.0 39.0 41.0 35.0 41.0 46-47 38.69003039505835 41.0 39.0 41.0 35.0 41.0 48-49 38.46595650255571 41.0 39.0 41.0 35.0 41.0 50-51 38.492636035947704 41.0 39.0 41.0 35.0 41.0 52-53 38.40061293260709 41.0 39.0 41.0 35.0 41.0 54-55 38.253209800729174 41.0 38.5 41.0 35.0 41.0 56-57 38.084086974246766 41.0 38.0 41.0 35.0 41.0 58-59 37.875677631765676 40.5 37.0 41.0 34.0 41.0 60-61 37.651528267662286 40.0 37.0 41.0 34.0 41.0 62-63 37.39645425388905 39.5 36.0 41.0 34.0 41.0 64-65 37.103530264756955 39.0 36.0 41.0 34.0 41.0 66-67 36.78170387782116 39.0 35.0 41.0 33.5 41.0 68-69 36.42804718200654 37.5 35.0 41.0 33.0 41.0 70-71 36.06852498819546 37.0 35.0 40.0 33.0 41.0 72-73 35.69522309921226 37.0 35.0 39.0 33.0 41.0 74-75 35.33021470057771 36.0 35.0 39.0 32.0 41.0 76-77 34.95546917533407 36.0 35.0 38.0 32.0 40.5 78-79 34.615185144092706 35.0 35.0 37.0 32.0 39.0 80-81 34.29932875429285 35.0 35.0 37.0 32.0 39.0 82-83 34.01623477989385 35.0 35.0 36.0 31.5 38.0 84-85 33.766552147650835 35.0 35.0 36.0 31.0 37.0 86-87 33.560561121675704 35.0 35.0 36.0 31.0 37.0 88-89 33.45396812905257 35.0 35.0 35.0 31.5 36.5 90-91 33.44950175842379 35.0 35.0 35.0 32.0 36.0 92-93 33.361979858758446 35.0 35.0 35.0 32.0 36.0 94-95 33.29298075409675 35.0 35.0 35.0 32.0 36.0 96-97 33.22646834192118 35.0 35.0 35.0 32.0 36.0 98-99 33.179312137639556 35.0 35.0 35.0 32.0 36.0 100-101 33.12613626687791 35.0 35.0 35.0 31.0 35.0 102-103 32.65913670809649 35.0 33.5 35.0 30.0 35.0 104-105 33.04125716335151 35.0 35.0 35.0 31.0 35.0 106-107 33.08605310620467 35.0 35.0 35.0 32.0 35.0 108-109 33.07536194115537 35.0 35.0 35.0 32.0 35.0 110-111 33.04513395241548 35.0 35.0 35.0 31.5 35.0 112-113 33.01942522893052 35.0 35.0 35.0 31.5 35.0 114-115 32.96581200991839 35.0 35.0 35.0 31.0 35.0 116-117 32.918588074713014 35.0 35.0 35.0 31.0 35.0 118-119 32.869300604030826 35.0 35.0 35.0 31.0 35.0 120-121 32.80057177800771 35.0 35.0 35.0 31.0 35.0 122-123 32.710685101518976 35.0 35.0 35.0 31.0 35.0 124-125 31.66534602116301 34.5 33.0 35.0 27.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1385510690756604 1101 2 0.11006863554822388 1101 3 0.138508889785534 1101 4 -0.07987685685088763 1101 5 0.06107476790096911 1101 6 0.0979704501109282 1101 7 0.09052035198051556 1101 8 0.12520728056518493 1101 9 0.18674312841879015 1101 10-11 0.22428455729485108 1101 12-13 0.19605805403843846 1101 14-15 0.2236711315505744 1101 16-17 0.2125549921354306 1101 18-19 0.20461225495338908 1101 20-21 0.2195154537287607 1101 22-23 0.1909141815291946 1101 24-25 0.15280364813852287 1101 26-27 0.3018222677920548 1101 28-29 0.33567564387914217 1101 30-31 0.2980803111477641 1101 32-33 0.3329355100876725 1101 34-35 0.3485115563096741 1101 36-37 0.37618214585148024 1101 38-39 0.36350270515792005 1101 40-41 0.31336469168650183 1101 42-43 0.33052657014734876 1101 44-45 0.25455799457184014 1101 46-47 0.38675250572548237 1101 48-49 0.3925013534296511 1101 50-51 0.2693312064453792 1101 52-53 0.2916382919168541 1101 54-55 0.2641223344938908 1101 56-57 0.25639276200783456 1101 58-59 0.2515053742689659 1101 60-61 0.2474386327763085 1101 62-63 0.29375939032270537 1101 64-65 0.208196826940501 1101 66-67 