##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940047_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 469224 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.08951801271887 34.0 33.0 34.0 31.0 34.0 2 33.10760745400917 34.0 33.0 34.0 31.0 34.0 3 33.16815849146676 34.0 33.0 34.0 31.0 34.0 4 36.42394250933456 37.0 37.0 37.0 35.0 37.0 5 36.460232213186025 37.0 37.0 37.0 35.0 37.0 6 36.56375419842122 37.0 37.0 37.0 35.0 37.0 7 36.551293198983856 37.0 37.0 37.0 35.0 37.0 8 36.555977528856154 37.0 37.0 37.0 35.0 37.0 9 38.41002378394967 39.0 39.0 39.0 37.0 39.0 10-11 38.40115381992396 39.0 39.0 39.0 37.0 39.0 12-13 38.38502825942407 39.0 39.0 39.0 37.0 39.0 14-15 39.858778536477246 41.0 40.0 41.0 37.5 41.0 16-17 39.88006687637461 41.0 40.0 41.0 37.5 41.0 18-19 40.019656922919545 41.0 40.0 41.0 38.0 41.0 20-21 40.03750021311782 41.0 40.0 41.0 38.0 41.0 22-23 39.973048053807986 41.0 40.0 41.0 38.0 41.0 24-25 39.91969933336743 41.0 40.0 41.0 37.5 41.0 26-27 39.77774261333606 41.0 40.0 41.0 37.0 41.0 28-29 39.65464788672361 41.0 40.0 41.0 36.0 41.0 30-31 39.32042265527765 41.0 39.0 41.0 35.5 41.0 32-33 38.93826402741547 41.0 38.0 41.0 35.0 41.0 34-35 38.735665694849374 41.0 38.0 41.0 35.0 41.0 36-37 38.570988483112544 41.0 38.0 41.0 35.0 41.0 38-39 38.39908764257583 41.0 38.0 41.0 35.0 41.0 40-41 38.167743977290165 41.0 38.0 41.0 35.0 41.0 42-43 37.856876459857126 41.0 37.0 41.0 35.0 41.0 44-45 37.393320674134316 41.0 37.0 41.0 33.5 41.0 46-47 36.70873079808364 40.5 37.0 41.0 31.0 41.0 48-49 36.083446285782486 40.0 36.0 41.0 27.0 41.0 50-51 35.95576952585546 41.0 36.0 41.0 26.0 41.0 52-53 35.82904859938963 40.5 36.0 41.0 25.0 41.0 54-55 35.668179803249615 40.0 35.0 41.0 24.5 41.0 56-57 35.47442799174808 40.0 35.0 41.0 24.0 41.0 58-59 35.25651074966328 40.0 35.0 41.0 23.0 41.0 60-61 35.036442083098905 39.0 35.0 41.0 23.0 41.0 62-63 34.77652890730227 39.0 35.0 41.0 22.5 41.0 64-65 34.46900840536716 38.5 35.0 41.0 21.0 41.0 66-67 34.14218155934053 37.0 35.0 41.0 19.5 41.0 68-69 33.79468015276286 37.0 35.0 40.0 17.5 41.0 70-71 33.43526865633471 36.5 35.0 39.0 14.0 41.0 72-73 33.08217397234583 36.0 35.0 39.0 9.0 41.0 74-75 32.742431120317804 35.5 35.0 39.0 7.0 41.0 76-77 32.39610505856478 35.0 35.0 37.0 6.0 39.5 78-79 32.07050470564165 35.0 34.5 37.0 2.0 39.0 80-81 31.771258503401363 35.0 34.0 36.5 2.0 39.0 82-83 31.490400107411386 35.0 34.0 36.0 2.0 37.5 84-85 31.24557460828943 35.0 34.0 36.0 2.0 37.0 86-87 31.040510502446594 35.0 34.0 35.0 2.0 37.0 88-89 30.923499224251103 35.0 34.0 35.0 2.0 36.0 90-91 30.894974468484136 35.0 34.0 35.0 2.0 36.0 92-93 30.79573295483607 35.0 34.0 35.0 2.0 36.0 94-95 30.70361383901932 35.0 34.0 35.0 2.0 36.0 96-97 30.623503912843333 35.0 34.0 35.0 2.0 36.0 98-99 30.550860569791826 35.0 34.0 35.0 2.0 35.0 100-101 30.475600139805294 35.0 34.0 35.0 2.0 35.0 102-103 30.013609704533444 35.0 32.5 35.0 2.0 35.0 104-105 30.348642652549742 35.0 34.0 35.0 2.0 35.0 106-107 30.374616387908546 35.0 34.0 35.0 2.0 35.0 108-109 30.341048198728114 35.0 34.0 35.0 2.0 35.0 110-111 30.28092872487341 35.0 34.0 35.0 2.0 35.0 112-113 30.226452611119637 35.0 34.0 35.0 2.0 35.0 114-115 30.140993427446165 35.0 34.0 35.0 2.0 35.0 116-117 30.07030757165021 35.0 34.0 35.0 2.0 35.0 118-119 29.992247838985218 35.0 34.0 35.0 2.0 35.0 120-121 29.893870518132065 35.0 34.0 35.0 2.0 35.0 122-123 29.77521503588904 35.0 34.0 35.0 2.0 35.0 124-125 28.808534942799177 