##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940047_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 469224 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.38104615279696 33.0 31.0 34.0 22.0 34.0 2 30.336274359367806 34.0 31.0 34.0 23.0 34.0 3 30.39915477469183 34.0 31.0 34.0 23.0 34.0 4 33.08860799959081 37.0 35.0 37.0 18.0 37.0 5 33.587766184167904 37.0 35.0 37.0 27.0 37.0 6 33.312714183417725 37.0 35.0 37.0 21.0 37.0 7 33.38821969890713 37.0 35.0 37.0 25.0 37.0 8 33.19443378855301 37.0 35.0 37.0 18.0 37.0 9 34.49164791229775 39.0 37.0 39.0 13.0 39.0 10-11 34.69863007859786 39.0 37.0 39.0 16.0 39.0 12-13 34.631901820878724 39.0 37.0 39.0 15.0 39.0 14-15 36.03325597156156 41.0 38.0 41.0 16.5 41.0 16-17 36.17718190032905 41.0 38.0 41.0 18.0 41.0 18-19 36.23385739007382 41.0 38.0 41.0 20.0 41.0 20-21 36.3132597650589 41.0 38.0 41.0 22.0 41.0 22-23 36.494601725401935 41.0 38.0 41.0 24.5 41.0 24-25 36.748011610659304 41.0 38.0 41.0 27.0 41.0 26-27 36.89997101597531 41.0 38.0 41.0 29.0 41.0 28-29 36.8819732153513 41.0 38.0 41.0 29.5 41.0 30-31 36.84024900687092 41.0 38.0 41.0 29.5 41.0 32-33 36.79774691831619 41.0 38.0 41.0 30.0 41.0 34-35 36.69841163282356 41.0 38.0 41.0 29.5 41.0 36-37 36.54306471962218 41.0 37.5 41.0 28.0 41.0 38-39 36.429255536801186 41.0 37.0 41.0 27.0 41.0 40-41 36.31947321535131 40.5 37.0 41.0 27.5 41.0 42-43 36.14430208173495 40.0 37.0 41.0 27.0 41.0 44-45 36.03462099125365 40.0 36.0 41.0 27.0 41.0 46-47 35.89148466404106 40.0 36.0 41.0 27.0 41.0 48-49 35.73495707806933 40.0 35.0 41.0 27.0 41.0 50-51 35.587804545377054 40.0 35.0 41.0 27.0 41.0 52-53 35.68814468143147 40.0 35.0 41.0 27.0 41.0 54-55 35.48949755340733 40.0 35.0 41.0 27.0 41.0 56-57 35.36851482447615 40.0 35.0 41.0 27.0 41.0 58-59 35.15457009871618 39.5 35.0 41.0 27.0 41.0 60-61 34.89794213424719 39.0 35.0 41.0 26.0 41.0 62-63 34.590473846180075 39.0 35.0 41.0 26.0 41.0 64-65 34.26400184133804 38.0 34.0 41.0 25.0 41.0 66-67 33.939004611869805 37.0 34.0 40.5 24.5 41.0 68-69 33.586691004722695 37.0 34.0 40.0 23.5 41.0 70-71 33.2196722674032 36.0 34.0 39.0 22.5 41.0 72-73 32.85555086696333 36.0 34.0 39.0 20.5 41.0 74-75 32.48864508209299 35.0 33.0 38.5 18.5 40.5 76-77 32.13555892281725 35.0 33.0 37.0 15.0 39.5 78-79 31.790418861780303 35.0 33.0 37.0 7.5 39.0 80-81 31.468940207662012 35.0 33.0 36.5 2.0 39.0 82-83 31.143979208224643 35.0 33.0 36.0 2.0 37.0 84-85 30.872437258111262 35.0 32.0 36.0 2.0 37.0 86-87 30.63869921402145 35.0 32.0 35.0 2.0 37.0 88-89 30.445786021175387 35.0 32.0 35.0 2.0 36.0 90-91 30.272239911001996 35.0 32.0 35.0 2.0 36.0 92-93 30.098157809489713 35.0 32.0 35.0 2.0 36.0 94-95 29.959914667621433 35.0 31.5 35.0 2.0 36.0 96-97 29.842612483589928 35.0 31.0 35.0 2.0 35.0 98-99 29.753068896731627 35.0 31.0 35.0 2.0 35.0 100-101 29.639445126421496 35.0 31.0 35.0 2.0 35.0 102-103 29.01606482191874 34.0 30.0 35.0 2.0 35.0 104-105 29.781253729562 35.0 31.5 35.0 2.0 35.0 106-107 29.846903611068488 35.0 32.0 35.0 2.0 35.0 108-109 29.807565256679112 35.0 32.0 35.0 2.0 35.0 110-111 29.738119746645523 35.0 32.0 35.0 2.0 35.0 112-113 29.6516216135577 35.0 32.0 35.0 2.0 35.0 114-115 29.550399169692938 35.0 32.0 35.0 2.0 35.0 116-117 29.451438971578604 35.0 31.0 35.0 2.0 35.0 118-119 29.34139025284299 35.0 31.0 35.0 2.0 35.0 120-121 29.22321535130343 35.0 31.0 35.0 2.0 35.0 122-123 29.059861601282115 35.0 31.0 35.0 2.0 