0.21112760191186197 1101 68-69 0.17866311244515742 1101 70-71 0.3080767097460466 1101 72-73 0.3066099420296382 1101 74-75 0.2266303772145264 1101 76-77 0.25019552666352496 1101 78-79 0.26396790761184263 1101 80-81 0.2613153123200078 1101 82-83 0.25307367173784456 1101 84-85 0.30786822167107175 1101 86-87 0.2708890565094606 1101 88-89 0.2398759814104281 1101 90-91 0.3203388237839704 1101 92-93 0.24193564287018177 1101 94-95 0.3250223031032675 1101 96-97 0.28287535708534506 1101 98-99 0.409144213305936 1101 100-101 0.4048912815189212 1101 102-103 0.4176103585120714 1101 104-105 0.3527565791514746 1101 106-107 0.29540279484154297 1101 108-109 0.3456854713425699 1101 110-111 0.3579352017648887 1101 112-113 0.3491187523552597 1101 114-115 0.36030767653194573 1101 116-117 0.4721136989657637 1101 118-119 0.40989028782999526 1101 120-121 0.4489959736746343 1101 122-123 0.5661997528014169 1101 124-125 0.5443422013509682 1102 1 0.12713834992239015 1102 2 0.1431620825243911 1102 3 0.11631459514681808 1102 4 0.07540999739152454 1102 5 0.10127052226739863 1102 6 0.07346565940881788 1102 7 0.14520357110161797 1102 8 0.07972389550543824 1102 9 0.17361001589330272 1102 10-11 0.13889658695971008 1102 12-13 0.16793555670609805 1102 14-15 0.22049766576211027 1102 16-17 0.18560881793089834 1102 18-19 0.1931335374728178 1102 20-21 0.20704565212306392 1102 22-23 0.20700945427421402 1102 24-25 0.1837995684436038 1102 26-27 0.11297554142000621 1102 28-29 0.21793351875518852 1102 30-31 0.2156855590685396 1102 32-33 0.16474293251468453 1102 34-35 0.18403485094617622 1102 36-37 0.10024203018247846 1102 38-39 0.15339786210083162 1102 40-41 0.08412865282845416 1102 42-43 0.025904357883412388 1102 44-45 0.014124424384007739 1102 46-47 0.26081105899778123 1102 48-49 0.30512566876632263 1102 50-51 0.41390026509848354 1102 52-53 0.3051063875489817 1102 54-55 0.333838752437444 1102 56-57 0.43465979013088685 1102 58-59 0.44893742926831237 1102 60-61 0.3548961109968616 1102 62-63 0.3927774017653718 1102 64-65 0.3145240144800212 1102 66-67 0.27647204662745395 1102 68-69 0.28502302184443096 1102 70-71 0.3049487217965634 1102 72-73 0.3487218663190248 1102 74-75 0.2817844583514315 1102 76-77 0.20449806299588147 1102 78-79 0.29570034706531345 1102 80-81 0.20346639191954097 1102 82-83 0.2591377533406174 1102 84-85 0.2849069044553758 1102 86-87 0.3354770161961298 1102 88-89 0.35212305252667875 1102 90-91 0.3359127652040854 1102 92-93 0.3402820242761777 1102 94-95 0.31407711831904095 1102 96-97 0.31557447792107496 1102 98-99 0.30916728499009594 1102 100-101 0.3647526517551185 1102 102-103 0.3921228028017154 1102 104-105 0.3803422161407042 1102 106-107 0.2773824784488923 1102 108-109 0.2281047370862197 1102 110-111 0.31837669175200034 1102 112-113 0.3309974973443772 1102 114-115 0.2568078795183979 1102 116-117 0.33962122499350045 1102 118-119 0.375858999076776 1102 120-121 0.40253647890014577 1102 122-123 0.37405311006421726 1102 124-125 0.36074206469560366 1103 1 0.12144960641003877 1103 2 0.1149643527031401 1103 3 0.027996131883206488 1103 4 -0.12433648010835441 1103 5 0.012253985686143665 1103 6 0.08257385412473184 1103 7 0.10343884175461682 1103 8 