FastQCFastQC Report
Fri 27 May 2016
SRR940038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940038_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806212
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26680.3309303260184666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20220.2508025184442802No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11790.14623945066558178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15200.068.629881
GTATCAA28450.057.828991
ATCAACG34800.047.442263
TCAACGC34950.046.8975684
TATCAAC35250.046.8366132
CAACGCA35600.046.145645
AACGCAG37400.043.913836
GTGGTAT7300.037.5624351
ACGCAGA43600.037.389287
CGCAGAG45350.035.815288
TGGTATC7350.034.869522
GCAGAGT50750.032.0043959
ATAACGC1203.6514895E-529.8012753
GTACATG34800.029.462391
TATACCG850.007196593628.009975
GAGTACT31000.027.73250812-13
TACATGG36150.027.5342332
ACATGGG35600.026.620243
TAACGCA900.00944765926.490024
CAGAGTA49700.026.0965510-11
Produced by FastQC (version 0.11.5)