FastQCFastQC Report
Fri 27 May 2016
SRR940040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940040_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542228
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33440.21682915885329535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24730.16035242519264337No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22250.065.6531141
GTATCAA43350.057.904491
ATCAACG53050.046.9690063
CAACGCA54300.046.0400285
TCAACGC54500.045.7178884
AACGCAG56250.044.5310136
TATCAAC56850.044.2488982
ACGCAGA66900.036.9059877
GTGGTAT11700.036.181931
CGCAGAG68650.035.7052048
TGGTATC11700.033.626262
GTACATG66150.033.5299071
CTAGCGT750.003876250231.7910654
TACATGG69550.031.5406912
GCAGAGT77500.031.0137659
ACATGGG69150.030.7748933
TAACGCA1107.6705456E-427.0946624
GAGTACT43000.025.73471312-13
GTATAGG5200.025.2254311
CAGAGTA76500.025.0031310-11
Produced by FastQC (version 0.11.5)