##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940037_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 544871 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.513279289960376 33.0 31.0 34.0 26.0 34.0 2 30.675736091662063 33.0 31.0 34.0 26.0 34.0 3 31.06144206610372 34.0 31.0 34.0 26.0 34.0 4 34.03606725261576 37.0 35.0 37.0 28.0 37.0 5 34.416324230872995 37.0 35.0 37.0 31.0 37.0 6 34.235297896199285 37.0 35.0 37.0 30.0 37.0 7 34.23702858107699 37.0 35.0 37.0 30.0 37.0 8 34.20927155234909 37.0 35.0 37.0 30.0 37.0 9 35.69026430109145 39.0 38.0 39.0 30.0 39.0 10-11 35.78113259835814 39.0 38.0 39.0 30.0 39.0 12-13 35.726800472038335 39.0 38.0 39.0 30.0 39.0 14-15 37.13232581656943 41.0 39.0 41.0 30.0 41.0 16-17 37.19965000890119 41.0 39.0 41.0 30.0 41.0 18-19 37.18999451246258 41.0 39.0 41.0 30.0 41.0 20-21 37.17496893760175 41.0 39.0 41.0 30.0 41.0 22-23 37.238366512440564 41.0 39.0 41.0 30.0 41.0 24-25 37.337884563502186 41.0 39.0 41.0 30.0 41.0 26-27 37.386012469006424 41.0 38.0 41.0 30.0 41.0 28-29 37.354178328448384 41.0 38.0 41.0 30.0 41.0 30-31 37.29737497499408 41.0 38.0 41.0 30.0 41.0 32-33 37.23370944682319 41.0 38.0 41.0 30.0 41.0 34-35 37.14671454344239 41.0 38.0 41.0 30.0 41.0 36-37 37.00350541687849 41.0 38.0 41.0 30.0 41.0 38-39 36.875506312503326 40.0 38.0 41.0 30.0 41.0 40-41 36.74927551659017 40.0 38.0 41.0 29.5 41.0 42-43 36.57677964141971 40.0 37.0 41.0 28.0 41.0 44-45 36.40056086670056 40.0 37.0 41.0 28.0 41.0 46-47 36.214977490084806 40.0 36.5 41.0 28.0 41.0 48-49 36.037766737447946 40.0 36.0 41.0 28.0 41.0 50-51 35.84833015521105 40.0 35.0 41.0 28.0 41.0 52-53 35.948258395106365 40.0 35.0 41.0 28.0 41.0 54-55 35.720955969394595 40.0 35.0 41.0 28.0 41.0 56-57 35.5629222329689 39.0 35.0 41.0 28.0 41.0 58-59 35.32198722266371 39.0 35.0 41.0 28.0 41.0 60-61 35.034330144199274 39.0 35.0 41.0 27.0 41.0 62-63 34.70381705027428 38.0 35.0 41.0 27.0 41.0 64-65 34.35773788658233 37.0 34.5 40.0 26.0 41.0 66-67 34.011610087525305 37.0 34.0 40.0 26.0 41.0 68-69 33.64858287557973 36.0 34.0 39.0 26.0 41.0 70-71 33.27890363040059 35.5 34.0 39.0 25.0 41.0 72-73 32.91617924242619 35.0 34.0 38.5 24.5 40.5 74-75 32.55843860289866 35.0 33.5 37.0 24.0 39.5 76-77 32.21182445019096 35.0 33.0 37.0 23.0 39.0 78-79 31.892186407424877 35.0 33.0 36.0 21.5 39.0 80-81 31.59802320182208 35.0 33.0 36.0 20.0 37.5 82-83 31.317682534030993 35.0 33.0 35.5 19.0 37.0 84-85 31.09886927364459 35.0 33.0 35.0 18.0 37.0 86-87 30.905306944212484 35.0 33.0 35.0 17.0 36.0 88-89 30.74245555369987 35.0 33.0 35.0 14.0 36.0 90-91 30.59890873252568 35.0 32.0 35.0 10.5 36.0 92-93 30.455420640848935 35.0 32.0 35.0 7.0 35.5 94-95 30.34247097019294 35.0 32.0 35.0 7.0 35.0 96-97 30.241576446535053 35.0 32.0 35.0 6.0 35.0 98-99 30.17289964046536 35.0 32.0 35.0 3.5 35.0 100-101 30.055966458115776 35.0 31.5 35.0 2.0 35.0 102-103 29.36677488800101 33.5 30.0 34.5 2.0 35.0 104-105 30.286240229338688 35.0 32.5 35.0 2.0 35.0 106-107 30.407408359042783 35.0 33.0 35.0 2.0 35.0 108-109 30.388575460980675 35.0 33.0 35.0 2.0 35.0 110-111 30.326012946183592 35.0 33.0 35.0 2.0 35.0 112-113 30.2679891203606 35.0 33.0 35.0 2.0 35.0 114-115 30.192725434093575 35.0 33.0 35.0 2.0 35.0 116-117 30.10536347135377 35.0 33.0 35.0 2.0 35.0 118-119 30.013980373336075 35.0 32.5 35.0 2.0 35.0 120-121 29.907894712693462 35.0 32.0 35.0 2.0 35.0 122-123 29.750968577883572 35.0 32.0 35.0 2.0 35.0 124-125 28.449337549621838 34.0 28.