FastQCFastQC Report
Fri 27 May 2016
SRR940048_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940048_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425997
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28170.6612722624807217No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5080.11924966607746064No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4740.11126838921400856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9400.055.2157941
GTATCAA16750.050.932281
TCAACGC19800.040.332164
ATCAACG20000.039.919453
CAACGCA20300.039.324885
AACGCAG20800.038.379576
TATCAAC22350.037.894872
GTACATG34750.034.5074731
TACATGG36100.032.878772
CGCAGAG24500.031.853998
ACGCAGA25100.031.5672367
CATTCCG800.00533507129.7766279
ACATGGG37250.029.1108463
GCAGAGT26100.028.7498469
TACAAGG2701.8189894E-1228.7176722
CATGGGA28750.026.9474324
GTATGGT1358.0865495E-526.5148641
GAACAAA7300.026.1516461
CATGGGG8550.025.7897724
GGATATG1701.3675941E-524.565241
Produced by FastQC (version 0.11.5)