##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940039_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1590779 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.36102626449054 33.0 31.0 34.0 30.0 34.0 2 31.449988339046467 34.0 31.0 34.0 28.0 34.0 3 31.707997151081326 34.0 31.0 34.0 30.0 34.0 4 34.95136030837722 37.0 35.0 37.0 33.0 37.0 5 35.024643900881266 37.0 35.0 37.0 33.0 37.0 6 35.030356196555275 37.0 37.0 37.0 33.0 37.0 7 35.00366990009297 37.0 37.0 37.0 33.0 37.0 8 34.94422669648015 37.0 37.0 37.0 32.0 37.0 9 36.62002012850308 39.0 38.0 39.0 33.0 39.0 10-11 36.61363269190755 39.0 38.0 39.0 33.0 39.0 12-13 36.5630876444811 39.0 38.0 39.0 33.0 39.0 14-15 38.02624720341417 41.0 39.0 41.0 33.0 41.0 16-17 38.04891377117752 41.0 39.0 41.0 33.0 41.0 18-19 38.05391666598566 41.0 39.0 41.0 33.0 41.0 20-21 38.02752739381146 41.0 39.0 41.0 33.0 41.0 22-23 38.02477654029881 41.0 39.0 41.0 33.0 41.0 24-25 38.05237559711311 41.0 39.0 41.0 33.0 41.0 26-27 38.05634975065675 41.0 39.0 41.0 33.0 41.0 28-29 38.0012569313525 41.0 39.0 41.0 33.0 41.0 30-31 37.939564200935514 41.0 39.0 41.0 33.0 41.0 32-33 37.870181841726605 41.0 39.0 41.0 32.0 41.0 34-35 37.78278629526791 41.0 38.5 41.0 32.0 41.0 36-37 37.661127032730505 41.0 38.0 41.0 32.0 41.0 38-39 37.56349782087895 40.5 38.0 41.0 31.0 41.0 40-41 37.45398229420931 40.0 38.0 41.0 31.0 41.0 42-43 37.305561929092605 40.0 38.0 41.0 31.0 41.0 44-45 37.137962281372836 40.0 38.0 41.0 30.0 41.0 46-47 36.96042347805698 40.0 37.5 41.0 30.0 41.0 48-49 36.79807628840964 40.0 37.0 41.0 30.0 41.0 50-51 36.615137300655846 40.0 37.0 41.0 30.0 41.0 52-53 36.75395136596599 40.0 37.0 41.0 30.0 41.0 54-55 36.5612602379086 40.0 36.0 41.0 30.0 41.0 56-57 36.4245143417156 40.0 36.0 41.0 30.0 41.0 58-59 36.21580213216292 39.5 35.5 41.0 30.0 41.0 60-61 35.96148427908591 39.0 35.0 41.0 30.0 41.0 62-63 35.66795450530841 39.0 35.0 41.0 29.0 41.0 64-65 35.34287635177482 38.0 35.0 41.0 29.0 41.0 66-67 35.013617227785886 37.0 35.0 40.0 29.0 41.0 68-69 34.655961010297474 37.0 35.0 40.0 28.0 41.0 70-71 34.29068588408572 36.0 34.5 39.0 28.0 41.0 72-73 33.91881776161239 36.0 34.0 39.0 28.0 41.0 74-75 33.55140531777198 35.0 34.0 38.0 27.0 40.0 76-77 33.1862141127083 35.0 34.0 37.0 27.0 39.0 78-79 32.85419722035556 35.0 34.0 37.0 26.0 39.0 80-81 32.542676575438826 35.0 34.0 36.0 26.0 38.5 82-83 32.2442800036963 35.0 33.5 36.0 26.0 37.0 84-85 32.016487519636605 35.0 33.0 35.5 25.5 37.0 86-87 31.80660795748498 35.0 33.0 35.0 25.0 36.5 88-89 31.63879489231377 35.0 33.0 35.0 25.0 36.0 90-91 31.48835004736673 35.0 33.0 35.0 24.0 36.0 92-93 31.341478608907963 35.0 33.0 35.0 24.0 36.0 94-95 31.232040088535243 35.0 33.0 35.0 24.0 35.0 96-97 31.12417343955383 35.0 33.0 35.0 23.0 35.0 98-99 31.052775401234236 35.0 33.0 35.0 23.0 35.0 100-101 30.94693857537722 35.0 33.0 35.0 20.5 35.0 102-103 30.230265800592036 34.0 31.0 34.5 21.5 35.0 104-105 31.191065509413942 35.0 33.0 35.0 24.0 35.0 106-107 31.32001522524499 35.0 33.0 35.0 24.0 35.0 108-109 31.30338909427394 35.0 34.0 35.0 24.0 35.0 110-111 31.251466734222667 35.0 33.0 35.0 23.5 35.0 112-113 31.19518770363451 35.0 33.0 35.0 23.0 35.0 114-115 31.12819379687562 35.0 33.0 35.0 23.0 35.0 116-117 31.045805231273484 35.0 33.0 35.0 20.0 35.0 118-119 30.955412096840604 35.0 33.0 35.0 19.0 35.0 120-121 30.84925530196212 35.0 33.0 35.0 18.5 35.0 122-123 30.701166221077848 35.0 33.0 35.0 8.5 35.0 124-125 29.385554184459313 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1967400283802725 1101 2 0.44262953990528686 1101 3 0.4491336612002286 1101 4 0.34733020213806753 1101 5 0.32190933162805635 1101 6 0.42930266135427075 1101 7 0.5226713362760975 1101 8 0.6003560408172817 1101 9 0.6257518239197921 1101 10-11 0.40894670483578466 1101 12-13 0.5177539225730854 1101 14-15 