##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940036_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 868241 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.09122351973703 33.0 31.0 34.0 28.0 34.0 2 31.133540111558887 34.0 31.0 34.0 28.0 34.0 3 31.36115203036945 34.0 31.0 34.0 28.0 34.0 4 34.57461119666083 37.0 35.0 37.0 32.0 37.0 5 34.69743769299077 37.0 35.0 37.0 32.0 37.0 6 34.65185242346307 37.0 35.0 37.0 32.0 37.0 7 34.61669628593904 37.0 35.0 37.0 32.0 37.0 8 34.58488944889725 37.0 35.0 37.0 32.0 37.0 9 36.171393656830304 39.0 38.0 39.0 32.0 39.0 10-11 36.205178055401674 39.0 38.0 39.0 32.0 39.0 12-13 36.145745824028126 39.0 38.0 39.0 32.0 39.0 14-15 37.559776605804146 41.0 39.0 41.0 31.5 41.0 16-17 37.582803046619546 41.0 39.0 41.0 32.0 41.0 18-19 37.58861019002788 41.0 39.0 41.0 32.0 41.0 20-21 37.611986188166654 41.0 39.0 41.0 32.0 41.0 22-23 37.60513497980399 41.0 39.0 41.0 31.0 41.0 24-25 37.67661858861768 41.0 39.0 41.0 31.5 41.0 26-27 37.72401787061426 41.0 39.0 41.0 31.0 41.0 28-29 37.67248321606559 41.0 39.0 41.0 31.0 41.0 30-31 37.614433089430236 41.0 38.0 41.0 31.0 41.0 32-33 37.57599099789114 41.0 38.0 41.0 31.0 41.0 34-35 37.484482994928825 41.0 38.0 41.0 30.0 41.0 36-37 37.33198731688552 40.5 38.0 41.0 30.0 41.0 38-39 37.22133140452939 40.0 38.0 41.0 30.0 41.0 40-41 37.12141732537395 40.0 38.0 41.0 30.0 41.0 42-43 36.92102999052106 40.0 38.0 41.0 30.0 41.0 44-45 36.71954964117106 40.0 37.0 41.0 30.0 41.0 46-47 36.53480773195461 40.0 37.0 41.0 29.5 41.0 48-49 36.3650668420404 40.0 36.5 41.0 29.0 41.0 50-51 36.17980837117805 40.0 36.0 41.0 29.0 41.0 52-53 36.32462588152368 40.0 36.0 41.0 29.5 41.0 54-55 36.1345150712763 40.0 36.0 41.0 29.0 41.0 56-57 36.00091391675814 39.5 35.0 41.0 29.0 41.0 58-59 35.79227138547938 39.0 35.0 41.0 29.0 41.0 60-61 35.533804554265465 39.0 35.0 41.0 28.5 41.0 62-63 35.23256042965029 38.5 35.0 41.0 28.0 41.0 64-65 34.91333397063718 37.5 35.0 40.0 28.0 41.0 66-67 34.586752986785925 37.0 34.5 40.0 28.0 41.0 68-69 34.23609516251824 36.5 34.0 39.5 27.0 41.0 70-71 33.87400042154195 36.0 34.0 39.0 26.5 41.0 72-73 33.50968970596873 35.5 34.0 39.0 26.0 40.5 74-75 33.13881226525815 35.0 34.0 37.5 26.0 40.0 76-77 32.7849128295024 35.0 34.0 37.0 26.0 39.0 78-79 32.45576631373086 35.0 33.0 37.0 25.5 39.0 80-81 32.14791745609802 35.0 33.0 36.0 25.0 38.5 82-83 31.84699467083448 35.0 33.0 36.0 24.0 37.0 84-85 31.61168730801701 35.0 33.0 35.0 24.0 37.0 86-87 31.40339778932347 35.0 33.0 35.0 23.0 36.5 88-89 31.227977600689208 35.0 33.0 35.0 22.5 36.0 90-91 31.076876696677537 35.0 33.0 35.0 20.5 36.0 92-93 30.923708970205276 35.0 32.5 35.0 20.0 36.0 94-95 30.80792256988555 35.0 32.0 35.0 19.5 35.0 96-97 30.698552014935945 35.0 32.0 35.0 19.0 35.0 98-99 30.62376978281376 35.0 32.0 35.0 18.5 35.0 100-101 30.519591334663993 35.0 32.0 35.0 18.0 35.0 102-103 29.78835657380842 33.5 30.0 34.5 18.5 35.0 104-105 30.75225772567755 35.0 33.0 35.0 19.0 35.0 106-107 30.88028899810076 35.0 33.0 35.0 19.0 35.0 