FastQCFastQC Report
Fri 27 May 2016
SRR940041_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940041_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971338
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17200.052.6886941
GGTATCA10250.049.4421541
TCAACGC19000.045.7954444
ATCAACG19150.045.7479483
CAACGCA20050.043.377045
AACGCAG20850.041.7126926
TATCAAC21700.041.2214742
ACGCAGA25700.033.6090557
CGCAGAG26100.032.8657388
GTACATG42100.032.4306531
TACATGG43100.030.439492
GTCTACG800.005308624429.8107091
ACATGGG44400.027.9192283
GCAGAGT32100.026.5315639
CATGGGG29750.026.0423034
CGCGGAA1351.718945E-924.23392382-83
GGACCGA1802.1571339E-523.1657836
ATAGGAC3404.1836756E-1122.7870163
CCTACGC1505.809852E-921.81053296-97
CAATTCG1657.403287E-1021.62860978-79
Produced by FastQC (version 0.11.5)