FastQCFastQC Report
Fri 27 May 2016
SRR940046_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940046_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365241
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23650.051.203851
ATCAACG27800.042.876973
CAACGCA28400.041.958795
TCAACGC28450.041.8988954
GGTATCA17400.039.4263231
TATCAAC30800.039.3114622
AACGCAG30400.039.1983456
GTACATG69750.037.6310351
TACATGG68800.036.8446432
ACATGGG73650.032.611533
ACGCAGA36800.031.8955237
CGCAGAG38350.030.295678
TATTCCG1602.5725421E-729.7907455
CATGGGG44300.029.060624
GCAGAGT46100.024.8048349
ATGGGGA24200.024.6204475
CATGGGA36750.024.327014
AGTACAT51500.024.3212552
GAGTACA54400.024.1246761
TAATACA6450.021.2531324
Produced by FastQC (version 0.11.5)