FastQCFastQC Report
Fri 27 May 2016
SRR940040_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940040_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542228
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13300.056.5310171
GTATCAA23250.048.7639161
ATCAACG27300.039.520083
CAACGCA29000.037.1840445
TCAACGC29200.036.9497684
TATCAAC31100.034.911292
AACGCAG31100.034.864796
GTACATG64800.034.3480221
TACATGG68450.031.810782
ACATGGG69600.030.4033183
ACGCAGA37250.028.468847
CGCAGAG38250.027.2572928
CATGGGG45300.025.7913384
CATGGGA34000.022.9672164
GAGTACA48200.022.0364131
GCAGAGT46550.021.8781919
ATGGGGA26450.020.9475565
AGAGTAC41250.020.48385610-11
AGTACAT49700.020.1654452
CAGAGTA43800.019.69886610-11
Produced by FastQC (version 0.11.5)