0.03955697841881545 1103 9 0.08752538724614567 1103 10-11 0.12871358094519536 1103 12-13 0.07118591903939375 1103 14-15 0.14370738471558298 1103 16-17 0.09485374637284849 1103 18-19 0.1130966614728024 1103 20-21 0.13585214619941155 1103 22-23 0.10732236840897968 1103 24-25 0.13478595712196295 1103 26-27 0.059406724687526946 1103 28-29 0.15671248879787925 1103 30-31 0.18321739342793109 1103 32-33 0.21240693542220868 1103 34-35 0.19031972691072951 1103 36-37 0.20862289898459352 1103 38-39 0.1399715852914909 1103 40-41 0.19953679302817307 1103 42-43 0.20581447263337083 1103 44-45 0.2700491831608929 1103 46-47 0.18274540908043946 1103 48-49 0.1232077621884855 1103 50-51 0.17622513733999057 1103 52-53 0.19441744458710986 1103 54-55 0.19592051787594755 1103 56-57 0.10452983768851709 1103 58-59 0.04848490047001519 1103 60-61 0.12170416521166771 1103 62-63 0.03197876156787771 1103 64-65 0.16797737210187336 1103 66-67 0.25982244397521725 1103 68-69 0.17954414354851878 1103 70-71 0.23581841034505402 1103 72-73 0.1802490734495663 1103 74-75 0.16625664844011823 1103 76-77 0.14196399036966767 1103 78-79 0.16707978426308756 1103 80-81 0.2889770491721819 1103 82-83 0.18303224897710635 1103 84-85 0.22913527858722205 1103 86-87 0.21442002238792668 1103 88-89 0.16153130796389803 1103 90-91 0.274406965655281 1103 92-93 0.2061411420001491 1103 94-95 0.2247458947184029 1103 96-97 0.13658404706579574 1103 98-99 0.08772734810479221 1103 100-101 0.16162550490265204 1103 102-103 0.08728858967439379 1103 104-105 0.0965277662446411 1103 106-107 0.2037656608654217 1103 108-109 0.17066770773168116 1103 110-111 0.14154142375832635 1103 112-113 0.13642673487102996 1103 114-115 0.12302268221850454 1103 116-117 0.0439672300196321 1103 118-119 0.10556168632663088 1103 120-121 0.12636205364717767 1103 122-123 0.10464999599239633 1103 124-125 0.17981475739708586 1104 1 0.1258920034703408 1104 2 0.17936204854289883 1104 3 0.14677427008589916 1104 4 -0.1271143112034281 1104 5 -0.040909755993695285 1104 6 0.01490780807138492 1104 7 0.11669245506773507 1104 8 0.13811506547956043 1104 9 0.17289597444018767 1104 10-11 0.16605328249630702 1104 12-13 0.18045225172652124 1104 14-15 0.13205115757536134 1104 16-17 0.2897577859044276 1104 18-19 0.26394461974028616 1104 20-21 0.2419530310532494 1104 22-23 0.24544348824279894 1104 24-25 0.26199355974175376 1104 26-27 0.32579699449436816 1104 28-29 0.28336079298797756 1104 30-31 0.2676869183281738 1104 32-33 0.25661017242185835 1104 34-35 0.3383893209101956 1104 36-37 0.38427406311441814 1104 38-39 0.39863937801943905 1104 40-41 0.29123401050516406 1104 42-43 0.3083900179093533 1104 44-45 0.28086299703235085 1104 46-47 0.29043195030217817 1104 48-49 0.319070446692983 1104 50-51 0.33729082211534234 1104 52-53 0.28252267586687907 1104 54-55 0.2606269208565948 1104 56-57 0.2514925646335797 1104 58-59 0.2749581106617285 1104 60-61 0.21995087926674728 1104 62-63 0.3113976457008647 1104 64-65 0.35155308586050893 1104 66-67 0.43406389881226204 1104 68-69 0.3757283313448312 1104 70-71 0.3965481961588182 1104 72-73 0.400253829352323 1104 74-75 0.3767869209341512 1104 76-77 0.4199955584739783 1104 78-79 0.36118246484343786 