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12471988308337245 1101 2 0.015847649693519372 1101 3 0.06346024045797805 1101 4 -0.04491407032190864 1101 5 0.04867438901854371 1101 6 0.0844054180638878 1101 7 0.06427646526694275 1101 8 0.04637345559072514 1101 9 0.16545583764124672 1101 10-11 0.09532734757371486 1101 12-13 0.043022796427351295 1101 14-15 0.13685919802269098 1101 16-17 0.08816406701008361 1101 18-19 0.043107830754792076 1101 20-21 0.17830420760220989 1101 22-23 0.17333256652084827 1101 24-25 0.1453437260274626 1101 26-27 0.030462518851230413 1101 28-29 0.16719835425920593 1101 30-31 0.28361406314315474 1101 32-33 0.27073063491681637 1101 34-35 0.14439612068031238 1101 36-37 0.19805829124960894 1101 38-39 0.27098615502315937 1101 40-41 0.20066497630419633 1101 42-43 0.22084875882372046 1101 44-45 0.3975242410082487 1101 46-47 0.6438761878242616 1101 48-49 0.6149391260190953 1101 50-51 0.4382665664291565 1101 52-53 0.25077605504955613 1101 54-55 0.21458123663560968 1101 56-57 0.13744555605326525 1101 58-59 0.3363859823783031 1101 60-61 0.30455354666742096 1101 62-63 0.1872634826157764 1101 64-65 0.2665953003983006 1101 66-67 0.17013062007615076 1101 68-69 0.12357438404038135 1101 70-71 0.22668356571318782 1101 72-73 0.30368110792118586 1101 74-75 0.2278131670157606 1101 76-77 0.24084680811910175 1101 78-79 0.3671323176174468 1101 80-81 0.4014937265662546 1101 82-83 0.42579094026568853 1101 84-85 0.30384030152328734 1101 86-87 0.32985731090277426 1101 88-89 0.33401024359105946 1101 90-91 0.31597570312495904 1101 92-93 0.40309802722054755 1101 94-95 0.45960639817391424 1101 96-97 0.42930930331447925 1101 98-99 0.40354865704173193 1101 100-101 0.4465322882348417 1101 102-103 0.39405354836185325 1101 104-105 0.4218796728420884 1101 106-107 0.31454994239551226 1101 108-109 0.6258363441893522 1101 110-111 0.39529172269665835 1101 112-113 0.3187294562278673 1101 114-115 0.45534538309632566 1101 116-117 0.5154456775955722 1101 118-119 0.5068897526553435 1101 120-121 0.5322545580114664 1101 122-123 0.47700958101799884 1101 124-125 0.6509581454042284 1102 1 0.1813618934187673 1102 2 0.042509418869727256 1102 3 0.13392077344003894 1102 4 -0.01442719221876132 1102 5 0.023456535132638123 1102 6 0.08087162537972858 1102 7 0.09855045452623301 1102 8 0.167143037108616 1102 9 0.15980683571602583 1102 10-11 0.16460361669926726 1102 12-13 0.2164889530794909 1102 14-15 0.23559007247806107 1102 16-17 0.27024938467145176 1102 18-19 0.2954003629262374 1102 20-21 0.19856968536085162 1102 22-23 0.19611223011391843 1102 24-25 0.15837062844918393 1102 26-27 0.19250401241694703 1102 28-29 0.2357399998160048 1102 30-31 0.2957290190657389 1102 32-33 0.3557506470760927 1102 34-35 0.40364224490769374 1102 36-37 0.374811255075727 1102 38-39 0.4619819499365221 1102 40-41 0.5696423296327993 1102 42-43 0.6321682947442753 1102 44-45 0.43601598282606346 1102 46-47 0.625776583001084 1102 48-49 0.8173595840188952 1102 50-51 0.989139248565138 1102 52-53 0.8523694282383261 1102 54-55 0.9807176233009187 1102 56-57 0.9539859856813848 1102 58-59 0.8692287222708899 1102 60-61 0.8421016771770979 1102 62-63 0.9025525189923087 1102 64-65 0.8795816718645 1102 66-67 0.9015243783089986 1102 68-69 0.9697087441579271 1102 70-71 0.9278754291238656 1102 72-73 0.9074466870072087 1102 74-75 0.8885817352597556 1102 76-77 0.7899209291039995 1102 78-79 0.7100938638734036 1102 80-81 0.7833206593861952 1102 82-83 