##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940037_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 544871 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.11433164914264 34.0 33.0 34.0 31.0 34.0 2 33.1459024246106 34.0 33.0 34.0 31.0 34.0 3 33.199797750293186 34.0 33.0 34.0 31.0 34.0 4 36.44951924400454 37.0 37.0 37.0 35.0 37.0 5 36.45953996450536 37.0 37.0 37.0 35.0 37.0 6 36.53985255225549 37.0 37.0 37.0 35.0 37.0 7 36.528809571439844 37.0 37.0 37.0 35.0 37.0 8 36.52492608342158 37.0 37.0 37.0 35.0 37.0 9 38.38593722183783 39.0 39.0 39.0 37.0 39.0 10-11 38.37180451886777 39.0 39.0 39.0 37.0 39.0 12-13 38.35985306613859 39.0 39.0 39.0 37.0 39.0 14-15 39.928726248965354 41.0 40.0 41.0 38.0 41.0 16-17 39.93763753255358 41.0 40.0 41.0 38.0 41.0 18-19 39.994435380117494 41.0 40.0 41.0 38.0 41.0 20-21 39.9949501808685 41.0 40.0 41.0 38.0 41.0 22-23 39.949384349690106 41.0 40.0 41.0 38.0 41.0 24-25 39.91391632147793 41.0 40.0 41.0 38.0 41.0 26-27 39.808150920126046 41.0 40.0 41.0 37.0 41.0 28-29 39.71012037711678 41.0 40.0 41.0 37.0 41.0 30-31 39.50075889522474 41.0 39.5 41.0 36.0 41.0 32-33 39.261197604570626 41.0 39.0 41.0 35.0 41.0 34-35 39.127950469010095 41.0 39.0 41.0 35.0 41.0 36-37 39.00127644892093 41.0 39.0 41.0 35.0 41.0 38-39 38.85938139486227 41.0 39.0 41.0 35.0 41.0 40-41 38.69560593241336 41.0 38.5 41.0 35.0 41.0 42-43 38.4834116699182 41.0 38.0 41.0 35.0 41.0 44-45 38.196790616494546 41.0 38.0 41.0 35.0 41.0 46-47 37.759324684191306 41.0 38.0 41.0 33.5 41.0 48-49 37.362427987542006 40.5 37.5 41.0 33.0 41.0 50-51 37.31695025060978 41.0 37.5 41.0 33.0 41.0 52-53 37.18169805330069 41.0 37.0 41.0 33.0 41.0 54-55 36.99017106801426 40.0 36.5 41.0 33.0 41.0 56-57 36.77405752921334 40.0 36.0 41.0 32.0 41.0 58-59 36.526620062363385 39.5 35.0 41.0 32.0 41.0 60-61 36.25435653576718 39.0 35.0 41.0 32.0 41.0 62-63 35.950212068544666 39.0 35.0 41.0 31.0 41.0 64-65 35.61468677907248 37.5 35.0 41.0 31.0 41.0 66-67 35.26860119184174 37.0 35.0 40.5 31.0 41.0 68-69 34.893185726529765 36.5 35.0 39.5 30.0 41.0 70-71 34.52195382026204 36.0 35.0 39.0 30.0 41.0 72-73 34.17061836654915 35.5 35.0 39.0 30.0 41.0 74-75 33.83125088323658 35.0 35.0 37.0 29.0 39.5 76-77 33.49871620989188 35.0 35.0 37.0 29.0 39.0 78-79 33.19940499677905 35.0 35.0 36.5 29.0 39.0 80-81 32.930081615648476 35.0 35.0 36.0 29.0 38.0 82-83 32.6931961877215 35.0 35.0 36.0 28.5 37.0 84-85 32.482655527638656 35.0 34.0 35.0 27.5 37.0 86-87 32.3071011303593 35.0 34.0 35.0 27.0 36.0 88-89 32.22657656582935 35.0 34.5 35.0 27.5 36.0 90-91 32.245936194071625 35.0 35.0 35.0 29.0 36.0 92-93 32.17307858190287 35.0 35.0 35.0 29.0 36.0 94-95 32.10667020267182 35.0 35.0 35.0 28.5 35.5 96-97 32.05040000293647 35.0 35.0 35.0 27.0 35.0 98-99 31.996111923739747 35.0 35.0 35.0 27.0 35.0 100-101 31.931965547808566 35.0 34.5 35.0 27.0 35.0 102-103 31.448967737317638 35.0 33.5 35.0 25.0 35.0 104-105 31.827032453553226 35.0 34.0 35.0 27.0 35.0 106-107 31.866703311426008 35.0 34.0 35.0 27.0 35.0 108-109 31.839130730026007 35.0 35.0 35.0 27.0 35.0 110-111 31.793013392160713 35.0 34.0 35.0 26.0 35.0 112-113 31.75832720038321 35.0 34.0 35.0 25.5 35.0 114-115 31.689751335637244 35.0 34.0 35.0 25.0 35.0 116-117 31.62074601144124 35.0 34.0 35.0 25.0 35.0 118-119 31.54679639767945 35.0 34.0 35.0 24.0 35.0 120-121 31.458100174169665 35.0 34.0 35.0 24.0 35.0 122-123 31.33750006882363 35.0 34.0 35.0 21.5 35.0 124-125 30.274666847749284 34.5 32.