##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940042_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 430879 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.514313763260684 34.0 31.0 34.0 25.0 34.0 2 30.583049997795204 34.0 31.0 34.0 24.0 34.0 3 30.636415327737023 34.0 31.0 34.0 24.0 34.0 4 33.43795357861488 37.0 35.0 37.0 22.0 37.0 5 33.86351388672922 37.0 35.0 37.0 28.0 37.0 6 33.558722982554265 37.0 35.0 37.0 25.0 37.0 7 33.73395315158084 37.0 35.0 37.0 27.0 37.0 8 33.51556933617094 37.0 35.0 37.0 25.0 37.0 9 34.86953181751721 39.0 37.0 39.0 17.0 39.0 10-11 35.0407771091188 39.0 37.5 39.0 22.5 39.0 12-13 34.978849050429474 39.0 38.0 39.0 21.0 39.0 14-15 36.38474722601937 41.0 38.5 41.0 21.0 41.0 16-17 36.51274835858791 41.0 38.5 41.0 25.0 41.0 18-19 36.54883389536273 41.0 38.5 41.0 25.5 41.0 20-21 36.62407079481711 41.0 39.0 41.0 25.5 41.0 22-23 36.75404928065652 41.0 38.5 41.0 27.0 41.0 24-25 36.92897890126926 41.0 38.0 41.0 28.0 41.0 26-27 37.04265002471692 41.0 38.0 41.0 29.5 41.0 28-29 37.01983735573096 41.0 38.0 41.0 30.0 41.0 30-31 36.96528491757547 41.0 38.0 41.0 30.0 41.0 32-33 36.92903112010565 41.0 38.0 41.0 30.0 41.0 34-35 36.84039022556216 41.0 38.0 41.0 30.0 41.0 36-37 36.67247301446578 41.0 38.0 41.0 29.0 41.0 38-39 36.559384421148394 41.0 38.0 41.0 28.0 41.0 40-41 36.45644020711151 40.5 37.0 41.0 28.0 41.0 42-43 36.265627937309546 40.0 37.0 41.0 27.0 41.0 44-45 36.12231740233337 40.0 36.5 41.0 27.0 41.0 46-47 35.967552375492886 40.0 36.0 41.0 27.0 41.0 48-49 35.81419725723463 40.0 35.5 41.0 27.0 41.0 50-51 35.64764817965137 40.0 35.0 41.0 27.0 41.0 52-53 35.74824950856273 40.0 35.0 41.0 27.5 41.0 54-55 35.54685190041752 40.0 35.0 41.0 27.5 41.0 56-57 35.42239236537404 40.0 35.0 41.0 28.0 41.0 58-59 35.2105591128832 39.0 35.0 41.0 27.0 41.0 60-61 34.94020363025351 39.0 35.0 41.0 26.5 41.0 62-63 34.62909308645815 38.5 35.0 41.0 26.0 41.0 64-65 34.29970130825591 37.5 34.5 41.0 26.0 41.0 66-67 33.9734681894453 37.0 34.0 40.0 25.0 41.0 68-69 33.619751716839296 36.5 34.0 39.5 25.0 41.0 70-71 33.25548471844764 36.0 34.0 39.0 24.0 41.0 72-73 32.89613673444285 35.5 34.0 39.0 23.0 41.0 74-75 32.53014071235776 35.0 33.5 37.5 21.5 40.0 76-77 32.17267956897412 35.0 33.0 37.0 20.0 39.0 78-79 31.844269504895806 35.0 33.0 37.0 18.5 39.0 80-81 31.533212340355412 35.0 33.0 36.0 15.5 38.5 82-83 31.225494860506082 35.0 33.0 36.0 9.0 37.0 84-85 30.981196577229337 35.0 33.0 35.5 6.0 37.0 86-87 30.769326191343744 35.0 32.5 35.0 2.0 36.5 88-89 30.59099074218052 35.0 32.0 35.0 2.0 36.0 90-91 30.431929845733954 35.0 32.0 35.0 2.0 36.0 92-93 30.26986926724208 35.0 32.0 35.0 2.0 36.0 94-95 30.14839548922087 35.0 32.0 35.0 2.0 35.5 96-97 30.043348596705805 35.0 32.0 35.0 2.0 35.0 98-99 29.96489733776768 35.0 31.5 35.0 2.0 35.0 100-101 29.855064879003155 35.0 31.0 35.0 2.0 35.0 102-103 29.191998217597053 34.0 30.0 34.5 2.0 35.0 104-105 30.018701305935075 35.0 32.0 35.0 2.0 35.0 106-107 30.10187662893759 35.0 33.0 35.0 2.0 35.0 108-109 30.065483581237423 35.0 33.0 35.0 2.0 35.0 110-111 29.997454041621893 35.0 32.0 35.0 2.0 35.0 112-113 29.92460412319932 35.0 32.0 35.0 2.0 35.0 114-115 29.837716621139577 35.0 32.0 35.0 2.0 35.0 116-117 29.7488065094841 35.0 32.0 35.0 2.0 35.0 118-119 29.640706555668764 35.0 32.0 35.0 2.0 35.0 120-121 29.531769940052776 35.0 32.0 35.0 2.0 35.0 122-123 29.379982547304465 35.0 31.5 35.0 2.0 35.0 124-125 28.191485312581953 34.0 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4016618830338281 1101 2 0.5609478113494042 1101 3 0.5889650556664989 1101 4 0.3886399836698331 1101 5 0.5367282529997226 1101 6 0.6838589963659842 1101 7 0.6897278658309531 1101 8 0.7608406618207226 1101 9 0.8359267538511546 