FastQCFastQC Report
Fri 27 May 2016
SRR940047_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940047_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469224
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39320.8379793019964878No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9990.21290471075648304No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4900.10442773600668337No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4730.10080473292073723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9450.063.7833751
GTATCAA16700.050.7446021
CAACGCA20100.040.615565
ATCAACG20150.040.2448463
TCAACGC20200.040.1581084
AACGCAG20100.040.0226336
TATCAAC22150.037.711982
GTACATG38850.035.638131
TACATGG39500.034.5910262
TAAGGTG2255.456968E-1231.7809335
GTACAAG3950.031.7278141
ACGCAGA25300.031.5611047
GTATAGG1901.038643E-931.409741
CGCAGAG25450.031.1409448
ACATGGG42650.029.7787843
GTATAGC2251.9826984E-1029.1761571
GCAGAGT26600.029.1101269
TACAAGG3700.029.0265772
CGGGTCG1254.7953763E-528.6303674
GTAAGGT2552.7284841E-1128.0689874
Produced by FastQC (version 0.11.5)