1104 80-81 0.3398289529195395 1104 82-83 0.36183713113522487 1104 84-85 0.28514509750035444 1104 86-87 0.3256766897157277 1104 88-89 0.39478487862317024 1104 90-91 0.26024749149178206 1104 92-93 0.3477966653520568 1104 94-95 0.3195179206263461 1104 96-97 0.2809859443084406 1104 98-99 0.27948939941308737 1104 100-101 0.33391821258950927 1104 102-103 0.3898290521699366 1104 104-105 0.2894110802771124 1104 106-107 0.2789686398093565 1104 108-109 0.2520858838109845 1104 110-111 0.2809362937814228 1104 112-113 0.37582725652745097 1104 114-115 0.4049704056766217 1104 116-117 0.3642276956715662 1104 118-119 0.37643700703016947 1104 120-121 0.4217500009526347 1104 122-123 0.40185179769044055 1104 124-125 0.3431464272307956 1105 1 0.03647934412629894 1105 2 0.06885317907405408 1105 3 0.051286161099938 1105 4 0.04242187958672616 1105 5 -3.496405357594767E-5 1105 6 0.03387235244299802 1105 7 -0.0017413975641886736 1105 8 0.00825839674325124 1105 9 0.033018049719942155 1105 10-11 0.03863872609110075 1105 12-13 0.1381207878141737 1105 14-15 0.11768011020748759 1105 16-17 0.1527037115711778 1105 18-19 0.1045014820253769 1105 20-21 0.18652921585694315 1105 22-23 0.10933440520289395 1105 24-25 0.18659043424246136 1105 26-27 0.19146969279085368 1105 28-29 0.25591913570886504 1105 30-31 0.27601386407991413 1105 32-33 0.3278005277466747 1105 34-35 0.29881446081340357 1105 36-37 0.23589323920740668 1105 38-39 0.2743570961258541 1105 40-41 0.18129092234035227 1105 42-43 0.21701456766069072 1105 44-45 0.2130723856649439 1105 46-47 0.28137793259762134 1105 48-49 0.32935765528274885 1105 50-51 0.27566414272070006 1105 52-53 0.22535550594641762 1105 54-55 0.23587158714169476 1105 56-57 0.26385481028480484 1105 58-59 0.24094752514128004 1105 60-61 0.29278043297198053 1105 62-63 0.3274053633825673 1105 64-65 0.3050396941861351 1105 66-67 0.2634224373066303 1105 68-69 0.3039580375430475 1105 70-71 0.4238578883616526 1105 72-73 0.3075460880079177 1105 74-75 0.2951030667853942 1105 76-77 0.29303554844261015 1105 78-79 0.31281751719463813 1105 80-81 0.2882862255603982 1105 82-83 0.2655643102780658 1105 84-85 0.21652123538313361 1105 86-87 0.3427704648067973 1105 88-89 0.22221854721184542 1105 90-91 0.26923881314476716 1105 92-93 0.27688439735273107 1105 94-95 0.32534460851528735 1105 96-97 0.3104288191044233 1105 98-99 0.37188821101953806 1105 100-101 0.3472330337338576 1105 102-103 0.37712400068839 1105 104-105 0.3334312926751579 1105 106-107 0.2846107788368428 1105 108-109 0.25448451339664757 1105 110-111 0.25773420418270376 1105 112-113 0.2500792016218796 1105 114-115 0.29857419494507553 1105 116-117 0.25348371600935593 1105 118-119 0.28964898642541215 1105 120-121 0.1477869633087039 1105 122-123 0.3222844408953023 1105 124-125 0.42830182242827775 1106 1 0.11484479498550115 1106 2 0.04394913797412414 1106 3 0.12517943511918617 1106 4 0.03732572384789279 1106 5 0.031593089407834896 1106 6 0.07496441393543307 1106 7 0.08109623013496048 1106 8 0.053432894312294366 1106 9 0.006285376452616731 1106 10-11 0.10749391180847567 1106 12-13 0.1447739382186768 1106 14-15 0.1610289281906745 1106 16-17 0.1225962376031049 1106 18-19 0.13883508437169212 