35.0 124-125 27.916156249467207 34.0 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2510042623487543 1101 2 0.3479458153878312 1101 3 0.38685951311568445 1101 4 0.23325207205638065 1101 5 0.5091331215675012 1101 6 0.5718780281406026 1101 7 0.3855481900328357 1101 8 0.5068114440012579 1101 9 0.4186844299302521 1101 10-11 0.5753494014313247 1101 12-13 0.45153846219592 1101 14-15 0.5229440564691785 1101 16-17 0.41546052558238245 1101 18-19 0.5145589686364289 1101 20-21 0.3823479975847448 1101 22-23 0.402867912996534 1101 24-25 0.2693128348456355 1101 26-27 0.36581444292479404 1101 28-29 0.3179370743278298 1101 30-31 0.4735964066385989 1101 32-33 0.27788198313869117 1101 34-35 0.414179979770644 1101 36-37 0.5631307625462014 1101 38-39 0.5553982903586601 1101 40-41 0.6688054382817512 1101 42-43 0.7798184260417216 1101 44-45 0.6472203930349636 1101 46-47 0.7398737437969487 1101 48-49 0.7901502664940878 1101 50-51 0.7597407541720358 1101 52-53 0.7535627157759563 1101 54-55 0.8410273727471704 1101 56-57 0.804521452079733 1101 58-59 0.6660015173624743 1101 60-61 0.6959874564976118 1101 62-63 0.6479703502661209 1101 64-65 0.5138833143955281 1101 66-67 0.4846217348088757 1101 68-69 0.5227634530641581 1101 70-71 0.5599657543845638 1101 72-73 0.615490398749202 1101 74-75 0.2789562339933056 1101 76-77 0.18645530286251244 1101 78-79 0.18873694936904428 1101 80-81 0.0941574765914801 1101 82-83 0.12895649904390538 1101 84-85 0.024210882016312496 1101 86-87 0.17997836050790994 1101 88-89 0.10719778177058004 1101 90-91 0.24957816149433398 1101 92-93 0.5212549973929228 1101 94-95 0.47089668469248736 1101 96-97 0.5257605396368561 1101 98-99 0.5164158900887657 1101 100-101 0.461490631601464 1101 102-103 0.5199768051755562 1101 104-105 0.5622821473433604 1101 106-107 0.6042153055368544 1101 108-109 0.4626077899917824 1101 110-111 0.4119093432896861 1101 112-113 0.3996744590749124 1101 114-115 0.32924086807140185 1101 116-117 0.5403234712616403 1101 118-119 0.5275003770841593 1101 120-121 0.578747691764967 1101 122-123 0.6355061241109645 1101 124-125 0.48392443930191575 1102 1 0.08614460078223374 1102 2 0.019328427607913312 1102 3 0.2589210188406845 1102 4 0.06646719010278446 1102 5 -0.025064872150203144 1102 6 0.19184965647173868 1102 7 0.23648293519445218 1102 8 0.11672632899467317 1102 9 0.14222531553163265 1102 10-11 0.07897185558068287 1102 12-13 0.2355591329832336 1102 14-15 0.18052714492798572 1102 16-17 0.03819965821993776 1102 18-19 0.06253748723000996 1102 20-21 -0.057856177103680295 1102 22-23 -0.19160721112951506 1102 24-25 -0.11115884414920174 1102 26-27 -0.10138081495340856 1102 28-29 -0.25252979161602696 1102 30-31 -0.1844996517259787 1102 32-33 -0.19908503479624784 1102 34-35 -0.09263428712569777 1102 36-37 -0.24676639015417123 1102 38-39 -0.14182280600368102 1102 40-41 -0.14832395679373178 1102 42-43 0.048449999656078546 1102 44-45 0.09690501153733777 1102 46-47 0.11258121162551049 1102 48-49 0.06488048232142773 1102 50-51 0.06615477788264457 1102 52-53 0.060299720538353085 1102 54-55 0.0673547615403578 1102 56-57 -0.04426641903183537 1102 58-59 -0.04170696373996918 1102 60-61 0.07257702990930426 1102 62-63 0.06298833587763397 1102 64-65 0.0950718846660692 1102 66-67 0.06014863723060415 1102 68-69 0.0803490747076836 1102 70-71 0.17204017935162597 1102 72-73 0.11376783313971828 1102 74-75 0.21491732427600851 1102 76-77 