FastQCFastQC Report
Fri 27 May 2016
SRR940038_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940038_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806212
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10450.049.074591
GTATCAA13750.044.669291
TCAACGC15600.037.4304434
CAACGCA15800.037.3175135
AACGCAG16550.035.626396
ATCAACG16400.035.604573
GTACATG33150.033.45831
TATCAAC18100.033.2790832
TACATGG33500.032.7568052
ACATGGG35650.029.7497943
ACGCAGA21100.027.661667
CATGGGG21300.026.8543784
CGCAGAG21900.026.6511868
GTATAGG2151.2050805E-724.9619521
GTAATAC2302.3302346E-723.3151473
GCAGAGT26650.021.6694189
ATGGGGA12200.021.4796645
GTATAGA3108.221832E-921.1595061
CATGGGA16700.021.0503354
GTGCATA5400.020.9813941
Produced by FastQC (version 0.11.5)