1106 20-21 0.12699729845048324 1106 22-23 0.07798783118167307 1106 24-25 0.1366986955656131 1106 26-27 0.12600800745730822 1106 28-29 0.11571678545395514 1106 30-31 0.07430235098028959 1106 32-33 0.024417853495151576 1106 34-35 0.09786274536352124 1106 36-37 0.12565073384354264 1106 38-39 0.2469925704088709 1106 40-41 0.22204442114947653 1106 42-43 0.1893439576573499 1106 44-45 0.13513210654757302 1106 46-47 0.1605169598204199 1106 48-49 0.1631479826256168 1106 50-51 0.1565373195016022 1106 52-53 0.09344498885097607 1106 54-55 0.199589280978671 1106 56-57 0.1623312782099191 1106 58-59 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2216 124-125 -0.5991615952150191 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 14.0 5 21.0 6 64.0 7 150.0 8 247.0 9 456.0 10 844.0 11 1320.0 12 1720.0 13 2579.0 14 4533.0 15 4722.0 16 2126.0 17 1672.0 18 1573.0 19 1359.0 20 1226.0 21 1098.0 22 1162.0 23 1277.0 24 1588.0 25 2063.0 26 2555.0 27 3119.0 28 3968.0 29 4976.0 30 6272.0 31 7863.0 32 10205.0 33 13689.0 34 20261.0 35 35529.0 36 91783.0 37 313287.0 38 227477.0 39 2323.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.38865964216866 18.406275080580436 13.752222574909483 23.452842702341414 2 15.356510228857545 19.425604848853904 41.44103231666174 23.776852605626807 3 19.755806812845428 24.27276151944721 28.557108282029713 27.414323385677648 4 12.585190018152337 16.14356496405062 37.68748263900104 33.583762378796 5 13.774398711469265 37.42430656293719 33.904206825702474 14.897087899891073 6 32.070556540453495 36.272544055904355 17.492303603178165 14.164595800463978 7 28.916847067444518 32.110908420050414 20.491378175935267 18.480866336569797 8 25.963520769526504 35.84320484669589 19.388073680924133 18.805200702853472 9 26.609867298993972 16.5000270923695 19.784396343304188 37.10570926533235 10-11 25.171778523749687 26.72191359856333 27.03695915244747 21.069348725239507 12-13 26.07659915936248 23.648219773301374 27.78264437007661 22.492536697259542 14-15 22.84712420948336 24.88853425120561 26.120592006976928 26.1437495323341 16-17 22.4113240944053 28.35668007523938 26.453699140526833 22.778296689828494 18-19 22.193037003016283 26.79996542497607 29.074627866953247 21.932369705054406 20-21 23.13036848202744 25.910368120795845 29.19189912349734 21.767364273679377 22-23 22.688956376124654 25.916044617262223 28.967742018716958 22.42725698789616 24-25 22.466540923669186 25.95906988025173 29.16196850576551 22.412420690313574 26-27 22.303148133335743 26.370551368422685 29.01612124996452 22.310179248277056 28-29 22.22551201419126 26.310595065312047 29.151685212062574 22.312207708434123 30-31 22.446458601166654 26.013691993995803 29.22698224607789 22.31286715875966 32-33 22.391148272118517 26.172664942644776 28.9968527677131 22.439334017523613 34-35 22.602154754793755 26.231062682954835 28.792398109719358 22.374384452532052 36-37 22.601289220565345 26.09507308227957 28.71944261497743 22.58419508217765 38-39 22.485911713403997 26.188473407713982 28.843444560269997 22.48217031861202 40-41 22.755104015481372 26.114949201741656 28.63363973552653 22.496307047250443 42-43 22.470743202011562 26.049935558282016 28.663211713298214 