FastQCFastQC Report
Fri 27 May 2016
SRR940039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940039_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590779
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27020.16985388919516792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21210.1333309026583831No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24800.052.4213451
GTATCAA41350.051.919481
ATCAACG50600.042.062773
TCAACGC51350.041.4484184
CAACGCA52000.040.865955
TATCAAC53600.039.819742
AACGCAG54450.039.452056
ACGCAGA64650.033.1282587
CGCAGAG65400.032.5664148
GTACATG64200.032.2327841
TACATGG64300.031.895372
ACATGGG65500.030.1277643
GCAGAGT76250.027.5422579
CATGGGG43600.025.0233124
AGAGTAC69550.024.46434210-11
GTGGTAT13100.023.2167931
CATGGGA26450.023.2162344
GAGTACT43400.023.16656512-13
GTACTTT43650.022.96573314-15
AGTCCGC2102.7941587E-622.6639378
Produced by FastQC (version 0.11.5)