1101 10-11 0.6291601165685847 1101 12-13 0.6324421441884382 1101 14-15 0.7674954015692137 1101 16-17 0.6981642183717156 1101 18-19 0.5761111135543686 1101 20-21 0.32149945279663683 1101 22-23 0.4787834337225263 1101 24-25 0.5493468129550934 1101 26-27 0.6593550217264053 1101 28-29 0.5005691282751101 1101 30-31 0.7214727649047177 1101 32-33 0.7022818215795539 1101 34-35 0.8212931635984404 1101 36-37 0.7881430011489172 1101 38-39 0.6866411547457076 1101 40-41 0.8608356095127192 1101 42-43 0.8618548316275962 1101 44-45 0.8123766009071645 1101 46-47 0.8105680942331617 1101 48-49 0.9717946758484004 1101 50-51 0.9366574218009092 1101 52-53 0.9960962158290059 1101 54-55 1.0040191886150254 1101 56-57 1.0310201466983386 1101 58-59 0.9722133843806944 1101 60-61 0.9813142722782189 1101 62-63 0.9552557361924983 1101 64-65 0.9983924818883168 1101 66-67 0.8894575096326633 1101 68-69 0.9769289093758005 1101 70-71 0.9444191246520219 1101 72-73 0.9059784469488577 1101 74-75 0.8876266556172183 1101 76-77 0.94858991537442 1101 78-79 0.8956924052267006 1101 80-81 0.9590442265871211 1101 82-83 1.0345370678229742 1101 84-85 0.9486139755990486 1101 86-87 0.8973374543741137 1101 88-89 0.8488861734097597 1101 90-91 0.8892223494863352 1101 92-93 0.7240675724304779 1101 94-95 0.8771837108277474 1101 96-97 0.8208837266513314 1101 98-99 0.9669412739504395 1101 100-101 0.9317788314001803 1101 102-103 0.9940372046364594 1101 104-105 0.9974320159803653 1101 106-107 0.8434660564059442 1101 108-109 0.8743122824020908 1101 110-111 0.9385514907766606 1101 112-113 0.7853353171645523 1101 114-115 0.8254363275936818 1101 116-117 0.866085668904347 1101 118-119 0.8081529410708193 1101 120-121 0.7366783929542962 1101 122-123 0.7621277709867051 1101 124-125 0.6481658002967947 1102 1 0.2785928971847724 1102 2 0.34157133729280176 1102 3 0.44482443066650035 1102 4 0.33803562046228564 1102 5 0.45354723884877757 1102 6 0.38158894919617126 1102 7 0.6730120639441637 1102 8 0.6780650132358161 1102 9 0.5455081217756828 1102 10-11 0.5384798394459409 1102 12-13 0.5493127220186267 1102 14-15 0.3273457848239332 1102 16-17 0.49649116412643224 1102 18-19 0.6719948104883358 1102 20-21 0.5864942935985269 1102 22-23 0.6181886518829032 1102 24-25 0.6298155629550948 1102 26-27 0.4707421089905566 1102 28-29 0.5258049773317097 1102 30-31 0.5214580243386848 1102 32-33 0.6151134843154011 1102 34-35 0.6958251506267459 1102 36-37 0.6385078301583533 1102 38-39 0.674738147670233 1102 40-41 0.6406520894655472 1102 42-43 0.6004898552125013 1102 44-45 0.6449679924166034 1102 46-47 0.7471578642803252 1102 48-49 0.6426931133484004 1102 50-51 0.5691235184990191 1102 52-53 0.7293951545082464 1102 54-55 0.8144076025301175 1102 56-57 0.8033815853775792 1102 58-59 0.7527521520693767 1102 60-61 0.7605300741650112 1102 62-63 0.7539733069472163 1102 64-65 0.6973938085392604 1102 66-67 0.6133630226043607 1102 68-69 0.6799322997059889 1102 70-71 0.7333968663973067 1102 72-73 0.631428034213009 1102 74-75 0.5994301638719364 1102 76-77 0.5607435120725341 1102 78-79 0.7452428179625521 1102 80-81 0.7161565497003259 1102 82-83 0.7099534888135395 1102 84-85 0.6885019472971621 1102 86-87 0.7366136925816598 1102 88-89 0.7087660377965506 1102 90-91 0.8629804567976542 1102 92-93 0.7964732328078341 1102 94-95 0.729394795733409 1102 96-97 0.8170806606135947 1102 98-99 0.7826141807900626 1102 100-101 0.6294866733813116 1102 102-103 0.6295030065232545 1102 104-105 0.794841361499234 1102 106-107 0.7101450068776423 1102 108-109 0.7090484262700123 1102 110-111 0.7501338905408126 1102 112-113 0.7111497334381376 1102 114-115 0.6242755080182114 1102 116-117 0.6717792125364213 1102 118-119 0.8015933891840277 1102 120-121 0.908829778567501 1102 