0.6008035207517537 1101 16-17 0.5969555195826999 1101 18-19 0.6243330647384582 1101 20-21 0.4513558042171013 1101 22-23 0.5198393051194614 1101 24-25 0.4358883444377142 1101 26-27 0.5725500977153857 1101 28-29 0.5255356531014641 1101 30-31 0.6193624866083596 1101 32-33 0.5785700363381494 1101 34-35 0.5726033126702461 1101 36-37 0.5948043087052994 1101 38-39 0.6678547581330747 1101 40-41 0.5884667488746871 1101 42-43 0.6258739259164017 1101 44-45 0.5794343400447488 1101 46-47 0.6016984253655622 1101 48-49 0.5787354498254871 1101 50-51 0.5503760438602185 1101 52-53 0.6464636815233362 1101 54-55 0.7554722253706032 1101 56-57 0.6497972327135884 1101 58-59 0.651525856706769 1101 60-61 0.5857330908806375 1101 62-63 0.5890292036143165 1101 64-65 0.6025151399618238 1101 66-67 0.5255917322726873 1101 68-69 0.5329440047389369 1101 70-71 0.5530671693189291 1101 72-73 0.4847320820985601 1101 74-75 0.42817578943762413 1101 76-77 0.39789348010209125 1101 78-79 0.44365083365609337 1101 80-81 0.3820988472639968 1101 82-83 0.41795405869890345 1101 84-85 0.43212631025143367 1101 86-87 0.3103423908165368 1101 88-89 0.3121793440891949 1101 90-91 0.3320190015638751 1101 92-93 0.3771184989554399 1101 94-95 0.2990809342479466 1101 96-97 0.4293222040478959 1101 98-99 0.363832342614252 1101 100-101 0.37998918518333724 1101 102-103 0.34815522947453204 1101 104-105 0.4979748672275619 1101 106-107 0.4104309782433617 1101 108-109 0.4148817445775457 1101 110-111 0.5031139952417902 1101 112-113 0.43571907814133937 1101 114-115 0.4776801506119739 1101 116-117 0.5395549221179081 1101 118-119 0.5462909775373355 1101 120-121 0.5350619227589988 1101 122-123 0.3776981769818377 1101 124-125 0.2506947536280002 1102 1 -0.13260982610634287 1102 2 -0.07728918675751828 1102 3 0.06531410021869632 1102 4 -0.17498495904104772 1102 5 -0.08250304407143716 1102 6 0.023781946803978826 1102 7 0.04133370331479824 1102 8 0.073983753794586 1102 9 0.0342782565295181 1102 10-11 0.015086805165282158 1102 12-13 -0.017978558586968063 1102 14-15 0.11497148290300885 1102 16-17 -0.018125059680002664 1102 18-19 0.10813375367704481 1102 20-21 -0.05707568675423147 1102 22-23 -0.11757593724093596 1102 24-25 -0.011648587472002703 1102 26-27 0.019360128920880015 1102 28-29 0.016566950894791432 1102 30-31 0.04971713808242839 1102 32-33 0.018261503873276297 1102 34-35 -0.06378232529323213 1102 36-37 -3.7353304523435327E-4 1102 38-39 0.06231279739321849 1102 40-41 -0.03768110861546603 1102 42-43 -0.12291883264573045 1102 44-45 -0.10172794523124651 1102 46-47 -0.005975058735764094 1102 48-49 0.009373060813807399 1102 50-51 0.07517642779363598 1102 52-53 0.023167828212642405 1102 54-55 0.16531727838796684 1102 56-57 0.045293839985404816 1102 58-59 0.025529290497992463 1102 60-61 0.03675069130158448 1102 62-63 -0.00369127526558799 1102 64-65 -0.06907824430906828 1102 66-67 -0.04378228521217409 1102 68-69 -0.02017970598438268 1102 70-71 -0.04986541048934612 1102 72-73 -0.13943457427206596 1102 74-75 -0.06230848559611957 1102 76-77 0.025886509801452462 1102 78-79 -0.1321274443075353 1102 80-81 -0.14516858993437864 1102 82-83 -0.12407778442136674 1102 84-85 -0.1110798875309662 1102 86-87 -0.14998077414377065 1102 88-89 -0.05584693335308799 1102 90-91 -0.08568968531743693 1102 92-93 -0.10510762203543322 1102 94-95 -0.0914218494389516 1102 96-97 -0.13189516627464215 1102 98-99 -0.1432005821962825 1102 100-101 -0.11783362547193121 1102 102-103 -0.11145617602268132 1102 104-105 -0.1645543644525631 1102 106-107 -0.11844276294413447 1102 108-109 -0.16195981190328013 1102 110-111 -0.06366021057588611 1102 112-113 -0.058698043428776714 1102 114-115 -0.06659804704251471 1102 116-117 -0.01783353114738162 1102 118-119 -0.06447471139276928 1102 120-121 -0.017062466049662106 1102 122-123 0.10142685175500787 1102 124-125 -0.0995779946332398 1103 1 -0.011424286218144175 1103 2 0.21850040215974076 1103 3 0.17610903910894038 1103 4 0.1606755242575062 1103 5 0.23076312326441695 1103 6 0.2057763743568657 1103 7 0.15766860102966973 1103 8 0.23929474083811186 1103 9 0.1425165086597957 1103 10-11 0.24391168271623087 1103 12-13 0.346097167367283 1103 14-15 0.4346480832158832 1103 16-17 0.4337400150607067 1103 18-19 0.42324115924031247 1103 20-21 0.3002135190131412 1103 22-23 0.3977387577046585 1103 24-25 0.4618639258146473 1103 26-27 0.4580437883730255 1103 28-29 0.42111283883139805 1103 30-31 0.41761412196894554 1103 32-33 0.46974731320235463 1103 34-35 0.4485801733383781 1103 36-37 0.526917685583264 1103 38-39 0.48016616928873646 1103 40-41 0.45338602559999686 1103 42-43 0.49750570448449594 1103 44-45 0.45167749796898704 1103 46-47 0.49098905230354006 1103 48-49 0.49625662209483323 1103 50-51 0.4601724903547719 1103 52-53 0.48338097961158866 1103 54-55 0.5307776409148488 1103 56-57 0.5176978671201837 1103 58-59 0.5550279468249144 1103 60-61 0.5183760943152294 1103 62-63 0.5265690900863689 1103 64-65 0.47231253678699403 1103 66-67 0.49122278225399185 1103 68-69 0.46274734722224764 1103 70-71 0.40829709449866414 1103 72-73 0.432797123699892 1103 74-75 0.40436489060762426 1103 76-77 0.4103479281975879 1103 78-79 0.3575211525163837 1103 80-81 0.3464646173302839 1103 82-83 0.4148742565138761 1103 84-85 0.41298263122925505 1103 86-87 0.4368957244666447 1103 88-89 0.4043086572804029 1103 90-91 0.36512693597157053 1103 92-93 0.4087684069073916 1103 94-95 0.3216543320575589 1103 96-97 0.29635657555649786 1103 98-99 0.2646237974363892 1103 100-101 0.29971817038864046 1103 102-103 0.3545370650015478 1103 104-105 0.49235804783789305 1103 106-107 0.39543926926870654 1103 108-109 0.3820265006410821 1103 110-111 0.44820754797965634 1103 112-113 0.40036495206171097 1103 114-115 0.32879356038599994 1103 116-117 0.4488140663086426 1103 118-119 0.4604706411934032 1103 120-121 0.5010126737029346 1103 122-123 0.48154896532981084 1103 124-125 0.5174253848546435 1104 1 -0.02634919927164958 1104 2 0.18702224114283084 1104 3 0.19306701771468937 1104 4 0.21823786924802135 1104 5 0.09352202145583988 1104 6 0.3037223663469675 1104 7 0.2980604838272072 1104 8 0.3989066229767886 1104 9 0.479296405782911 1104 10-11 0.4047452866543537 1104 12-13 0.41330869089578215 1104 14-15 0.4652118353513188 1104 16-17 0.471193012185239 1104 18-19 0.42790544458482316 1104 20-21 0.35490733557603704 1104 22-23 0.3195826822741594 1104 24-25 0.3613741311448564 1104 26-27 0.3045996953226222 1104 28-29 0.2846664915254493 1104 30-31 0.30670745473754124 1104 32-33 0.38216772009783284 1104 34-35 0.4377729239537089 1104 36-37 0.349619074374111 1104 38-39 0.34994220264606923 1104 40-41 0.3008245747818279 1104 42-43 0.28387628244926333 1104 44-45 0.3764616024083125 1104 46-47 0.38545541965778085 1104 48-49 0.32011831716323513 1104 50-51 0.26291148144598964 1104 52-53 0.2819428916344364 1104 54-55 0.33587171725834253 1104 56-57 0.256597423328337 1104 58-59 0.19317839838194573 1104 60-61 0.2685874013598948 1104 62-63 0.3308233503469893 1104 64-65 0.3712804736490085 1104 66-67 0.3548945598215525 1104 68-69 0.26018719060090234 1104 70-71 0.2664819481602976 1104 72-73 0.29899482050016246 1104 74-75 0.30856055904851587 1104 76-77 0.2315172232603473 1104 78-79 0.2852956751930691 1104 80-81 0.27289611666906666 1104 82-83 0.2796115110282713 1104 84-85 0.28135046079059123 1104 86-87 0.299385312064949 1104 88-89 0.4972792860718087 1104 90-91 0.44237977967494757 1104 92-93 0.3512304163479705 1104 94-95 0.26510734330059904 1104 96-97 0.36856593424667494 1104 98-99 0.4068647345290124 1104 100-101 0.4658797473321705 1104 102-103 0.3200349542841856 1104 104-105 0.37385009205706865 1104 106-107 0.3079342689628035 1104 108-109 