FastQCFastQC Report
Fri 27 May 2016
SRR940043_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940043_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794974
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58000.7295836090236913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42410.5334765665292198No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26660.33535687959606225No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13000.16352736064324117No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8740.10994070246322521No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23550.072.883951
GTATCAA43800.056.332181
TATAACG1051.10958354E-1051.0774042
GTGGTAT7900.046.772971
ATCAACG53700.045.275313
TCAACGC54100.044.9405634
TATCAAC55050.044.2732732
CAACGCA55450.043.898595
AACGCAG57550.042.5962526
ACGCAGA66550.036.6545567
CGCAGAG67300.036.157678
TGGTATC10200.035.637342
TAACGCA1555.0404196E-934.6008224
GCAGAGT71050.033.9143189
ATAACGC1451.08257154E-732.877413
GTACATG45750.031.6552331
GTATAAC2550.030.38321
TACATGG47750.029.9511492
ACATGGG46150.029.8274333
GAGTACT46850.029.3990712-13
Produced by FastQC (version 0.11.5)