FastQCFastQC Report
Fri 27 May 2016
SRR940048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940048_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425997
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61231.4373340657328573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45351.0645614875222127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29080.6826339152623141No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12670.29741993488217056No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11070.25986098493651366No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9480.2225367784280171No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8660.20328781658086795No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5760.13521221980436482No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.10375659922487718No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19300.071.641631
TATCACG507.376506E-659.591422
ATCACGC507.381652E-659.584413
TAACGCA902.3646862E-1159.5844084
GTATCAA38450.056.4208761
TATAACG1155.456968E-1251.8186232
TAGGACG350.00774934651.072354
TATACCG350.007778202651.024365
TCACGCA602.1727425E-549.6536754
ATAACGC1204.110916E-1044.688313
ATCAACG49250.043.5540853
TCAACGC49400.043.4218374
CAACGCA50550.042.511895
TATCAAC50750.042.3891682
AACGCAG51700.041.781836
GCACCGG758.173929E-539.6716356
GGGATCG650.001938573336.6113789
ACGCAGA58900.036.463757
CGCAGAG59750.035.945028
GTATCAC1001.0486694E-535.7590561