FastQCFastQC Report
Fri 27 May 2016
SRR940042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940042_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430879
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72451.681446531392804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53391.2390949663362567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36600.8494264050928451No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14820.3439480689474307No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12630.2931217348722031No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11150.25877334472090774No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9090.21096409896978036No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6020.1397143977775663No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5490.12741396076392675No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.083.51521
ATAACGC850.077.1719743
TAACGCA950.075.325764
TATAACG900.072.893132
GTATCAA39750.059.42861
GTATAAC1800.049.711431
GTGGTAT5700.049.1881521
TGGTATC6050.046.3317682
TATCAAC52950.044.6031572
ATCAACG53150.044.317963
TCAACGC53800.043.7825134
CAACGCA54900.042.9339875
AACGCAG57200.041.188496
CCTATAC905.0696653E-639.755263
ACGCAGA64000.036.7192577
CGCAGAG65200.035.860948
GCAGAGT67850.034.1972779
TAAGGTG2650.033.6917655
GTACATG31900.033.0993961
TACATGG33200.031.6162952
Produced by FastQC (version 0.11.5)