22.816109526408205 44-45 22.748726814148732 26.272750436221372 28.45995310418676 22.51856964544314 46-47 22.716192410132294 26.482421233722796 28.054682757824377 22.74670359832053 48-49 22.86468093341289 26.438549242851845 27.95265203438211 22.744117789353158 50-51 22.817849021916174 26.78204776725959 27.73313551258688 22.666967698237354 52-53 22.926788676937683 26.684500326398545 27.67446840900705 22.714242587656717 54-55 22.739593304830443 26.648119144500377 27.723879730521233 22.88840782014795 56-57 22.784624772776237 26.853623388469096 27.61260558083489 22.749146257919772 58-59 22.968125395505574 26.560364229589013 27.678068623321302 22.79344175158412 60-61 22.87421487912509 26.6757577380004 27.830611924999953 22.61941545787456 62-63 22.670056223117275 26.710173571780587 27.919525341686384 22.700244863415755 64-65 22.59592900431225 26.534557663838065 27.935506552232404 22.934006779617285 66-67 22.649229445372253 26.854494799860923 27.610503358497063 22.885772396269754 68-69 22.680365155420695 26.62335735535825 27.905220081483943 22.791057407737107 70-71 22.648592929361996 26.46644846417527 27.837266697715584 23.04769190874715 72-73 22.704953775257184 26.578324040220558 27.643441195366947 23.07328098915531 74-75 22.67995484599258 26.849153362360912 27.461312691501373 23.00957910014514 76-77 22.883574333250763 26.606494096276112 27.49295578399802 23.016975786475104 78-79 22.752978407873357 26.748034660417314 27.449796515509522 23.049190416199806 80-81 22.692697703341132 26.823058444691572 27.475726527042056 23.008517324925236 82-83 22.82577284209277 26.8284769185913 27.422380361386406 22.923369877929524 84-85 22.934811636346748 26.655898483208475 27.342370178668418 23.066919701776364 86-87 22.75221593434345 26.591921684839015 27.504484763415427 23.151377617402108 88-89 22.726627670856093 26.76055247791972 27.49436865748278 23.018451193741406 90-91 22.748008385744235 26.789614578293826 27.50517658442187 22.957200451540075 92-93 22.651689220134664 26.55862762071986 27.556149297980575 23.2335338611649 94-95 22.639470789912497 26.758007656903633 27.49737942957036 23.10514212361352 96-97 22.70042651350368 26.644773338906653 27.485608717079373 23.169191430510296 98-99 22.633868558144098 26.49911111225788 27.33710390310737 23.52991642649066 100-101 22.521672324897484 26.519739732133353 27.54938077689455 23.409207166074612 102-103 22.633675041219107 26.371712469972625 27.514816945890086 23.479795542918183 104-105 22.576394031421987 26.37635687617239 27.49243348823288 23.55481560417274 106-107 22.495855507176262 26.46631188517981 27.439896790840184 23.59793581680374 108-109 22.51504275444961 26.37261909062292 27.387230946274403 23.725107208653068 110-111 22.38023237512353 26.501110787149447 27.48108049530012 23.637576342426907 112-113 22.515673288613634 26.47426316675579 27.477198824188886 23.532864720441683 114-115 22.336843354654928 26.458366124208826 27.40229179191706 23.802498729219185 116-117 22.27684451846873 26.32840550056056 27.652843878568632 23.741906102402076 118-119 22.17163543193973 26.547636479512025 27.342281991754884 23.93844609679336 120-121 22.192148239220614 26.54163745096762 27.526445717499943 