0.7551049538435599 1102 84-85 0.7923903065896596 1102 86-87 0.6035742528421828 1102 88-89 0.7232720735637024 1102 90-91 0.6276714169764155 1102 92-93 0.6092359337966968 1102 94-95 0.5853283558190654 1102 96-97 0.5389392050269102 1102 98-99 0.4992143779860996 1102 100-101 0.5412733967564733 1102 102-103 0.625181322199122 1102 104-105 0.6068084903514581 1102 106-107 0.6701267485562177 1102 108-109 0.5565474091402081 1102 110-111 0.6418655903630892 1102 112-113 0.6815575036490849 1102 114-115 0.5488895618378393 1102 116-117 0.5818721139113094 1102 118-119 0.530378707969966 1102 120-121 0.4870688755715058 1102 122-123 0.5004035419056265 1102 124-125 0.25604098054456514 1103 1 0.13518167072132314 1103 2 0.046162664697220634 1103 3 0.16080853982894894 1103 4 -0.11583011235739349 1103 5 -0.04025865302948972 1103 6 0.041921080926272225 1103 7 -0.03811596633751435 1103 8 0.019344816721719837 1103 9 -0.009434616487595804 1103 10-11 0.06528541253535991 1103 12-13 0.053056131016219865 1103 14-15 0.09306125208625105 1103 16-17 0.1691291378853279 1103 18-19 0.0907571458576868 1103 20-21 0.012100521336563475 1103 22-23 -0.03246143701977644 1103 24-25 -0.0048471160632601595 1103 26-27 0.021543476074981527 1103 28-29 -0.006908956750621087 1103 30-31 0.00212511817347405 1103 32-33 -0.0029818087408273186 1103 34-35 0.1919936447552928 1103 36-37 0.14202667675063196 1103 38-39 -0.1470977024981508 1103 40-41 -0.172757751482159 1103 42-43 -0.0896949528038391 1103 44-45 0.09359408670237457 1103 46-47 0.1477636537677398 1103 48-49 0.13574656383848094 1103 50-51 0.12198189782895241 1103 52-53 0.08090776120295828 1103 54-55 0.1258396644483284 1103 56-57 0.1245040767759491 1103 58-59 0.29779225003433396 1103 60-61 0.1507671516996325 1103 62-63 0.08725343829520682 1103 64-65 -0.017363919660212446 1103 66-67 0.05536013280104868 1103 68-69 0.08667406136658684 1103 70-71 -0.09469388729822725 1103 72-73 0.07127279373483475 1103 74-75 -0.08415872144205849 1103 76-77 0.07066933753012705 1103 78-79 -0.07627421370199983 1103 80-81 0.13410292716389094 1103 82-83 0.17584273129360284 1103 84-85 0.15650738251817842 1103 86-87 0.17693566915857772 1103 88-89 0.22787473315124274 1103 90-91 0.25281889872554686 1103 92-93 0.25976871161544324 1103 94-95 0.1591368261875239 1103 96-97 0.12434803672565309 1103 98-99 0.2661501988449331 1103 100-101 0.3809883882762719 1103 102-103 0.16961634419848437 1103 104-105 0.2017913455981244 1103 106-107 0.19535995407203544 1103 108-109 0.2741705564007333 1103 110-111 0.2579905688553339 1103 112-113 0.2593879242178758 1103 114-115 0.23170701630284896 1103 116-117 0.31037204017043507 1103 118-119 0.3324873897289322 1103 120-121 0.29049874799979136 1103 122-123 0.36573734226449517 1103 124-125 0.2452978568811197 1104 1 0.2360471478022106 1104 2 0.24722581246941644 1104 3 0.2489322709968107 1104 4 0.10731516669663677 1104 5 0.22407692413330693 1104 6 0.24537052353053923 1104 7 0.21719086843827995 1104 8 0.15905881750527584 1104 9 0.22289967848693948 1104 10-11 0.3004244943460961 1104 12-13 0.3166793813634854 1104 14-15 0.42661718754943934 1104 16-17 0.42455617781124033 1104 18-19 0.325090407958335 1104 20-21 0.37308103919815494 1104 22-23 0.32299854802135997 1104 24-25 0.07827697126800359 1104 26-27 0.2735557993288893 1104 28-29 0.31116277455970476 1104 30-31 0.35960633055371716 1104 32-33 0.41831053018427156 1104 34-35 0.3669099463544683 1104 36-37 0.3781407558901364 1104 38-39 0.3174061618748212 