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09213764488668374 1101 2 0.005447660142269939 1101 3 0.23748264515075235 1101 4 0.12344003696571804 1101 5 0.19916909284930995 1101 6 0.3604515865776463 1101 7 0.2703598013782553 1101 8 0.21785877860128977 1101 9 0.3029227654436326 1101 10-11 0.3638311338738802 1101 12-13 0.2873752100986948 1101 14-15 0.5143137688390027 1101 16-17 0.4505002975477481 1101 18-19 0.44360169579693576 1101 20-21 0.40375770903332864 1101 22-23 0.42096787475795594 1101 24-25 0.362756044721003 1101 26-27 0.3827288180935611 1101 28-29 0.29759506705222805 1101 30-31 0.4543376228759328 1101 32-33 0.3929138688095506 1101 34-35 0.5182337216354469 1101 36-37 0.48820689195623146 1101 38-39 0.5395489466829986 1101 40-41 0.5525255964167215 1101 42-43 0.5083486978304421 1101 44-45 0.5258017676727533 1101 46-47 0.49500781265334837 1101 48-49 0.44244305892112834 1101 50-51 0.44123826553587975 1101 52-53 0.5522946565471898 1101 54-55 0.6549810204757023 1101 56-57 0.6050666818177604 1101 58-59 0.5742791159729492 1101 60-61 0.46237599167140786 1101 62-63 0.5473144346715841 1101 64-65 0.510468691779252 1101 66-67 0.4980217897795711 1101 68-69 0.5397675372999089 1101 70-71 0.5108630315027582 1101 72-73 0.45717555597190085 1101 74-75 0.574574627852094 1101 76-77 0.5662449320743619 1101 78-79 0.4847098996640504 1101 80-81 0.5326492763583381 1101 82-83 0.48864962178208415 1101 84-85 0.5819571069518794 1101 86-87 0.5290904902015434 1101 88-89 0.5363047306213744 1101 90-91 0.5209288967968817 1101 92-93 0.5649969987377936 1101 94-95 0.6664412419547467 1101 96-97 0.5486534102705285 1101 98-99 0.6398077057736025 1101 100-101 0.5353101290203206 1101 102-103 0.4057527936946137 1101 104-105 0.4525658248249904 1101 106-107 0.5296554774500848 1101 108-109 0.4379412161968794 1101 110-111 0.41408367203599994 1101 112-113 0.44242792573719214 1101 114-115 0.29878693845138216 1101 116-117 0.36660247108176236 1101 118-119 0.3581493704271921 1101 120-121 0.6155520853022551 1101 122-123 0.3324215151204477 1101 124-125 0.36902155526423286 1102 1 0.3307224435253673 1102 2 0.36265129031810517 1102 3 0.29108446023867174 1102 4 0.39052738807178855 1102 5 0.4285720990886759 1102 6 0.4803821027432704 1102 7 0.6105115313839278 1102 8 0.46498725279300857 1102 9 0.5636643990227626 1102 10-11 0.458201525252214 1102 12-13 0.694227876009073 1102 14-15 0.7034799628265205 1102 16-17 0.66444669573265 1102 18-19 0.45374066346568753 1102 20-21 0.3849864384717847 1102 22-23 0.2825674209859699 1102 24-25 0.4181658575400533 1102 26-27 0.3337888010771124 1102 28-29 0.4533310284816068 1102 30-31 0.5297417635452533 1102 32-33 0.47390153188384687 1102 34-35 0.37582731210623166 1102 36-37 0.45942073200159683 1102 38-39 0.5106032575168058 1102 40-41 0.33714343532767543 1102 42-43 0.27368122760923086 1102 44-45 0.14975596506356226 1102 46-47 0.3393959294996378 1102 48-49 0.2873629398059805 1102 50-51 0.41037822583083283 1102 52-53 0.35687137237248834 1102 54-55 0.3139252916044626 1102 56-57 0.346523006264718 1102 58-59 0.3041429843790766 1102 60-61 0.2820016297882546 1102 62-63 0.41247679995802855 1102 64-65 0.35841962768396485 1102 66-67 0.42437247043754667 1102 68-69 0.5677737766646302 1102 70-71 0.39430035424808096 1102 72-73 0.454587637650846 1102 74-75 0.42764822399503544 1102 76-77 0.3686059644056101 1102 78-79 0.3534350840089253 1102 80-81 0.44388013285295 1102 82-83 0.4742033654009177 1102 84-85 0.6211481165945365 1102 86-87 0.5708694181652412 1102 88-89 0.6444123596627804 1102 90-91 0.5601199064395388 1102 92-93 0.610108314676534 1102 