FastQCFastQC Report
Fri 27 May 2016
SRR940043_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940043_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794974
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15670.19711336471381455No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10050.056.9610441
GTATCAA18600.052.8985521
ATCAACG21950.045.0769653
TCAACGC22650.043.686614
CAACGCA22650.043.6590845
AACGCAG23400.042.514336
TATCAAC24500.040.162252
ACGCAGA27600.034.7497377
CGCAGAG28200.033.7991378
GTACATG45600.032.1694071
TACATGG47650.029.7861422
GCAGAGT31000.029.1997979
GTGTAGG4100.027.6339231
ACATGGG48950.027.03223
GTGGTAT6450.025.8863681
ATACCGT1200.001278596324.8212416
GGGACCG1250.001621789423.828395
TACCGTG1250.001621789423.828397
CATGGGG23900.023.444364
CAGAGTA30550.023.17666210-11
Produced by FastQC (version 0.11.5)