##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940042_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 430879 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.099705485762826 34.0 33.0 34.0 31.0 34.0 2 33.12251235265585 34.0 33.0 34.0 31.0 34.0 3 33.18250135188765 34.0 33.0 34.0 31.0 34.0 4 36.43579520004456 37.0 37.0 37.0 35.0 37.0 5 36.46582683305522 37.0 37.0 37.0 35.0 37.0 6 36.56223440919609 37.0 37.0 37.0 35.0 37.0 7 36.553210994269854 37.0 37.0 37.0 35.0 37.0 8 36.554580288201564 37.0 37.0 37.0 35.0 37.0 9 38.40770610774719 39.0 39.0 39.0 37.0 39.0 10-11 38.40073199204417 39.0 39.0 39.0 37.0 39.0 12-13 38.38041074176277 39.0 39.0 39.0 37.0 39.0 14-15 39.884298144026516 41.0 40.0 41.0 37.5 41.0 16-17 39.90474704035239 41.0 40.0 41.0 38.0 41.0 18-19 40.02308652777229 41.0 40.0 41.0 38.0 41.0 20-21 40.039677032299096 41.0 40.0 41.0 38.0 41.0 22-23 39.97604199786947 41.0 40.0 41.0 38.0 41.0 24-25 39.93150165127565 41.0 40.0 41.0 38.0 41.0 26-27 39.805409407281395 41.0 40.0 41.0 37.0 41.0 28-29 39.685996532669265 41.0 40.0 41.0 36.5 41.0 30-31 39.38383397659204 41.0 39.0 41.0 35.5 41.0 32-33 39.04079451539759 41.0 38.5 41.0 35.0 41.0 34-35 38.856594310699755 41.0 38.0 41.0 35.0 41.0 36-37 38.698910831115 41.0 38.0 41.0 35.0 41.0 38-39 38.54093956772087 41.0 38.0 41.0 35.0 41.0 40-41 38.33809955927302 41.0 38.0 41.0 35.0 41.0 42-43 38.064397429440746 41.0 38.0 41.0 35.0 41.0 44-45 37.67394326481448 41.0 37.5 41.0 33.5 41.0 46-47 37.05931015435888 40.5 37.0 41.0 32.5 41.0 48-49 36.52173696095656 40.0 37.0 41.0 30.5 41.0 50-51 36.4345732792733 41.0 37.0 41.0 30.0 41.0 52-53 36.313169126367264 40.5 36.0 41.0 30.0 41.0 54-55 36.144444263934886 40.0 35.5 41.0 30.0 41.0 56-57 35.9525330777318 40.0 35.0 41.0 29.0 41.0 58-59 35.73095114869835 40.0 35.0 41.0 28.5 41.0 60-61 35.49424432381249 39.0 35.0 41.0 28.0 41.0 62-63 35.217275615660085 39.0 35.0 41.0 28.0 41.0 64-65 34.895181709946414 38.5 35.0 41.0 27.0 41.0 66-67 34.571161509379664 37.0 35.0 41.0 26.0 41.0 68-69 34.22146472675624 37.0 35.0 40.0 26.0 41.0 70-71 33.85903002931217 36.0 35.0 39.0 25.5 41.0 72-73 33.50450706578877 36.0 35.0 39.0 25.0 41.0 74-75 33.15423239470942 35.0 35.0 38.0 24.0 40.5 76-77 32.81013463176437 35.0 35.0 37.0 23.5 39.0 78-79 32.486254841846545 35.0 35.0 37.0 22.0 39.0 80-81 32.194565063509714 35.0 34.5 36.0 20.0 39.0 82-83 31.937451117367054 35.0 34.0 36.0 20.0 37.0 84-85 31.711863423374076 35.0 34.0 36.0 19.0 37.0 86-87 31.512540643660984 35.0 34.0 35.0 18.0 36.5 88-89 31.409301683303198 35.0 34.0 35.0 17.5 36.0 90-91 31.386605056175863 35.0 34.0 35.0 16.5 36.0 92-93 31.293526720958784 35.0 34.0 35.0 13.0 36.0 94-95 31.214604332074664 35.0 34.0 35.0 9.0 36.0 96-97 31.142022470345502 35.0 34.0 35.0 7.0 35.5 98-99 31.071805541695 35.0 34.0 35.0 3.5 35.0 100-101 31.001908888574285 35.0 34.0 35.0 2.0 35.0 102-103 30.535729288268865 35.0 33.5 35.0 2.0 35.0 104-105 30.88344639678425 35.0 34.0 35.0 2.0 35.0 106-107 30.910601352119734 35.0 34.0 35.0 2.0 35.0 108-109 30.876495489452957 35.0 34.0 35.0 2.0 35.0 110-111 30.824356721956747 35.0 34.0 35.0 2.0 35.0 112-113 30.77710099587123 35.0 34.0 35.0 2.0 35.0 114-115 30.698840045581242 35.0 34.0 35.0 2.0 35.0 116-117 30.621148860817073 35.0 34.0 35.0 2.0 35.0 118-119 30.53920706277168 35.0 34.0 35.0 2.0 35.0 120-121 30.448993801043912 35.0 34.0 35.0 2.0 35.0 122-123 30.338542839172945 35.0 34.0 35.0 2.0 35.0 124-125 29.342528877016516 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2902618240693613 1101 2 0.2657633029414015 1101 3 0.311953564189281 1101 4 -0.06404161292185506 1101 5 0.10005269292120289 1101 6 0.1647344390339498 1101 7 0.1536692502778152 1101 8 0.22323950580386764 1101 9 0.27070704165489445 1101 