FastQCFastQC Report
Fri 27 May 2016
SRR940044_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940044_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1248486
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20700.049.2887571
ATCAACG24600.040.2215653
TCAACGC25100.039.4235084
GGTATCA13500.039.3349271
CAACGCA25150.039.081445
TATTCCG1801.4551915E-1136.403645
AACGCAG27150.036.2025156
TATCAAC27850.036.197532
GTACATG53450.034.4931531
TACATGG53400.033.7351882
ACATGGG54200.032.2221373
GTATAGG5000.032.219281
ATTCCGT2109.094947E-1131.2031176
ACGCAGA31400.030.9230677
CATGGGG33750.029.3194084
CGCAGAG33400.028.8930348
GTGTAGC7200.025.6892431
CCGTTGC2151.2235432E-724.9251069
AGTACAT38400.022.9904732
GCAGAGT40550.022.4664049
Produced by FastQC (version 0.11.5)