FastQCFastQC Report
Fri 27 May 2016
SRR940044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940044_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1248486
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35910.28762837548839154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25990.20817213809365903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16830.13480327372513587No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.058.3917661
GTATCAA42500.055.8556061
TATCACG655.3995973E-755.0434842
TCAACGC50950.046.227834
ATCAACG51000.046.1825073
CAACGCA51750.045.4347725
TATCAAC52250.045.1935962
AACGCAG54500.043.234096
ACGCAGA63300.036.8432357
CGCAGAG64450.036.0012138
GTACATG53650.032.685131
TACATGG54050.031.7734832
GCAGAGT72700.031.6702869
ACATGGG54000.030.3661353
ATAACGC1453.9997594E-628.7859543
GTGGTAT11050.027.528371
GAGTACT42450.027.32954412-13
GTACTTT43850.026.45699114-15
GTATACG1159.90708E-425.9325241
AGTACTT44750.025.65912-13
Produced by FastQC (version 0.11.5)