10-11 0.3165839036594349 1101 12-13 0.3108411065914751 1101 14-15 0.3812664212919046 1101 16-17 0.4017158701350283 1101 18-19 0.4114210369979503 1101 20-21 0.3311279063113517 1101 22-23 0.30308475173806215 1101 24-25 0.22939512435294063 1101 26-27 0.27738050136493086 1101 28-29 0.2773761812627171 1101 30-31 0.34075085716188624 1101 32-33 0.44622935190550095 1101 34-35 0.4950218800230246 1101 36-37 0.43602259470301163 1101 38-39 0.4722853368528206 1101 40-41 0.6357917160870201 1101 42-43 0.5228001122216526 1101 44-45 0.3052355861623042 1101 46-47 0.4025087805367775 1101 48-49 0.6139231185165599 1101 50-51 0.5788725172639602 1101 52-53 0.5661580190464832 1101 54-55 0.5718810617875434 1101 56-57 0.4520250598326925 1101 58-59 0.3981543707311772 1101 60-61 0.42006087345910004 1101 62-63 0.5616343043202576 1101 64-65 0.47429643088081264 1101 66-67 0.3307225442888466 1101 68-69 0.39622635251475913 1101 70-71 0.4408139851926336 1101 72-73 0.4777983577783118 1101 74-75 0.2345903678852892 1101 76-77 0.1929972646013951 1101 78-79 0.13745738024906018 1101 80-81 0.19684729517739186 1101 82-83 0.199675102987662 1101 84-85 0.20156234215875202 1101 86-87 0.08134329574053822 1101 88-89 0.1678286353746259 1101 90-91 0.22435878507508278 1101 92-93 0.24318245610063371 1101 94-95 0.19642609818358991 1101 96-97 0.2921047932298215 1101 98-99 0.26716742772789814 1101 100-101 0.3210096552418378 1101 102-103 0.45244353832308093 1101 104-105 0.49146821192267254 1101 106-107 0.37091619997656977 1101 108-109 0.3693134026690643 1101 110-111 0.38397817539894064 1101 112-113 0.48082623045330664 1101 114-115 0.43213747174890216 1101 116-117 0.572784914888711 1101 118-119 0.5574597535091996 1101 120-121 0.6259311261018468 1101 122-123 0.6850997188831656 1101 124-125 0.5580193176322545 1102 1 0.11029425331464182 1102 2 0.151966133130081 1102 3 0.20394206654777491 1102 4 0.12315620547437334 1102 5 0.16896483914761973 1102 6 0.1679626229962139 1102 7 0.16490893188159106 1102 8 0.11699687608688691 1102 9 0.15450715957942407 1102 10-11 0.2377034496499988 1102 12-13 0.2096360653178877 1102 14-15 0.3092513859145427 1102 16-17 0.3027034880595565 1102 18-19 0.22531033534700384 1102 20-21 0.19167846645286346 1102 22-23 0.29414459843617635 1102 24-25 0.2887848649189806 1102 26-27 0.27240924546870815 1102 28-29 0.27679761404574066 1102 30-31 0.30988411187886555 1102 32-33 0.44555128586776505 1102 34-35 0.26765601917396253 1102 36-37 0.46621864423131143 1102 38-39 0.5965298828433845 1102 40-41 0.368198850520983 1102 42-43 0.2708580426461822 1102 44-45 0.1319491769641914 1102 46-47 0.4868358736971601 1102 48-49 0.5154008602618418 1102 50-51 0.4168036788205569 1102 52-53 0.3143265037162948 1102 54-55 0.41656708773093953 1102 56-57 0.3353940162006168 1102 58-59 0.4807641879481608 1102 60-61 0.3795464277043834 1102 62-63 0.3484046463013897 1102 64-65 0.29454259833364915 1102 66-67 0.1075208933454519 1102 68-69 0.20800037963739726 1102 70-71 -7.507258922743176E-4 1102 72-73 -0.10403020943866892 1102 74-75 0.10399263793246405 1102 76-77 0.022670024035356562 1102 78-79 0.1149374804849117 1102 80-81 0.035795555790599565 1102 82-83 0.020732001572568493 1102 84-85 0.1896814459323366 1102 86-87 0.1404529655518587 1102 88-89 0.14701495612934323 1102 90-91 0.1233711671505553 1102 92-93 0.04370574619497347 1102 94-95 0.11150569724019377 1102 96-97 -0.048195914317350486 1102 98-99 -0.02665258996078279 1102 100-101 -0.004023511031746807 1102 102-103 0.08555821992685253 1102 104-105 0.07606432041323785 1102 106-107 0.09552188983506227 1102 108-109 0.028544682150197787 1102 110-111 0.0655893192668664 1102 112-113 -0.031043610348579165 1102 114-115 0.09379357434324476 1102 116-117 0.08885581701135337 1102 118-119 