0.09415871759551209 1102 78-79 0.07569991420844246 1102 80-81 -0.14036476477036075 1102 82-83 -0.16648250186804248 1102 84-85 -0.033590060328400284 1102 86-87 -0.0018789705286721414 1102 88-89 -7.158872941701588E-4 1102 90-91 -0.04836869229024643 1102 92-93 0.03616912243091974 1102 94-95 0.08745484661364955 1102 96-97 0.061187127943675534 1102 98-99 0.016270231967375537 1102 100-101 0.0019075938063473075 1102 102-103 0.037804092641355425 1102 104-105 -0.08039948200105229 1102 106-107 -0.017839867226342676 1102 108-109 0.06886222306504308 1102 110-111 0.06991040722726893 1102 112-113 0.02962683520217979 1102 114-115 0.18199317326949682 1102 116-117 0.1520875811005311 1102 118-119 0.11645315852097937 1102 120-121 -0.0041191133824654 1102 122-123 0.22728340651039858 1102 124-125 0.0336508553521746 1103 1 0.3595143129470202 1103 2 0.4487123226009082 1103 3 0.6439313425631212 1103 4 0.4927816211919378 1103 5 0.29095164580569843 1103 6 0.5467829209802559 1103 7 0.5448887803877724 1103 8 0.5955749881397665 1103 9 0.5449948998789012 1103 10-11 0.28149809504188283 1103 12-13 0.45212071890364314 1103 14-15 0.5475400864514697 1103 16-17 0.5649906397109774 1103 18-19 0.6723222867777281 1103 20-21 0.18323346721950173 1103 22-23 0.22031238673691433 1103 24-25 0.2821350378162393 1103 26-27 0.5180486764803547 1103 28-29 0.5237357861437175 1103 30-31 0.5261595916926467 1103 32-33 0.4937642511462812 1103 34-35 0.5626240517381973 1103 36-37 0.577693457939624 1103 38-39 0.615554353989431 1103 40-41 0.5489390277452628 1103 42-43 0.6276814968416247 1103 44-45 0.42356633199059956 1103 46-47 0.39510400984589467 1103 48-49 0.37454453118704834 1103 50-51 0.3159120475038648 1103 52-53 0.40508286091639434 1103 54-55 0.5734906168116041 1103 56-57 0.5074889210365043 1103 58-59 0.39576691970028577 1103 60-61 0.40669846883957206 1103 62-63 0.38683214041515157 1103 64-65 0.4685018185450929 1103 66-67 0.5074239119153532 1103 68-69 0.46859631556996106 1103 70-71 0.5609387979392437 1103 72-73 0.4436414904412942 1103 74-75 0.35663806758402217 1103 76-77 0.4338061133136293 1103 78-79 0.22400663937620635 1103 80-81 0.14003491076979202 1103 82-83 0.25130103179466445 1103 84-85 0.3740325953262946 1103 86-87 0.2496388624728283 1103 88-89 0.3177832401054843 1103 90-91 0.3612019514675886 1103 92-93 0.2771466191740295 1103 94-95 0.1921714973031321 1103 96-97 0.19956495160466403 1103 98-99 0.173352121208584 1103 100-101 0.2447608740462499 1103 102-103 0.2420739965324188 1103 104-105 0.27443546134137975 1103 106-107 0.3236648767699215 1103 108-109 0.49983769193410765 1103 110-111 0.4368741881677103 1103 112-113 0.4586942589418257 1103 114-115 0.2868726683903091 1103 116-117 0.32176533347867675 1103 118-119 0.48727758149863476 1103 120-121 0.45961420274466747 1103 122-123 0.48704792731761515 1103 124-125 0.44300795782741176 1104 1 -0.055754412344143134 1104 2 -0.0025289068751739308 1104 3 0.053893233078490255 1104 4 -0.014973837012284719 1104 5 0.0860063168887919 1104 6 0.25393646302534734 1104 7 0.324690753533055 1104 8 0.015571782669098866 1104 9 0.3312523347900438 1104 10-11 0.16602991824236568 1104 12-13 0.015081505312444676 1104 14-15 0.13748915508416104 1104 16-17 0.14688151124283877 1104 18-19 0.326358508596293 1104 20-21 0.34193542967988577 1104 22-23 0.2405691805539263 1104 24-25 0.3827690086820752 1104 26-27 0.35819808702991907 1104 28-29 0.21719929720259756 1104 30-31 