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22359473975168243 1101 2 0.18179406530521192 1101 3 0.2207935208265681 1101 4 0.17221773417017516 1101 5 0.20285193758679299 1101 6 0.11464439438395146 1101 7 0.15481600802235107 1101 8 0.18111418383939082 1101 9 0.09507196025651865 1101 10-11 0.11809209419359235 1101 12-13 0.10733509285734755 1101 14-15 0.1959564513504617 1101 16-17 0.24270180924090567 1101 18-19 0.23318410385165578 1101 20-21 0.26321187615869235 1101 22-23 0.28005674415813075 1101 24-25 0.3498843848483446 1101 26-27 0.4007619763455068 1101 28-29 0.3611685613846447 1101 30-31 0.42399601913423624 1101 32-33 0.3394625274631693 1101 34-35 0.37856228913815215 1101 36-37 0.44212062226969806 1101 38-39 0.4149898284072293 1101 40-41 0.4025227673094278 1101 42-43 0.36561647752190396 1101 44-45 0.37403662501745316 1101 46-47 0.6354728883393435 1101 48-49 0.4535804418049665 1101 50-51 0.49352742287101137 1101 52-53 0.2964872929371438 1101 54-55 0.42750334392001577 1101 56-57 0.39576519437802204 1101 58-59 0.2327401755594991 1101 60-61 0.21045990090246391 1101 62-63 0.2682679271233255 1101 64-65 0.237678976654216 1101 66-67 0.3922730701072652 1101 68-69 0.3053326307487083 1101 70-71 0.2672446819025325 1101 72-73 0.3669578220754772 1101 74-75 0.26087224920251373 1101 76-77 0.2162248090681942 1101 78-79 0.3555372999618598 1101 80-81 0.366102358537546 1101 82-83 0.19035133614641353 1101 84-85 0.376952855205019 1101 86-87 0.2970619076640233 1101 88-89 0.3706140698916016 1101 90-91 0.4657489077110242 1101 92-93 0.45551630687903355 1101 94-95 0.41018566591321104 1101 96-97 0.398191638457952 1101 98-99 0.364958440773151 1101 100-101 0.3957842909964384 1101 102-103 0.3185291256965961 1101 104-105 0.2584025918428132 1101 106-107 0.2988241648881669 1101 108-109 0.23679771325529586 1101 110-111 0.3115929816119234 1101 112-113 0.25852791923881213 1101 114-115 0.30153922383440346 1101 116-117 0.4221071680812507 1101 118-119 0.3505315543154808 1101 120-121 0.4685004889964084 1101 122-123 0.47248941079346096 1101 124-125 0.25041283482845955 1102 1 0.14283750776075976 1102 2 0.18707625475502 1102 3 0.1781460449332073 1102 4 -0.021592532420861232 1102 5 0.011416883701372171 1102 6 0.05997905121889602 1102 7 0.14531515719988874 1102 8 0.17324407606854209 1102 9 0.21361988992185843 1102 10-11 0.15946333639438848 1102 12-13 0.2135106458919509 1102 14-15 0.28071963909862774 1102 16-17 0.21804568331918261 1102 18-19 0.30683342319368734 1102 20-21 0.1934976107020816 1102 22-23 0.11609616559885438 1102 24-25 0.1380118664138621 1102 26-27 0.1798849201912276 1102 28-29 0.19539998509423384 1102 30-31 0.1895235290604944 1102 32-33 0.2782033102198369 1102 34-35 0.3474186419458647 1102 36-37 0.3616292722980603 1102 38-39 0.4308363967566322 1102 40-41 0.29188039635084095 1102 42-43 0.3530136698077868 1102 44-45 0.4986998411263599 1102 46-47 0.4194703358719565 1102 48-49 0.41220919960417035 1102 50-51 0.4925702476015914 1102 52-53 0.5980861301464486 1102 54-55 0.5990858736988045 1102 56-57 0.5586622448601517 1102 58-59 0.6125083264386788 1102 60-61 0.5723962310215782 1102 62-63 0.665548840339433 1102 64-65 0.5642175472724844 1102 66-67 0.5196660082808293 1102 68-69 0.47413226205897274 1102 70-71 0.5777771549598185 1102 72-73 0.5898378561083817 1102 74-75 0.5893960722314446 1102 76-77 0.5882646559201561 1102 78-79 0.6579337832290193 1102 80-81 0.7403224379476399 1102 82-83 