##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940048_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 425997 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.78208531984967 34.0 31.0 34.0 26.0 34.0 2 30.78731305619523 34.0 31.0 34.0 26.0 34.0 3 30.999446005488302 34.0 31.0 34.0 26.0 34.0 4 33.88313063237535 37.0 35.0 37.0 27.0 37.0 5 34.263600447890475 37.0 35.0 37.0 31.0 37.0 6 34.05941591137966 37.0 36.0 37.0 30.0 37.0 7 34.09550771484306 37.0 36.0 37.0 30.0 37.0 8 33.934969025603465 37.0 36.0 37.0 28.0 37.0 9 35.359864036601195 39.0 38.0 39.0 27.0 39.0 10-11 35.49760326950659 39.0 38.0 39.0 27.5 39.0 12-13 35.42823541010853 39.0 38.0 39.0 27.0 39.0 14-15 36.85460930476036 41.0 39.0 41.0 27.0 41.0 16-17 36.95398911259939 41.0 39.0 41.0 28.0 41.0 18-19 37.00941790669887 41.0 39.0 41.0 28.5 41.0 20-21 37.07401812688822 41.0 39.0 41.0 28.0 41.0 22-23 37.16688028319449 41.0 39.0 41.0 29.0 41.0 24-25 37.31640011549377 41.0 39.0 41.0 30.0 41.0 26-27 37.41416254105076 41.0 39.0 41.0 30.0 41.0 28-29 37.38994171320455 41.0 39.0 41.0 30.0 41.0 30-31 37.33696950917495 41.0 38.0 41.0 30.0 41.0 32-33 37.2947555968704 41.0 38.0 41.0 30.0 41.0 34-35 37.20607539489715 41.0 38.0 41.0 30.0 41.0 36-37 37.05394052070789 41.0 38.0 41.0 30.0 41.0 38-39 36.952228536820684 41.0 38.0 41.0 29.5 41.0 40-41 36.86279598213133 41.0 38.0 41.0 29.0 41.0 42-43 36.68931706091827 40.5 38.0 41.0 28.5 41.0 44-45 36.53784181578743 40.0 37.0 41.0 28.0 41.0 46-47 36.39954506721878 40.0 37.0 41.0 28.0 41.0 48-49 36.26411218858348 40.0 36.5 41.0 28.0 41.0 50-51 36.120996157249934 40.0 36.0 41.0 28.0 41.0 52-53 36.266549999178395 40.0 36.0 41.0 29.0 41.0 54-55 36.085513513005964 40.0 36.0 41.0 28.5 41.0 56-57 35.99953520799443 40.0 35.5 41.0 29.0 41.0 58-59 35.81581208318369 40.0 35.0 41.0 28.5 41.0 60-61 35.58691141017425 39.5 35.0 41.0 28.0 41.0 62-63 35.315546823099695 39.0 35.0 41.0 28.0 41.0 64-65 35.02839339244173 39.0 35.0 41.0 27.5 41.0 66-67 34.72322809785045 38.0 35.0 41.0 27.0 41.0 68-69 34.38146160653714 37.0 35.0 40.0 26.0 41.0 70-71 34.0293793148778 37.0 34.5 39.5 26.0 41.0 72-73 33.66350349885093 36.0 34.0 39.0 26.0 41.0 74-75 33.29380723338427 36.0 34.0 39.0 25.5 41.0 76-77 32.926362157479986 35.0 34.0 37.5 25.0 40.0 78-79 32.56169644387167 35.0 34.0 37.0 25.0 39.0 80-81 32.227502775841145 35.0 34.0 37.0 24.0 39.0 82-83 31.887709303117155 35.0 33.0 36.0 23.0 38.0 84-85 31.603724908860862 35.0 33.0 36.0 21.5 37.0 86-87 31.3673781740247 35.0 33.0 35.5 20.0 37.0 88-89 31.16388730437069 35.0 33.0 35.0 19.5 36.5 90-91 30.978185292384687 35.0 33.0 35.0 18.0 36.0 92-93 30.80582140249814 35.0 33.0 35.0 16.5 36.0 94-95 30.670113874041366 35.0 33.0 35.0 13.0 36.0 96-97 30.546953382300813 35.0 32.0 35.0 9.0 35.5 98-99 30.464551393554416 35.0 32.0 35.0 7.0 35.0 100-101 30.356260724840784 35.0 32.0 35.0 7.0 35.0 102-103 29.672284077117915 34.0 31.0 35.0 6.0 35.0 104-105 30.498975344896795 35.0 33.0 35.0 3.5 35.0 106-107 30.5809219313751 35.0 33.0 35.0 2.0 35.0 108-109 30.545689288891708 35.0 33.0 35.0 2.0 35.0 110-111 30.481450573595588 35.0 33.0 35.0 2.0 35.0 112-113 30.402230532139896 35.0 33.0 35.0 2.0 35.0 114-115 30.316023352277128 35.0 33.0 35.0 2.0 35.0 116-117 30.215441658039843 35.0 33.0 35.0 2.0 35.0 118-119 30.11881304328434 35.0 33.0 35.0 2.0 35.0 120-121 30.00104343457818 35.0 33.0 35.0 2.0 35.0 122-123 29.84955410484111 35.0 32.0 35.0 2.0 35.0 124-125 