0.2803489637765608 1104 32-33 0.4106268076872155 1104 34-35 0.408814884092088 1104 36-37 0.46354308574886005 1104 38-39 0.49577659995588164 1104 40-41 0.3255310781779954 1104 42-43 0.3530394410664428 1104 44-45 0.3021902946646762 1104 46-47 0.24445579244375182 1104 48-49 0.35697231905611915 1104 50-51 0.1272834547054913 1104 52-53 0.2503387643248729 1104 54-55 0.31831289127112683 1104 56-57 0.17022888613249165 1104 58-59 0.281745656744846 1104 60-61 0.13347216261026773 1104 62-63 0.383141397102122 1104 64-65 0.24286339563666104 1104 66-67 0.3036094018181217 1104 68-69 0.1830066870483975 1104 70-71 0.09696472663533484 1104 72-73 0.12069032044449557 1104 74-75 0.05390362302085805 1104 76-77 -0.025787148074698507 1104 78-79 0.14614041435216052 1104 80-81 0.18986662355962025 1104 82-83 0.16100025179680344 1104 84-85 0.14289144776779494 1104 86-87 0.16220074586492927 1104 88-89 0.13618887461058904 1104 90-91 0.1229508918814517 1104 92-93 0.17276848048399884 1104 94-95 0.11468369589984562 1104 96-97 0.1320408215337885 1104 98-99 0.1447254873089534 1104 100-101 0.06588670657821183 1104 102-103 0.052577255427596725 1104 104-105 0.0637870658575288 1104 106-107 0.17132488318086203 1104 108-109 0.05746047926893638 1104 110-111 -0.0013804330525033492 1104 112-113 -0.10060694845212836 1104 114-115 -0.08436090357250947 1104 116-117 -0.11423655418738932 1104 118-119 -0.10390470293346254 1104 120-121 -0.034940949159029344 1104 122-123 -0.016967815105008555 1104 124-125 -0.05691635744843637 1105 1 -0.2736462513539948 1105 2 -0.05387921288386721 1105 3 0.019231207733234612 1105 4 -0.40231372279937716 1105 5 -0.4256254261094057 1105 6 -0.16390190978196983 1105 7 -0.3650232591623279 1105 8 -0.5165818814721419 1105 9 -0.4752967062310489 1105 10-11 -0.2993137443062679 1105 12-13 -0.47340619746097445 1105 14-15 -0.2320305591560512 1105 16-17 -0.46496523414428026 1105 18-19 -0.41080675884847295 1105 20-21 -0.46694280388779674 1105 22-23 -0.4872935364392319 1105 24-25 -0.5632585662274892 1105 26-27 -0.452386514703754 1105 28-29 -0.38580561713772 1105 30-31 -0.31201390110183524 1105 32-33 -0.48752007648450757 1105 34-35 -0.44166744930949875 1105 36-37 -0.3362430494409949 1105 38-39 -0.32599893299977367 1105 40-41 -0.34533041023007627 1105 42-43 -0.37897828241090536 1105 44-45 -0.3829250558233426 1105 46-47 -0.48973755044524125 1105 48-49 -0.34544560463508844 1105 50-51 -0.39092633757262973 1105 52-53 -0.4165156028400503 1105 54-55 -0.38482739494369156 1105 56-57 -0.4097599655954909 1105 58-59 -0.3969992525901276 1105 60-61 -0.44630339309696154 1105 62-63 -0.32267219923942037 1105 64-65 -0.25216538510640873 1105 66-67 -0.10752294409683572 1105 68-69 -0.0780221391978202 1105 70-71 -0.08877344823328315 1105 72-73 -0.020670906320461313 1105 74-75 -0.038868770217369786 1105 76-77 -0.12622966621908205 1105 78-79 -0.15527245848928217 1105 80-81 -0.08432114519336764 1105 82-83 -0.1491918852586842 1105 84-85 -0.3288965985544152 1105 86-87 -0.17124634534613747 1105 88-89 -0.21058210802995347 1105 90-91 -0.2798003423915212 1105 92-93 -0.17205724244731257 1105 94-95 -0.3397761816966778 1105 96-97 -0.25947682231304725 1105 98-99 -0.1943526188499014 1105 100-101 -0.29754127330939184 1105 102-103 -0.38396860037291347 1105 104-105 -0.14323805188187322 1105 106-107 -0.374707242043808 1105 108-109 -0.27278717163089183 1105 110-111 -0.3145018354596232 1105 112-113 -0.3305173072444738 1105 114-115 -0.25851568254094914 1105 116-117 -0.4016714278023841 1105 118-119 -0.5166666304469416 1105 120-121 -0.5169700484241488 1105 122-123 -0.7249399671765424 1105 124-125 -0.7341255354340142 1106 1 0.07230373018799341 1106 2 0.16379033910987317 1106 3 -0.005386821612248127 1106 4 0.0011784229032159033 1106 5 -0.1515817990824715 1106 6 0.1268393309270337 1106 7 -0.1599106652881801 1106 8 -0.15285159986348162 1106 9 0.2409705878243713 1106 10-11 -0.10855569854141578 1106 12-13 -0.06822941378336367 1106 14-15 0.01352161090009929 1106 16-17 0.08027480266154896 1106 18-19 0.21092658623921778 1106 20-21 0.12159366859324194 1106 22-23 0.15029303714762676 1106 24-25 0.10216516912318241 1106 26-27 0.10995060022629843 1106 28-29 0.28954825325948264 1106 30-31 0.2773132354819481 1106 32-33 0.3109983236741769 1106 34-35 0.3839911160346148 1106 36-37 0.28723829694701664 1106 38-39 0.2578830899983515 1106 40-41 0.36064998570806495 1106 42-43 0.4791209280269584 1106 44-45 0.4446481839159233 1106 46-47 0.41424083990590077 1106 48-49 0.30509876897711763 1106 50-51 0.2688980082699146 1106 52-53 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-0.7159483612646369 2216 122-123 -0.7408140263500194 2216 124-125 -0.7273341589383193 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13656.0 3 2881.0 4 2599.0 5 1676.0 6 1764.0 7 1751.0 8 1790.0 9 1703.0 10 2128.0 11 2559.0 12 2908.0 13 2452.0 14 2374.0 15 2295.0 16 2180.0 17 2071.0 18 1693.0 19 1382.0 20 1155.0 21 1042.0 22 1094.0 23 2158.0 24 5083.0 25 7379.0 26 8629.0 27 4428.0 28 3445.0 29 4107.0 30 4422.0 31 5195.0 32 6855.0 33 9373.0 34 14147.0 35 25547.0 36 61264.0 37 158207.0 38 95182.0 39 650.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.98964772409852 20.291953371046464 12.96326701195576 21.755131892899254 2 14.681658179829807 22.93197430026315 39.553287652506754 22.833079867400293 3 18.74176618304761 30.022681634271436 26.609991004088663 24.62556117859229 4 11.768721997013015 19.802005547258375 35.32686153189674 33.102410923831876 5 12.504396576532406 43.516408556537314 30.218389947027912 13.76080491990237 6 28.95869622225965 39.46511682447227 17.235740463971926 14.340446489296156 7 27.28860102080411 38.37460184802214 18.000520209277635 16.336276921896122 8 25.381925703423835 39.0698101091643 17.91280083115908 17.635463356252785 9 25.987434590727887 22.297412652479366 17.74671993307554 33.96843282371721 10-11 23.329626901234423 33.04659123739751 23.884419019433714 19.73936284193435 12-13 23.898767045460602 30.356562309132944 24.712813368395135 21.03185727701132 14-15 21.891398779962742 30.476846148271612 23.418470221627313 24.21328485013833 16-17 21.180317943535922 33.63424341845795 24.051061979163336 21.13437665884279 18-19 21.41382005209361 31.765052077613714 26.350238344775235 20.470889525517443 20-21 23.333934784860077 30.137419217764243 25.948113595331314 20.580532402044366 22-23 22.799209284611667 30.48425450111428 25.622293650700517 21.09424256357354 24-25 21.85953065981901 30.310789108304842 26.311501457757036 21.518178774119107 26-27 21.62612941936281 30.60340141999373 26.12430380448336 21.64616535616009 28-29 21.74706729867517 30.555295060444337 26.13215911609746 21.56547852478303 30-31 21.831314618930804 30.44940994430863 26.10439627189664 21.614879164863925 32-33 21.466189531297623 30.43475017530249 26.001248963637252 22.09781132976263 34-35 