0.31787610555808854 1104 110-111 0.3322582193148449 1104 112-113 0.32563567450590725 1104 114-115 0.3552455315654157 1104 116-117 0.3590619873579328 1104 118-119 0.3004002604171703 1104 120-121 0.3253519273984544 1104 122-123 0.37954778408107614 1104 124-125 0.4116267948232526 1105 1 0.19336362981838207 1105 2 0.30587710756709185 1105 3 0.35721219337881394 1105 4 0.21796017737528928 1105 5 0.20696349755054655 1105 6 0.19837467050328428 1105 7 0.23424579351540586 1105 8 0.3082331355511485 1105 9 0.29302188700874865 1105 10-11 0.29997127385582445 1105 12-13 0.3542520472435413 1105 14-15 0.4018500527067346 1105 16-17 0.38573459642881147 1105 18-19 0.3091882803906927 1105 20-21 0.2745713512841519 1105 22-23 0.3209619919879856 1105 24-25 0.28746028607771024 1105 26-27 0.30514295578205264 1105 28-29 0.38728736297469624 1105 30-31 0.42693133751954804 1105 32-33 0.45782091360011634 1105 34-35 0.425888443772763 1105 36-37 0.46172744648671227 1105 38-39 0.49179608639794026 1105 40-41 0.41756884572858155 1105 42-43 0.44844257074741023 1105 44-45 0.3796397332812518 1105 46-47 0.3953857633735183 1105 48-49 0.3997211857614573 1105 50-51 0.39499491161311795 1105 52-53 0.40060447170576907 1105 54-55 0.5684436929788319 1105 56-57 0.4931950162384524 1105 58-59 0.39039338221451914 1105 60-61 0.4074228156285926 1105 62-63 0.4071334887079985 1105 64-65 0.4014070682042359 1105 66-67 0.41780457850514097 1105 68-69 0.3736428486528638 1105 70-71 0.4251572344071022 1105 72-73 0.34759638801818227 1105 74-75 0.3078370504871941 1105 76-77 0.332642813184016 1105 78-79 0.4094324057303851 1105 80-81 0.44339206428914935 1105 82-83 0.4000398884842653 1105 84-85 0.3478416524226873 1105 86-87 0.3351592992444594 1105 88-89 0.3176895014728629 1105 90-91 0.28906893313104476 1105 92-93 0.34817145966424334 1105 94-95 0.46189480974425834 1105 96-97 0.30652353110944475 1105 98-99 0.24452341233141084 1105 100-101 0.25709777616820517 1105 102-103 0.2881237205270999 1105 104-105 0.34479263408598726 1105 106-107 0.3903755876835362 1105 108-109 0.39297483027989344 1105 110-111 0.40473511187416733 1105 112-113 0.4307532566560397 1105 114-115 0.2747020789633474 1105 116-117 0.3924014814554937 1105 118-119 0.36877399250444043 1105 120-121 0.46865625939166833 1105 122-123 0.4487504744441182 1105 124-125 0.35716785021706343 1106 1 0.28991793862799753 1106 2 0.45992018175040883 1106 3 0.30384380546073686 1106 4 0.34484232510192925 1106 5 0.34771849574187996 1106 6 0.38266514994009526 1106 7 0.41738933238791276 1106 8 0.3320323696487648 1106 9 0.455891794905682 1106 10-11 0.520232774565379 1106 12-13 0.4922794746880257 1106 14-15 0.6344468165778423 1106 16-17 0.6322266017574307 1106 18-19 0.6303655509678023 1106 20-21 0.4994119187197441 1106 22-23 0.5560448082270568 1106 24-25 0.5126147621893438 1106 26-27 0.6054911683286548 1106 28-29 0.6178324162609101 1106 30-31 0.6217305837908995 1106 32-33 0.5944692297974612 1106 34-35 0.5734289997302469 1106 36-37 0.6313055219221866 1106 38-39 0.5972941838157126 1106 40-41 0.5777798775750895 1106 42-43 0.5445905808620566 1106 44-45 0.630194073394776 1106 46-47 0.5448645532338432 1106 48-49 0.5372727502614296 1106 50-51 0.545412601453954 1106 52-53 0.5376460433012937 1106 54-55 0.6738270093848655 1106 56-57 0.633236546095425 1106 58-59 0.5163935856899471 1106 60-61 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-1.471396470368198 2216 106-107 -1.6162564744603607 2216 108-109 -1.5401742378586327 2216 110-111 -1.5412145892428804 2216 112-113 -1.6854610905742575 2216 114-115 -1.6380236741835148 2216 116-117 -1.5799640424275694 2216 118-119 -1.6455698438035782 2216 120-121 -1.7599584425102393 2216 122-123 -1.7227041882572394 2216 124-125 -1.7690407272358364 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32515.0 3 4839.0 4 3015.0 5 2603.0 6 2744.0 7 3158.0 8 3596.0 9 4020.0 10 4389.0 11 4798.0 12 5310.0 13 5179.0 14 5414.0 15 5158.0 16 4950.0 17 4901.0 18 4127.0 19 3826.0 20 3608.0 21 3405.0 22 3531.0 23 4562.0 24 7286.0 25 11667.0 26 12787.0 27 11976.0 28 13550.0 29 15497.0 30 18557.0 31 22956.0 32 29483.0 33 40255.0 34 60649.0 35 108595.0 36 264566.0 37 580073.0 38 272263.0 39 971.