122-123 0.7659676884395346 1102 124-125 0.6290362307213258 1103 1 0.7233500303972917 1103 2 0.848233740021719 1103 3 0.9173291387644262 1103 4 0.9070971988299092 1103 5 0.8878861508339995 1103 6 0.8505403758386763 1103 7 0.8660902716689947 1103 8 0.6455915740146949 1103 9 1.0530730579942826 1103 10-11 1.0570581715356298 1103 12-13 1.0086596288871235 1103 14-15 1.205368739022127 1103 16-17 1.2534218396052381 1103 18-19 1.417244073182431 1103 20-21 1.1453117615301593 1103 22-23 1.1333984731528446 1103 24-25 1.2718117964767544 1103 26-27 1.2681819697019208 1103 28-29 1.2949429512506256 1103 30-31 1.249287324697562 1103 32-33 1.259010723415706 1103 34-35 1.28609093607961 1103 36-37 1.4702599532440175 1103 38-39 1.2702503531920328 1103 40-41 1.3107991953178058 1103 42-43 1.1844698857707243 1103 44-45 1.3280383829222302 1103 46-47 1.2777640540966217 1103 48-49 1.1907157122184557 1103 50-51 1.2287617944468607 1103 52-53 1.2049077153581962 1103 54-55 1.2760019599191637 1103 56-57 1.2322839767359994 1103 58-59 1.3506948021066876 1103 60-61 1.298713636685001 1103 62-63 1.3030885398648167 1103 64-65 1.2867837101133617 1103 66-67 1.231760137339819 1103 68-69 1.442009903372977 1103 70-71 1.5431328575851708 1103 72-73 1.2819752395571484 1103 74-75 1.3684135700475366 1103 76-77 1.3700051401860946 1103 78-79 1.3005336540496764 1103 80-81 1.2230765653347682 1103 82-83 1.2438322508502822 1103 84-85 1.3255413531885019 1103 86-87 1.2600191575285358 1103 88-89 1.2579665494690815 1103 90-91 1.136318409396882 1103 92-93 1.414863240980381 1103 94-95 1.2709131128993114 1103 96-97 1.2974168010392795 1103 98-99 1.2932988981265225 1103 100-101 1.2969617115790868 1103 102-103 1.21533252007902 1103 104-105 1.4399840257496699 1103 106-107 1.360933835360747 1103 108-109 1.4324094751986998 1103 110-111 1.3963496457907851 1103 112-113 1.4338445420939294 1103 114-115 1.4838178453667545 1103 116-117 1.3703578999542536 1103 118-119 1.3814889227092344 1103 120-121 1.2410793610569328 1103 122-123 1.0526872989998886 1103 124-125 1.1583525793693 1104 1 0.032645614307234894 1104 2 -0.09073026683411811 1104 3 -0.02408942701140404 1104 4 -0.07453592925151753 1104 5 -0.25144180786170267 1104 6 -0.10520350363401576 1104 7 -0.0028341660042556782 1104 8 -0.09716641917178492 1104 9 -0.1360126188080244 1104 10-11 -0.19661308684901968 1104 12-13 -0.22380931882654664 1104 14-15 -0.10632321383340582 1104 16-17 0.03435774599343233 1104 18-19 -0.12405493864600459 1104 20-21 -0.1392954032426914 1104 22-23 -0.04135130995724978 1104 24-25 0.08974065658804875 1104 26-27 0.11636432650168871 1104 28-29 0.18151482115900563 1104 30-31 0.20012737632794142 1104 32-33 0.33634519938853913 1104 34-35 0.17751902385124652 1104 36-37 0.27922226730060373 1104 38-39 0.13891029098166285 1104 40-41 0.009364547949047619 1104 42-43 0.059990950541454424 1104 44-45 0.04729291645023892 1104 46-47 0.18911956310020628 1104 48-49 -0.07172636230703233 1104 50-51 -0.22100438765601638 1104 52-53 -0.12362230008859143 1104 54-55 0.029625608324757025 1104 56-57 -0.06461714262750462 1104 58-59 -0.04400779422417145 1104 60-61 -0.029541298882826084 1104 62-63 -0.1976732198000093 1104 64-65 -0.12589429830831023 1104 66-67 -0.05241324762388899 1104 68-69 0.11842074575220352 1104 70-71 0.1685639923954696 1104 72-73 0.32199407194885765 1104 74-75 0.24703457345429314 1104 76-77 0.23185156097367354 1104 78-79 0.16460187385965597 1104 80-81 0.20716425233618807 1104 82-83 0.2707371790121158 1104 84-85 0.16241898496234342 1104 86-87 0.09749984903703535 1104 88-89 0.1291815561344798 1104 90-91 0.16180032818483525 1104 92-93 0.27579978216830625 1104 94-95 0.24191334565920997 1104 96-97 0.21733152627679786 1104 98-99 0.1650005845777791 1104 