21.92961983714157 30.33139697834842 26.146227304843233 21.592755879666782 36-37 22.036446794521368 29.98639186111813 26.06816430788707 21.908997036473433 38-39 21.786641431256953 30.20597550630672 26.053109454326894 21.954273608109435 40-41 21.94227584575349 30.090665541339778 26.237593164491674 21.72946544841506 42-43 21.57711547765963 30.05490512028612 26.200996221010158 22.166983181044092 44-45 21.701427927443085 30.339115450155752 26.281260890091467 21.678195732309696 46-47 21.82966496570364 30.448836822652225 25.842807995805146 21.878690215838994 48-49 21.556623932762577 30.171791786521606 26.188575563766737 22.083008716949077 50-51 21.888740874940055 30.340384717855812 26.02067458837321 21.750199818830925 52-53 21.9464341094359 30.188922554221847 26.14403812767699 21.720605208665265 54-55 21.75937471358446 30.18665234322546 26.231720027879557 21.82225291531052 56-57 21.74966748516472 30.409440863515446 26.0368707796194 21.80402087170043 58-59 21.643645820011084 30.15315257705589 26.02783817197425 22.175363430958775 60-61 21.517077189172138 30.355504354952327 26.23596349790112 21.891454957974418 62-63 21.376020360855232 30.340594561699124 26.28728800101463 21.99609707643101 64-65 21.356996296000215 30.42310869507288 26.049724198577024 22.17017081034988 66-67 21.27932619695011 30.61350654500068 25.798206560029502 22.30896069801971 68-69 21.56285675607398 30.21869846856709 26.09831571160412 22.12012906375481 70-71 21.30914276761133 30.25099737054434 26.15748808644482 22.282371775399508 72-73 21.31133039681381 30.451487212498364 25.935442753322768 22.301739637365063 74-75 21.146531705357212 30.799931368380218 25.814334417158758 22.239202509103816 76-77 21.287661042887578 30.587944416558233 25.916275282760886 22.20811925779331 78-79 21.265583715876232 30.64570967525332 26.059564780402432 22.029141828468013 80-81 21.242341014433904 30.840146353368997 25.907061560711814 22.01045107148529 82-83 21.35937748134032 30.805870058502343 25.96431879247921 21.87043366767812 84-85 21.3839313309844 30.649111476311365 26.018812786556083 21.948144406148153 86-87 21.341399723304065 30.67807557764007 25.90293898833315 22.077585710722715 88-89 21.42072961455733 30.554874578178925 25.93632020669499 22.08807560056875 90-91 21.27072441523974 30.662701648360436 25.868653439280326 22.1979204971195 92-93 21.30746682300272 30.502265096199967 26.048179928582847 22.142088152214466 94-95 21.094222137901706 30.54819823516684 26.113306168389254 22.244273458542196 96-97 21.259287761525563 30.44605770429878 25.991023207279497 22.303631326896163 98-99 21.130495865742425 30.506631760606396 25.77001013711217 22.592862236539013 100-101 20.84469303752273 30.771813296555955 25.991891356108876 22.391602309812438 102-103 20.9565054264293 30.620793744524004 26.05165713408642 22.371043694960274 104-105 20.57531673969417 30.915840555257713 26.111266841534757 22.397575863513357 106-107 20.478785204324183 31.24425741286723 25.800551288364748 22.476406094443842 108-109 20.521997523027107 31.313563025317563 25.612262081637788 22.552177370017542 110-111 20.48236302245869 31.729506659163388 25.63927115840453 22.148859159973398 112-113 20.45755653639252 32.010944349878116 25.457977554916273 22.073521558813088 114-115 20.13144727348685 32.23154500102844 25.397224623077552 22.239783102407166 116-117 19.988895235821712 32.38189731609999 25.543621900896905 22.085585547181395 118-119 