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.803604121475736 18.104946311759715 13.650831785652068 24.44061778111248 2 15.513863426965338 18.923560489028475 40.66288259273511 24.899693491271083 3 19.676999496981892 23.28678319919517 27.969001509054326 29.067215794768615 4 13.028916437543225 15.750141438754659 36.22361356981348 34.99732855388864 5 14.68299179090964 37.09878825375747 33.43827337478119 14.7799465805517 6 33.62342135332067 35.030848185173376 17.13015978422935 14.215570677276606 7 30.086134307136692 31.44565652805229 20.034071378846942 18.434137785964076 8 26.32777163913506 35.92018394114287 19.23352823174369 18.518516187978385 9 26.247800553291427 17.18658356387143 19.299877409951694 37.265738472885445 10-11 25.639922591048915 26.51410663038987 26.608289198029933 21.237681580531284 12-13 25.754173397784374 23.371027592499548 27.13213197161957 23.742667038096503 14-15 22.93583547667742 25.022145967862098 24.626473437726922 27.415545117733558 16-17 23.510216632024868 28.140092251670808 25.20292255003463 23.146768566269692 18-19 23.547327044025156 27.186446540880503 26.32235849056604 22.943867924528302 20-21 24.195097647712398 26.442670469361556 26.34182934986767 23.020402533058377 22-23 24.015076738256735 26.13814176274747 26.10913109267336 23.737650406322437 24-25 23.76649411199246 26.30775671898459 26.222449261407903 23.703299907615044 26-27 23.615129647303505 26.671029948206463 26.095347132368378 23.61849327212165 28-29 23.501885520890667 26.75292776743451 26.18068624347722 23.56450046819761 30-31 23.6963489093753 26.405622111240202 26.168360316047295 23.729668663337204 32-33 23.59415293352897 26.396417125276137 26.18804685800646 23.821383083188426 34-35 23.99184986500789 26.58893526759683 25.871303433788167 23.547911433607112 36-37 24.009340494924274 26.46667395901699 25.835948521035917 23.68803702502282 38-39 23.61051823566134 26.49657655029683 26.135528498780687 23.75737671526115 40-41 23.858035236898694 26.292355426778496 26.03636596811259 23.81324336821022 42-43 23.688622061810236 26.28718935589216 25.963920505536652 24.06026807676095 44-45 23.735424338584423 26.461275381179384 26.05863427683187 23.744666003404326 46-47 23.906784909290387 26.642534021999165 25.8362542949925 23.61442677371795 48-49 23.916391466245837 26.30437140346713 26.11493501288111 23.66430211740592 50-51 23.742779508767693 26.53808722680327 26.009077009352378 23.710056255076662 52-53 23.86542459855662 26.4636706394488 25.987710089382883 23.683194672611695 54-55 23.804055545112014 26.297040733375542 26.02653903051938 23.872364690993063 56-57 23.6941169443759 26.62871315918939 26.16632375431653 23.510846142118176 58-59 23.868224657499795 26.235955762617024 25.986130234956086 23.9096893449271 60-61 23.754981488571573 26.333693565919102 26.194818560339588 23.716506385169733 62-63 23.684395838756203 26.409411965902247 26.19488634291657 23.71130585242498 64-65 23.589999629001053 26.29936093858961 26.123953893005158 23.986685539404178 66-67 23.635294125044563 26.62577867540766 25.87491141948275 23.864015780065028 68-69 23.47957512461056 26.296797147877903 26.397787272414575 23.825840455096966 70-71 23.49078848088531 26.226515342052316 26.311839788732392 23.97085638832998 72-73 23.55078090371191 26.29647117405593 26.074783924090667 24.0779639981415 74-75 23.503944655995443 26.587214021084687 25.904969908206354 24.00387141471351 76-77 23.767307394285286 26.43209679787076 25.937382022722677 