28.65155153674791 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24855573522368246 1101 2 0.23364526791849372 1101 3 0.29636593512351794 1101 4 0.15857517461379445 1101 5 0.4744652067385928 1101 6 0.42024436888119965 1101 7 0.42286845404067464 1101 8 0.17173328697948165 1101 9 0.5068615955791458 1101 10-11 0.505419534290418 1101 12-13 0.24516685573152586 1101 14-15 0.46009873164850035 1101 16-17 0.3158909525208813 1101 18-19 0.3187171270867637 1101 20-21 0.11741900675300343 1101 22-23 0.17412475203473576 1101 24-25 -0.01939372171389664 1101 26-27 0.24773147317176125 1101 28-29 0.3514237194336829 1101 30-31 0.35712713000379637 1101 32-33 0.4569829797879734 1101 34-35 0.47819226825141214 1101 36-37 0.5137871386492492 1101 38-39 0.45419399383393255 1101 40-41 0.36189166264797024 1101 42-43 0.37885024316396 1101 44-45 0.38307552382415366 1101 46-47 0.41800324198790406 1101 48-49 0.41638394106941234 1101 50-51 0.5312558607748414 1101 52-53 0.29007868484787025 1101 54-55 0.32424729137603236 1101 56-57 0.32390834898637877 1101 58-59 0.3967656417259491 1101 60-61 0.41997598715477835 1101 62-63 0.3247206436704815 1101 64-65 0.44307195154406287 1101 66-67 0.38260879816741067 1101 68-69 0.310572953418486 1101 70-71 0.37677921795302893 1101 72-73 0.4392408087705775 1101 74-75 0.26604181218673517 1101 76-77 0.32440242695458466 1101 78-79 0.41750181225431504 1101 80-81 0.33190764136532636 1101 82-83 0.23228378413533335 1101 84-85 0.21941637066280606 1101 86-87 0.13497323994752364 1101 88-89 0.2511443492254699 1101 90-91 0.22795769399589716 1101 92-93 0.20348959228421393 1101 94-95 0.26350751819260054 1101 96-97 0.4314030667897484 1101 98-99 0.4296932846260546 1101 100-101 0.29343427663293653 1101 102-103 0.33547092393092726 1101 104-105 0.3494822363850112 1101 106-107 0.3541605858388266 1101 108-109 0.5285632590217801 1101 110-111 0.5197140004215477 1101 112-113 0.4948027020826089 1101 114-115 0.6720729863212682 1101 116-117 0.608971252748276 1101 118-119 0.6298960157774829 1101 120-121 0.6763869726014669 1101 122-123 0.7189405201139287 1101 124-125 0.5960577170982582 1102 1 -0.13291462016461963 1102 2 -0.17774262986444 1102 3 -0.016889820822214574 1102 4 -0.18192304139548554 1102 5 -0.26587467331649606 1102 6 -0.05937258824879876 1102 7 0.14243306631641417 1102 8 -0.10466778739142057 1102 9 0.04526529442271254 1102 10-11 0.030874388661104035 1102 12-13 -0.0875663915653746 1102 14-15 0.10072672530060345 1102 16-17 0.05341838115221975 1102 18-19 0.0030791018759259714 1102 20-21 0.015962894370623815 1102 22-23 0.13598494644815418 1102 24-25 0.07176874615799989 1102 26-27 0.19089862605271435 1102 28-29 0.20542567875073559 1102 30-31 0.3567584024392474 1102 32-33 0.32553883297655517 1102 34-35 0.33711059509586505 1102 36-37 0.3234116510793541 1102 38-39 0.45223829175134966 1102 40-41 0.3223528613017592 1102 42-43 0.3201104961399821 1102 44-45 0.17537526318437102 1102 46-47 0.37684673882660036 1102 48-49 0.30463418417656385 1102 50-51 0.32792791375592145 1102 52-53 0.33645340604283547 1102 54-55 0.4911724288354904 1102 56-57 0.3781311833445642 1102 58-59 0.13667172464627697 1102 60-61 0.005721413306240208 1102 62-63 0.06191007203430843 1102 64-65 0.2164328309954513 1102 66-67 0.18308019105386109 1102 68-69 0.14451541022854997 1102 70-71 0.14935203260959184 1102 72-73 0.1311725435614619 1102 74-75 0.27060752703018665 1102 76-77 0.2316963642781289 1102 