19.92370285256253 32.520513252339114 25.316092750385323 22.239691144713028 120-121 19.899747717251472 32.577835143295964 25.448049684886374 22.07436745456619 122-123 20.02594086250703 32.54469842948007 25.298202684037314 22.13115802397558 124-125 20.05154155724566 32.60259285153985 25.239163057194503 22.106702534019988 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3311.0 1 2943.5 2 2501.5 3 2019.0 4 1313.0 5 878.0 6 669.0 7 641.0 8 756.0 9 765.5 10 578.5 11 432.5 12 408.0 13 422.5 14 438.0 15 431.0 16 442.0 17 483.5 18 569.5 19 691.5 20 825.0 21 984.0 22 1190.0 23 1493.5 24 1822.5 25 2227.5 26 2805.0 27 3511.5 28 4128.5 29 4903.5 30 5796.5 31 6873.0 32 8294.5 33 9284.0 34 10149.0 35 11006.5 36 11885.0 37 12848.5 38 13130.0 39 13220.0 40 13471.0 41 14019.5 42 14521.0 43 14761.0 44 15307.5 45 15693.0 46 15574.5 47 15703.0 48 16097.0 49 16298.5 50 16353.5 51 15850.0 52 15255.0 53 15232.5 54 15247.5 55 14332.0 56 12905.0 57 11543.5 58 10591.0 59 9670.0 60 8311.0 61 7031.0 62 5671.0 63 3869.5 64 2459.5 65 1925.0 66 1549.0 67 1268.0 68 946.0 69 622.0 70 414.5 71 268.0 72 237.5 73 213.0 74 146.5 75 109.5 76 88.0 77 61.0 78 44.5 79 29.0 80 20.5 81 13.5 82 10.0 83 8.5 84 6.0 85 7.0 86 7.0 87 5.0 88 4.0 89 4.5 90 5.0 91 2.5 92 0.5 93 2.5 94 4.5 95 5.0 96 3.5 97 1.0 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.052213868003341685 2 0.2233474843571514 3 0.02600037508737831 4 0.1116737421785757 5 0.023229843315772422 6 0.03985303394540774 7 0.0387874448024824 8 0.10250967554941777 9 0.13554293898010333 10-11 0.04677936337442245 12-13 0.03143487971629755 14-15 0.01236083405793395 16-17 0.03132832080200501 18-19 0.05551719434641025 20-21 0.19447001858387464 22-23 0.11391147937871891 24-25 0.07523059349052905 26-27 0.014278894515199562 28-29 0.006819770514722179 30-31 0.006606652686137112 32-33 0.007459124000477385 34-35 0.009270625543450463 36-37 0.004155797657408828 38-39 0.008950948800572862 40-41 0.005754181371796839 42-43 0.010869009257838473 44-45 0.010123096857790736 46-47 0.016623190629635312 48-49 0.017155985201097984 50-51 0.010442773600668337 52-53 0.013532982115151826 54-55 0.01342642320085929 56-57 0.015344483658124904 58-59 0.017901897601145723 60-61 0.0096968612006206 62-63 0.018860927829778527 64-65 0.0290905836018618 66-67 0.026746287487426048 68-69 0.03271358668780796 70-71 0.02461510920157537 72-73 0.015877278229587573 74-75 0.011188686000716076 76-77 0.01182803948647128 78-79 0.022270813087139615 80-81 0.018221574344023325 82-83 0.02077898828704414 84-85 0.01598383714388011 86-87 0.024934785944452968 88-89 0.027065964230303653 90-91 0.02589381617308578 92-93 0.031754556459175146 94-95 0.0244019913729903 96-97 0.026746287487426048 98-99 0.033139822344978094 100-101 0.02024619371558147 102-103 0.027918435544643926 104-105 0.023123284401479892 106-107 0.030049613830494603 108-109 0.021951136344262018 110-111 0.02045931154416654 112-113 0.026320051830255913 114-115 0.013319864286566759 116-117 0.011934598400763814 118-119 0.02919714251615433 120-121 0.022910166572894824 122-123 0.017049426286805447 124-125 0.018754368915485993 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 469224.