0.23391538440542803 1102 120-121 0.16211921572448773 1102 122-123 0.1480861575624104 1102 124-125 0.40015891079263 1103 1 0.09102836126333358 1103 2 -0.010543194233740394 1103 3 0.14479769672223597 1103 4 0.1164440568050722 1103 5 0.13760713030350047 1103 6 0.0554550722731193 1103 7 0.15034047791434801 1103 8 0.07426866658541087 1103 9 0.051714280355461995 1103 10-11 0.09050876535906127 1103 12-13 0.16330660506134365 1103 14-15 0.25799885420151014 1103 16-17 0.23070156928756802 1103 18-19 0.21256826804786044 1103 20-21 0.14884543514845205 1103 22-23 0.1890936088472941 1103 24-25 0.15935481126442852 1103 26-27 0.227991450635173 1103 28-29 0.3097756192335268 1103 30-31 0.3622168411543569 1103 32-33 0.3890701004930719 1103 34-35 0.30224366086106613 1103 36-37 0.41222830915687325 1103 38-39 0.31845007614190024 1103 40-41 0.4402842714071724 1103 42-43 0.5847318300888844 1103 44-45 0.6435915036528925 1103 46-47 0.7644750734981756 1103 48-49 0.8476415595476325 1103 50-51 0.8049623683703615 1103 52-53 0.9524372239664416 1103 54-55 0.8673914490285952 1103 56-57 0.9241976263345748 1103 58-59 0.9793304388808934 1103 60-61 1.0896590667265187 1103 62-63 1.0044860875123476 1103 64-65 1.1179853727358022 1103 66-67 0.8462445546466597 1103 68-69 0.8238853243838733 1103 70-71 0.8563238574854779 1103 72-73 0.6597387562011008 1103 74-75 0.65522029667531 1103 76-77 0.6914597763716372 1103 78-79 0.6438629681727903 1103 80-81 0.6030733868675924 1103 82-83 0.6054653277993438 1103 84-85 0.5984402847199561 1103 86-87 0.698442518904379 1103 88-89 0.6034748218153041 1103 90-91 0.7635463439263077 1103 92-93 0.7049181258275681 1103 94-95 0.6719807709707872 1103 96-97 0.5758118318362229 1103 98-99 0.5089264172523791 1103 100-101 0.5288372358680178 1103 102-103 0.5362440032477522 1103 104-105 0.6108847020356656 1103 106-107 0.7027617008004867 1103 108-109 0.7687127523630366 1103 110-111 0.8406452834497884 1103 112-113 0.8667302588195973 1103 114-115 1.0281697957837395 1103 116-117 0.8670759783303303 1103 118-119 0.8858661359244522 1103 120-121 0.7974015239361236 1103 122-123 0.550058228770169 1103 124-125 0.7112256058855486 1104 1 0.1366598811854658 1104 2 0.07674176736087901 1104 3 0.24584723226044503 1104 4 0.0777318538384435 1104 5 0.16034354029947906 1104 6 0.07813858229237525 1104 7 0.15003365804935243 1104 8 0.0668944098300841 1104 9 0.1595032729283048 1104 10-11 0.1110339563279723 1104 12-13 0.19389866160083358 1104 14-15 0.24303345078628524 1104 16-17 0.2532052193859684 1104 18-19 0.18773701897360695 1104 20-21 0.26569090373644144 1104 22-23 0.17779946381671152 1104 24-25 0.15318667397841068 1104 26-27 0.17157891545669202 1104 28-29 0.19542173954923925 1104 30-31 0.14444350697461772 1104 32-33 0.1787609530290979 1104 34-35 0.18893720071590536 1104 36-37 0.3123319931112505 1104 38-39 0.2161150711692983 1104 40-41 0.39930265943907983 1104 42-43 0.25712756423476435 1104 44-45 0.4011033003476996 1104 46-47 0.23441201672591916 1104 48-49 -0.059169841455350536 1104 50-51 -0.06362879944951771 1104 52-53 -0.09513572510145707 1104 54-55 -0.07959219429859843 1104 56-57 0.08374395613419239 1104 58-59 0.04996777780046813 1104 60-61 -0.01880704554839241 1104 62-63 0.09094580947006392 1104 64-65 0.19246853996321533 1104 66-67 0.028961074251391494 1104 68-69 -0.029565870097599145 1104 70-71 0.10023507016454403 1104 72-73 0.08556769883636406 1104 74-75 0.05962474873734891 1104 76-77 0.06950417003211129 1104 78-79 -0.1585873587490667 1104 80-81 -0.26308438202298134 1104 82-83 -0.06545978699361399 1104 84-85 0.021523718563244643 1104 86-87 0.008748492369754501 1104 88-89 -0.0992008590073965 1104 90-91 -0.10091333571143224 1104 92-93 0.07163663447142099 1104 94-95 0.036503930580593646 1104 96-97 -0.017498437107256848 1104 98-99 -0.11079152007172866 1104 100-101 -0.03624031549786366 1104 102-103 -0.050665368512127884 1104 104-105 0.05164201810244862 1104 106-107 0.14566675592226375 1104 108-109 -0.03771565280846545 1104 110-111 -0.09598583443252195 1104 112-113 -0.03315653523021567 1104 114-115 -0.06703958232413143 1104 116-117 0.020390690871860073 1104 118-119 -0.033419079590050416 1104 120-121 -0.060091228767067406 1104 122-123 -0.01616725209061798 1104 124-125 0.0784466643082844 1105 1 0.12920304699355256 1105 2 0.13027142568508054 1105 3 0.011278079080980774 1105 4 -0.10344961156806676 1105 5 0.04329220104394693 1105 6 0.1520849579870216 1105 7 0.060828828346409125 1105 8 -0.01572569049577055 1105 9 0.04080237107727669 1105 10-11 0.032199737368820536 1105 12-13 0.025660178116744703 1105 14-15 0.16545668528107882 1105 16-17 0.21499147934080298 1105 18-19 0.18309931429939752 1105 20-21 0.15762765247561106 1105 22-23 0.2530438559799393 1105 24-25 0.30240110359481776 1105 26-27 0.31742661485770896 1105 28-29 0.3937140366327512 1105 30-31 0.40181245463481474 1105 32-33 0.3872548482953917 1105 34-35 0.2916359932901784 1105 36-37 0.2865203947932642 1105 38-39 0.23398645937086826 1105 40-41 0.3034995793541668 1105 42-43 0.32321823609348854 1105 44-45 0.18225569559371024 1105 46-47 0.28779970449887315 1105 48-49 0.24929160339923584 1105 50-51 0.21589093445710716 1105 52-53 0.28740054499414214 1105 54-55 0.3286711678344787 1105 56-57 0.32404601538323163 1105 58-59 0.3936311588629451 1105 60-61 0.29412309141938664 1105 62-63 0.4740398210170085 1105 64-65 0.4930168167111333 1105 66-67 0.4320594125198909 1105 68-69 0.3882128992205338 1105 70-71 0.2665028671872207 1105 72-73 0.25241689906892617 1105 74-75 0.19125849175712517 1105 76-77 0.29714042795880147 1105 78-79 0.4265375077310125 1105 80-81 0.43270652915754226 1105 82-83 0.2496877947746654 1105 84-85 0.329975022664172 1105 86-87 0.2722559762459511 1105 88-89 0.16619280504971812 1105 90-91 0.1258846199126289 1105 92-93 0.1772627246024392 1105 94-95 0.2309900647900882 1105 96-97 0.1500977986449854 1105 98-99 -0.026799037750443233 1105 100-101 0.03438327324725421 1105 102-103 0.10638786368409114 1105 104-105 0.0259757705869319 1105 106-107 -0.0783695344548363 1105 108-109 -0.041488013170287275 1105 110-111 0.00388030761915914 1105 112-113 0.017205827700596643 1105 114-115 -0.09268554833232656 1105 116-117 -0.048982204921760086 1105 118-119 0.04341065039547942 1105 120-121 0.07193462339426304 1105 122-123 0.06998521076042152 1105 124-125 0.1505812253488621 1106 1 0.13884683281062138 1106 2 0.14622658965468815 1106 3 0.17240756331515428 1106 4 0.18487938795227166 1106 5 0.10193752683728974 1106 6 0.07330633588709645 1106 7 0.11262630884424851 1106 8 0.18356238604862085 1106 9 0.12218759235419441 1106 10-11 0.19970371353706184 1106 12-13 0.1742521096509151 1106 14-15 0.2882129903478514 1106 16-17 0.267237067687482 1106 18-19 0.20665022187557724 1106 20-21 0.279506675979178 1106 22-23 0.2944117840807223 1106 24-25 0.3157970037235671 1106 26-27 0.29582592313066414 1106 28-29 0.3257057813501305 1106 30-31 0.2751367837353129 1106 32-33 0.11750339036704105 1106 34-35 0.1808965522258248 1106 36-37 0.3681549155072048 1106 38-39 0.30503364323394067 1106 40-41 0.20499790052758016 1106 42-43 0.054972041854519205 1106 44-45 -0.10380751916986242 1106 46-47 0.07234187712768403 1106 48-49 0.1904569362613131 1106 50-51 0.3420048927010271 1106 52-53 0.21580427123179646 1106 54-55 0.4100721675567698 1106 56-57 0.5102158924375857 1106 58-59 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-0.40665873203003855 2216 26-27 -0.42032937547143945 2216 28-29 -0.49607616011265776 2216 30-31 -0.4343647076810768 2216 32-33 -0.4978325148624805 2216 34-35 -0.45749165531132263 2216 36-37 -0.3961649844509836 2216 38-39 -0.45442498324596414 2216 40-41 -0.5976037531379035 2216 42-43 -0.4365275932621344 2216 44-45 -0.37746471303982077 2216 46-47 -0.3770225881942224 2216 48-49 -0.10278173995608597 2216 50-51 -0.1877508125866001 2216 52-53 -0.24604416402089413 2216 54-55 -0.21241438844041483 2216 56-57 -0.13704199152242325 2216 58-59 -0.0677906093220173 2216 60-61 -0.36877801648517305 2216 62-63 -0.2632315296463119 2216 64-65 -0.08753669665211561 2216 66-67 -0.06638516847711173 2216 68-69 -0.0985326631260719 2216 70-71 -0.13781463214525758 2216 72-73 -0.17594147093242896 2216 74-75 -0.07811246424490292 2216 76-77 0.06036286110595057 2216 78-79 -0.13910373455590275 2216 80-81 -0.11100339662858971 2216 82-83 0.026286282698904984 2216 84-85 -0.08308336452817855 2216 86-87 -0.26010332280049653 2216 88-89 -0.2990374993771461 2216 90-91 -0.30024623645480375 2216 92-93 -0.31705967599156537 2216 94-95 -0.40642265434933833 2216 96-97 -0.3054525142676461 2216 98-99 -0.3439262035410984 2216 100-101 -0.3842475556877467 2216 102-103 -0.4452756391085302 2216 104-105 -0.3294498161928274 2216 106-107 -0.2767544956288006 2216 108-109 -0.24008237525647047 2216 110-111 -0.3026926864298325 2216 112-113 -0.27917784597779516 2216 114-115 -0.18910963913002377 2216 116-117 -0.28667418623843943 2216 118-119 -0.22461245868684898 2216 120-121 -0.2177674237968361 2216 122-123 -0.2968412955545219 2216 124-125 -0.35503693711698503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 5.0 4 9.0 5 45.0 6 113.0 7 275.0 8 381.0 9 666.0 10 1175.0 11 1739.0 12 2329.0 13 3947.0 14 7746.0 15 7976.0 16 3102.0 17 2178.0 18 2165.0 19 1743.0 20 1491.0 21 1361.0 22 1299.0 23 1314.0 24 1511.0 25 1786.0 26 2031.0 27 2322.0 28 2780.0 29 3222.0 30 3836.0 31 4772.0 32 6025.0 33 7811.0 34 11631.0 35 26700.0 36 51683.0 37 160925.0 38 101921.0 39 858.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.23362867601828 18.07478661742483 14.079419868662649 20.61216483789424 2 16.132618483962307 20.158520168964397 41.11474724968667 22.594114097386623 3 19.442349244219375 26.942830817932645 29.72365791788414 23.89116201996384 4 12.20872127886059 16.917756322950417 36.75864248892525 34.11487990926375 5 12.429966216608426 38.284128771608025 32.496604246723244 16.78930076506031 6 29.75656065325677 37.46338428971325 16.62457697539146 16.155478081638527 7 26.722119202838847 35.662216074582425 19.655170012927062 17.960494709651663 8 27.268444273218233 33.762146681550966 18.546506095678833 20.422902949551965 9 28.134348622235013 15.59440121240534 19.712726774802206 36.55852339055744 10-11 25.21937713371967 27.481497125643163 24.96826255166764 22.330863188969523 12-13 27.452138535412494 25.23864008225047 26.860557140171604 20.448664242165435 14-15 23.751215538469037 24.834234204962414 27.777287823263606 23.637262433304944 16-17 20.580603835415513 28.657929488325028 28.097099185618234 22.664367490641226 18-19 20.480111585851247 24.494115517349417 34.45317594962855 20.572596947170783 20-21 21.548973145593077 23.104398218525386 35.37396809777223 19.972660538109306 22-23 21.132150789432764 23.456701301293403 35.335558242569256 20.075589666704573 24-25 20.569463816988065 23.696327739342134 35.47828972867092 20.255918714998874 26-27 20.2946766957777 23.944193149352834 35.28415169920094 20.47697845566853 28-29 20.293447333119815 23.957941691151554 35.40596178036474 20.342649195363887 30-31 20.474217209723854 23.57033363233487 35.41160675225151 20.543842405689773 32-33 20.21115001096597 23.71689466984312 35.21886100141919 20.853094317771713 34-35 20.611218514796565 23.951150792453532 35.02751365238804 20.410117040361868 36-37 20.921472675981807 23.550538380576292 