100-101 0.15142858561366523 1104 102-103 0.007409161565298916 1104 104-105 0.07346124701969003 1104 106-107 0.12451854938347395 1104 108-109 0.11245454597887061 1104 110-111 0.16691715674107854 1104 112-113 0.23737402854095535 1104 114-115 0.20698697717233117 1104 116-117 0.26874762466277247 1104 118-119 0.37593442515321485 1104 120-121 0.33242175086628123 1104 122-123 0.2125827686458841 1104 124-125 0.13141062122376113 1105 1 0.3146667341168623 1105 2 0.4623396773241346 1105 3 0.3520446472729972 1105 4 0.26605142320051556 1105 5 -0.07133526234323995 1105 6 0.0879372905898137 1105 7 0.25891220698618866 1105 8 0.3931525413333574 1105 9 0.5642943080027791 1105 10-11 0.5290254426064962 1105 12-13 0.47244806702237696 1105 14-15 0.5133106373544152 1105 16-17 0.5927448253211764 1105 18-19 0.3776926512348737 1105 20-21 0.20033871836522366 1105 22-23 0.34622829495899055 1105 24-25 0.4512957073594208 1105 26-27 0.5809832369791152 1105 28-29 0.5842785145567007 1105 30-31 0.6504410636411819 1105 32-33 0.7483741613810011 1105 34-35 0.7226250961345357 1105 36-37 0.8268635279991017 1105 38-39 0.8077842616951685 1105 40-41 0.6602454414170467 1105 42-43 0.5828752513026814 1105 44-45 0.6134680945894715 1105 46-47 0.4800915881411072 1105 48-49 0.47662813139893956 1105 50-51 0.5455318013135511 1105 52-53 0.6101389730492244 1105 54-55 0.5834662497612371 1105 56-57 0.3041615050015878 1105 58-59 0.5797939067182369 1105 60-61 0.6352797506175705 1105 62-63 0.6536565710300408 1105 64-65 0.5379802808504124 1105 66-67 0.5607508027283359 1105 68-69 0.489349233157391 1105 70-71 0.6228832774047319 1105 72-73 0.5061095954145642 1105 74-75 0.46391487199808523 1105 76-77 0.41675468297369633 1105 78-79 0.2553988018873561 1105 80-81 0.3933924328145615 1105 82-83 0.48026205090059193 1105 84-85 0.11978387632106902 1105 86-87 0.08278561547519914 1105 88-89 0.0905554254404457 1105 90-91 0.11012642778056048 1105 92-93 0.2752859839828261 1105 94-95 0.10594612057503738 1105 96-97 -0.020638159630259167 1105 98-99 0.09428572904971588 1105 100-101 0.057829429324367965 1105 102-103 -0.0666859451830355 1105 104-105 0.15891737811935513 1105 106-107 0.18966275090052775 1105 108-109 0.20636468538043218 1105 110-111 0.04412953116474583 1105 112-113 -0.12815957791667643 1105 114-115 -0.007479822000181713 1105 116-117 0.028614577974742872 1105 118-119 0.05644660081810926 1105 120-121 0.2033941815373268 1105 122-123 0.1539725927376061 1105 124-125 0.2637068934827127 1106 1 0.01805720646040143 1106 2 0.11706942411164079 1106 3 0.0790901649322322 1106 4 0.20029317422927306 1106 5 0.15394306943328928 1106 6 0.08885244042193108 1106 7 0.10313477142535987 1106 8 0.27732612947806246 1106 9 0.18836337972528128 1106 10-11 0.38958816508257144 1106 12-13 0.08664614331431153 1106 14-15 0.36784914938390045 1106 16-17 0.3008057174975889 1106 18-19 0.4045980562991289 1106 20-21 0.4794089261357968 1106 22-23 0.5722261238448993 1106 24-25 0.5333120664516002 1106 26-27 0.45414031455857184 1106 28-29 0.41097122617721027 1106 30-31 0.5241661652543712 1106 32-33 0.4922047892369008 1106 34-35 0.5753837995250137 1106 36-37 0.5733607131244369 1106 38-39 0.5569290699554941 1106 40-41 0.49051354876097264 1106 42-43 0.533921046662563 1106 44-45 0.7224782180400311 1106 46-47 0.659009691698202 1106 48-49 0.7283574490127336 1106 50-51 0.6849501447030093 1106 52-53 0.5902013294653656 1106 54-55 0.624682704786359 1106 56-57 0.4806213267682722 1106 58-59 0.6414182029995743 1106 60-61 0.5499877862642322 1106 62-63 0.4276934547239648 1106 64-65 0.48073923669601015 1106 66-67 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10-11 -0.3730215656122624 2216 12-13 -0.24467324651571687 2216 14-15 -0.42118894018610575 2216 