1104 40-41 0.4632120814772023 1104 42-43 0.5981367243696525 1104 44-45 0.4992285918133206 1104 46-47 0.42916836224799226 1104 48-49 0.22338502236324587 1104 50-51 0.1608660378724025 1104 52-53 0.18878075333174849 1104 54-55 0.08891442314761377 1104 56-57 0.17137963328127626 1104 58-59 0.20471784749844346 1104 60-61 0.3088725404519934 1104 62-63 0.1939377350505609 1104 64-65 0.21665769945864355 1104 66-67 0.27412905398208665 1104 68-69 0.27852593300529804 1104 70-71 0.35605761749216924 1104 72-73 0.31644232751693835 1104 74-75 0.269498186347235 1104 76-77 0.3136885347378069 1104 78-79 0.20438700366452878 1104 80-81 0.31307058752646455 1104 82-83 0.23440935165902488 1104 84-85 0.3486085685423248 1104 86-87 0.43205106610269794 1104 88-89 0.3560089711396337 1104 90-91 0.36719187304616696 1104 92-93 0.28274125141670226 1104 94-95 0.19530967228931573 1104 96-97 0.09975050137643748 1104 98-99 0.1672703327623779 1104 100-101 0.17329169703432967 1104 102-103 0.15297906101295666 1104 104-105 0.23300016420409975 1104 106-107 0.32035550202943597 1104 108-109 0.19309886070674764 1104 110-111 0.18465207115258764 1104 112-113 0.16350873484579154 1104 114-115 0.2564024965892031 1104 116-117 0.2965350917785159 1104 118-119 0.29642139264947076 1104 120-121 0.3628533442885704 1104 122-123 0.364085634460956 1104 124-125 0.43185179782922845 1105 1 0.0462990077435137 1105 2 0.06184028702178068 1105 3 0.16642937017093118 1105 4 -0.11078712438701643 1105 5 -0.08365519881620287 1105 6 0.08603555208376434 1105 7 1.2377404199526154E-4 1105 8 -0.047121647991957616 1105 9 0.10654243324770363 1105 10-11 0.08440966031884045 1105 12-13 0.10297383225420731 1105 14-15 0.18289093450641758 1105 16-17 0.10026375181073632 1105 18-19 0.07121410827458163 1105 20-21 -0.054910224196831336 1105 22-23 -0.11883136119985238 1105 24-25 -0.075145434483602 1105 26-27 -0.09054335203734354 1105 28-29 -0.019656509675733957 1105 30-31 -0.10211414358911952 1105 32-33 -0.0887680537576685 1105 34-35 -0.013829138577165168 1105 36-37 -0.06300376211773084 1105 38-39 -0.10995090135180163 1105 40-41 -0.07395546112512363 1105 42-43 0.054800660847249105 1105 44-45 0.19145665360660047 1105 46-47 0.0021838560709426247 1105 48-49 0.25985864191139996 1105 50-51 0.21106738931599267 1105 52-53 0.1361734227740996 1105 54-55 0.17123531136913073 1105 56-57 0.14753313816148506 1105 58-59 0.13913074309147078 1105 60-61 0.10412826816113352 1105 62-63 0.08399359016853225 1105 64-65 0.03142290319899388 1105 66-67 0.07106839828168177 1105 68-69 -0.07188530172271612 1105 70-71 -0.11659498870081109 1105 72-73 6.476149830945133E-4 1105 74-75 0.11720767147907907 1105 76-77 0.10382231400201647 1105 78-79 0.048666688782724066 1105 80-81 0.019925068449271777 1105 82-83 0.13636996868175189 1105 84-85 0.12990620465362568 1105 86-87 0.21430426838695382 1105 88-89 0.09839572517234885 1105 90-91 0.1665627919823045 1105 92-93 0.18537588386499948 1105 94-95 0.21702320720160628 1105 96-97 0.21893178593039053 1105 98-99 0.26046078620127844 1105 100-101 0.24184219415625918 1105 102-103 0.17077932493123527 1105 104-105 0.28415139392675925 1105 106-107 0.20900929130434776 1105 108-109 0.17726376412630884 1105 110-111 0.22503896169475723 1105 112-113 0.12567286471178463 1105 114-115 0.1133149940296505 1105 116-117 0.08702720835758626 1105 118-119 0.059057048918667476 1105 120-121 0.06180449665587062 1105 122-123 0.06397813326650237 1105 124-125 0.16417666757645932 