FastQCFastQC Report
Fri 27 May 2016
SRR940046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940046_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365241
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49990.36616245776386735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38630.2829537056094858No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22540.16509905577110562No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28600.068.393151
GTATCAA48100.057.2798231
ATCAACG61350.044.4114383
TCAACGC62650.043.4898874
TATCAAC63400.043.0694542
CAACGCA63950.042.6364445
AACGCAG66650.040.977536
ACGCAGA79350.034.264077
CGCAGAG80500.033.7006728
GTGGTAT12250.033.103781
GTACATG66500.030.938711
TACATGG66450.030.236262
GCAGAGT89800.029.8792639
TATCACG1254.8165613E-528.617682
TATAGCG1505.212887E-627.8227442
ACATGGG69550.027.7741683
TGGTATC13600.027.6181642
GAGTACT53700.026.25691612-13
CAGAGTA86050.026.2034210-11
GTACTTT55000.025.74446714-15
Produced by FastQC (version 0.11.5)