FastQCFastQC Report
Fri 27 May 2016
SRR940037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940037_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544871
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53400.9800484885413244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39490.7247587043538746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25910.4755253995899947No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10910.20023088033681366No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7910.14517197648617747No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6410.11764252456085936No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5880.10791545154724695No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17650.072.926391
TATAACG453.954641E-666.1992952
GTATCAA36100.058.5998881
TATCAAC45500.046.223112
ATCAACG45400.046.189443
TCAACGC45450.046.138634
CAACGCA46300.045.378565
GTGGTAT6850.045.2364461
AACGCAG48050.043.957456
ACGCAGA55200.038.367957
CGCAGAG55550.038.2333038
TGGTATC8450.035.254062
TAACGCA851.6968789E-435.043464
GCAGAGT60800.034.3448149
GTACATG34100.032.8533131
GTATAGG1659.264113E-932.5038071
TACATGG34250.031.8336452
GTATAAC1501.4563557E-731.7814981
GAGTACT41000.030.76165412-13
ACATGGG34250.030.6131523
Produced by FastQC (version 0.11.5)