FastQCFastQC Report
Fri 27 May 2016
SRR940045_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940045_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058379
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12000.052.6936041
GTATCAA19900.047.962321
ATCAACG21800.041.557563
TCAACGC22300.041.1622734
CAACGCA22850.040.146785
AACGCAG24000.038.9676866
TATCAAC26050.035.261852
GTACATG53450.034.0396771
TACATGG54850.032.732712
ACGCAGA30250.030.9165127
ACATGGG57400.030.3203283
CGCAGAG30950.029.639878
GGACCGA2950.028.2697666
CATGGGA29500.026.0646084
GCAGAGT35650.024.2192279
GCCGATC1501.6733755E-423.8273758
ATGGGAG14200.023.491785
GTGGTAT7400.023.377581
CATGGGG30600.023.3745524
GGCCGAT1802.1575715E-523.1655047
Produced by FastQC (version 0.11.5)