0.6044537184493066 1102 84-85 0.5986984592889684 1102 86-87 0.6375858724967003 1102 88-89 0.6104928276908055 1102 90-91 0.5308013750961607 1102 92-93 0.6654922002426247 1102 94-95 0.6667263635876282 1102 96-97 0.6845642986961806 1102 98-99 0.756017714737979 1102 100-101 0.6945470533333591 1102 102-103 0.6739956600131016 1102 104-105 0.6874652690974976 1102 106-107 0.5850372959148231 1102 108-109 0.45404104847935045 1102 110-111 0.3295666061156126 1102 112-113 0.3206202334759105 1102 114-115 0.4174992351787026 1102 116-117 0.2933493688753579 1102 118-119 0.4312356099025436 1102 120-121 0.40628409648364894 1102 122-123 0.4627581288876179 1102 124-125 0.5560530787308977 1103 1 0.17290931725884917 1103 2 0.18945584094328893 1103 3 0.12095370348790624 1103 4 -0.02078246052698063 1103 5 0.04264051417357706 1103 6 0.140355481502084 1103 7 0.10366817978064091 1103 8 0.10252733120743329 1103 9 0.14448742629071631 1103 10-11 0.22469100034955858 1103 12-13 0.14369257453811457 1103 14-15 0.20414147559911555 1103 16-17 0.26418708305590854 1103 18-19 0.2192433448867419 1103 20-21 0.1924438843713716 1103 22-23 0.16528166362430596 1103 24-25 0.13349951281747963 1103 26-27 0.18998128499359979 1103 28-29 0.23464417114074365 1103 30-31 0.34591803407280963 1103 32-33 0.20694124352392151 1103 34-35 0.15724582660850217 1103 36-37 0.22241926115957256 1103 38-39 0.19518669378300046 1103 40-41 0.352766581249746 1103 42-43 0.24720963126186035 1103 44-45 0.29075712839810564 1103 46-47 0.07582475920202825 1103 48-49 -0.13913965448163168 1103 50-51 -0.22062949418799604 1103 52-53 -0.2638356388480503 1103 54-55 -0.3677792324543745 1103 56-57 -0.39548958455220884 1103 58-59 -0.32537139476157506 1103 60-61 -0.35993639631159624 1103 62-63 -0.4036585127506527 1103 64-65 -0.42960459975556375 1103 66-67 -0.2841679101044292 1103 68-69 -0.3407156806959506 1103 70-71 -0.3847496364804215 1103 72-73 -0.28002746943926127 1103 74-75 -0.31986954696726144 1103 76-77 -0.2450282441372309 1103 78-79 -0.18303264941688013 1103 80-81 -0.23171636955902386 1103 82-83 -0.390255499493783 1103 84-85 -0.5256001112716717 1103 86-87 -0.4516791982158317 1103 88-89 -0.40171415411632694 1103 90-91 -0.3253001117232941 1103 92-93 -0.22117578841637453 1103 94-95 -0.2837094719677751 1103 96-97 -0.27525697919222836 1103 98-99 -0.3133974302311344 1103 100-101 -0.2788977773953647 1103 102-103 -0.16303327474236085 1103 104-105 -0.166786131651822 1103 106-107 -0.17261619583256405 1103 108-109 -0.20685737188046005 1103 110-111 -0.17724622508565346 1103 112-113 -0.23257132321224816 1103 114-115 -0.30047655548521846 1103 116-117 -0.27484117522293516 1103 118-119 -0.34895117073673276 1103 120-121 -0.3722814855867611 1103 122-123 -0.3048345652764901 1103 124-125 -0.19272232039827486 1104 1 0.12411682705410243 1104 2 0.16423631730284427 1104 3 0.08735812531381981 1104 4 -0.14013397035544983 1104 5 -0.06634578389686396 1104 6 0.05307342191457565 1104 7 0.08717146355115801 1104 8 0.11321230310384323 1104 9 0.22512927209572808 1104 10-11 0.1614096384205368 1104 12-13 0.2075719294531737 1104 14-15 0.2136439578929128 1104 16-17 0.22978483726002707 1104 18-19 0.2705741907353598 1104 20-21 0.28507673536522304 1104 22-23 0.19803735234404485 1104 24-25 0.18279136869286106 1104 26-27 0.15630888133172505 1104 28-29 0.14093887786463455 1104 30-31 0.18210429227704594 1104 32-33 0.14215867003618854 1104 34-35 0.10304129224363123 1104 36-37 0.1733994090382538 1104 38-39 