78-79 0.29291166017226544 1102 80-81 0.32620501653115497 1102 82-83 0.3083248068843858 1102 84-85 0.31255623047403347 1102 86-87 0.06367868084808848 1102 88-89 0.17872914752787494 1102 90-91 0.21617829429883528 1102 92-93 0.3209275141067387 1102 94-95 0.16426919366173465 1102 96-97 0.1676218740645261 1102 98-99 0.24455408997043548 1102 100-101 0.336416342736392 1102 102-103 0.4097568757722172 1102 104-105 0.34615284337565555 1102 106-107 0.23719984086454815 1102 108-109 0.272697056518048 1102 110-111 0.19143440370828202 1102 112-113 0.3598646808482364 1102 114-115 0.3501304427517695 1102 116-117 0.2493190857508374 1102 118-119 0.2272147879869948 1102 120-121 0.4885491982699648 1102 122-123 0.5422278348012135 1102 124-125 0.6971270270354673 1103 1 -0.23418103667493995 1103 2 -0.3298533240483046 1103 3 -0.163794136019213 1103 4 -0.32418382938798374 1103 5 -0.5135294575566505 1103 6 -0.33643637811746885 1103 7 -0.6166194665166103 1103 8 -0.7803713521193814 1103 9 -0.9467140676785988 1103 10-11 -0.8743788946409623 1103 12-13 -0.6090016636103996 1103 14-15 -0.5636025429920863 1103 16-17 -0.4586829321686068 1103 18-19 -0.7289799975612254 1103 20-21 -0.608896392683782 1103 22-23 -0.6313461980171411 1103 24-25 -0.42384570037108915 1103 26-27 -0.22861356906923191 1103 28-29 -0.21544674151192567 1103 30-31 -0.3058119540335511 1103 32-33 -0.14357467546621905 1103 34-35 -0.2010929696320929 1103 36-37 -0.3017994183390371 1103 38-39 -0.23028985083777087 1103 40-41 -0.2924454501428997 1103 42-43 -0.27229100479809887 1103 44-45 -0.31969978371994756 1103 46-47 -0.5701748371583903 1103 48-49 -0.6570449903074973 1103 50-51 -0.6123394408406995 1103 52-53 -0.5148364626250839 1103 54-55 -0.3910039313896476 1103 56-57 -0.3690311055860178 1103 58-59 -0.5526340914888124 1103 60-61 -0.5021116073316634 1103 62-63 -0.40530381164298745 1103 64-65 -0.4001009401114928 1103 66-67 -0.4233457001281309 1103 68-69 -0.3224639518727628 1103 70-71 -0.46150631635851624 1103 72-73 -0.2657317716355294 1103 74-75 -0.2388436924820141 1103 76-77 -0.19433553065621112 1103 78-79 -0.15741667003410953 1103 80-81 -0.15085877333751085 1103 82-83 -0.057341234391415696 1103 84-85 -0.16964358190870854 1103 86-87 -0.16791137543708956 1103 88-89 -0.28231681870101966 1103 90-91 -0.21377011095444942 1103 92-93 -0.22534359283509886 1103 94-95 -0.39114825474352344 1103 96-97 -0.30892596833697183 1103 98-99 -0.5248642965211197 1103 100-101 -0.5443528880328401 1103 102-103 -0.5183622611883827 1103 104-105 -0.41271207219658024 1103 106-107 -0.4187570071654747 1103 108-109 -0.4167494725626284 1103 110-111 -0.4850899865356233 1103 112-113 -0.41555745798853394 1103 114-115 -0.5557560488054563 1103 116-117 -0.3060280061816165 1103 118-119 -0.29264918949893826 1103 120-121 -0.22005774356868457 1103 122-123 -0.1503847355552601 1103 124-125 -0.3418035545780391 1104 1 0.4800131025192016 1104 2 0.3572345880396064 1104 3 0.5076344354093578 1104 4 0.45905792885377394 1104 5 0.48647054244334953 1104 6 0.7667687500876568 1104 7 0.6473915224943809 1104 8 0.7366616149135865 1104 9 0.9918390458745137 1104 10-11 0.9580386877766216 1104 12-13 0.9447529151441785 1104 14-15 0.9165714496819106 1104 16-17 0.8616467381610633 1104 18-19 0.8257361196548914 1104 20-21 0.7475138637264749 1104 22-23 0.6708058166348749 1104 24-25 0.8066030175776362 1104 26-27 0.6893351488794863 1104 28-29 0.7500294489642698 1104 30-31 0.8723931741715774 