23.739768592311826 122-123 22.189159411913447 26.52665896037279 27.354268478333786 23.92991314937997 124-125 22.155508623814864 26.430841120289607 27.36327618526656 24.050374070628973 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 68.0 1 52.0 2 98.0 3 160.5 4 97.5 5 59.5 6 181.0 7 499.5 8 895.0 9 994.0 10 722.5 11 463.5 12 416.5 13 474.0 14 523.0 15 569.5 16 644.5 17 732.5 18 810.5 19 884.5 20 974.0 21 1089.0 22 1333.5 23 1683.5 24 2029.0 25 2590.0 26 3440.0 27 4410.0 28 5428.0 29 6779.0 30 8275.0 31 9530.0 32 10945.0 33 12433.0 34 14159.5 35 15731.5 36 17698.5 37 19556.5 38 20364.0 39 21300.5 40 22450.0 41 23668.0 42 24869.5 43 26078.5 44 27643.0 45 29138.0 46 29389.0 47 29538.0 48 30084.0 49 29913.0 50 29569.0 51 28988.5 52 28514.0 53 28482.0 54 28163.0 55 26428.5 56 23479.0 57 20920.0 58 19336.5 59 17373.0 60 14548.5 61 11820.5 62 9231.0 63 6998.5 64 5266.5 65 3841.0 66 2742.5 67 2191.0 68 1626.5 69 1016.5 70 750.5 71 597.5 72 428.5 73 320.0 74 246.0 75 178.5 76 110.5 77 57.5 78 34.0 79 14.5 80 7.5 81 4.0 82 2.5 83 1.5 84 0.5 85 1.5 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01599739913252813 2 0.0019351692499025965 3 2.5802256665367955E-4 4 0.1446216486093874 5 0.03741327216478353 6 0.0113529929327619 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.2901128332683977E-4 30-31 1.9351692499025965E-4 32-33 6.450564166341989E-5 34-35 3.870338499805193E-4 36-37 8.385733416244584E-4 38-39 0.0018061579665757568 40-41 1.2901128332683977E-4 42-43 9.030789832878784E-4 44-45 4.515394916439392E-4 46-47 6.450564166341989E-5 48-49 0.0017416523249123368 50-51 0.0017416523249123368 52-53 0.0 54-55 0.0 56-57 0.0011611015499415579 58-59 0.0013546184749318175 60-61 8.385733416244584E-4 62-63 0.0 64-65 0.001096595908278138 66-67 3.225282083170994E-4 68-69 5.160451333073591E-4 70-71 6.450564166341988E-4 72-73 0.0 74-75 1.2901128332683977E-4 76-77 0.002838248233190475 78-79 7.095620582976187E-4 80-81 0.0 82-83 0.0 84-85 3.870338499805193E-4 86-87 1.9351692499025965E-4 88-89 0.0 90-91 1.2901128332683977E-4 92-93 6.450564166341988E-4 94-95 0.001096595908278138 96-97 5.160451333073591E-4 98-99 0.0 100-101 1.9351692499025965E-4 102-103 0.0 104-105 0.0 106-107 1.2901128332683977E-4 108-109 2.5802256665367955E-4 110-111 0.0 112-113 1.9351692499025965E-4 114-115 0.0015481353999220772 116-117 6.450564166341988E-4 118-119 6.450564166341989E-5 120-121 6.450564166341989E-5 122-123 2.5802256665367955E-4 124-125 6.450564166341988E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 775126.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.3961483367469 #Duplication Level Percentage of deduplicated Percentage of total 1 81.11856594653393 54.67078903396788 2 11.716686676846413 15.793191065758542 3 3.3682599236189366 6.810232363468251 4 1.3534070259975148 3.648576827364959 5 0.6777887391281093 2.284017520162736 6 0.4074241211309666 1.6475289902226822 7 0.28347287956833683 1.3373486168582696 8 0.19903871737536064 1.073155433678852 9 0.13071205179123238 0.7928539948730199 >10 0.6996309644736047 8.29266515516132 >50 0.030839330050487463 1.3986128784192775 >100 0.013407481683282508 1.4430693554647092 >500 1.9153545046438544E-4 