FastQCFastQC Report
Fri 27 May 2016
SRR940041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940041_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971338
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32810.33778149315686196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24090.2480084172553735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14550.14979337779434143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17550.069.2527541
GTATCAA32900.056.8614231
CGCGATA255.3344056E-447.598396104-105
ATCAACG41100.045.0819363
TCAACGC41250.044.9184
CAACGCA42050.043.8674855
TATCAAC42700.043.3926852
AACGCAG43700.042.204646
GTGGTAT8050.041.445381
TGGTATC8050.038.4849972
ACGCAGA50650.036.2960177
CGCAGAG50800.035.9546138
GTACATG39100.034.131491
TACATGG41050.032.219872
TATACCG750.003908865631.735535
GCAGAGT59600.030.4462289
ACATGGG42850.029.8931473
TATCACG1055.8600557E-428.370352
TAACACG850.007164017828.0365814
CAGAGTA56450.026.1377210-11
Produced by FastQC (version 0.11.5)