94-95 0.7587732045185582 1102 96-97 0.685369944587034 1102 98-99 0.6101707233572675 1102 100-101 0.5066834982772654 1102 102-103 0.5584399746361903 1102 104-105 0.668408848080805 1102 106-107 0.7911415806832132 1102 108-109 0.6779617777397036 1102 110-111 0.6466418115708805 1102 112-113 0.7295805065653269 1102 114-115 0.6805403417412279 1102 116-117 0.710760156844664 1102 118-119 0.7159024901095528 1102 120-121 0.5749430382234095 1102 122-123 0.6377958770036045 1102 124-125 0.7915258093765445 1103 1 0.01307511169966702 1103 2 0.15502817626673604 1103 3 0.24550947795899347 1103 4 -0.14134809712752627 1103 5 -0.024099872423796853 1103 6 0.12078823566256602 1103 7 0.1897994275597128 1103 8 0.17802772263470956 1103 9 0.08135518783026896 1103 10-11 0.11889215373299322 1103 12-13 0.181954442991902 1103 14-15 0.29142866671148937 1103 16-17 0.37863160308068444 1103 18-19 0.2923496603266571 1103 20-21 0.17944335277292822 1103 22-23 0.24039621735415295 1103 24-25 0.38507210015658444 1103 26-27 0.3878252374358411 1103 28-29 0.2561323161583928 1103 30-31 0.2635709143198781 1103 32-33 0.3501546118446939 1103 34-35 0.2821036816340339 1103 36-37 0.2467990102612987 1103 38-39 0.09925128127891014 1103 40-41 0.0510498804621804 1103 42-43 0.17006902421367442 1103 44-45 0.23020833958680242 1103 46-47 0.08601324647401754 1103 48-49 0.07858062131271737 1103 50-51 -0.03055719625037767 1103 52-53 -0.13712797788234354 1103 54-55 -0.0686596393706651 1103 56-57 -0.06342822065666098 1103 58-59 0.11450177872207234 1103 60-61 0.023868212634987174 1103 62-63 0.15100394580471743 1103 64-65 0.12439639718475348 1103 66-67 0.023845712750301118 1103 68-69 0.17265277217548203 1103 70-71 0.013020187508281822 1103 72-73 0.16083320757017816 1103 74-75 0.18667925987447376 1103 76-77 0.20080611123114522 1103 78-79 0.1237037578537219 1103 80-81 0.2122839536920509 1103 82-83 0.19000630697448173 1103 84-85 0.2623041988843404 1103 86-87 0.15867361703789484 1103 88-89 0.09880362438752499 1103 90-91 0.19193273764080487 1103 92-93 0.10126819417834199 1103 94-95 0.18675169542212444 1103 96-97 0.16873288211023763 1103 98-99 0.3034561977556187 1103 100-101 0.17634609489521935 1103 102-103 0.20000214765404323 1103 104-105 0.2671068262763683 1103 106-107 0.21165749522062782 1103 108-109 0.2271251253618054 1103 110-111 0.1785067489590766 1103 112-113 0.2164442625986709 1103 114-115 0.340324913246949 1103 116-117 0.3190587527200499 1103 118-119 0.19078393271258065 1103 120-121 0.23454302303456132 1103 122-123 0.1945051286996886 1103 124-125 0.270079999810946 1104 1 -0.117702632164157 1104 2 -0.22758559293704295 1104 3 0.0844853319158183 1104 4 -0.30201095574163617 1104 5 -0.13909724582266136 1104 6 -0.010836664931481721 1104 7 0.25918530122123684 1104 8 -0.03124490610508701 1104 9 0.09954206479113736 1104 10-11 -0.009074192550620808 1104 12-13 0.13768946565541995 1104 14-15 0.23218271925597378 1104 16-17 -0.08046401558251404 1104 18-19 -0.006463074432311089 1104 20-21 -0.3665315107580156 1104 22-23 -0.26194696002343676 1104 24-25 -0.20246016135737221 1104 26-27 0.010530075988121723 1104 28-29 -0.041045281529370925 1104 30-31 0.08938573167247199 1104 32-33 0.17364701546504335 1104 34-35 0.3195592214784284 1104 36-37 0.31212455307489506 1104 38-39 0.02185271687734769 1104 40-41 0.03628069952569746 1104 42-43 0.10947966890410044 1104 44-45 0.030655173234706012 1104 46-47 -0.094634620788149 1104 48-49 -0.16308225598867665 1104 50-51 -0.007861493702058908 1104 52-53 -0.2511702235951745 1104 54-55 -0.08823664238761353 1104 56-57 -0.1656663339887956 1104 58-59 -0.09684513818804419 1104 60-61 -0.09838916936003272 1104 62-63 -0.046325434130537246 1104 64-65 -0.14896529928812186 1104 66-67 -0.2860599995361781 1104 68-69 -0.22710761535719826 1104 70-71 -0.24550276981619135 1104 72-73 -0.23978264215085687 1104 74-75 -0.14288651001034935 1104 76-77 -0.17968457523573278 1104 78-79 -0.0916775353476389 1104 80-81 -0.13375569241237528 1104 82-83 -0.046439704433066 1104 84-85 -0.018591586303291052 1104 86-87 -0.13518491194088966 1104 88-89 -0.12979165271370263 1104 90-91 0.001478200680480768 1104 92-93 -0.2060565969912993 1104 94-95 -0.19809520383889634 1104 96-97 -0.1888679617243092 1104 98-99 -0.14031481245801558 1104 100-101 -0.18162434182653442 1104 102-103 -0.15844170018165826 1104 104-105 -0.2621605700247933 1104 106-107 -0.16294981233671635 1104 108-109 -0.18454321277831198 1104 110-111 -0.15163521208417308 1104 112-113 -0.11456769518468235 1104 114-115 -0.17495712972964128 1104 116-117 -0.12858883008017585 1104 118-119 -0.08792232607303774 1104 120-121 -0.028621647207604184 1104 122-123 -0.0034066714875962134 1104 124-125 0.1805990701810174 1105 1 -0.21030357287081713 1105 2 -0.14578939899091026 1105 3 -0.08130135204173783 1105 4 -0.07599846101638263 1105 5 -0.12140907875974705 1105 6 0.10150264816739707 1105 7 0.047785665564951785 1105 8 0.09375586580956963 1105 9 0.13894882264665398 1105 10-11 0.12440426073823829 1105 12-13 0.16732893004957106 1105 14-15 0.292942694966392 1105 16-17 0.169502534782346 1105 18-19 0.22152655103469954 1105 20-21 0.13990111138461714 1105 22-23 0.05394624815679805 1105 24-25 -0.17767758398579048 1105 26-27 -0.08258856214857957 1105 28-29 0.0639937773522874 1105 30-31 -0.07492299302158756 1105 32-33 0.08637434433156699 1105 34-35 -0.05586919115626898 1105 36-37 -0.003852021888306467 1105 38-39 0.17686141378248976 1105 40-41 0.06760499631144512 1105 42-43 0.18703136145563803 1105 44-45 0.0541038820787918 1105 46-47 0.13138919535206384 1105 48-49 0.17447499434839386 1105 50-51 0.15479327518669095 1105 52-53 0.23880106117958633 1105 54-55 0.1934833893123411 1105 56-57 0.06350895239154397 1105 58-59 0.042071711165093006 1105 60-61 0.08850886585986473 1105 62-63 0.03681697897760472 1105 64-65 0.1617375547376696 1105 66-67 0.13481771888994842 1105 68-69 0.15889808651906634 1105 70-71 0.19658201728974234 1105 72-73 0.0728932257385182 1105 74-75 0.17053554204756693 1105 76-77 0.15378770273588316 1105 78-79 0.042652082503146005 1105 80-81 0.08058751113637186 1105 82-83 -0.056994350844689734 1105 84-85 -0.045332768464859896 1105 86-87 -0.037792954262396705 1105 88-89 -0.11957459031212281 1105 90-91 -0.08774587070618622 1105 92-93 0.02645953602503681 1105 94-95 -0.04965807371467079 1105 96-97 0.0166785023919509 1105 98-99 -0.025489934163228867 1105 100-101 0.17831328746916952 1105 102-103 -0.05126598152910944 1105 104-105 -0.1258064773332812 1105 106-107 -0.054688267066495655 1105 108-109 -0.03194494804246162 1105 110-111 0.04906568642448761 1105 112-113 -0.08028439221332917 1105 114-115 -0.14012010664425034 1105 116-117 0.01914019699859182 1105 118-119 -0.05193463553876754 1105 120-121 -0.028293326173447042 1105 122-123 -0.12622035187376213 1105 124-125 -0.20909741297580808 1106 1 -0.03664573034261309 1106 2 -0.029507160158022572 1106 3 0.16708044391425503 1106 4 -0.08143121787496455 1106 5 0.02226339273381228 1106 6 -0.012382845109286222 1106 7 0.11034553410465975 1106 8 0.08970384280402044 1106 9 0.20387136928900418 1106 10-11 -0.10381060469531889 1106 12-13 -0.07414518870557174 1106 14-15 0.009050588595769682 1106 16-17 0.11807069974898354 1106 18-19 0.24559019705382212 1106 20-21 -0.19938066653566722 1106 22-23 0.07619029136233735 1106 24-25 0.12840546044217405 1106 26-27 0.07812465001149604 1106 28-29 0.25751294530483193 1106 30-31 0.24735812358930076 1106 32-33 0.12592264997674363 1106 34-35 0.14077516466826268 1106 36-37 0.16012966184548105 1106 38-39 0.09187714493015875 1106 40-41 0.08080112464554645 1106 42-43 0.21008200172336444 1106 44-45 0.3056759948620993 1106 46-47 0.32279417721616 1106 48-49 0.07492488513214113 1106 50-51 0.1448696263620235 1106 52-53 0.1695920272924809 1106 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-1.3912033710945266 2216 58-59 -1.3422259328286117 2216 60-61 -1.272441371357445 2216 62-63 -1.2686809908401457 2216 64-65 -1.360249971922073 2216 66-67 -1.3272411905943926 2216 68-69 -1.4156285670068556 2216 70-71 -1.7234648665514989 2216 72-73 -1.3730703491232816 2216 74-75 -1.3298729816088724 2216 76-77 -1.2485179376265023 2216 78-79 -1.214076121795987 2216 80-81 -1.222689383337677 2216 82-83 -1.3704134513464865 2216 84-85 -1.2493639082893857 2216 86-87 -1.317517247429418 2216 88-89 -1.2614237039339322 2216 90-91 -1.307529110665648 2216 92-93 -1.3731288313104812 2216 94-95 -1.2309437107193268 2216 96-97 -1.4443768192971085 2216 98-99 -1.5756879779753703 2216 100-101 -1.512382073269034 2216 102-103 -1.553018840262002 2216 104-105 -1.401285730922634 2216 106-107 -1.563713095604701 2216 108-109 -1.4614562741587491 2216 110-111 -1.4342849537202653 2216 112-113 -1.4280331594916404 2216 114-115 -1.51397459263195 2216 116-117 -1.4234519695447538 2216 118-119 -1.5799797534634443 2216 120-121 -1.705697677541096 2216 122-123 -1.6871096525334934 2216 124-125 -1.4807476122632224 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12728.0 3 2706.0 4 2489.0 5 1839.0 6 1830.0 7 1764.0 8 1844.0 9 1820.0 10 2064.0 11 2465.0 12 2628.0 13 2444.0 14 2436.0 15 2378.0 16 2129.0 17 2197.0 18 1700.0 19 1531.0 20 1398.0 21 1265.0 22 1259.0 23 2132.0 24 4266.0 25 5863.0 26 6006.0 27 4444.0 28 4480.0 29 5315.0 30 5914.0 31 7221.0 32 9686.0 33 13122.0 34 20720.0 35 38408.0 36 94339.0 37 191927.0 38 77919.0 39 195.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.297159183763405 19.296837845232346 13.048731447290383 23.357271523713866 2 15.43171850721293 20.99353640484356 38.96527311677684 24.609471971166666 3 18.963918897832933 26.293036191245445 27.031363298424033 27.71168161249759 4 12.772662375486249 17.5850809511647 34.46970601800385 35.172550655345205 5 14.22130846611352 39.714553197738454 31.373448858212793 14.690689477935237 6 32.05894395147002 37.04835923055597 16.44771992442895 14.444976893545064 7 29.790925732457644 33.97369633830849 18.748014740038816 17.487363189195047 8 26.404740033069995 37.27448098475106 18.008267499540693 18.312511482638254 9 26.3294163045176 19.499085822438236 18.025008958021335 36.146488915022836 10-11 24.965548393375375 29.221939958263288 24.728679252558507 21.083832395802826 12-13 25.251725059047185 26.31783312205166 25.545652906278143 22.884788912623016 14-15 22.766954114766317 27.426880619096394 23.008513309617946 26.797651956519346 16-17 23.06751777580728 30.28936976432942 23.86547849186985 22.77763396799345 18-19 23.328286663110433 28.856731795950484 25.328530886448085 22.486450654491 20-21 24.37559424522351 27.80423976151112 25.266525304501826 22.553640688763547 22-23 24.278225591595024 27.57313288586658 