1106 1 0.08350705433483796 1106 2 0.12146793106315101 1106 3 0.12827362862717706 1106 4 0.024435721673157218 1106 5 0.053821177683161636 1106 6 0.08435504649952463 1106 7 0.10214699256832915 1106 8 0.14445874353829424 1106 9 0.1726441561791674 1106 10-11 0.18652654636954225 1106 12-13 0.1966427340851311 1106 14-15 0.24182020450581376 1106 16-17 0.188953122750668 1106 18-19 0.1936995484843962 1106 20-21 0.20185587697511664 1106 22-23 0.23468365039839512 1106 24-25 0.25213440551023325 1106 26-27 0.2655006827188231 1106 28-29 0.23920746854932418 1106 30-31 0.2483924875206185 1106 32-33 0.32425338508570434 1106 34-35 0.28330904306069726 1106 36-37 0.43311867786617597 1106 38-39 0.5853417545632951 1106 40-41 0.6365567900360745 1106 42-43 0.5119119543957638 1106 44-45 0.38853587980265303 1106 46-47 0.3558738055455706 1106 48-49 0.11785178765255466 1106 50-51 0.22460372405067375 1106 52-53 0.18981461003752287 1106 54-55 0.22191770683341616 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-0.7982872773322534 2216 104-105 -0.70568829106832 2216 106-107 -0.674893785985585 2216 108-109 -0.6145346131581171 2216 110-111 -0.5837584923415484 2216 112-113 -0.5975223686376516 2216 114-115 -0.6115647085743952 2216 116-117 -0.7101899016923667 2216 118-119 -0.788368556236847 2216 120-121 -0.7968147276934232 2216 122-123 -0.7706427541522558 2216 124-125 -0.8038230022553385 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 2.0 4 16.0 5 46.0 6 142.0 7 294.0 8 475.0 9 787.0 10 1552.0 11 2245.0 12 2982.0 13 5315.0 14 9864.0 15 10738.0 16 3996.0 17 2776.0 18 2546.0 19 2042.0 20 1705.0 21 1620.0 22 1521.0 23 1539.0 24 1738.0 25 1960.0 26 2349.0 27 2618.0 28 3065.0 29 3413.0 30 4195.0 31 5234.0 32 6392.0 33 8423.0 34 12504.0 35 27941.0 36 52206.0 37 165547.0 38 118078.0 39 1355.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.43443265918625 18.33775280515671 14.307151870672897 19.92066266498414 2 15.902978172006804 20.699510244792354 41.523270831646116 21.874240751554726 3 19.173145448655653 28.01710909927881 30.02936763677902 22.780377815286514 4 11.689647592604844 17.559333898164013 38.00698711204348 32.744031397187655 5 11.643049946910352 38.88187660175089 32.91664712228528 16.558426329053468 6 28.455934385417464 38.39744737447886 16.969545830455875 16.1770724096478 7 25.91960343034457 37.05096073517126 19.74920293932109 17.280232895163078 8 27.04294750481646 33.511286720201866 18.77312328440148 20.67264249058019 9 28.654331406748163 15.36536920532624 19.775842667894224 36.20445672003137 10-11 24.680642933865276 28.15723407157349 25.168576202410787 21.993546792150447 12-13 27.4220841218693 26.05930216698208 27.01769304212913 19.500920669019486 14-15 23.72598162071846 25.31584062196307 28.610322575145346 22.34785518217312 16-17 19.670136224916032 29.27812729101666 28.937778118766307 22.113958365301006 18-19 19.453075716502138 24.628002830204764 36.040142874192284 19.878778579100814 20-21 20.88735870287965 23.27545053108963 37.0104683477401 18.826722418290625 22-23 20.339219647758853 23.81453207849556 36.79031763081173 19.055930642933866 24-25 19.54599508976523 23.811868105638247 37.33984195181832 19.302294852778203 26-27 19.35642678123881 24.12451196017254 37.05511653282867 19.463944725759976 28-29 19.438604417724175 24.003380052363106 37.01967186212967 19.53834366778305 30-31 19.474769432412128 23.98435710137515 37.054808752777205 19.486064713435525 32-33 19.387775761444175 