1104 32-33 0.7787238593323167 1104 34-35 0.920198239159113 1104 36-37 0.9710485670089497 1104 38-39 0.8953053872574728 1104 40-41 0.8657505205530782 1104 42-43 0.9337529512458573 1104 44-45 0.9847873957672348 1104 46-47 1.0626548331712797 1104 48-49 0.7983579400954355 1104 50-51 0.8204902294889749 1104 52-53 0.8083778230892023 1104 54-55 0.910094969709256 1104 56-57 0.9569304328755166 1104 58-59 0.953409864555141 1104 60-61 0.9637308835108271 1104 62-63 0.9610881297489513 1104 64-65 0.989505286994735 1104 66-67 0.9300425782601494 1104 68-69 0.8749719455631322 1104 70-71 0.9673856250334225 1104 72-73 1.070531964628202 1104 74-75 1.0103779192396019 1104 76-77 1.0925692312485538 1104 78-79 1.1952390076215664 1104 80-81 1.1001485526698147 1104 82-83 1.1126221355719537 1104 84-85 1.2369956122304906 1104 86-87 1.099258954233239 1104 88-89 1.0033516794674782 1104 90-91 1.1210742113898746 1104 92-93 0.9584018284103202 1104 94-95 1.0705275694323042 1104 96-97 0.9584732990622911 1104 98-99 0.9755020038451789 1104 100-101 0.9673687236887716 1104 102-103 0.9859875556614348 1104 104-105 0.9626542281697184 1104 106-107 0.9919572785488135 1104 108-109 1.0382871574740022 1104 110-111 1.0655968266511913 1104 112-113 1.1020249595938836 1104 114-115 0.8694728889234824 1104 116-117 0.8926899425363324 1104 118-119 0.8184771841274383 1104 120-121 0.8099330989221549 1104 122-123 0.6221885245180019 1104 124-125 0.6050828923084062 1105 1 -0.18986619850764086 1105 2 -0.04245315591043308 1105 3 0.029522383526817464 1105 4 -0.07647120503601457 1105 5 -0.35744146540308463 1105 6 -0.3287896077657777 1105 7 -0.0871708186253386 1105 8 -0.08479197703180574 1105 9 0.22675167422976727 1105 10-11 -0.058069520954539655 1105 12-13 -0.007162655623851322 1105 14-15 -0.08587555686345638 1105 16-17 -0.11462380368325142 1105 18-19 -0.12404269897638898 1105 20-21 -0.1631278624890271 1105 22-23 -0.1443576173810186 1105 24-25 -0.28797453601630707 1105 26-27 -0.34240087465981617 1105 28-29 -0.2700320014250508 1105 30-31 -0.23094156031070412 1105 32-33 -0.20913618590477512 1105 34-35 -0.24739919073339678 1105 36-37 -0.05023888870474025 1105 38-39 -0.10308829556244348 1105 40-41 -0.09012830007984718 1105 42-43 -0.18363330548466905 1105 44-45 -0.2276618125836123 1105 46-47 -0.12858698511971056 1105 48-49 -0.19744361718153414 1105 50-51 -0.2466798499761893 1105 52-53 -0.15650838360028274 1105 54-55 -0.21021368338627866 1105 56-57 -0.18766813795256354 1105 58-59 -0.2728806937026249 1105 60-61 -0.26502669770577114 1105 62-63 -0.22606533609303625 1105 64-65 -0.22176795706538854 1105 66-67 -0.32939516068578456 1105 68-69 -0.4615139728899891 1105 70-71 -0.5508243071109362 1105 72-73 -0.5199071954830856 1105 74-75 -0.3545663350489079 1105 76-77 -0.3756196836616752 1105 78-79 -0.47635670085938386 1105 80-81 -0.5089959458189455 1105 82-83 -0.35451091352830844 1105 84-85 -0.5079702597845674 1105 86-87 -0.49981728828562666 1105 88-89 -0.4719780909442548 1105 90-91 -0.3612112626988804 1105 92-93 -0.2833914420714727 1105 94-95 -0.3815381244366698 1105 96-97 -0.37302853243954104 1105 98-99 -0.5185731377709466 1105 100-101 -0.2912283258918862 1105 102-103 -0.36361411771073193 1105 104-105 -0.3604106850591293 1105 106-107 -0.3662664649208338 1105 108-109 -0.31274568946048475 1105 110-111 -0.13045393497334246 1105 112-113 -0.11105976988429234 1105 114-115 -0.16332400452914797 1105 116-117 -0.14874903742724044 