34.66395745406736 20.86403148937454 38-39 20.72026281678602 23.67149758454106 34.72783585787377 20.88040374079915 40-41 20.957300027734036 23.628586712959688 34.557537681736264 20.85657557757001 42-43 20.994909178680054 23.74316359674011 34.06974436812661 21.19218285645323 44-45 20.962851304260038 24.06107086369296 33.71081968963264 21.26525814241436 46-47 21.484942971162404 24.575837504075977 32.46239949312857 21.476820031633046 48-49 21.851000870322018 24.874847693646647 31.48395706411372 21.79019437191761 50-51 21.81606770479761 25.461826697348737 30.978681529601122 21.743424068252526 52-53 22.114586826680842 25.45334705723307 30.604683222764656 21.827382893321435 54-55 21.63136286521274 25.545106630863884 30.74679898532999 22.076731518593387 56-57 21.74932201993383 25.66994410218265 30.61592037607151 21.96481350181201 58-59 21.88066799188161 25.002001747612702 30.885283035508103 22.232047224997594 60-61 21.825562140339844 25.094603893700153 31.246496957937964 21.83333700802204 62-63 21.878067856637244 25.174817060009886 31.278618823381972 21.668496259970897 64-65 21.721330498797798 25.083898847928403 31.21942275735943 21.97534789591437 66-67 21.766536621820148 25.544877076288593 30.919032577742605 21.769553724148654 68-69 22.032208802532516 25.080301525264808 31.187278938720187 21.700210733482486 70-71 21.834638816362055 25.199419785320572 31.01514360313316 21.95079779518422 72-73 21.9616179948431 25.407016819106985 30.589214141325062 22.042151044724854 74-75 21.836989038686035 25.97979943325156 30.271839658001433 21.911371870060968 76-77 21.900584286352533 25.594179185016273 30.41074112111032 22.094495407520874 78-79 21.666003288652988 25.738293312105238 30.498485061229985 22.097218338011793 80-81 21.62672119086797 25.904604308866297 30.348543326548754 22.120131173716985 82-83 21.703772984991147 25.791579538571153 30.175873040923324 22.32877443551438 84-85 21.706285429153297 25.643251272113304 29.917551972428356 22.732911326305043 86-87 21.603280735426882 25.545686840156982 30.07781767039006 22.77321475402607 88-89 21.517641843765883 25.661032447624503 29.96421269080183 22.857113017807784 90-91 21.584855127373494 25.762947095294848 30.028882852126525 22.623314925205133 92-93 21.587110025471276 25.407917563576653 30.15114504702613 22.85382736392594 94-95 21.49402785723486 25.34049858949975 30.32720588661922 22.838267666646168 96-97 21.75815632053194 25.174092404453702 30.119929678388868 22.94782159662549 98-99 21.475634690945718 25.34110504341126 29.92684721232643 23.256413053316592 100-101 21.359203021740516 25.264141200225122 30.24931680117899 23.12733897685537 102-103 21.62115118165425 24.988801960643244 30.22205770065378 23.16798915704873 104-105 21.49559389062823 24.839920256034755 30.195484115029974 23.469001738307043 106-107 21.476985918271435 25.107484145725874 29.977197695400665 23.438332240602026 108-109 21.56636348656345 25.04693914968773 29.768356166609035 23.618341197139785 110-111 21.425620649880823 25.354101731576616 29.77982217745585 23.44045544108671 112-113 21.30530996557034 25.467156054432976 29.691780861658216 23.535753118338466 114-115 21.114085688723026 25.383436205754123 29.52502300559213 23.977455099930722 116-117 20.886219901363503 25.349927473165074 29.931418624891208 23.832434000580214 118-119 20.89428922538488 25.557204641215563 29.602196442848737 23.946309690550816 120-121 20.814104208030802 25.82874290548264 29.46259792493708 23.894554961549485 122-123 20.853166913063355 25.75862717202357 29.241407640695606 24.146798274217467 124-125 20.78913563399257 25.64470329665229 29.31698526143802 24.249175807917116 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 558.0 1 399.0 2 620.0 3 933.5 4 519.0 5 271.0 6 830.5 7 