23.977059972486458 36.84203796271926 19.79312630335011 34-35 19.878863309904478 23.909258304171583 36.78631862956128 19.42555975636266 36-37 19.880439878948042 23.666190699458674 36.52412514385576 19.92924427773752 38-39 19.766481569690555 23.841918444375533 36.38567517771761 20.005924808216303 40-41 20.161053143061736 23.83690945049699 36.102586397967706 19.899451008473566 42-43 20.01357575034259 23.932854381429568 35.57422014027565 20.479349727952194 44-45 20.34180055879852 24.32627695691369 35.021557006186846 20.310365478100938 46-47 20.728821126819096 24.813228663952252 33.605946846225734 20.85200336300292 48-49 20.91709841246838 25.152753831366592 32.57881714082337 21.351330615341663 50-51 21.223061447179976 25.674422867080406 31.907373150105705 21.19514253563391 52-53 21.311463181763934 25.847569604282818 31.52193834927455 21.319028864678703 54-55 21.140862359981586 25.88369307622799 31.507126660187883 21.468317903602543 56-57 21.25290774806301 25.93471512860159 31.267282652825273 21.545094470510133 58-59 21.129646699025507 25.521106949335863 31.52258813876294 21.826658212875692 60-61 21.38600820940373 25.441372666862737 31.913204523270633 21.259414600462897 62-63 21.42006954042157 25.474960226842857 32.045070206415495 21.05990002632008 64-65 21.289800093772644 25.42677208985124 32.04562891607348 21.23779890030263 66-67 21.28495953932156 25.691411935953422 31.658429609927946 21.36519891479708 68-69 21.542087843468217 25.478505863974544 31.810809831614527 21.168596460942712 70-71 21.511680567410348 25.62073826855022 31.71958965899755 21.147991505041883 72-73 21.340980001022967 25.785658875078855 31.42955177058292 21.44380935331526 74-75 21.21462373474474 26.05890369940977 31.20911463300538 21.517357932840106 76-77 21.1107191989932 25.9766692703366 31.313091486851825 21.59952004381838 78-79 21.108177170246005 25.91689204021133 31.357824990782596 21.617105798760072 80-81 20.977720662199715 26.116737421785757 31.146424735307658 21.75911718070687 82-83 21.106870066322266 25.93526759074557 31.040185497758 21.91767684517416 84-85 21.110134317940847 25.815364778969464 30.912093942639153 22.162406960450532 86-87 20.91841192780405 25.70984372036324 30.889896701568336 22.48184765026437 88-89 20.849956523962966 25.781929313078617 30.955790837638315 22.412323325320102 90-91 20.913487922066988 25.8310804978864 30.883789921007793 22.371641659038815 92-93 20.847021815969253 25.67566847569533 30.920750478719494 22.556559229615928 94-95 20.708964567755604 25.67817407418063 31.026204153919434 22.586657204144327 96-97 20.75637970939129 25.48399265166595 31.01293204495953 22.746695593983233 98-99 20.739348370927317 25.40684193476889 30.773361976369497 23.08044771793429 100-101 20.596390202526305 25.510684685107083 30.946479605645926 22.946445506720686 102-103 20.68947879903841 25.285471331389697 30.966020493410397 23.05902937616149 104-105 20.59048556766065 25.23645423081513 30.946733329923447 23.22632687160077 106-107 20.534692459662036 25.238745756282192 30.722598849587506 23.50396293446826 108-109 20.72046920221132 25.194897084961916 30.45999548188278 23.62463823094399 110-111 20.346785330673622 25.46289192368677 30.56376059195608 23.62656215368353 112-113 20.412021137048765 25.532715219932506 30.40470053183611 23.650563111182624 114-115 20.070969220031223 25.571829695184 30.36342421158631 23.993776873198463 116-117 19.8224502126399 25.55078049658956 30.67971241665699 23.947056874113557 118-119 