1105 118-119 -0.10818267695865202 1105 120-121 -0.12240641948293529 1105 122-123 -0.2855658340560261 1105 124-125 -0.5763208587674775 1106 1 -0.12813708063098161 1106 2 -0.0927654119603929 1106 3 -0.11722820195327799 1106 4 -0.3011069063110554 1106 5 -0.05954594107313227 1106 6 -0.4108869275680149 1106 7 0.09807833568119406 1106 8 -0.2619647587127858 1106 9 -0.09534044130496966 1106 10-11 -0.1370849385970061 1106 12-13 -0.1394274877862216 1106 14-15 -0.1431355100250471 1106 16-17 -0.23663622887190883 1106 18-19 -0.19985911844034376 1106 20-21 -0.0871244146618082 1106 22-23 0.07208786791692035 1106 24-25 0.20754166226628 1106 26-27 0.17509522213956075 1106 28-29 0.21360545629027428 1106 30-31 0.05916057343898018 1106 32-33 0.03218081903927583 1106 34-35 0.12486856882944863 1106 36-37 0.12931321902360082 1106 38-39 0.22170190740398965 1106 40-41 0.07266443996699223 1106 42-43 0.1523655886084967 1106 44-45 0.20687988660971968 1106 46-47 0.1957592287756782 1106 48-49 0.21191105364854934 1106 50-51 0.14116330641204655 1106 52-53 0.10920199891337745 1106 54-55 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-0.9136934338991658 2216 124-125 -0.8030711602118394 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10752.0 3 2192.0 4 1917.0 5 1267.0 6 1170.0 7 1170.0 8 1224.0 9 1197.0 10 1427.0 11 1832.0 12 1976.0 13 1757.0 14 1712.0 15 1611.0 16 1585.0 17 1621.0 18 1363.0 19 1177.0 20 1018.0 21 915.0 22 977.0 23 1717.0 24 3365.0 25 4868.0 26 6700.0 27 3933.0 28 3379.0 29 4137.0 30 4378.0 31 4805.0 32 6380.0 33 8563.0 34 13205.0 35 23471.0 36 53495.0 37 147874.0 38 95386.0 39 481.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.91449145337285 20.734554302436457 13.350728776697935 22.000225467492754 2 14.34559555144698 22.42012386886742 40.77722656994263 22.457054009742972 3 18.78084745683117 29.503669996196162 27.252598113110082 24.462884433862584 4 11.53304708119173 19.208270759743264 37.14015779120515 32.118524367859855 5 12.419902838533044 42.1687341067743 31.976641801032663 13.434721253659992 6 28.567134315599624 39.31163079061947 18.03305637619828 14.088178517582628 7 27.218479126205615 36.22226240603446 19.86538944767872 16.69386902008121 8 25.111324687409674 38.64511687036049 19.00943939354775 17.234119048682082 9 26.260088463545216 20.92994834142604 19.082884043188262 33.727079151840485 10-11 23.36795182533867 31.59430603310599 25.55861657554065 19.479125566014694 12-13 23.98165553404305 28.878444430615907 26.140746576556793 20.999153458784246 14-15 21.847395969672174 29.536055669093813 24.455593497290145 24.16095486394386 16-17 21.154333859332734 32.62605199879772 25.158621693718064 21.060992448151488 18-19 21.219843896435172 31.335101223038315 27.04624620956774 20.398808670958772 20-21 22.92414016979015 30.046128911607035 26.70231209113812 20.327418827464697 22-23 22.267372037875706 30.283292244128628 26.32885778278108 21.120477935214584 24-25 21.568611327776264 30.29973405005122 26.834514288936294 21.297140333236225 26-27 21.463796578573884 30.457688817620593 26.780354210222956 21.298160393582567 28-29 21.38712786099376 30.331141063909957 26.94589832768539 21.335832747410887 30-31 21.665772617201668 30.158968646448237 26.886278081858933 21.28898065449116 32-33 21.31909743864233 30.250337175193764 26.85710462467031 21.573460761493596 34-35 21.689630643468707 30.16701216319957 26.86386184520973 21.279495348121994 36-37 21.739375393944087 