FastQCFastQC Report
Fri 27 May 2016
SRR940045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940045_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058379
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43850.41431283122586526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32690.3088685622069221No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19670.18585024835148845No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10630.10043661108166356No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20350.073.499661
GTATCAA42150.059.802421
ATCAACG51400.048.4560553
TCAACGC52150.047.7591824
TATCAAC53050.047.288132
CAACGCA52700.047.213835
AACGCAG54800.045.7131236
TGGTATC9400.039.936332
GTGGTAT10000.039.925541
ACGCAGA65600.038.000397
CGCAGAG66000.037.6799358
TATCACG957.3967003E-637.6342352
GCAGAGT73250.033.6256379
GTACATG53850.032.644671
TACATGG54700.031.7001322
TCGACGG400.00536309629.75013114-115
ACATGGG55900.029.6324713
GAGTACT44000.029.13885312-13
GTACTTT46500.027.63621914-15
CAGAGTA71150.027.0088110-11
Produced by FastQC (version 0.11.5)