108-109 30.863612176803446 35.0 33.0 35.0 19.0 35.0 110-111 30.812476604997922 35.0 33.0 35.0 18.0 35.0 112-113 30.748690743699044 35.0 33.0 35.0 17.5 35.0 114-115 30.67478902747048 35.0 33.0 35.0 16.0 35.0 116-117 30.587516023776807 35.0 33.0 35.0 8.5 35.0 118-119 30.495896300681494 35.0 33.0 35.0 3.5 35.0 120-121 30.38639847692058 35.0 33.0 35.0 2.0 35.0 122-123 30.22531762494515 35.0 33.0 35.0 2.0 35.0 124-125 28.885881339397702 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2715711007258754 1101 2 0.4161158396980831 1101 3 0.3735326076852665 1101 4 0.28230731501726325 1101 5 0.467530573770496 1101 6 0.4880109316584864 1101 7 0.5527202342629778 1101 8 0.5658568457024202 1101 9 0.655366390599255 1101 10-11 0.5141250729231288 1101 12-13 0.5633150146124066 1101 14-15 0.6620853795475128 1101 16-17 0.6606031958540157 1101 18-19 0.5626093129352654 1101 20-21 0.4771788554678835 1101 22-23 0.5250690061526484 1101 24-25 0.5999536760560389 1101 26-27 0.5383438917920245 1101 28-29 0.6129290095066793 1101 30-31 0.6597505017529315 1101 32-33 0.7007855278326289 1101 34-35 0.7139665079620485 1101 36-37 0.809592016323748 1101 38-39 0.6883014604019309 1101 40-41 0.6949427921147233 1101 42-43 0.735605925345169 1101 44-45 0.7864801601170015 1101 46-47 0.7090697483409798 1101 48-49 0.7337280557632013 1101 50-51 0.7023174654892372 1101 52-53 0.6995628205549025 1101 54-55 0.6464479425142713 1101 56-57 0.7553836443868889 1101 58-59 0.8039705693851147 1101 60-61 0.7148001580523768 1101 62-63 0.7210515822751447 1101 64-65 0.7894894510426411 1101 66-67 0.8670509944405183 1101 68-69 0.8831159561865718 1101 70-71 1.0019232664517332 1101 72-73 1.0425145607909343 1101 74-75 0.9092747718673593 1101 76-77 0.8199436538287301 1101 78-79 0.7204874606113094 1101 80-81 0.6783837984042975 1101 82-83 0.7596116469316279 1101 84-85 0.5634324584485206 1101 86-87 0.5979742042862739 1101 88-89 0.7395714861458025 1101 90-91 0.730863819666812 1101 92-93 0.8331339811644654 1101 94-95 0.7781062891072317 1101 96-97 0.83206094789589 1101 98-99 0.7936670226891529 1101 100-101 0.6796820934810519 1101 102-103 0.6664844099949327 1101 104-105 0.6638913774863369 1101 106-107 0.7220141678710199 1101 108-109 0.7364050250818792 1101 110-111 0.769515514166919 1101 112-113 0.7047520334276491 1101 114-115 0.6719422652412241 1101 116-117 0.6058856949621685 1101 118-119 0.7130563250624782 1101 120-121 0.6657690909490839 1101 122-123 0.5895678140235852 1101 124-125 0.6552071179260324 1102 1 0.03415476302837561 1102 2 0.11239446474896653 1102 3 0.2690008799108021 1102 4 0.31177727232226715 1102 5 0.3551130527306654 1102 6 0.33933580172749345 1102 7 0.29300026039117455 1102 8 0.45715028619923004 1102 9 0.3566055866838127 1102 10-11 0.4103506842377769 1102 12-13 0.2508605732919804 1102 14-15 0.32569428964189484 1102 16-17 0.30285514363548316 1102 18-19 0.29985524933771757 1102 20-21 0.32074099987351445 1102 22-23 0.35037927851072936 1102 24-25 0.47453832289935605 1102 26-27 0.4196499230511961 1102 28-29 