0.07306353423283742 >1k 5.746063513931563E-4 0.7348952303666845 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2647 0.34149286696614484 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1836 0.2368647161880778 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1210 0.1561036528254761 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.2901128332683977E-4 0.0 12-13 0.0 0.0 0.0 1.2901128332683977E-4 0.0 14-15 0.0 0.0 0.0 1.2901128332683977E-4 0.0 16-17 0.0 0.0 0.0 1.2901128332683977E-4 0.0 18-19 0.0 0.0 0.0 1.2901128332683977E-4 0.0 20-21 0.0 0.0 0.0 1.2901128332683977E-4 0.0 22-23 0.0 0.0 0.0 3.2252820831709945E-4 0.0 24-25 0.0 0.0 0.0 8.385733416244585E-4 0.0 26-27 0.0 0.0 0.0 9.675846249512982E-4 0.0 28-29 0.0 0.0 0.0 0.0014836297582586572 0.0 30-31 0.0 0.0 0.0 0.0035478102914880934 0.0 32-33 0.0 0.0 0.0 0.008772767266225103 0.0 34-35 0.0 0.0 0.0 0.014578275015932892 0.0 36-37 0.0 0.0 0.0 0.022125435090553022 0.0 38-39 0.0 0.0 0.0 0.03509106906490042 0.0 40-41 0.0 0.0 0.0 0.052378581030696945 0.0 42-43 0.0 0.0 0.0 0.07011763248813742 0.0 44-45 0.0 0.0 0.0 0.09146899987872939 0.0 46-47 0.0 0.0 0.0 0.11488454780255081 0.0 48-49 0.0 0.0 0.0 0.14184790601786032 0.0 50-51 0.0 0.0 0.0 0.1739717155662434 0.0 52-53 0.0 0.0 0.0 0.20596651383129969 0.0 54-55 0.0 0.0 0.0 0.2400254926295854 0.0 56-57 0.0 0.0 0.0 0.2849214192273256 0.0 58-59 0.0 0.0 0.0 0.33510680844146634 0.0 60-61 0.0 0.0 0.0 0.38858198538044136 0.0 62-63 0.0 0.0 0.0 0.4439278259276557 0.0 64-65 0.0 0.0 0.0 0.5004992736664748 0.0 66-67 0.0 0.0 0.0 0.5662950281631631 0.0 68-69 0.0 0.0 0.0 0.6444113602175646 0.0 70-71 0.0 0.0 0.0 0.7359448657379575 0.0 72-73 0.0 0.0 0.0 0.8307036533415213 0.0 74-75 0.0 0.0 0.0 0.9229467209202117 0.0 76-77 0.0 0.0 0.0 1.020092217265322 0.0 78-79 0.0 0.0 0.0 1.1362023722594778 0.0 80-81 0.0 0.0 0.0 1.2683099263861617 0.0 82-83 0.0 0.0 0.0 1.4066745277541974 0.0 84-85 0.0 0.0 0.0 1.5383305423892373 0.0 86-87 0.0 0.0 0.0 1.6858549448734785 0.0 88-89 0.0 0.0 0.0 1.8506668593235163 0.0 90-91 0.0 0.0 0.0 2.041732569930566 0.0 92-93 0.0 0.0 0.0 2.2487311740284808 0.0 94-95 0.0 0.0 0.0 2.4556652724847314 0.0 96-97 0.0 0.0 0.0 2.6629864047909626 0.0 98-99 0.0 0.0 0.0 2.892690994754401 0.0 100-101 0.0 0.0 0.0 3.159808856882623 0.0 102-103 0.0 0.0 0.0 3.4533740320928468 0.0 104-105 0.0 0.0 0.0 3.7490678934779638 0.0 106-107 0.0 0.0 0.0 4.041471967138246 0.0 108-109 0.0 0.0 0.0 4.3444549660313285 0.0 110-111 0.0 0.0 0.0 4.695946207455304 0.0 112-113 0.0 0.0 0.0 5.069627389611496 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1330 0.0 68.60883 1 GTATCAA 2595 0.0 58.835957 1 ATCAACG 3140 0.0 47.854954 3 TCAACGC 3200 0.0 46.957672 4 CAACGCA 3260 0.0 46.198673 5 TATCAAC 3260 0.0 46.093422 2 AACGCAG 3435 0.0 43.8224 6 ACGCAGA 3925 0.0 38.197525 7 CGCAGAG 4005 0.0 37.43453 8 GCAGAGT 4410 0.0 33.861755 9 GTACATG 3430 0.0 31.819782 1 TACATGG 3545 0.0 30.445164 2 ACATGGG 3415 0.0 30.381872 3 GTGGTAT 615 0.0 30.062622 1 GCGTACA 105 5.829688E-4 28.394663 2 TGGTATC 655 0.0 28.221258 2 CATGGGG 1890 0.0 27.132675 4 CAGAGTA 4400 0.0 26.299124 10-11 AGAGTAC 4300 0.0 26.218939 10-11 GAGTACT 2865 0.0 25.957314 12-13 >>END_MODULE