0.26847717095233037 1104 40-41 0.3347364698112685 1104 42-43 0.2558909113821315 1104 44-45 0.11172155819725305 1104 46-47 -0.037596653514874845 1104 48-49 0.11439379886557077 1104 50-51 -0.07372566516416157 1104 52-53 -0.1873288730142093 1104 54-55 -0.2998706921594021 1104 56-57 -0.2196163175379624 1104 58-59 -0.2017841012819801 1104 60-61 -0.10092134059235747 1104 62-63 -0.14278095804363744 1104 64-65 -0.022589394538073293 1104 66-67 0.03646533955141251 1104 68-69 0.023871091616143758 1104 70-71 -0.16255267669337314 1104 72-73 -0.05853024450046007 1104 74-75 -0.06986591332933045 1104 76-77 -0.11492116601816349 1104 78-79 -0.06479095643229726 1104 80-81 -0.12245599136998919 1104 82-83 -0.16006868897658677 1104 84-85 -0.10954026214031387 1104 86-87 -0.052027819821233834 1104 88-89 -0.16063265888469402 1104 90-91 -0.1042389233368155 1104 92-93 -0.027006635303180815 1104 94-95 -0.09701211663328735 1104 96-97 -0.12728901334427434 1104 98-99 -0.18761230489138825 1104 100-101 -0.13054360774392393 1104 102-103 -0.15410490724244852 1104 104-105 -0.04267974703851607 1104 106-107 -0.16640163677971742 1104 108-109 -0.1467445422316338 1104 110-111 -0.079781673819177 1104 112-113 -0.10447105141613022 1104 114-115 -0.13262739921594857 1104 116-117 -0.05617299696288569 1104 118-119 -0.018743804210636483 1104 120-121 0.06830334324812881 1104 122-123 0.09353777256463047 1104 124-125 0.0038803319497802136 1105 1 0.0824581368388948 1105 2 0.069776606101847 1105 3 0.015788256745153717 1105 4 -0.013093285306922553 1105 5 -0.015070066273537464 1105 6 0.04509491201862659 1105 7 0.018893039080431606 1105 8 0.05660111137053292 1105 9 0.031712160291611724 1105 10-11 0.06823027633080869 1105 12-13 -0.035744833445512825 1105 14-15 0.10901246995022262 1105 16-17 0.18285139688786245 1105 18-19 0.05583062078445522 1105 20-21 0.01927872999269198 1105 22-23 0.10562372959417132 1105 24-25 0.1239642672838599 1105 26-27 0.12117235535936288 1105 28-29 0.07061539416232421 1105 30-31 0.10073316600210092 1105 32-33 0.2541789250767863 1105 34-35 0.1967661406910537 1105 36-37 0.21859346838304816 1105 38-39 0.22358673137266294 1105 40-41 0.31800136004499535 1105 42-43 0.30079370159631225 1105 44-45 0.226890195819351 1105 46-47 0.33543779446322475 1105 48-49 0.399755209308033 1105 50-51 0.47492657184452014 1105 52-53 0.3570088756709566 1105 54-55 0.3930565111423334 1105 56-57 0.3185553768661791 1105 58-59 0.23352430435402738 1105 60-61 0.2995325452260289 1105 62-63 0.20974011522649505 1105 64-65 0.2459381010620092 1105 66-67 0.2514775358029979 1105 68-69 0.21224502766044395 1105 70-71 0.27451569436085066 1105 72-73 0.24543430917847786 1105 74-75 0.27301034360504417 1105 76-77 0.25737018545001433 1105 78-79 0.2478890283352584 1105 80-81 0.1530660802431072 1105 82-83 0.11598960601832431 1105 84-85 0.18000492574370952 1105 86-87 0.24186582063121165 1105 88-89 0.23513416113567587 1105 90-91 0.045222280232522394 1105 92-93 0.09297463290114649 1105 94-95 0.07758674504989926 1105 96-97 0.05475419329213338 1105 98-99 0.1800619677077009 1105 100-101 0.0342241488662367 1105 102-103 0.05098767105542734 1105 104-105 0.01760267080403466 1105 106-107 0.056745510738316796 1105 108-109 -0.008567701729788268 1105 110-111 0.04415900196637779 1105 112-113 -0.13195944696578366 1105 114-115 -0.09156153945740186 1105 116-117 0.03380549234866592 1105 118-119 -0.018895099483430045 1105 120-121 -0.005258065135894441 1105 122-123 0.015132857012094547 