29.921674818448263 26.740191158984977 21.598758628622672 38-39 21.485921147155356 30.217063654163084 26.825884242108376 21.471130956573184 40-41 21.6719232904626 29.93047640623001 27.000696057590844 21.39690424571655 42-43 21.3283327170602 29.852505297014304 26.898502749752023 21.920659236173474 44-45 21.416380249417774 30.125107993388927 27.013607167004732 21.444904590188564 46-47 21.481256258577112 30.112076221691865 26.727602071302876 21.67906544842815 48-49 21.34904665866835 30.071515273887535 26.94994165612094 21.629496411323174 50-51 21.460064280297782 30.155394920025262 26.840663275008392 21.543877524668563 52-53 21.626545554004736 29.932537367396073 26.82423207990806 21.61668499869113 54-55 21.570461951673035 29.889395490359966 26.955828629922145 21.58431392804485 56-57 21.466312770660597 30.126630126630126 26.691522343696256 21.715534759013018 58-59 21.454431476407485 29.85579417682705 27.029082994653912 21.660691352111552 60-61 21.433677619740184 30.22677357974521 26.869482232215486 21.470066568299124 62-63 21.253750827647416 30.028574380262313 27.128756110503254 21.588918681587018 64-65 21.16368384037271 29.958706553529435 26.98632983800805 21.891279768089806 66-67 21.307826152296375 30.22422493407566 26.533518680714874 21.934430232913094 68-69 21.38766874140662 30.073044466596922 26.798665685069267 21.74062110692719 70-71 21.25685196468971 29.962794814989618 26.955150669845956 21.82520255047472 72-73 21.21753197204163 30.07050636388609 26.81577952615403 21.896182137918245 74-75 21.010045756436774 30.32344095917136 26.709762390509706 21.95675089388216 76-77 21.099585281422886 30.172331826510764 26.895550315000648 21.8325325770657 78-79 21.228795799839627 30.195931564247452 26.756064087196528 21.81920854871639 80-81 21.12669607827024 30.486324543505773 26.567647079540464 21.819332298683523 82-83 21.332247017817373 30.382515329140684 26.680746505378554 21.604491147663385 84-85 21.235393418028224 30.349290126572768 26.701900117627446 21.71341633777156 86-87 21.034291671754218 30.429045074153038 26.787492133860557 21.74917112023218 88-89 21.18763267146319 30.209761801889805 26.81442901959311 21.788176507053894 90-91 21.239473131168925 30.33569748834457 26.675346845138648 21.749482535347855 92-93 21.32198665497245 30.010226569590575 26.867112127114734 21.800674648322243 94-95 21.02531951713758 30.199212448664753 26.8879277350033 21.88754029919437 96-97 21.22472652814908 30.199017767305016 26.655419010417585 21.920836694128322 98-99 21.00712362608654 30.014453904256683 26.71952733032514 22.258895139331646 100-101 20.891783155415773 30.270286769531307 26.82088145207966 22.01704862297326 102-103 21.01822434185963 30.209999084203925 26.787152555188452 21.98462401874799 104-105 20.689845448696577 30.42420599326568 26.66256533561255 22.223383222425202 106-107 20.642293395076656 30.571399721497528 26.740699168014952 22.04560771541086 108-109 20.715677757167345 30.63220549907253 26.43006410105896 22.22205264270117 110-111 20.503570095257327 30.86635563830861 26.51133249901972 22.118741767414342 112-113 20.529338696459725 30.91746092126489 26.533386870672103 22.019813511603278 114-115 20.327590194128828 31.180233302068306 26.395862216310956 22.096314287491914 116-117 20.337584343261224 31.215458587869595 26.496109762453692 21.95084730641549 118-119 20.145563244517604 31.254182683163368 26.478351018989677 22.121903053329355 120-121 20.1045112905536 