16-17 -0.3676463977630391 2216 18-19 -0.469441025186768 2216 20-21 -0.3947027777631362 2216 22-23 -0.5789524847805154 2216 24-25 -0.6089888949983049 2216 26-27 -0.6565743254113912 2216 28-29 -0.5664901422416051 2216 30-31 -0.7823385002928518 2216 32-33 -0.7687656233292657 2216 34-35 -0.8233973838306383 2216 36-37 -0.8216821872376059 2216 38-39 -0.6564063534863109 2216 40-41 -0.8395021801909266 2216 42-43 -0.6723753165486954 2216 44-45 -0.6350295224464304 2216 46-47 -0.9811985043610605 2216 48-49 -1.0857769981004282 2216 50-51 -0.9714625914969659 2216 52-53 -1.0880246998244942 2216 54-55 -0.827215574239581 2216 56-57 -0.8911912578102772 2216 58-59 -0.9499337075012733 2216 60-61 -0.8692667180966538 2216 62-63 -0.7504378111732564 2216 64-65 -0.79654819218716 2216 66-67 -0.8227335918744316 2216 68-69 -0.7890789981090052 2216 70-71 -0.931037000284654 2216 72-73 -0.8814000516580336 2216 74-75 -0.9442413392536011 2216 76-77 -0.933591331459418 2216 78-79 -0.8573292988006536 2216 80-81 -0.8767923520602956 2216 82-83 -0.9984988443477434 2216 84-85 -0.9042624808345892 2216 86-87 -0.9445069130271015 2216 88-89 -0.8825809064408006 2216 90-91 -0.8582951831251115 2216 92-93 -1.04714570011258 2216 94-95 -1.1119167830932248 2216 96-97 -1.0585726436627496 2216 98-99 -1.0969924029112619 2216 100-101 -1.1124939807832064 2216 102-103 -1.009311009649256 2216 104-105 -0.9500067583990663 2216 106-107 -1.0026486694045929 2216 108-109 -0.9233166594672078 2216 110-111 -0.9380741345399564 2216 112-113 -0.9193658369007984 2216 114-115 -0.8574262373125983 2216 116-117 -1.0055314869340535 2216 118-119 -1.0261579441875384 2216 120-121 -0.9498232771951471 2216 122-123 -1.0664662164753018 2216 124-125 -0.9736888175476821 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12387.0 3 2613.0 4 2252.0 5 1371.0 6 1508.0 7 1467.0 8 1411.0 9 1416.0 10 1740.0 11 2135.0 12 2306.0 13 2135.0 14 1981.0 15 1982.0 16 1822.0 17 1831.0 18 1446.0 19 1234.0 20 1032.0 21 941.0 22 995.0 23 1864.0 24 4077.0 25 5676.0 26 7085.0 27 4064.0 28 3307.0 29 4096.0 30 4227.0 31 5001.0 32 6627.0 33 9170.0 34 13907.0 35 25295.0 36 61360.0 37 151114.0 38 77609.0 39 395.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.844953860014954 19.83856810466137 12.970746274202014 22.345731761121666 2 14.837449420957165 22.2626854564904 39.43351472024557 23.46635040230687 3 18.895277979613205 28.942890505119912 26.578746381471273 25.583085133795606 4 12.288450636158329 19.109610684114166 34.39878623407281 34.2031524456547 5 13.26972851886672 41.92555451095094 30.484580475179037 14.320136495003307 6 30.141718828358627 38.73609279518565 16.595311948587455 14.526876427868274 7 28.023672101003438 36.7217065458142 18.161767095872474 17.092854257309888 8 25.900069463965856 38.39847225739304 17.884299517469373 17.817158761171733 9 26.055325130993456 21.52916710234336 17.843226098776622 34.572281667886564 10-11 24.03590286582053 31.5998393171443 23.966010467605685 20.398247349429482 12-13 24.528325979478858 29.025611379843124 24.838847853568332 21.607214787109687 14-15 22.153591892491015 29.417574548438353 23.131400000696363 25.297433558374273 16-17 22.003094613213975 32.538128397429126 23.759804154860667 21.69897283449623 18-19 22.02834345101891 30.838566318729733 25.937148449608355 21.195941780643004 20-21 23.51364613080565 29.436155270085486 25.564402853873847 21.485795745235016 22-23 23.235495023280453 29.633437421585302 25.29948606425591 21.83158149087834 24-25 22.604472255708778 29.60970544990837 25.711405483123933 22.074416811258917 26-27 22.230424074448056 29.75914409803225 25.600456827693783 22.409974999825906 28-29 22.3049919054886 29.763779527559052 25.650491183075218 22.280737383877124 30-31 22.378583257609254 