1105 124-125 -0.12933361191658577 1106 1 0.12136717673789121 1106 2 0.11630473179071288 1106 3 0.03585398689084229 1106 4 -0.03309088054549392 1106 5 0.018927248409539743 1106 6 -0.028627057480981932 1106 7 0.021578356007950106 1106 8 0.09146814810326731 1106 9 0.006143534412366591 1106 10-11 0.08252157309841124 1106 12-13 0.1088594831012486 1106 14-15 0.014883093785414303 1106 16-17 0.2190801297789804 1106 18-19 0.17175414937105415 1106 20-21 0.15828796828451175 1106 22-23 0.1803917703148059 1106 24-25 0.1487773972812505 1106 26-27 0.22680951693929785 1106 28-29 0.26946311264492095 1106 30-31 0.28308121513939 1106 32-33 0.23434966313814698 1106 34-35 0.24313996085498246 1106 36-37 0.15066697392401807 1106 38-39 0.3181910479194272 1106 40-41 0.2605676412818454 1106 42-43 0.2416355684927609 1106 44-45 0.24427662094108626 1106 46-47 0.21243124148833914 1106 48-49 0.16655346986393482 1106 50-51 0.30407628828307054 1106 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-0.6895162248379876 2216 86-87 -0.8154860506807289 2216 88-89 -0.9760035492664407 2216 90-91 -0.7984903728084021 2216 92-93 -0.7323302053371705 2216 94-95 -0.8728469615716605 2216 96-97 -0.822443098140063 2216 98-99 -0.8311727437452632 2216 100-101 -0.923375199485676 2216 102-103 -1.022086453914259 2216 104-105 -1.0628820857295445 2216 106-107 -1.0237846373126445 2216 108-109 -0.8405799139133698 2216 110-111 -0.8260125530972644 2216 112-113 -1.0332024283801324 2216 114-115 -0.9817358304481196 2216 116-117 -1.1225197845084978 2216 118-119 -1.0055288521183172 2216 120-121 -1.0781245083685143 2216 122-123 -1.227968565048183 2216 124-125 -1.3454432479761032 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 2.0 4 9.0 5 43.0 6 98.0 7 214.0 8 329.0 9 600.0 10 1082.0 11 1593.0 12 2094.0 13 3342.0 14 6382.0 15 6645.0 16 2745.0 17 2034.0 18 2011.0 19 1723.0 20 1466.0 21 1399.0 22 1384.0 23 1466.0 24 1646.0 25 1970.0 26 2457.0 27 2883.0 28 3587.0 29 4286.0 30 5143.0 31 6197.0 32 7995.0 33 10501.0 34 15908.0 35 31069.0 36 76980.0 37 219115.0 38 117877.0 39 591.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.78924085369771 17.816451688103335 13.928848734266671 22.46545872393228 2 16.23600320818123 19.53980585731669 40.090519163743885 24.1336717707582 3 19.216729158751917 25.19669865600722 28.91025182199758 26.676320363243278 4 12.760306578197659 16.317753230282868 36.024592423769 34.897347767750475 5 13.774039432564733 37.33754907037123 32.635423207852725 16.252988289211313 6 32.02782698079094 36.15855505281803 16.43168531282409 15.381932653566938 7 28.80314790106282 33.25961557873332 19.72411818577241 18.213118334431453 8 27.386115245626947 34.17304279361537 18.56164119580598 19.879200764951705 9 27.924407795606665 15.50660615081368 19.260338685670554 37.3086473679091 10-11 25.914023686340435 26.380923190993833 25.48658306278 22.218470059885735 12-13 27.11623485191908 23.790309999981645 26.902147480779853 22.19130766731942 14-15 23.98448440089489 24.243444778672384 25.975506128973645 25.79656469145908 16-17 22.32638551143298 27.757304022419987 26.507558669850294 23.408751796296738 18-19 22.26371012588301 24.921036355394214 30.70001890355699 22.11523461516579 20-21 23.078123078673666 23.95612906541181 31.28584564052776 21.67990221538676 22-23 22.873212191509552 23.891159558868065 31.24721264299256 21.988415606629825 24-25 22.563872916708725 24.201141187547144 31.165541935614115 22.069443960130013 26-27 