2273.0 8 3938.0 9 4189.0 10 2699.5 11 1245.0 12 883.0 13 933.5 14 1013.0 15 1089.0 16 1152.0 17 1169.0 18 1189.5 19 1285.0 20 1397.5 21 1508.5 22 1595.5 23 1761.5 24 2036.0 25 2281.5 26 2591.5 27 3024.0 28 3464.5 29 4013.0 30 4605.5 31 5347.5 32 6384.0 33 6984.0 34 7399.0 35 7863.5 36 8490.5 37 9428.5 38 10061.0 39 10275.5 40 10545.5 41 11079.0 42 11682.0 43 12328.0 44 13280.5 45 14636.5 46 15102.5 47 15260.5 48 15516.5 49 15373.5 50 15510.5 51 15261.5 52 14480.5 53 14519.5 54 14782.0 55 14119.5 56 12817.5 57 11387.0 58 10571.0 59 9554.0 60 8077.5 61 6904.5 62 5664.5 63 4164.0 64 2993.5 65 2240.0 66 1576.5 67 1196.0 68 853.0 69 506.0 70 362.0 71 278.5 72 222.5 73 212.0 74 163.0 75 98.5 76 65.5 77 32.5 78 16.0 79 8.0 80 4.5 81 3.5 82 2.0 83 1.0 84 0.5 85 1.5 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01856669737907858 2 0.004409590627531163 3 0.0 4 0.14412398840509746 5 0.045024241144265564 6 0.012300437013639559 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.3208371723848226E-4 30-31 1.1604185861924113E-4 32-33 1.1604185861924113E-4 34-35 4.6416743447696453E-4 36-37 8.122930103346879E-4 38-39 0.0019727115965270992 40-41 1.1604185861924113E-4 42-43 0.0012764604448116524 44-45 5.802092930962057E-4 46-47 1.1604185861924113E-4 48-49 9.283348689539291E-4 50-51 0.0019727115965270992 52-53 1.1604185861924113E-4 54-55 0.0 56-57 8.122930103346879E-4 58-59 0.0012764604448116524 60-61 8.122930103346879E-4 62-63 0.0 64-65 6.962511517154468E-4 66-67 4.6416743447696453E-4 68-69 6.962511517154468E-4 70-71 9.283348689539291E-4 72-73 0.0 74-75 0.0 76-77 0.002668962748242546 78-79 8.122930103346879E-4 80-81 0.0 82-83 0.0 84-85 3.481255758577234E-4 86-87 0.0 88-89 0.0 90-91 1.1604185861924113E-4 92-93 3.481255758577234E-4 94-95 8.122930103346879E-4 96-97 3.481255758577234E-4 98-99 0.0 100-101 3.481255758577234E-4 102-103 0.0 104-105 0.0 106-107 3.481255758577234E-4 108-109 4.6416743447696453E-4 110-111 0.0 112-113 1.1604185861924113E-4 114-115 0.0012764604448116524 116-117 9.283348689539291E-4 118-119 1.1604185861924113E-4 120-121 1.1604185861924113E-4 122-123 4.6416743447696453E-4 124-125 5.802092930962057E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 430879.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.29509955494972 #Duplication Level Percentage of deduplicated Percentage of total 1 77.9614655549683 45.4477139597666 2 12.588546176067755 14.67701105171903 3 4.169714759399783 7.292218110448606 4 1.814016090322863 4.229929943186079 5 1.0004239441788 2.915990671152931 6 0.5627686233760743 1.9683991755666153 7 0.3896749041025048 1.5901296130104703 8 0.2652614072009801 1.2370752112693757 9 0.1897385902399084 0.9954747006706144 >10 0.9843677743677514 9.916599156384237 >50 0.042980618197137554 1.6717649218469108 >100 0.027459839396063418 2.9157287722909904 >500 0.0011939060606984096 0.47791225898467854 >1k 0.0015918747475978793 1.7446117415363023 >5k 7.959373737989396E-4 2.9194407121665997 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7245 1.681446531392804 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5339 1.2390949663362567 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3660 0.8494264050928451 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1482 0.3439480689474307 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1263 0.2931217348722031 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1115 0.25877334472090774 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 909 0.21096409896978036 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 602 0.1397143977775663 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 549 0.12741396076392675 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.6416743447696453E-4 