29.525891832360234 25.729823449745325 22.365701460285187 32-33 21.9736057244848 29.61058463366686 25.66560133317551 22.750208308672835 34-35 22.672121159369834 29.646792584210985 25.56880494385934 22.11228131255984 36-37 22.732262758814368 29.323166047361205 25.341236924376652 22.603334269447778 38-39 22.41747478170449 29.341074835553037 25.69910267896518 22.542347703777292 40-41 22.57266033433328 29.22822510922985 25.727183581578593 22.471930974858275 42-43 22.352512458423565 29.32563197340965 25.535068112238385 22.786787455928398 44-45 22.277683901696268 29.49367264894575 25.698538627944327 22.530104821413648 46-47 22.4076397488855 29.730651962223725 25.453604286913052 22.40810400197772 48-49 22.41825764091279 29.42655287559237 25.634561659464772 22.520627824030065 50-51 22.35096686140841 29.695562255847474 25.622233518326382 22.331237364417735 52-53 22.6940534869335 29.510544537046602 25.437339185799956 22.358062790219943 54-55 22.281696614060458 29.447367871293906 25.749458579284184 22.521476935361445 56-57 22.33952173368059 29.7748870721392 25.503823066531105 22.381768127649103 58-59 22.462975858867225 29.14403435468895 25.64032033426184 22.752669452181987 60-61 22.168012496605442 29.50547422807233 25.815331552304006 22.51118172301822 62-63 22.06702840727737 29.685633868206484 25.772567681281373 22.474770043234773 64-65 21.911279042096893 29.569860644367445 25.682877423923202 22.835982889612463 66-67 21.851824767529457 30.020998088233757 25.28998603609721 22.837191108139574 68-69 22.08304795514435 29.457181663048654 25.800239137248525 22.65953124455847 70-71 21.892581754614856 29.470314366270422 25.81023341547583 22.826870463638883 72-73 21.83238356100788 29.710309768920972 25.585937953366372 22.871368716704772 74-75 21.870118536158518 30.03059147384522 25.472508291960573 22.626781698035696 76-77 22.02080386042404 29.767880167164346 25.62057035954725 22.590745612864367 78-79 21.921904145062683 29.86474079284437 25.60174851514339 22.61160654694956 80-81 21.874593756383828 30.162311735101305 25.450350065927534 22.512744442587334 82-83 22.09827076693077 29.99542678326435 25.432888624761762 22.47341382504312 84-85 21.903671987529844 29.96632918473915 25.475030090171856 22.65496873755915 86-87 21.91947941599129 29.949715262091203 25.535291017697165 22.595514304220345 88-89 21.922106323753717 30.131099663138638 25.290487787324633 22.65630622578301 90-91 21.856648523408055 30.15309752901122 25.476942506880622 22.5133114407001 92-93 21.923005039277577 29.837491714387536 25.56646889543354 22.673034350901347 94-95 21.812996305242887 29.695376968534788 25.769508909553323 22.72211781666901 96-97 22.105694473141096 29.68420270643597 25.537839726431276 22.672263093991656 98-99 21.589704716388663 29.840743961004968 25.416075120466473 23.153476202139895 100-101 21.44824520371933 29.990377840945364 25.7719490269316 22.789427928403708 102-103 21.558772010266136 30.031701515891967 25.652574137552776 22.756952336289125 104-105 21.272757874209965 30.052523751762884 25.795952479063043 22.878765894964104 106-107 21.34523765300247 30.30841954716841 25.548394286669513 22.797948513159604 108-109 21.5114133096857 30.38531092070228 25.19204028145015 22.911235488161868 110-111 21.20768242390758 30.949067437679258 25.3072656174736 22.53598452093956 112-113 21.252441958860175 31.028750982297176 25.290626476347676 22.42818058249497 114-115 21.055264309332504 31.23372983311727 25.089975546149535 22.621030311400695 116-117 20.84701365653133 31.22966810926618 25.37013760983428 22.55318062436821 118-119 20.867581763835283 31.508517368466876 25.038451493078735 22.585449374619113 120-121 20.768796835390145 31.5597403682759 25.145903557400338 22.525559238933614 122-123 