22.203053566807558 24.448722725195505 31.19362197657794 22.154601731418996 28-29 22.120801402904732 24.377855614968354 31.28372940676621 22.217613575360705 30-31 22.344942513863387 24.103158456917743 31.247516611745358 22.30438241747351 32-33 22.19057354860141 24.31749900435149 31.073501801343806 22.418425645703294 34-35 22.596544291124523 24.368905043712545 30.86526699659458 22.169283668568358 36-37 22.843307112626686 24.111242846812587 30.616376256740242 22.429073783820492 38-39 22.56588238856867 24.2132059351734 30.699261361081465 22.521650315176466 40-41 22.595990610621598 24.02944917237291 30.82729673629171 22.547263480713784 42-43 22.527701943150795 24.230792172337058 30.349583610543945 22.891922273968206 44-45 22.623464408975828 24.414279906802346 30.055417549533047 22.90683813468878 46-47 23.059607741656045 24.884536784428594 29.09948327171319 22.956372202202175 48-49 23.326784208319072 24.94776147481034 28.670943633782475 23.054510683088118 50-51 23.256622777877702 25.245107935587356 28.314692527649203 23.183576758885735 52-53 23.39170500146365 25.322897826180718 28.031614851602356 23.25378232075328 54-55 23.284593968113555 25.190090865544324 27.968271389007672 23.557043777334453 56-57 23.194003580746195 25.385122926411167 28.079951033158153 23.34092245968449 58-59 23.299394795887 24.932849414755236 28.266565111061148 23.50119067829662 60-61 23.29877116011625 25.096836180070785 28.497045590317576 23.107347069495386 62-63 23.13577262854201 25.07293017331641 28.75270362407214 23.038593574069434 64-65 23.036418596370805 25.003074189836273 28.627131055924558 23.333376157868365 66-67 23.281158377617963 25.287983915296525 28.230453203588773 23.200404503496735 68-69 23.118973244601385 25.069467131126782 28.622935715832632 23.1886239084392 70-71 23.23529870599431 25.165983461761172 28.329648890491754 23.269068941752767 72-73 23.15878437281485 25.403994707004042 27.916516019388077 23.52070490079303 74-75 23.053939471800614 25.634187971442728 27.852148218841194 23.459724337915468 76-77 23.282550980422748 25.44428058435343 27.908575246195454 23.36459318902837 78-79 23.0357060923348 25.36086920613363 28.031438980297875 23.571985721233702 80-81 23.13621022223609 25.763804643667953 27.733353399244958 23.366631734851 82-83 23.115104309093347 25.386559387451342 27.807682919443316 23.690653384011995 84-85 23.066278316439366 25.3141580820344 27.5514848523223 24.068078749203934 86-87 23.053960627269465 25.390299879145374 27.711406125686977 23.844333367898184 88-89 22.992139423826924 25.55540669259329 27.58542847756625 23.867025406013536 90-91 23.109570273248917 25.54932878423182 27.659191788125415 23.68190915439385 92-93 22.892721624982222 25.199888046176365 27.68562999261288 24.22176033622853 94-95 22.91589429984106 25.12296691639351 27.824843721896553 24.136295061868875 96-97 23.083939596379306 24.94191253661437 27.86959410352599 24.10455376348033 98-99 22.80925209820306 25.235697990900597 27.629292070967253 24.325757839929086 100-101 22.824702496751506 25.021564856075234 27.85940815023088 24.29432449694238 102-103 22.867522771444985 24.627847692389576 27.944779590031406 24.559849946134037 104-105 22.710329600951418 24.955631699980362 27.71793690616678 24.61610179290144 106-107 22.8845125016403 24.845352594249807 27.73329711664374 24.53683778746615 108-109 22.922996592737107 24.799698274161237 27.50935781399263 24.767947319109027 110-111 22.630218895848742 25.161001411343236 