FastQCFastQC Report
Fri 27 May 2016
SRR940039_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940039_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590779
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25600.044.5116581
GGTATCA15800.040.402391
ATCAACG27900.039.10323
TCAACGC28900.037.9612164
CAACGCA28900.037.5273065
TATCAAC31550.035.9303932
AACGCAG31000.034.7929046
GTACATG59200.031.6437761
TACATGG58700.031.1022032
ACGCAGA37450.028.8005357
CGCAGAG38800.027.7984548
ACATGGG62450.027.4932123
GCAGAGT44650.022.946519
GAGTACA45200.022.4383471
CATGGGG44500.021.9737424
ATGGGGA23950.021.3976845
TACCGTG2055.870068E-520.3478247
AGTACAT45550.020.1715892
AGAGTAC40300.020.0056110-11
TAAGGTA5200.019.492424
Produced by FastQC (version 0.11.5)