19.685842339356764 25.627473504069492 30.509587125950777 24.177097030622967 120-121 19.708432877331248 25.709630602080463 30.4323317484437 24.14960477214459 122-123 19.74766768430755 25.78062646185978 30.18045809823698 24.291247755595695 124-125 19.558267852461846 25.655075113858928 30.149545736444022 24.637111297235204 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 890.0 1 582.5 2 634.5 3 967.5 4 561.5 5 329.5 6 982.5 7 2527.0 8 4481.0 9 4844.5 10 3066.0 11 1446.5 12 1048.5 13 1039.5 14 1100.0 15 1212.0 16 1303.5 17 1391.0 18 1513.5 19 1579.0 20 1692.0 21 1866.5 22 2037.5 23 2316.0 24 2613.0 25 2999.5 26 3508.0 27 3990.5 28 4457.5 29 5250.5 30 6133.0 31 7036.0 32 8270.5 33 9216.5 34 9818.5 35 10307.5 36 11028.0 37 12033.0 38 12414.0 39 12567.0 40 12887.5 41 13296.5 42 13560.0 43 13862.0 44 14639.0 45 15158.0 46 15147.0 47 15461.5 48 15982.5 49 16189.5 50 16197.0 51 15542.0 52 14726.5 53 14580.5 54 14466.0 55 13448.0 56 11970.5 57 10718.5 58 9841.0 59 8888.5 60 7483.5 61 6329.0 62 5298.0 63 3653.0 64 2343.5 65 1815.0 66 1394.0 67 1110.5 68 803.5 69 493.0 70 337.5 71 232.0 72 196.0 73 186.5 74 129.0 75 95.5 76 75.5 77 37.0 78 21.0 79 10.5 80 3.0 81 1.0 82 1.5 83 1.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018754368915485993 2 0.002131178285850681 3 0.0 4 0.13788723509453907 5 0.043902272688524036 6 0.012787069715104087 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.0655891429253405E-4 30-31 3.196767428776022E-4 32-33 1.0655891429253405E-4 34-35 4.262356571701362E-4 36-37 8.524713143402724E-4 38-39 0.0022377372001432154 40-41 0.0 42-43 0.0010655891429253404 44-45 6.393534857552043E-4 46-47 1.0655891429253405E-4 48-49 0.0010655891429253404 50-51 0.0017049426286805448 52-53 0.0 54-55 0.0 56-57 7.459124000477384E-4 58-59 0.0013852658858029428 60-61 0.0012787069715104087 62-63 1.0655891429253405E-4 64-65 8.524713143402724E-4 66-67 6.393534857552043E-4 68-69 6.393534857552043E-4 70-71 9.590302286328065E-4 72-73 0.0 74-75 1.0655891429253405E-4 76-77 0.0030902085144834876 78-79 6.393534857552043E-4 80-81 0.0 82-83 0.0 84-85 3.196767428776022E-4 86-87 2.131178285850681E-4 88-89 0.0 90-91 1.0655891429253405E-4 92-93 7.459124000477384E-4 94-95 9.590302286328065E-4 96-97 4.262356571701362E-4 98-99 0.0 100-101 2.131178285850681E-4 102-103 0.0 104-105 0.0 106-107 2.131178285850681E-4 108-109 4.262356571701362E-4 110-111 0.0 112-113 1.0655891429253405E-4 114-115 0.0013852658858029428 116-117 7.459124000477384E-4 118-119 2.131178285850681E-4 120-121 2.131178285850681E-4 122-123 3.196767428776022E-4 124-125 6.393534857552043E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 469224.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.09236738715085 #Duplication Level Percentage of deduplicated Percentage of total 1 76.01383184736282 42.63795782487383 2 13.559575102610896 15.21177336538628 3 4.602018072254609 7.7441426529363975 4 2.0520508391703505 4.604175582714181 5 1.114211405933143 3.1249377764277866 6 0.6808363757866897 2.2913834472697974 7 0.42216038642190384 1.65759828440353 8 0.3073093327558294 1.3790166395552137 9 0.20405842600852375 1.0301508180102468 >10 0.9605297502528763 9.354081257392062 >50 0.04875599575502058 1.8737921880539106 >100 0.031234309740242747 3.353169278191199 >500 0.0011427186490332712 0.5431219768804656 >1k 0.001523624865377695 1.8802019655972062 >5k 7.618124326888475E-4 3.314496942307893 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8882 