25.131511495657318 23.017130026881077 24-25 23.764837697969902 27.762486625982103 25.215430746694956 23.257244929353043 26-27 23.428735058298336 27.95520793562219 25.241747187876296 23.37430981820318 28-29 23.41398503209702 27.865169570464314 25.412489159512326 23.308356237926336 30-31 23.519559635182933 27.593282931458223 25.350252651788384 23.53690478157046 32-33 23.284975919182425 27.849043374838484 25.24842520263127 23.61755550334782 34-35 23.83787305899196 27.775595609559122 25.169964391909254 23.216566939539664 36-37 24.01291368150963 27.504985330784613 24.93564724873061 23.546453738975146 38-39 23.528575007731906 27.72986699289032 25.14874164977272 23.59281634960505 40-41 23.529017693267747 27.440716540635783 25.527310770134353 23.502954995962117 42-43 23.324960468268614 27.48490085175624 25.31260582711028 23.877532852864867 44-45 23.285427163349027 27.64553105182493 25.39752025917054 23.671521525655496 46-47 23.666315759524284 27.804970276910705 25.063213764860397 23.46550019870461 48-49 23.571048332323734 27.673513592892412 25.203021458597206 23.552416616186648 50-51 23.46225925048962 27.699982929797418 25.27830854482947 23.55944927488349 52-53 23.74407348058084 27.62405079323124 25.129818996800644 23.502056729387277 54-55 23.60652728574313 27.564657941592174 25.102149452082458 23.726665320582242 56-57 23.534600015051865 27.8467756886687 25.123030637887013 23.495593658392426 58-59 23.58353586454517 27.389990674997982 25.131614693853578 23.894858766603278 60-61 23.49333957389792 27.704711167398553 25.33008456882746 23.47186468987606 62-63 23.257974036654137 27.801192434210524 25.346385984492482 23.594447544642858 64-65 23.244516940961134 27.678972205826874 25.19999522673469 23.8765156264773 66-67 23.398697129996027 27.929185701658003 24.904239880819613 23.767877287526357 68-69 23.10802065404475 27.70992541594951 25.488835341365462 23.693218588640274 70-71 23.322499249630322 27.64178315371885 25.29438815269541 23.741329443955415 72-73 23.18318235634241 27.886872803032386 24.98040511394403 23.949539726681166 74-75 23.058151742973777 28.112335173710022 25.023494170508943 23.806018912807257 76-77 23.333715727888034 27.8521509693702 25.209865779511297 23.60426752323047 78-79 23.123769487418365 27.80662423071451 25.34647103906892 23.7231352427982 80-81 23.358080939264806 28.16509458639619 25.10268160762455 23.374142866714454 82-83 23.39085476275049 27.877287617243397 25.114478383342192 23.617379236663922 84-85 23.343059034301223 27.7962614026119 25.128929019515343 23.73175054357154 86-87 23.35726893637254 27.86816479125725 25.257124918881164 23.51744135348905 88-89 23.20704578936982 27.895120910188215 25.210359403182352 23.687473897259615 90-91 23.31674702809575 27.841219400928725 25.246431007120083 23.59560256385544 92-93 23.2479238171472 27.564786910707845 25.301479099560392 23.885810172584566 94-95 23.20321851065861 27.599848730733363 25.39396097840374 23.802971780204288 96-97 23.281820534991436 27.436898657840096 25.397957478993753 23.88332332817471 98-99 22.938489034618676 27.934711667062917 25.100538561736325 24.026260736582085 100-101 22.860586101565485 27.805772167885568 25.49573016712192 23.837911563427028 102-103 22.87979467804162 27.525699667067798 25.53994773322465 24.05455792166593 104-105 22.54849318438195 28.100392571522452 25.417285079253187 23.933829164842408 106-107 22.674010327022376 28.1045553643144 25.25907056798623 23.96236374067699 108-109 22.84181213067989 28.127896965769683 25.19141773030247 23.83887317324796 110-111 