20.835485379818312 31.49516645523415 25.066766637688996 22.602581527258547 124-125 20.903345617038564 31.539099904245134 24.994341757827236 22.56321272088907 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2748.0 1 2394.5 2 2070.5 3 1795.5 4 1206.0 5 771.0 6 570.0 7 542.5 8 621.5 9 597.5 10 470.0 11 395.5 12 363.0 13 381.5 14 411.5 15 399.0 16 403.0 17 424.5 18 469.0 19 560.5 20 649.0 21 730.0 22 874.5 23 1175.5 24 1445.5 25 1704.5 26 2118.5 27 2610.0 28 3113.0 29 3738.5 30 4474.5 31 5319.5 32 6613.0 33 7344.0 34 7738.0 35 8258.0 36 8944.5 37 10001.0 38 10569.5 39 10767.5 40 11118.0 41 11785.0 42 12379.0 43 12952.5 44 13872.0 45 15048.0 46 15525.0 47 15708.5 48 15843.0 49 15682.5 50 15745.0 51 15503.0 52 15074.5 53 15224.5 54 15304.5 55 14562.5 56 13324.5 57 12022.0 58 11305.0 59 10275.5 60 8654.0 61 7326.5 62 5943.5 63 4289.5 64 3086.0 65 2378.5 66 1707.5 67 1311.0 68 955.5 69 617.0 70 477.5 71 351.5 72 270.0 73 232.0 74 164.5 75 135.5 76 98.0 77 58.5 78 42.0 79 25.0 80 18.0 81 12.5 82 10.5 83 6.5 84 5.0 85 8.5 86 8.5 87 5.0 88 2.5 89 0.0 90 1.5 91 1.5 92 1.0 93 1.5 94 2.5 95 3.5 96 1.5 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05407550611656636 2 0.19935991310785625 3 0.02552920889623305 4 0.11209643542618693 5 0.02181586942041733 6 0.03875798077882654 7 0.03736547847539565 8 0.1021168355849322 9 0.11929103066057989 10-11 0.050362166640750654 12-13 0.03539276687886854 14-15 0.015897734630836036 16-17 0.030286925099621935 18-19 0.04583653415460025 20-21 0.20121658284576413 22-23 0.11023976568827908 24-25 0.08308597077137665 26-27 0.019030864813555547 28-29 0.007310637093012192 30-31 0.008935223113681567 32-33 0.006498344082677503 34-35 0.009631474265397014 36-37 0.004641674344769645 38-39 0.009515432406777774 40-41 0.004989799920627368 42-43 0.010675850992970184 44-45 0.011836269579162595 46-47 0.01845065552045934 48-49 0.020771492692844163 50-51 0.012068353296401078 52-53 0.014737316044643624 54-55 0.015781692772216794 56-57 0.016942111358409204 58-59 0.0183346136618401 60-61 0.009747516124016255 62-63 0.02123566012732113 64-65 0.03400026457543765 66-67 0.02912650651342952 68-69 0.038061729627111095 70-71 0.028662339078952558 72-73 0.01717419507564769 74-75 0.00928334868953929 76-77 0.010559809134350943 78-79 0.02634150190656774 80-81 0.02390462287556367 82-83 0.02552920889623305 84-85 0.021003576410082644 86-87 0.03063505067547966 88-89 0.03202755297891055 90-91 0.03260776227200676 92-93 0.03771360405125337 94-95 0.030983176251337383 96-97 0.02970671580652573 98-99 0.03655318546506096 100-101 0.02378858101694443 102-103 0.033071929706483726 104-105 0.028198171644475595 106-107 0.03875798077882654 108-109 0.02552920889623305 110-111 0.024020664734182913 112-113 0.029822757665144972 114-115 0.015665650913597555 116-117 0.013808981175689695 118-119 0.03283984598924524 120-121 0.026689627482425458 122-123 0.020307325258367197 124-125 0.02123566012732113 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 430879.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.06173332089622 #Duplication Level Percentage of deduplicated Percentage of total 1 80.57047357786114 52.420546654611165 2 11.295823197105797 14.698516729801813 3 3.595210866478446 7.017319518816266 4 1.585501183781583 4.126218207966505 5 0.8521228950371871 2.7720296276769756 6 0.5191562111538731 2.0266321777188105 7 0.33450388524337227 1.523438180355557 8 0.2364752487204246 1.2308391655392696 9 0.18093527637478593 1.0594766429855078 >10 0.7819448886945534 8.764154236896758 >50 0.027497487015170942 1.1635692343072397 >100 0.01964106209392914 2.290196069116057 >500 3.571102198896207E-4 0.14893128541172695 >1k 3.571102198896207E-4 0.7581322687963574 