0.0 0.0 0.0 0.0 8 4.6416743447696453E-4 0.0 0.0 0.0 0.0 9 4.6416743447696453E-4 0.0 0.0 0.0 0.0 10-11 4.6416743447696453E-4 0.0 0.0 0.0 0.0 12-13 4.6416743447696453E-4 0.0 0.0 0.0 0.0 14-15 6.962511517154468E-4 0.0 0.0 0.0 0.0 16-17 6.962511517154468E-4 0.0 0.0 1.1604185861924113E-4 0.0 18-19 6.962511517154468E-4 0.0 0.0 4.6416743447696453E-4 0.0 20-21 6.962511517154468E-4 0.0 0.0 5.802092930962057E-4 0.0 22-23 6.962511517154468E-4 0.0 0.0 0.0010443767275731702 0.0 24-25 6.962511517154468E-4 0.0 0.0 0.0011604185861924113 0.0 26-27 6.962511517154468E-4 0.0 0.0 0.0029010464654810283 0.0 28-29 6.962511517154468E-4 0.0 0.0 0.004525632486150405 0.0 30-31 6.962511517154468E-4 0.0 0.0 0.00916730683092005 0.0 32-33 6.962511517154468E-4 0.0 0.0 0.016361902065313 0.0 34-35 6.962511517154468E-4 0.0 0.0 0.027617962351379387 0.0 36-37 6.962511517154468E-4 0.0 0.0 0.04664882716493493 0.0 38-39 6.962511517154468E-4 0.0 0.0 0.081809510326565 0.0 40-41 6.962511517154468E-4 0.0 0.0 0.12486103987430346 0.0 42-43 6.962511517154468E-4 0.0 0.0 0.1644313136634647 0.0 44-45 6.962511517154468E-4 0.0 0.0 0.20933951294911102 0.0 46-47 8.122930103346879E-4 0.0 0.0 0.26167439118638874 0.0 48-49 9.283348689539291E-4 0.0 0.0 0.3213199065166787 0.0 50-51 9.283348689539291E-4 0.0 0.0 0.3963989890433277 0.0 52-53 9.283348689539291E-4 0.0 0.0 0.468228899528638 0.0 54-55 9.283348689539291E-4 0.0 0.0 0.5392465170036136 0.0 56-57 9.283348689539291E-4 0.0 0.0 0.6189672738750323 0.0 58-59 9.283348689539291E-4 0.0 0.0 0.7111045096187096 0.0 60-61 9.283348689539291E-4 0.0 0.0 0.8225046938931811 0.0 62-63 9.283348689539291E-4 0.0 0.0 0.9300754968332177 0.0 64-65 9.283348689539291E-4 0.0 0.0 1.0256939883354723 0.0 66-67 9.283348689539291E-4 0.0 0.0 1.149626693340822 0.0 68-69 9.283348689539291E-4 0.0 0.0 1.3001329839699776 0.0 70-71 9.283348689539291E-4 0.0 0.0 1.4694380556954505 0.0 72-73 9.283348689539291E-4 0.0 0.0 1.6373506251174923 0.0 74-75 9.283348689539291E-4 0.0 0.0 1.786812539019075 0.0 76-77 9.283348689539291E-4 0.0 0.0 1.9536807317135438 0.0 78-79 9.283348689539291E-4 0.0 0.0 2.1515321006593497 0.0 80-81 0.0013925023034308936 0.0 0.0 2.38083081329097 0.0 82-83 0.0013925023034308936 0.0 0.0 2.636703111546397 0.0 84-85 0.0013925023034308936 0.0 0.0 2.878186219333038 0.0 86-87 0.0013925023034308936 0.0 0.0 3.1311574711229833 0.0 88-89 0.0013925023034308936 0.0 0.0 3.430545466360625 0.0 90-91 0.0016245860206693758 0.0 0.0 3.763353516880609 0.0 92-93 0.0016245860206693758 0.0 0.0 4.122387027448541 0.0 94-95 0.0016245860206693758 0.0 0.0 4.483277207754381 0.0 96-97 0.0016245860206693758 0.0 0.0 4.8200306814674185 0.0 98-99 0.0016245860206693758 0.0 0.0 5.191248587190371 0.0 100-101 0.001740627879288617 0.0 0.0 5.661450198315537 0.0 102-103 0.0019727115965270992 0.0 0.0 6.136757651219948 0.0 104-105 0.002785004606861787 0.0 0.0 6.615778443600176 0.0 106-107 0.0031331301827195106 0.0 0.0 7.056737506353292 0.0 108-109 0.0032491720413387517 0.0 0.0 7.5314647499646075 0.0 110-111 0.003365213899957993 0.0 0.0 8.073960439009559 0.0 112-113 0.003481255758577234 0.0 0.0 8.66264078778497 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1950 0.0 83.5152 1 ATAACGC 85 0.0 77.171974 3 TAACGCA 95 0.0 75.32576 4 TATAACG 90 0.0 72.89313 2 GTATCAA 3975 0.0 59.4286 1 GTATAAC 180 0.0 49.71143 1 GTGGTAT 570 0.0 49.188152 1 TGGTATC 605 0.0 46.331768 2 TATCAAC 5295 0.0 44.603157 2 ATCAACG 5315 0.0 44.31796 3 TCAACGC 5380 0.0 43.782513 4 CAACGCA 5490 0.0 42.933987 5 AACGCAG 5720 0.0 41.18849 6 CCTATAC 90 5.0696653E-6 39.75526 3 ACGCAGA 6400 0.0 36.719257 7 CGCAGAG 6520 0.0 35.86094 8 GCAGAGT 6785 0.0 34.197277 9 TAAGGTG 265 0.0 33.691765 5 GTACATG 3190 0.0 33.099396 1 TACATGG 3320 0.0 31.616295 2 >>END_MODULE