22.456738067746894 28.60188742627528 25.270346921505315 23.671027584472515 112-113 22.51821336880087 28.433599798797005 25.430975531622924 23.617211300779203 114-115 22.361508810572687 28.745502936857566 25.09480543318649 23.79818281938326 116-117 22.22013177179746 28.699967236013585 25.531497020496307 23.54840397169265 118-119 22.263212583622785 28.61881962445934 25.242607048179945 23.875360743737932 120-121 22.139002092741492 28.859180526489702 25.36118148107354 23.640635899695265 122-123 22.13077740636926 28.809430717014244 25.253178846969288 23.80661302964721 124-125 22.427549367390675 28.867727975696056 25.03535912109294 23.669363535820327 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2482.0 1 2156.0 2 1631.5 3 1189.0 4 742.5 5 469.5 6 366.0 7 364.5 8 389.0 9 361.0 10 305.0 11 278.5 12 263.0 13 240.0 14 240.0 15 248.5 16 298.5 17 343.0 18 373.0 19 445.5 20 538.0 21 647.5 22 771.5 23 950.5 24 1157.0 25 1466.5 26 1925.0 27 2375.0 28 2772.5 29 3384.0 30 4274.5 31 5230.5 32 6464.5 33 7377.0 34 8070.0 35 8776.0 36 9708.0 37 11020.5 38 11892.0 39 12530.0 40 12954.0 41 13570.0 42 14252.5 43 15161.5 44 16349.5 45 17420.0 46 18160.5 47 18771.5 48 19691.0 49 20115.5 50 20387.5 51 20987.0 52 21516.0 53 22274.5 54 22850.5 55 22089.5 56 20401.5 57 18621.5 58 17216.5 59 15484.5 60 13301.5 61 11314.0 62 9218.5 63 7124.5 64 5578.0 65 4368.0 66 3177.5 67 2441.5 68 1802.0 69 1211.0 70 948.0 71 739.0 72 556.0 73 443.5 74 341.5 75 232.0 76 146.0 77 89.5 78 74.5 79 49.0 80 27.0 81 26.0 82 20.0 83 13.0 84 13.0 85 10.0 86 7.0 87 5.5 88 4.5 89 3.0 90 4.5 91 4.0 92 3.5 93 4.5 94 2.0 95 2.5 96 2.5 97 1.0 98 1.5 99 2.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05028713218358107 2 0.19380734155423943 3 0.023124739617267204 4 0.12002841039438694 5 0.020371794424735395 6 0.04000947013146231 7 0.042578885644492 8 0.1047954469957109 9 0.12223076654841238 10-11 0.048910659587315164 12-13 0.03294357747063066 14-15 0.014131785321663294 16-17 0.031200045515360517 18-19 0.0527647828568597 20-21 0.20445206296536245 22-23 0.11415546065031906 24-25 0.08047776446167992 26-27 0.0159670821166845 28-29 0.007065892660831647 30-31 0.009176483975106033 32-33 0.007157657500582707 34-35 0.009360013654608156 36-37 0.004129417788797715 38-39 0.009268248814857095 40-41 0.005689420064565741 42-43 0.010185897212367697 44-45 0.010277662052118759 46-47 0.016976495353946165 48-49 0.01853649762971419 50-51 0.011378840129131483 52-53 0.01358119628315693 54-55 0.014865904039671776 56-57 0.01615061179618662 58-59 0.018169438270709947 60-61 0.008901189455852854 62-63 0.018903556988718432 64-65 0.03129181035511158 66-67 0.027621216765069165 68-69 0.033677696188639146 70-71 0.025602390290545837 72-73 0.01578355243718238 74-75 0.010094132372616638 76-77 0.01073648625087406 78-79 0.02358356381602251 80-81 0.01917885150797161 82-83 0.02156473734149918 84-85 0.017343554712950404 86-87 0.02670356836755856 88-89 0.027254157406064922 90-91 0.026520038688056437 92-93 0.03331063682963491 94-95 0.026978862886811738 96-97 0.027896511284322342 98-99 0.03294357747063066 100-101 0.02184003186075236 102-103 0.030374161957600974 104-105 0.02376709349552463 106-107 0.033677696188639146 108-109 0.022023561540254484 110-111 0.019821205386229036 112-113 0.0270706277265628 114-115 0.01303060724465057 116-117 0.012021194007388904 118-119 0.029915337758845674 120-121 0.02404238801477781 122-123 0.017985908591207828 124-125 0.01899532182846949 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 544871.