FastQCFastQC Report
Fri 27 May 2016
SRR940042_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940042_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430879
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32630.7572891693491677No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6410.14876566274986713No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13150.058.486751
GGTATCA6750.058.295321
ATCAACG15550.045.9984553
TCAACGC15450.045.9371154
CAACGCA16100.044.411575
TATCAAC17700.042.7883072
AACGCAG16950.041.8329056
GTACATG31950.039.1869931
TACATGG32700.037.9323922
ACGCAGA19750.035.9021647
CGCAGAG20500.034.588678
ACATGGG35350.033.386293
GTGGTAT3600.033.122341
CATGGGG12050.032.1715164
GCAGAGT20950.031.27149
GTACAAG3700.030.6157841
TTCCGCG400.005375631629.73394874-75
ATGGGGA7100.029.3731465
CATGGGA24050.028.5185874
CGACCGT557.601649E-427.13484818-19
Produced by FastQC (version 0.11.5)