23.863213785121275 78-79 23.613222720624943 26.376434060565362 26.090124961173633 23.920218257636062 80-81 23.575010382108758 26.45458766602336 26.140216469718354 23.83018548214953 82-83 23.689986739693712 26.478549301114583 26.07599306113133 23.755470898060373 84-85 23.689106316307015 26.383039990745154 25.961542088803775 23.966311604144057 86-87 23.662434740070086 26.352970470412252 26.207249754931393 23.77734503458627 88-89 23.72157072342566 26.397742635268965 26.074857735718552 23.80582890558682 90-91 23.67661049304972 26.435156162068253 26.224072808351785 23.66416053653025 92-93 23.526953785515442 26.292698233432414 26.23503467045305 23.945313310599094 94-95 23.493617192520464 26.40942399422523 26.23814266653085 23.85881614672346 96-97 23.746534197749504 26.289070937061126 26.13313145038499 23.83126341480438 98-99 23.461681037044162 26.327455957410486 25.98471121373645 24.2261517918089 100-101 23.36933029060488 26.2257707447255 26.367773821196845 24.037125143472778 102-103 23.388323947452484 25.91634954210819 26.468938561256394 24.226387949182936 104-105 23.259185948612636 26.178625256304894 26.35999574947293 24.202193045609537 106-107 23.30396298480883 26.264379669816247 26.216746129263907 24.214911216111016 108-109 23.381198614719487 26.20048841952773 26.172037101775864 24.246275863976923 110-111 23.17109553973128 26.320668916129897 26.33497301241438 24.173262531724443 112-113 23.25398829017945 26.268844225055165 26.46078014958112 24.016387335184262 114-115 23.14249940980333 26.482210953735812 26.204386285055403 24.170903351405453 116-117 22.961403341120732 26.287152553369665 26.59099939394092 24.160444711568687 118-119 23.05706747287352 26.46079928165762 26.238235131369127 24.243898114099732 120-121 22.978576647338368 26.430287962756864 26.547898848978946 24.04323654092582 122-123 22.91898315155116 26.449345411277253 26.41313044147997 24.218540995691612 124-125 23.079162346721862 26.44780762670144 26.278803212341344 24.194226814235353 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 238.0 1 222.5 2 195.5 3 164.5 4 131.0 5 106.0 6 98.5 7 106.5 8 100.5 9 97.0 10 109.0 11 118.0 12 133.5 13 136.5 14 149.0 15 177.5 16 214.0 17 248.0 18 274.0 19 354.5 20 488.5 21 707.5 22 1040.5 23 1556.0 24 2208.5 25 3101.0 26 4423.5 27 6181.5 28 7937.0 29 10028.0 30 12598.0 31 15419.0 32 18718.0 33 21804.0 34 24614.0 35 27627.0 36 31521.0 37 35048.5 38 37373.5 39 39128.5 40 41546.0 41 44649.5 42 47435.5 43 50369.0 44 53846.0 45 56967.5 46 58553.0 47 60348.5 48 61700.0 49 61638.5 50 61333.0 51 61470.0 52 63164.5 53 65102.5 54 66421.5 55 63807.5 56 57088.5 57 51370.5 58 47945.0 59 43295.5 60 37005.5 61 30637.5 62 24046.5 63 18887.5 64 14799.0 65 11065.0 66 7978.5 67 6322.5 68 4860.5 69 3294.5 70 2422.5 71 1757.5 72 1282.5 73 1012.5 74 762.0 75 547.5 76 386.5 77 230.5 78 150.5 79 110.0 80 71.5 81 51.0 82 39.0 83 27.5 84 18.0 85 16.5 86 18.0 87 16.0 88 15.0 89 15.0 90 11.5 91 11.0 92 11.5 93 9.0 94 6.0 95 5.5 96 6.0 97 5.0 98 4.0 99 6.0 100 15.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04815250892801577 2 0.18204917213516145 3 0.02382480533122451 4 0.11013472015911702 5 0.021813212268957535 6 0.03595722598802222 7 0.03696302251915571 8 0.0990080960334528 9 0.10956895961035443 10-11 0.04915830545914927 12-13 0.03319128552740513 14-15 0.012321007506385236 16-17 0.028633769995706507 18-19 0.04896971860956173 20-21 0.19694753325257627 22-23 0.10740021084009785 24-25 0.07760348860526824 26-27 0.014646911984631427 28-29 0.006569108593965598 30-31 0.00823495909865544 32-33 0.006349090602780148 34-35 0.008203527957057517 36-37 0.004463222106904856 38-39 0.008297821381851282 40-41 0.005563312062832109 42-43 0.009586498187366064 44-45 0.009837947320149436 46-47 0.016595642763702564 48-49 0.017067109887671388 50-51 0.010498001293705789 52-53 