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.82652198795308 #Duplication Level Percentage of deduplicated Percentage of total 1 79.35377221940456 50.648752873888455 2 11.790116199283585 15.050442216681908 3 3.977148511524626 7.615426707605441 4 1.7863381872584763 4.560630143478936 5 0.9340340802321903 2.980807337971871 6 0.5583148863519272 2.1381178421965688 7 0.35346268325290553 1.5792205607193726 8 0.22890193022091584 1.168801126586415 9 0.19022833758450144 1.092745185441205 >10 0.7761066417849039 8.408847893501934 >50 0.030811829590838512 1.3095070190700693 >100 0.020094671139242508 2.392639874246961 >500 3.3491118565404153E-4 0.2135483993900396 >1k 3.3491118565404153E-4 0.8405128192208566 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3932 0.8379793019964878 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 999 0.21290471075648304 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 490 0.10442773600668337 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 473 0.10080473292073723 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 5.327945714626703E-4 0.0 24-25 0.0 0.0 0.0 8.524713143402725E-4 0.0 26-27 0.0 0.0 0.0 0.001918060457265613 0.0 28-29 0.0 0.0 0.0 0.00340988525736109 0.0 30-31 0.0 0.0 0.0 0.009909979029205667 0.0 32-33 0.0 0.0 0.0 0.02077898828704414 0.0 34-35 0.0 0.0 0.0 0.03846776805960479 0.0 36-37 0.0 0.0 0.0 0.06681243926141886 0.0 38-39 0.0 0.0 0.0 0.11156718326428315 0.0 40-41 0.0 0.0 0.0 0.16601878846776807 0.0 42-43 0.0 0.0 0.0 0.21514244795662626 0.0 44-45 0.0 0.0 0.0 0.2646923431026546 0.0 46-47 0.0 0.0 0.0 0.32170136224916035 0.0 48-49 0.0 0.0 0.0 0.4059894634545548 0.0 50-51 0.0 0.0 0.0 0.500613779346325 0.0 52-53 0.0 0.0 0.0 0.5807460828943105 0.0 54-55 0.0 0.0 0.0 0.66482106627112 0.0 56-57 0.0 0.0 0.0 0.7615765604487409 0.0 58-59 0.0 0.0 0.0 0.880709426627794 0.0 60-61 0.0 0.0 0.0 1.0146539818935092 0.0 62-63 0.0 0.0 0.0 1.1491313317306873 0.0 64-65 0.0 0.0 0.0 1.2730593490529043 0.0 66-67 0.0 0.0 0.0 1.4226680647196221 0.0 68-69 0.0 0.0 0.0 1.6023263942168346 0.0 70-71 0.0 0.0 0.0 1.8018046817724582 0.0 72-73 0.0 0.0 0.0 2.013856921214601 0.0 74-75 0.0 0.0 0.0 2.208326939798476 0.0 76-77 0.0 0.0 0.0 2.4206988559834963 0.0 78-79 0.0 0.0 0.0 2.6801698122858166 0.0 80-81 0.0 0.0 0.0 2.976083917276184 0.0 82-83 0.0 0.0 0.0 3.28627691678175 0.0 84-85 0.0 0.0 0.0 3.579527048914804 0.0 86-87 0.0 0.0 0.0 3.886203604248717 0.0 88-89 0.0 0.0 0.0 4.2286839547849215 0.0 90-91 0.0 0.0 0.0 4.623378173324468 0.0 92-93 0.0 0.0 0.0 5.061335311066783 0.0 94-95 0.0 0.0 0.0 5.485013554293898 0.0 96-97 0.0 0.0 0.0 5.870437147289994 0.0 98-99 0.0 0.0 0.0 6.285164441716536 0.0 100-101 0.0 0.0 0.0 6.803680118664007 0.0 102-103 0.0 0.0 0.0 7.36311441869981 0.0 104-105 0.0 0.0 0.0 7.919245392392547 0.0 106-107 0.0 0.0 0.0 8.450654697969412 0.0 108-109 0.0 0.0 0.0 9.006679112747857 0.0 110-111 0.0 0.0 0.0 9.642942389988576 0.0 112-113 0.0 0.0 0.0 10.331206417404054 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 945 0.0 63.783375 1 GTATCAA 1670 0.0 50.744602 1 CAACGCA 2010 0.0 40.61556 5 ATCAACG 2015 0.0 40.244846 3 TCAACGC 2020 0.0 40.158108 4 AACGCAG 2010 0.0 40.022633 6 TATCAAC 2215 0.0 37.71198 2 GTACATG 3885 0.0 35.63813 1 TACATGG 3950 0.0 34.591026 2 TAAGGTG 225 5.456968E-12 31.780933 5 GTACAAG 395 0.0 31.727814 1 ACGCAGA 2530 0.0 31.561104 7 GTATAGG 190 1.038643E-9 31.40974 1 CGCAGAG 2545 0.0 31.140944 8 ACATGGG 4265 0.0 29.778784 3 GTATAGC 225 1.9826984E-10 29.176157 1 GCAGAGT 2660 0.0 29.110126 9 TACAAGG 370 0.0 29.026577 2 CGGGTCG 125 4.7953763E-5 28.630367 4 GTAAGGT 255 2.7284841E-11 28.068987 4 >>END_MODULE