27.53385663762615 24.674923055181868 112-113 22.501280124213345 24.967193949371318 27.85430993504861 24.677215991366733 114-115 22.41123310097751 25.209138594926344 27.385042598567686 24.994585705528454 116-117 22.2666265345488 24.93268999590726 27.949756546092903 24.850926923451045 118-119 22.198016585653235 25.09192584999867 27.529670002945668 25.18038756140243 120-121 22.252096830436617 25.201974782975757 27.63897810486905 24.906950281718576 122-123 22.164766814653852 25.246711582826638 27.362457260212125 25.226064342307385 124-125 22.243277444717805 25.24907614475919 27.276739954135305 25.230906456387704 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 321.0 1 234.5 2 338.0 3 523.0 4 313.0 5 179.0 6 504.5 7 1426.0 8 2625.5 9 2870.0 10 1879.5 11 950.5 12 718.0 13 729.0 14 783.5 15 831.5 16 879.5 17 930.5 18 999.0 19 1078.0 20 1091.5 21 1144.5 22 1299.0 23 1462.0 24 1629.0 25 1931.0 26 2299.5 27 2645.0 28 2990.0 29 3539.0 30 4404.5 31 5229.5 32 6430.0 33 7296.5 34 7750.5 35 8387.5 36 9422.0 37 10727.5 38 11554.5 39 12143.0 40 12497.0 41 13115.5 42 13740.5 43 14835.0 44 16170.0 45 17120.0 46 17948.0 47 18736.5 48 19664.0 49 20103.5 50 20454.5 51 20794.0 52 21258.0 53 22046.5 54 22510.5 55 21446.5 56 19604.5 57 18114.0 58 16916.5 59 15122.5 60 12764.0 61 10973.5 62 9101.5 63 7068.0 64 5458.0 65 4142.5 66 2959.0 67 2271.5 68 1609.5 69 998.0 70 787.5 71 622.0 72 471.0 73 392.5 74 282.0 75 191.0 76 121.5 77 60.0 78 29.0 79 14.0 80 11.0 81 8.0 82 4.5 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01615061179618662 2 0.0025694155130296897 3 3.6705935900424136E-4 4 0.14700727328119867 5 0.04496477147801957 6 0.013948255642161171 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.7529451925318107E-4 30-31 9.176483975106034E-5 32-33 0.0 34-35 8.258835577595431E-4 36-37 0.0016517671155190862 38-39 0.003211769391287112 40-41 0.0 42-43 0.0015600022757680258 44-45 0.0013764725962659053 46-47 9.176483975106034E-5 48-49 0.00266118035278075 50-51 0.0016517671155190862 52-53 9.176483975106034E-5 54-55 0.0 56-57 0.0022941209937765083 58-59 0.0024776506732786295 60-61 0.0013764725962659053 62-63 9.176483975106034E-5 64-65 0.0022023561540254486 66-67 0.0010094132372616637 68-69 0.0016517671155190862 70-71 0.0022941209937765083 72-73 0.0 74-75 1.8352967950212068E-4 76-77 0.00541412554531256 78-79 0.0011011780770127243 80-81 0.0 82-83 0.0 84-85 3.6705935900424136E-4 86-87 2.7529451925318107E-4 88-89 0.0 90-91 2.7529451925318107E-4 92-93 6.423538782574224E-4 94-95 0.0016517671155190862 96-97 5.505890385063621E-4 98-99 0.0 100-101 5.505890385063621E-4 102-103 0.0 104-105 0.0 106-107 4.588241987553017E-4 108-109 0.0010094132372616637 110-111 0.0 112-113 3.6705935900424136E-4 114-115 0.0031200045515360516 116-117 7.341187180084827E-4 118-119 4.588241987553017E-4 120-121 1.8352967950212068E-4 122-123 7.341187180084827E-4 124-125 0.0013764725962659053 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 544871.