FastQCFastQC Report
Fri 27 May 2016
SRR940047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940047_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469224
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88821.892912553492575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66311.4131843213475868No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42950.9153410737728676No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18470.3936286293966208No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15480.3299063986496854No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11100.2365607897294256No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9920.21141288595638758No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9790.20864235418478166No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5710.1216902801220739No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.082.006121
TAACGCA1000.065.6410144
GTATCAA50350.061.7609181
ATAACGC1001.8189894E-1259.673653
CCATACG150.004237557359.4955874-75
TATCACG453.1237132E-453.0432472
TGGTATC7800.051.2581372
GTGGTAT7700.050.3846361
TATAACG1207.2759576E-1249.728042
TATCAAC66300.047.0728842
ATCAACG66550.046.627043
TCAACGC67150.046.210424
CAACGCA67900.045.7778975
AACGCAG70350.044.3245626
CGCTACG355.4535958E-542.4968432-33
ACGCAGA81000.038.337517
CGCAGAG81400.037.929878
ACAACGC650.001910199836.7222483
GCAGAGT86350.035.5488669
GTACATG39750.035.136151