1.892912553492575 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6631 1.4131843213475868 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4295 0.9153410737728676 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1847 0.3936286293966208 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1548 0.3299063986496854 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1110 0.2365607897294256 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 992 0.21141288595638758 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 979 0.20864235418478166 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 571 0.1216902801220739 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.1311782858506812E-4 0.0 16-17 0.0 0.0 0.0 2.1311782858506812E-4 0.0 18-19 0.0 0.0 0.0 2.1311782858506812E-4 0.0 20-21 0.0 0.0 0.0 2.1311782858506812E-4 0.0 22-23 0.0 0.0 0.0 7.459124000477385E-4 0.0 24-25 0.0 0.0 0.0 0.0010655891429253406 0.0 26-27 0.0 0.0 0.0 0.002131178285850681 0.0 28-29 0.0 0.0 0.0 0.0037295620002386915 0.0 30-31 0.0 0.0 0.0 0.01022965577208327 0.0 32-33 0.0 0.0 0.0 0.020885547201336674 0.0 34-35 0.0 0.0 0.0 0.03846776805960479 0.0 36-37 0.0 0.0 0.0 0.06649276251854125 0.0 38-39 0.0 0.0 0.0 0.11124750652140555 0.0 40-41 0.0 0.0 0.0 0.16612534738206058 0.0 42-43 0.0 0.0 0.0 0.21471621229945612 0.0 44-45 0.0 0.0 0.0 0.2645857841883621 0.0 46-47 0.0 0.0 0.0 0.3223407157349155 0.0 48-49 0.0 0.0 0.0 0.40758784716894275 0.0 50-51 0.0 0.0 0.0 0.5032777522036384 0.0 52-53 0.0 0.0 0.0 0.5840494092373791 0.0 54-55 0.0 0.0 0.0 0.6684440693570661 0.0 56-57 0.0 0.0 0.0 0.7646667689632245 0.0 58-59 0.0 0.0 0.0 0.8841193118851551 0.0 60-61 0.0 0.0 0.0 1.0191294562937958 0.0 62-63 0.0 0.0 0.0 1.1547789541881914 0.0 64-65 0.0 0.0 0.0 1.2804119141390893 0.0 66-67 0.0 0.0 0.0 1.432364925920243 0.0 68-69 0.0 0.0 0.0 1.613408521303258 0.0 70-71 0.0 0.0 0.0 1.8141655158303922 0.0 72-73 0.0 0.0 0.0 2.02600463744395 0.0 74-75 0.0 0.0 0.0 2.222605834313675 0.0 76-77 0.0 0.0 0.0 2.437854841184594 0.0 78-79 0.0 0.0 0.0 2.7007356827442757 0.0 80-81 0.0 0.0 0.0 2.998461289277616 0.0 82-83 0.0 0.0 0.0 3.3114248205547883 0.0 84-85 0.0 0.0 0.0 3.6091504270881285 0.0 86-87 0.0 0.0 0.0 3.918917190936525 0.0 88-89 0.0 0.0 0.0 4.266618928273063 0.0 90-91 0.0 0.0 0.0 4.664936149898557 0.0 92-93 0.0 0.0 0.0 5.109286822498422 0.0 94-95 0.0 0.0 0.0 5.538293011440166 0.0 96-97 0.0 0.0 0.0 5.929683903636643 0.0 98-99 0.0 0.0 0.0 6.3517637631493695 0.0 100-101 0.0 0.0 0.0 6.878484476497366 0.0 102-103 0.0 0.0 0.0 7.44633693076228 0.0 104-105 0.0 0.0 0.0 8.012804119141391 0.0 106-107 0.0 0.0 0.0 8.555508669633266 0.0 108-109 0.0 0.0 0.0 9.121229945612331 0.0 110-111 0.0 0.0 0.0 9.767296642967963 0.0 112-113 0.0 0.0 0.0 10.468028063355668 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2380 0.0 82.00612 1 TAACGCA 100 0.0 65.641014 4 GTATCAA 5035 0.0 61.760918 1 ATAACGC 100 1.8189894E-12 59.67365 3 CCATACG 15 0.0042375573 59.49558 74-75 TATCACG 45 3.1237132E-4 53.043247 2 TGGTATC 780 0.0 51.258137 2 GTGGTAT 770 0.0 50.384636 1 TATAACG 120 7.2759576E-12 49.72804 2 TATCAAC 6630 0.0 47.072884 2 ATCAACG 6655 0.0 46.62704 3 TCAACGC 6715 0.0 46.21042 4 CAACGCA 6790 0.0 45.777897 5 AACGCAG 7035 0.0 44.324562 6 CGCTACG 35 5.4535958E-5 42.49684 32-33 ACGCAGA 8100 0.0 38.33751 7 CGCAGAG 8140 0.0 37.92987 8 ACAACGC 65 0.0019101998 36.722248 3 GCAGAGT 8635 0.0 35.548866 9 GTACATG 3975 0.0 35.13615 1 >>END_MODULE