FastQCFastQC Report
Fri 27 May 2016
SRR940036_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940036_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868241
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11250.059.3824041
GTATCAA18950.052.565351
ATCAACG22700.041.483213
CAACGCA22850.040.6681065
TCAACGC23100.040.511564
AACGCAG24400.038.0846826
TATCAAC25950.037.227992
GTACATG50500.034.4892461
TACATGG51550.033.6632652
ACATGGG52700.031.5525653
ACGCAGA29700.031.0878587
CGCAGAG29800.030.5837488
CATGGGG27200.028.7073884
TATACCG850.00716911628.0321665
GTGGTAT5850.027.5296151
GTGTAGG5250.027.2674311
CATGGGA26400.027.0937164
GCAGAGT34650.025.4403979
GTATACC1701.3802983E-524.5408863
GTATAGG2752.055458E-923.8590011
Produced by FastQC (version 0.11.5)