31.30877439320759 26.483315996966333 22.103398319272483 122-123 20.23593881885022 31.327340751862753 26.41016821411759 22.02655221516944 124-125 20.25151060150526 31.191940015003293 26.46015029120241 22.096399092289033 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2418.0 1 2156.0 2 1835.0 3 1508.5 4 995.0 5 665.5 6 510.5 7 450.5 8 497.0 9 506.0 10 416.5 11 317.0 12 288.0 13 281.5 14 283.0 15 307.0 16 348.0 17 387.0 18 419.0 19 454.5 20 537.5 21 684.5 22 859.5 23 1125.0 24 1432.5 25 1788.0 26 2377.5 27 3015.0 28 3525.0 29 4245.0 30 5373.5 31 6455.0 32 7730.0 33 9214.5 34 10587.0 35 11578.0 36 12911.0 37 14232.5 38 14477.5 39 14666.0 40 14639.0 41 14429.0 42 14371.0 43 14671.0 44 15170.5 45 15382.5 46 15169.5 47 14731.0 48 14639.5 49 14584.0 50 14448.5 51 13811.0 52 12726.0 53 12195.0 54 11751.0 55 10992.5 56 10027.0 57 8942.0 58 8117.0 59 7269.0 60 6272.5 61 5265.0 62 4183.5 63 2996.5 64 2104.5 65 1627.5 66 1176.0 67 871.0 68 680.0 69 459.5 70 335.0 71 258.5 72 219.0 73 214.0 74 168.5 75 122.5 76 84.5 77 56.0 78 41.0 79 34.5 80 21.5 81 14.5 82 12.0 83 7.5 84 3.0 85 4.0 86 5.5 87 5.0 88 4.5 89 4.5 90 4.0 91 3.5 92 5.0 93 4.5 94 1.5 95 0.5 96 2.5 97 2.0 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05046983898947645 2 0.20422679032950936 3 0.02605652152479947 4 0.11690223170585709 5 0.025352291213318404 6 0.04201907525170365 7 0.041314844940222584 8 0.10328711235055646 9 0.1199538963889417 10-11 0.050000352115155744 12-13 0.034389913543992096 14-15 0.012089287013758313 16-17 0.03309882463961014 18-19 0.05962483303873032 20-21 0.20751319844975435 22-23 0.1173717185801778 24-25 0.08286443331760553 26-27 0.01490620825968258 28-29 0.0071596748333908455 30-31 0.007629161707711557 32-33 0.007394418270551201 34-35 0.009389737486414224 36-37 0.0038732667131458672 38-39 0.009154994049253868 40-41 0.006220701084749423 42-43 0.011619800139437602 44-45 0.010563454672216002 46-47 0.01913159012856898 48-49 0.017371014349866314 50-51 0.01173717185801778 52-53 0.013497747636720446 54-55 0.015258323415423113 56-57 0.016784155756965423 58-59 0.018309988098507736 60-61 0.010211339516475468 62-63 0.02136165278159236 64-65 0.03345093979535067 66-67 0.030516646830846227 68-69 0.037676321664237074 70-71 0.02711286699202107 72-73 0.018309988098507736 74-75 0.010798198109376357 76-77 0.01068082639079618 78-79 0.025469662931898583 80-81 0.020657422470111292 82-83 0.021713767937332894 84-85 0.017840501224187025 86-87 0.028403955896403026 88-89 0.031220877142327293 90-91 0.02875607105214356 92-93 0.034155170106831736 94-95 0.02816921245924267 96-97 0.030634018549426406 98-99 0.037676321664237074 100-101 0.022887485123134668 102-103 0.03239459432812907 104-105 0.027934469022082315 106-107 0.03474202869973263 108-109 0.025117547776158048 110-111 0.023004856841714848 112-113 0.029577673082204804 114-115 0.01607992544548436 116-117 0.01384986279246098 118-119 0.03145562057948765 120-121 0.025821778087639115 122-123 0.01913159012856898 124-125 0.021244281063012183 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 425997.