0.012949630338343667 54-55 0.013578253170302097 56-57 0.01486692997581688 58-59 0.016909954179681778 60-61 0.008800719647418026 62-63 0.01841864897638201 64-65 0.030048171367612977 66-67 0.02627643437586239 68-69 0.03297126753621967 70-71 0.02382480533122451 72-73 0.01571557079896076 74-75 0.009177893346593084 76-77 0.010403707868912024 78-79 0.02294473336648271 80-81 0.01901584066674252 82-83 0.019990206056278086 84-85 0.016407055914115034 86-87 0.02564781154390396 88-89 0.026967919491016665 90-91 0.025899260676687335 92-93 0.032688387261838384 94-95 0.025962122959883177 96-97 0.026307865517460314 98-99 0.03322271666900305 100-101 0.020147361764267693 102-103 0.02809944058854184 104-105 0.0243905658799871 106-107 0.03224835127946748 108-109 0.021561763136174163 110-111 0.02046167318024691 112-113 0.02542779355271851 114-115 0.01304392376313743 116-117 0.011000899559272533 118-119 0.028350889721325212 120-121 0.02294473336648271 122-123 0.017381421303650602 124-125 0.0181043375604028 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1590779.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.5225564465729 #Duplication Level Percentage of deduplicated Percentage of total 1 74.49801553051064 40.61822256919931 2 13.75333159948009 14.997335969221758 3 4.9666588351917955 8.123848100778442 4 2.2703439234341825 4.951398188742959 5 1.2346146103754305 3.3657172391979002 6 0.7367468731571796 2.4101593787129043 7 0.5093731338406754 1.9440627809537168 8 0.33529139204166153 1.4624755078913196 9 0.25496699759984764 1.251130726678582 >10 1.306854439506653 13.081332842534701 >50 0.0944578346733138 3.5628692527608625 >100 0.03738345988934418 3.5588645238856844 >500 0.00196137029908156 0.6725829194419523 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.286228319584305E-5 2 0.0 0.0 0.0 0.0 6.286228319584305E-5 3 0.0 0.0 0.0 0.0 6.286228319584305E-5 4 0.0 0.0 0.0 0.0 6.286228319584305E-5 5 0.0 0.0 0.0 0.0 6.286228319584305E-5 6 6.286228319584305E-5 0.0 0.0 0.0 6.286228319584305E-5 7 6.286228319584305E-5 0.0 0.0 0.0 6.286228319584305E-5 8 6.286228319584305E-5 0.0 0.0 0.0 6.286228319584305E-5 9 6.286228319584305E-5 0.0 0.0 0.0 6.286228319584305E-5 10-11 6.286228319584305E-5 0.0 0.0 0.0 6.286228319584305E-5 12-13 1.257245663916861E-4 0.0 0.0 6.286228319584305E-5 2.514491327833722E-4 14-15 1.8858684958752913E-4 0.0 0.0 6.286228319584305E-5 2.514491327833722E-4 16-17 3.143114159792152E-4 0.0 0.0 6.286228319584305E-5 3.457425575771367E-4 18-19 3.7717369917505825E-4 0.0 0.0 6.286228319584305E-5 3.7717369917505825E-4 20-21 3.7717369917505825E-4 0.0 0.0 6.286228319584305E-5 5.657605487625874E-4 22-23 4.400359823709013E-4 6.286228319584305E-5 0.0 6.286228319584305E-5 5.657605487625874E-4 24-25 6.914851151542735E-4 6.286228319584305E-5 0.0 1.8858684958752913E-4 5.657605487625874E-4 26-27 6.914851151542735E-4 6.286228319584305E-5 0.0 2.2001799118545064E-4 5.97191690360509E-4 28-29 7.857785399480381E-4 6.286228319584305E-5 0.0 7.543473983501165E-4 6.286228319584304E-4 30-31 8.172096815459596E-4 9.429342479376458E-5 0.0 0.002043024203864899 6.286228319584304E-4 32-33 8.172096815459596E-4 1.257245663916861E-4 0.0 0.005280431788450815 6.286228319584304E-4 34-35 9.429342479376457E-4 1.257245663916861E-4 0.0 0.01052943243530371 6.286228319584304E-4 36-37 0.0011315210975251747 1.257245663916861E-4 0.0 0.02027308633065938 6.286228319584304E-4 38-39 0.0011315210975251747 1.571557079896076E-4 0.0 0.037088747085547394 6.286228319584304E-4 40-41 0.0011315210975251747 2.2001799118545064E-4 0.0 0.053967270123631256 6.286228319584304E-4 42-43 0.0011629522391230964 2.514491327833722E-4 0.0 0.07028003261295251 6.286228319584304E-4 44-45 0.001194383380721018 2.514491327833722E-4 0.0 0.09020737638603477 6.286228319584304E-4 46-47 0.0012886768055147824 2.514491327833722E-4 