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.43863771772378 #Duplication Level Percentage of deduplicated Percentage of total 1 70.70409115146845 35.66218038749836 2 14.963626598322307 15.094898818721486 3 5.804676758228753 8.783399642303742 4 2.83110970788418 5.711892675804037 5 1.550628173070372 3.910578632819619 6 1.0120710151456214 3.062848996652335 7 0.6418589525484724 2.266214382142904 8 0.4535970015996236 1.830305186682335 9 0.3309390177301643 1.5022901899756012 >10 1.5976973103868457 14.03112655566331 >50 0.07555793831948232 2.6343810267843297 >100 0.03160356011419181 2.7631867896442266 >500 0.0010897779349721304 0.3701105573291781 >1k 0.0010897779349721304 1.3981750806826525 >5k 3.632593116573768E-4 0.9784110772959461 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5340 0.9800484885413244 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3949 0.7247587043538746 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2591 0.4755253995899947 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1091 0.20023088033681366 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 791 0.14517197648617747 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 641 0.11764252456085936 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 588 0.10791545154724695 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 9.176483975106034E-5 0.0 22-23 0.0 0.0 0.0 5.50589038506362E-4 0.0 24-25 0.0 0.0 0.0 0.0013764725962659053 0.0 26-27 0.0 0.0 0.0 0.002110591314274388 0.0 28-29 0.0 0.0 0.0 0.004129417788797715 0.0 30-31 0.0 0.0 0.0 0.009360013654608155 0.0 32-33 0.0 0.0 0.0 0.017802378911705705 0.0 34-35 0.0 0.0 0.0 0.027162392566313864 0.0 36-37 0.0 0.0 0.0 0.04780948151030244 0.0 38-39 0.0 0.0 0.0 0.07900952702566295 0.0 40-41 0.0 0.0 0.0 0.11516487388758073 0.0 42-43 0.0 0.0 0.0 0.15003551299298368 0.0 44-45 0.0 0.0 0.0 0.18619085985490144 0.0 46-47 0.0 0.0 0.0 0.23381681168570176 0.0 48-49 0.0 0.0 0.0 0.2880498319785784 0.0 50-51 0.0 0.0 0.0 0.3529275736825781 0.0 52-53 0.0 0.0 0.0 0.41184060080275886 0.0 54-55 0.0 0.0 0.0 0.469835979525429 0.0 56-57 0.0 0.0 0.0 0.5404031412939944 0.0 58-59 0.0 0.0 0.0 0.626478560980489 0.0 60-61 0.0 0.0 0.0 0.7155822203787685 0.0 62-63 0.0 0.0 0.0 0.8095494162838544 0.0 64-65 0.0 0.0 0.0 0.8947989524125894 0.0 66-67 0.0 0.0 0.0 1.0000532236070556 0.0 68-69 0.0 0.0 0.0 1.1341216544833546 0.0 70-71 0.0 0.0 0.0 1.2844324619955916 0.0 72-73 0.0 0.0 0.0 1.4350185640270818 0.0 74-75 0.0 0.0 0.0 1.5750517094871996 0.0 76-77 0.0 0.0 0.0 1.7294919347882343 0.0 78-79 0.0 0.0 0.0 1.9109110229760806 0.0 80-81 0.0 0.0 0.0 2.114996026582439 0.0 82-83 0.0 0.0 0.0 2.3279822196446496 0.0 84-85 0.0 0.0 0.0 2.5480343053676924 0.0 86-87 0.0 0.0 0.0 2.7537710760895697 0.0 88-89 0.0 0.0 0.0 3.00300438085345 0.0 90-91 0.0 0.0 0.0 3.2910542128320284 0.0 92-93 0.0 0.0 0.0 3.6014946657098657 0.0 94-95 0.0 0.0 0.0 3.8982621574647944 0.0 96-97 0.0 0.0 0.0 4.207601432265619 0.0 98-99 0.0 0.0 0.0 4.53566073437566 0.0 100-101 0.0 0.0 0.0 4.9366730840877935 0.0 102-103 0.0 0.0 0.0 5.357690168865658 0.0 104-105 0.0 0.0 0.0 5.774394306175223 0.0 106-107 0.0 0.0 0.0 6.170267824861297 0.0 108-109 0.0 0.0 0.0 6.59798374294099 0.0 110-111 0.0 0.0 0.0 7.081951507788082 0.0 112-113 0.0 0.0 0.0 7.602716973375349 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1765 0.0 72.92639 1 TATAACG 45 3.954641E-6 66.199295 2 GTATCAA 3610 0.0 58.599888 1 TATCAAC 4550 0.0 46.22311 2 ATCAACG 4540 0.0 46.18944 3 TCAACGC 4545 0.0 46.13863 4 CAACGCA 4630 0.0 45.37856 5 GTGGTAT 685 0.0 45.236446 1 AACGCAG 4805 0.0 43.95745 6 ACGCAGA 5520 0.0 38.36795 7 CGCAGAG 5555 0.0 38.233303 8 TGGTATC 845 0.0 35.25406 2 TAACGCA 85 1.6968789E-4 35.04346 4 GCAGAGT 6080 0.0 34.344814 9 GTACATG 3410 0.0 32.853313 1 GTATAGG 165 9.264113E-9 32.503807 1 TACATGG 3425 0.0 31.833645 2 GTATAAC 150 1.4563557E-7 31.781498 1 GAGTACT 4100 0.0 30.761654 12-13 ACATGGG 3425 0.0 30.613152 3 >>END_MODULE