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.78468421655177 #Duplication Level Percentage of deduplicated Percentage of total 1 83.47146217747621 58.25029629149021 2 10.287447366086798 14.35812531673529 3 2.8721667647696756 6.0129975209018065 4 1.1800237773618076 3.293903466848653 5 0.6216290928461576 2.1690094972045317 6 0.35452047477103393 1.4844059628119166 7 0.24199439370067616 1.1821251642604313 8 0.15884322125065828 0.8867859227933641 9 0.1259288223787516 0.7909112793117075 >10 0.6368026710996988 7.803184940448217 >50 0.03873864661447292 1.8806011949162513 >100 0.009768876054099087 1.1060285735885667 >500 3.368577949689341E-4 0.1194181754267783 >1k 3.368577949689341E-4 0.6622066932622725 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2817 0.6612722624807217 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 508 0.11924966607746064 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 474 0.11126838921400856 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.347434371603556E-4 0.0 14-15 0.0 0.0 0.0 2.347434371603556E-4 0.0 16-17 0.0 0.0 0.0 2.347434371603556E-4 0.0 18-19 0.0 0.0 0.0 2.347434371603556E-4 0.0 20-21 0.0 0.0 0.0 4.694868743207112E-4 0.0 22-23 0.0 0.0 0.0 5.86858592900889E-4 0.0 24-25 0.0 0.0 0.0 8.216020300612446E-4 0.0 26-27 0.0 0.0 0.0 0.0015258323415423113 0.0 28-29 0.0 0.0 0.0 0.0024648060901837337 0.0 30-31 0.0 0.0 0.0 0.007042303114810668 0.0 32-33 0.0 0.0 0.0 0.01384986279246098 0.0 34-35 0.0 0.0 0.0 0.02347434371603556 0.0 36-37 0.0 0.0 0.0 0.041314844940222584 0.0 38-39 0.0 0.0 0.0 0.07288783723829041 0.0 40-41 0.0 0.0 0.0 0.1083340962495041 0.0 42-43 0.0 0.0 0.0 0.1422545229191755 0.0 44-45 0.0 0.0 0.0 0.1822782789550161 0.0 46-47 0.0 0.0 0.0 0.23368709169313398 0.0 48-49 0.0 0.0 0.0 0.2915513489531616 0.0 50-51 0.0 0.0 0.0 0.359744317448245 0.0 52-53 0.0 0.0 0.0 0.4228903020443806 0.0 54-55 0.0 0.0 0.0 0.48556679976619554 0.0 56-57 0.0 0.0 0.0 0.5576330349744247 0.0 58-59 0.0 0.0 0.0 0.6528214987429488 0.0 60-61 0.0 0.0 0.0 0.7535264332847414 0.0 62-63 0.0 0.0 0.0 0.859513095162642 0.0 64-65 0.0 0.0 0.0 0.9604527731415948 0.0 66-67 0.0 0.0 0.0 1.0768855179731314 0.0 68-69 0.0 0.0 0.0 1.213975685274779 0.0 70-71 0.0 0.0 0.0 1.37301436395092 0.0 72-73 0.0 0.0 0.0 1.5352220790287254 0.0 74-75 0.0 0.0 0.0 1.6895658889616594 0.0 76-77 0.0 0.0 0.0 1.8554121273154505 0.0 78-79 0.0 0.0 0.0 2.068089681382733 0.0 80-81 0.0 0.0 0.0 2.301659401357287 0.0 82-83 0.0 0.0 0.0 2.5437972567881935 0.0 84-85 0.0 0.0 0.0 2.7808881283201528 0.0 86-87 0.0 0.0 0.0 3.0239649574997007 0.0 88-89 0.0 0.0 0.0 3.2923940778925673 0.0 90-91 0.0 0.0 0.0 3.613053613053613 0.0 92-93 0.0 0.0 0.0 3.9462719221027376 0.0 94-95 0.0 0.0 0.0 4.2757343361572975 0.0 96-97 0.0 0.0 0.0 4.600267138031489 0.0 98-99 0.0 0.0 0.0 4.970105423277628 0.0 100-101 0.0 0.0 0.0 5.396047390005094 0.0 102-103 0.0 0.0 0.0 5.847576391383038 0.0 104-105 0.0 0.0 0.0 6.280678032943894 0.0 106-107 0.0 0.0 0.0 6.722817296835424 0.0 108-109 0.0 0.0 0.0 7.186552956945706 0.0 110-111 0.0 0.0 0.0 7.721885365389897 0.0 112-113 0.0 0.0 0.0 8.288086535820675 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 940 0.0 55.215794 1 GTATCAA 1675 0.0 50.93228 1 TCAACGC 1980 0.0 40.33216 4 ATCAACG 2000 0.0 39.91945 3 CAACGCA 2030 0.0 39.32488 5 AACGCAG 2080 0.0 38.37957 6 TATCAAC 2235 0.0 37.89487 2 GTACATG 3475 0.0 34.507473 1 TACATGG 3610 0.0 32.87877 2 CGCAGAG 2450 0.0 31.85399 8 ACGCAGA 2510 0.0 31.567236 7 CATTCCG 80 0.005335071 29.776627 9 ACATGGG 3725 0.0 29.110846 3 GCAGAGT 2610 0.0 28.749846 9 TACAAGG 270 1.8189894E-12 28.717672 2 CATGGGA 2875 0.0 26.947432 4 GTATGGT 135 8.0865495E-5 26.514864 1 GAACAAA 730 0.0 26.151646 1 CATGGGG 855 0.0 25.789772 4 GGATATG 170 1.3675941E-5 24.56524 1 >>END_MODULE