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.62340214581529 #Duplication Level Percentage of deduplicated Percentage of total 1 74.49510851407797 41.436713787747074 2 13.167445775344946 14.648362631904572 3 4.994191320772119 8.33381736685344 4 2.4672349236080646 5.489440013762049 5 1.3765269218511897 3.8283555269334997 6 0.8343013888231778 2.78440089967943 7 0.5700010003392302 2.2193776405770196 8 0.38289613484369234 1.7038388550791217 9 0.2769740585566125 1.3865615498747743 >10 1.3511739005048071 13.035460095896143 >50 0.05774729849868816 2.1818426479885553 >100 0.02573879371001452 2.300414914361811 >500 3.2998453474377033E-4 0.10443916749954972 >1k 3.2998453474377033E-4 0.5469749018429844 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2980 0.5469184449163197 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 569 0.10442838763670666 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 9.176483975106034E-5 0.0 22-23 0.0 0.0 0.0 5.50589038506362E-4 0.0 24-25 0.0 0.0 0.0 0.0013764725962659053 0.0 26-27 0.0 0.0 0.0 0.0020188264745233274 0.0 28-29 0.0 0.0 0.0 0.004037652949046655 0.0 30-31 0.0 0.0 0.0 0.009360013654608155 0.0 32-33 0.0 0.0 0.0 0.017802378911705705 0.0 34-35 0.0 0.0 0.0 0.02734592224581598 0.0 36-37 0.0 0.0 0.0 0.047901246350053495 0.0 38-39 0.0 0.0 0.0 0.07827540830765448 0.0 40-41 0.0 0.0 0.0 0.11388016613106589 0.0 42-43 0.0 0.0 0.0 0.149026099755722 0.0 44-45 0.0 0.0 0.0 0.18527321145739084 0.0 46-47 0.0 0.0 0.0 0.2328073984484401 0.0 48-49 0.0 0.0 0.0 0.2869486539015657 0.0 50-51 0.0 0.0 0.0 0.3513675714068101 0.0 52-53 0.0 0.0 0.0 0.40899589077047593 0.0 54-55 0.0 0.0 0.0 0.4659818562558844 0.0 56-57 0.0 0.0 0.0 0.536732547703952 0.0 58-59 0.0 0.0 0.0 0.6228079673904465 0.0 60-61 0.0 0.0 0.0 0.7108104487117135 0.0 62-63 0.0 0.0 0.0 0.8039517610590396 0.0 64-65 0.0 0.0 0.0 0.8890177675082727 0.0 66-67 0.0 0.0 0.0 0.9941802738629877 0.0 68-69 0.0 0.0 0.0 1.1274228211815274 0.0 70-71 0.0 0.0 0.0 1.2765406857770003 0.0 72-73 0.0 0.0 0.0 1.426392669090482 0.0 74-75 0.0 0.0 0.0 1.5655081661530894 0.0 76-77 0.0 0.0 0.0 1.7183883891783558 0.0 78-79 0.0 0.0 0.0 1.8975133563724258 0.0 80-81 0.0 0.0 0.0 2.0998548280235134 0.0 82-83 0.0 0.0 0.0 2.3108221946112018 0.0 84-85 0.0 0.0 0.0 2.5288554538597205 0.0 86-87 0.0 0.0 0.0 2.7326651629468257 0.0 88-89 0.0 0.0 0.0 2.979145522518174 0.0 90-91 0.0 0.0 0.0 3.263065936707955 0.0 92-93 0.0 0.0 0.0 3.5680004992007284 0.0 94-95 0.0 0.0 0.0 3.8618315160836234 0.0 96-97 0.0 0.0 0.0 4.165664900499384 0.0 98-99 0.0 0.0 0.0 4.488310077064112 0.0 100-101 0.0 0.0 0.0 4.884183595750187 0.0 102-103 0.0 0.0 0.0 5.30327361889328 0.0 104-105 0.0 0.0 0.0 5.716215397773051 0.0 106-107 0.0 0.0 0.0 6.106950085433066 0.0 108-109 0.0 0.0 0.0 6.52879305376869 0.0 110-111 0.0 0.0 0.0 7.007530222749972 0.0 112-113 0.0 0.0 0.0 7.519578028560889 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 675 0.0 53.886223 1 TATGCCG 45 3.1430705E-4 52.978466 3 GTATCAA 1240 0.0 45.20202 1 GTACATG 3225 0.0 38.272984 1 TACATGG 3280 0.0 36.722244 2 TCAACGC 1450 0.0 36.1715 4 ATCAACG 1460 0.0 35.51553 3 CAACGCA 1530 0.0 33.865715 5 ACATGGG 3310 0.0 33.491673 3 CATGGGG 1675 0.0 33.09177 4 TATCAAC 1640 0.0 33.086376 2 AACGCAG 1555 0.0 32.938244 6 AGGACCG 170 1.2461896E-8 31.530148 5 CCGTTGC 100 4.4179475E-4 29.770262 9 GTAAGGT 195 4.6966306E-8 27.508049 4 TATAGGA 220 5.0258677E-9 27.103725 2 GTCCAAG 485 0.0 27.047842 1 GTATAGG 225 6.386472E-9 26.501423 1 ACGCAGA 1940 0.0 26.401533 7 CATGGGA 1765 0.0 26.339151 4 >>END_MODULE