FastQCFastQC Report
Fri 27 May 2016
SRR940037_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940037_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544871
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29800.5469184449163197No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5690.10442838763670666No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6750.053.8862231
TATGCCG453.1430705E-452.9784663
GTATCAA12400.045.202021
GTACATG32250.038.2729841
TACATGG32800.036.7222442
TCAACGC14500.036.17154
ATCAACG14600.035.515533
CAACGCA15300.033.8657155
ACATGGG33100.033.4916733
CATGGGG16750.033.091774
TATCAAC16400.033.0863762
AACGCAG15550.032.9382446
AGGACCG1701.2461896E-831.5301485
CCGTTGC1004.4179475E-429.7702629
GTAAGGT1954.6966306E-827.5080494
TATAGGA2205.0258677E-927.1037252
GTCCAAG4850.027.0478421
GTATAGG2256.386472E-926.5014231
ACGCAGA19400.026.4015337
CATGGGA17650.026.3391514
Produced by FastQC (version 0.11.5)