0.0 0.11491225368200109 6.286228319584304E-4 48-49 0.0013201079471127039 2.514491327833722E-4 0.0 0.14250879600497618 6.286228319584304E-4 50-51 0.0013201079471127039 2.514491327833722E-4 0.0 0.17491430299243327 6.286228319584304E-4 52-53 0.0013201079471127039 2.514491327833722E-4 0.0 0.20442814495288159 7.543473983501165E-4 54-55 0.0013201079471127039 2.514491327833722E-4 0.0 0.23815375988745136 8.800719647418026E-4 56-57 0.001382970230308547 2.514491327833722E-4 0.0 0.2793599865223265 8.800719647418026E-4 58-59 0.001382970230308547 2.514491327833722E-4 0.0 0.3284868608398778 8.800719647418026E-4 60-61 0.001382970230308547 3.143114159792152E-4 0.0 0.3813854721491797 8.800719647418026E-4 62-63 0.001382970230308547 3.143114159792152E-4 0.0 0.4332468557857503 0.0010686588143293317 64-65 0.001382970230308547 3.143114159792152E-4 0.0 0.48520253284711456 0.0010686588143293317 66-67 0.00144583251350439 3.143114159792152E-4 0.0 0.5497620976892453 0.0011000899559272532 68-69 0.00144583251350439 3.143114159792152E-4 0.0 0.625982616064205 0.0011315210975251747 70-71 0.0014772636551023115 3.7717369917505825E-4 0.0 0.7083950693339554 0.0011629522391230964 72-73 0.0015715570798960762 3.7717369917505825E-4 0.0 0.7981309785960211 0.0012258145223189394 74-75 0.0016344193630919192 3.7717369917505825E-4 0.0 0.8879926124244788 0.0012572456639168609 76-77 0.0016344193630919192 3.7717369917505825E-4 0.0 0.9931612122111243 0.0012572456639168609 78-79 0.0016344193630919192 3.7717369917505825E-4 0.0 1.1119394963096698 0.0012572456639168609 80-81 0.0018858684958752913 3.7717369917505825E-4 0.0 1.243510255038569 0.0012572456639168609 82-83 0.0018858684958752913 3.7717369917505825E-4 0.0 1.3852018413619993 0.0012572456639168609 84-85 0.0018858684958752913 3.7717369917505825E-4 0.0 1.5286221404733151 0.0012886768055147824 86-87 0.0018858684958752913 3.7717369917505825E-4 0.0 1.683294788276687 0.0014144013719064683 88-89 0.0018858684958752913 3.7717369917505825E-4 0.0 1.8589005763842747 0.001508694796700233 90-91 0.0018858684958752913 3.7717369917505825E-4 0.0 2.063800188461125 0.001508694796700233 92-93 0.0019487307790711343 3.7717369917505825E-4 0.0 2.2764947236542596 0.0016344193630919192 94-95 0.0019487307790711343 3.7717369917505825E-4 0.0 2.4889692408562096 0.0016658505046898407 96-97 0.0019487307790711343 3.7717369917505825E-4 0.0 2.710621651404752 0.0018544373542773696 98-99 0.0020115930622669775 3.7717369917505825E-4 0.0 2.963830928117608 0.0019487307790711343 100-101 0.002105886487060742 3.7717369917505825E-4 0.0 3.2519602031457544 0.0019487307790711343 102-103 0.002325904478246193 3.7717369917505825E-4 0.0 3.548324437272556 0.002043024203864899 104-105 0.003017389593400466 3.7717369917505825E-4 0.0 3.8539608581707454 0.002168748770256585 106-107 0.0032059764429879954 3.7717369917505825E-4 0.0 4.159188674228161 0.0022001799118545064 108-109 0.003237407584585917 3.7717369917505825E-4 0.0 4.489089936439946 0.0022001799118545064 110-111 0.0033317010093796813 3.7717369917505825E-4 0.0 4.862177587207274 0.0022001799118545064 112-113 0.0033317010093796813 3.7717369917505825E-4 0.0 5.256041222570828 0.0022001799118545064 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2560 0.0 44.511658 1 GGTATCA 1580 0.0 40.40239 1 ATCAACG 2790 0.0 39.1032 3 TCAACGC 2890 0.0 37.961216 4 CAACGCA 2890 0.0 37.527306 5 TATCAAC 3155 0.0 35.930393 2 AACGCAG 3100 0.0 34.792904 6 GTACATG 5920 0.0 31.643776 1 TACATGG 5870 0.0 31.102203 2 ACGCAGA 3745 0.0 28.800535 7 CGCAGAG 3880 0.0 27.798454 8 ACATGGG 6245 0.0 27.493212 3 GCAGAGT 4465 0.0 22.94651 9 GAGTACA 4520 0.0 22.438347 1 CATGGGG 4450 0.0 21.973742 4 ATGGGGA 2395 0.0 21.397684 5 TACCGTG 205 5.870068E-5 20.347824 7 AGTACAT 4555 0.0 20.171589 2 AGAGTAC 4030 0.0 20.00561 10-11 TAAGGTA 520 0.0 19.49242 4 >>END_MODULE