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3263 0.7572891693491677 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 641 0.14876566274986713 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.1604185861924113E-4 0.0 18-19 2.3208371723848226E-4 0.0 0.0 4.6416743447696453E-4 0.0 20-21 2.3208371723848226E-4 0.0 0.0 5.802092930962057E-4 0.0 22-23 2.3208371723848226E-4 0.0 0.0 0.0010443767275731702 0.0 24-25 4.6416743447696453E-4 0.0 0.0 0.0011604185861924113 0.0 26-27 4.6416743447696453E-4 0.0 0.0 0.0029010464654810283 0.0 28-29 6.962511517154468E-4 0.0 0.0 0.004525632486150405 0.0 30-31 9.283348689539291E-4 0.0 0.0 0.00916730683092005 0.0 32-33 9.283348689539291E-4 0.0 0.0 0.01624586020669376 0.0 34-35 9.283348689539291E-4 0.0 0.0 0.02738587863414091 0.0 36-37 9.283348689539291E-4 0.0 0.0 0.04641674344769645 0.0 38-39 0.0011604185861924113 0.0 0.0 0.08157742660932651 0.0 40-41 0.0011604185861924113 0.0 0.0 0.12451291429844573 0.0 42-43 0.0012764604448116524 0.0 0.0 0.16373506251174924 0.0 44-45 0.0013925023034308936 0.0 0.0 0.20852721993877632 0.0 46-47 0.0015085441620501347 0.0 0.0 0.2605139726001963 0.0 48-49 0.0016245860206693758 0.0 0.0 0.31969532049600935 0.0 50-51 0.0016245860206693758 0.0 0.0 0.3941941937295621 0.0 52-53 0.0016245860206693758 0.0 0.0 0.4652118112045377 0.0 54-55 0.001740627879288617 0.0 0.0 0.5355331775277978 0.0 56-57 0.0018566697379078581 0.0 0.0 0.6146737251061203 0.0 58-59 0.0018566697379078581 0.0 0.0 0.7061147096980823 0.0 60-61 0.0018566697379078581 0.0 0.0 0.8165865591035999 0.0 62-63 0.0018566697379078581 0.0 0.0 0.9218365248712516 0.0 64-65 0.0018566697379078581 0.0 0.0 1.0164106396459331 0.0 66-67 0.0018566697379078581 0.0 0.0 1.1389508423478518 0.0 68-69 0.0018566697379078581 0.0 0.0 1.2873683795218611 0.0 70-71 0.0023208371723848226 0.0 0.0 1.4552809489439031 0.0 72-73 0.0023208371723848226 0.0 0.0 1.6212208067694178 0.0 74-75 0.0023208371723848226 0.0 0.0 1.770450636953762 0.0 76-77 0.0023208371723848226 0.0 0.0 1.9363904947792767 0.0 78-79 0.0023208371723848226 0.0 0.0 2.1326172777044134 0.0 80-81 0.002785004606861787 0.0 0.0 2.3611036973256994 0.0 82-83 0.0030170883241002694 0.0 0.0 2.6132626561053103 0.0 84-85 0.0030170883241002694 0.0 0.0 2.8530051360126625 0.0 86-87 0.0030170883241002694 0.0 0.0 3.1051640947922734 0.0 88-89 0.0030170883241002694 0.0 0.0 3.4011868761299575 0.0 90-91 0.003481255758577234 0.0 0.0 3.7288890848706946 0.0 92-93 0.003481255758577234 0.0 0.0 4.081888418790426 0.0 94-95 0.003481255758577234 0.0 0.0 4.436512338730827 0.0 96-97 0.003481255758577234 0.0 0.0 4.770364765978384 0.0 98-99 0.0038293813344349573 0.0 0.0 5.134620160184182 0.0 100-101 0.004177506910292681 0.0 0.0 5.596234673771523 0.0 102-103 0.004525632486150405 0.0 0.0 6.064927740734637 0.0 104-105 0.0048737580620081275 0.0 0.0 6.536289770445995 0.0 106-107 0.00510584177924661 0.0 0.0 6.9665729822061415 0.0 108-109 0.005337925496485092 0.0 0.0 7.432248960845156 0.0 110-111 0.005337925496485092 0.0 0.0 7.965925468635046 0.0 112-113 0.005337925496485092 0.0 0.0 8.545554552438155 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 1315 0.0 58.48675 1 GGTATCA 675 0.0 58.29532 1 ATCAACG 1555 0.0 45.998455 3 TCAACGC 1545 0.0 45.937115 4 CAACGCA 1610 0.0 44.41157 5 TATCAAC 1770 0.0 42.788307 2 AACGCAG 1695 0.0 41.832905 6 GTACATG 3195 0.0 39.186993 1 TACATGG 3270 0.0 37.932392 2 ACGCAGA 1975 0.0 35.902164 7 CGCAGAG 2050 0.0 34.58867 8 ACATGGG 3535 0.0 33.38629 3 GTGGTAT 360 0.0 33.12234 1 CATGGGG 1205 0.0 32.171516 4 GCAGAGT 2095 0.0 31.2714 9 GTACAAG 370 0.0 30.615784 1 TTCCGCG 40 0.0053756316 29.733948 74-75 ATGGGGA 710 0.0 29.373146 5 CATGGGA 2405 0.0 28.518587 4 CGACCGT 55 7.601649E-4 27.134848 18-19 >>END_MODULE