0.40166453723001894 1102 30-31 0.3829809316375332 1102 32-33 0.32381052948930744 1102 34-35 0.26099726539580814 1102 36-37 0.36270515331692366 1102 38-39 0.34726220183689804 1102 40-41 0.37548296422003347 1102 42-43 0.37328543837613637 1102 44-45 0.2049384070663436 1102 46-47 0.13938035203490529 1102 48-49 0.16829395846406214 1102 50-51 0.2170193105566014 1102 52-53 0.14736795720727258 1102 54-55 0.32453622931858916 1102 56-57 0.30017551813845955 1102 58-59 0.32665287510396723 1102 60-61 0.3264035395714089 1102 62-63 0.2736961157069331 1102 64-65 0.2593236884495127 1102 66-67 0.31370592235130346 1102 68-69 0.3281710052716136 1102 70-71 0.4161262143941329 1102 72-73 0.4449266857897527 1102 74-75 0.2807732619225476 1102 76-77 0.21495752260079826 1102 78-79 0.21939878567071247 1102 80-81 0.17132634476764963 1102 82-83 0.3336510096307954 1102 84-85 0.28480267419548966 1102 86-87 0.3558860310828216 1102 88-89 0.36567791122129734 1102 90-91 0.26361400190153006 1102 92-93 0.21376314061274115 1102 94-95 0.22774323968139143 1102 96-97 0.14699094136383906 1102 98-99 0.14431975431658017 1102 100-101 0.2703308490782632 1102 102-103 0.3474647854457906 1102 104-105 0.3223835153599204 1102 106-107 0.298391815007804 1102 108-109 0.27444238083245054 1102 110-111 0.34114085851953035 1102 112-113 0.346559044494267 1102 114-115 0.1753171575570711 1102 116-117 0.15523580335632303 1102 118-119 0.1813048837515261 1102 120-121 0.3520461066544023 1102 122-123 0.3136833839685842 1102 124-125 0.19326340715382884 1103 1 -0.0395816640351363 1103 2 0.07403523988552507 1103 3 0.15516334807889365 1103 4 0.10423796169017407 1103 5 -0.05449379361469653 1103 6 0.10469321844386315 1103 7 0.21438846294151404 1103 8 0.13942760484018635 1103 9 0.25705336342024765 1103 10-11 0.14111663805902452 1103 12-13 0.17109383373142606 1103 14-15 0.31672455480524775 1103 16-17 0.3302376850761348 1103 18-19 0.26270303364754 1103 20-21 0.26865027065063884 1103 22-23 0.16752448881432258 1103 24-25 0.1824279028601623 1103 26-27 0.23281436976765946 1103 28-29 0.31536574802589 1103 30-31 0.2792444099066316 1103 32-33 0.2607667836901726 1103 34-35 0.24067691096110622 1103 36-37 0.3648872365674265 1103 38-39 0.3124814041695032 1103 40-41 0.27788562248023396 1103 42-43 0.2923069562730092 1103 44-45 0.19510246564652078 1103 46-47 0.08207818320508409 1103 48-49 0.1920223387997524 1103 50-51 0.1835733230337624 1103 52-53 0.18597331727468003 1103 54-55 0.4238612508554169 1103 56-57 0.19915121702043592 1103 58-59 0.2383660707909243 1103 60-61 0.1388863811076746 1103 62-63 0.14748082313737143 1103 64-65 0.07440041636598238 1103 66-67 0.1285317816945053 1103 68-69 0.04665859911065695 1103 70-71 0.007546509188379957 1103 72-73 0.0247076254582268 1103 74-75 -0.01387424406516402 1103 76-77 -0.11023441552459445 1103 78-79 -0.08597926853583004 1103 80-81 -0.19743625782813012 1103 82-83 0.027652884045036075 1103 84-85 -0.02747663246057286 1103 86-87 0.09844280784766113 1103 88-89 0.002458084083944101 1103 90-91 -0.10231331247939224 1103 92-93 -0.017381482753059174 1103 94-95 -0.14551133038667885 1103 96-97 -0.01189406616222044 1103 98-99 -0.07840420505217693 1103 100-101 -0.07711265815905932 1103 102-103 -0.07062899206691853 1103 104-105 -0.0024628868060716513 1103 106-107 -0.05024450129486624 1103 108-109 -0.21204858316554365 1103 110-111 -0.16862881572998845 1103 112-113 -0.20152725429493756 1103 114-115 -0.24042886877939296 1103 116-117 -0.09748825067982025 1103 118-119 -0.07372905450640488 1103 120-121 -0.14585227737331508 1103 122-123 -0.12927004914417495 1103 124-125 -0.17468978929046486 1104 1 0.15869769812267265 1104 2 0.19237709379624945 1104 3 0.27559869262939074 1104 4 0.23967538704349067 1104 5 0.2569000397654406 1104 6 0.3504495648924575 1104 7 0.14645025094323216 1104 8 0.2758732891198079 1104 9 0.38172136540969603 1104 10-11 0.46057146772078283 1104 12-13 0.4677587974104185 1104 14-15 0.61250686899632 1104 16-17 0.5442554446435963 1104 18-19 0.4784196925887372 1104 20-21 0.49712495759977315 1104 22-23 0.4749491485269317 1104 24-25 0.5947571670679892 1104 26-27 0.6212300498856678 1104 28-29 0.6095548946937299 1104 30-31 0.6092711558355361 1104 32-33 0.7435096539800341 1104 34-35 0.8688268742510061 1104 36-37 0.7064146473381996 1104 38-39 0.7492195004548421 1104 40-41 0.6653619808384619 1104 42-43 0.6303282988814161 1104 44-45 0.6564646640569904 1104 46-47 0.5700370171434912 1104 48-49 0.6324576664331545 1104 50-51 0.6270558425983737 1104 52-53 0.5834671340760664 1104 54-55 0.7788512866858355 1104 56-57 0.675814082979521 1104 58-59 0.6818726972174645 1104 60-61 0.6167110286399762 1104 62-63 0.802710827975659 1104 64-65 0.8069914557185314 1104 66-67 0.8658693352793989 1104 68-69 0.7131849948556663 1104 70-71 0.7548871416847689 1104 72-73 0.7181093658890774 1104 74-75 0.6540867214273476 1104 76-77 0.607992317547307 1104 78-79 0.5609203352657772 1104 80-81 0.45781404752545996 1104 82-83 0.7326090010978099 1104 84-85 0.5058331297188907 1104 86-87 0.4560520330784925 1104 88-89 0.5939169806155995 1104 90-91 0.45357316027224215 1104 92-93 0.4534043840281896 1104 94-95 0.47400964512075205 1104 96-97 0.4914106885568046 1104 98-99 0.6280858696875526 1104 100-101 0.531744625834996 1104 102-103 0.47613705126114425 1104 104-105 0.5429328717395983 1104 106-107 0.5507467559498771 1104 108-109 0.5019256148795144 1104 110-111 0.5360071452794877 1104 112-113 0.5605545127972498 1104 114-115 0.6177162054697405 1104 116-117 0.5554029656255537 1104 118-119 0.5077039250340576 1104 120-121 0.552382254479884 1104 122-123 0.610840216488068 1104 124-125 0.4764729227230404 1105 1 -0.06429107114684385 1105 2 0.08763228776691534 1105 3 0.10724590948775514 1105 4 0.025750637458429537 1105 5 -0.09144473526475139 1105 6 0.011499789959138695 1105 7 -0.08761102402560539 1105 8 0.00338922461575919 1105 9 0.04933791895102502 1105 10-11 -0.1058345047803968 1105 12-13 -0.009172675113354956 1105 14-15 -0.05871575650176197 1105 16-17 0.03184367917793196 1105 18-19 -0.0947576547889355 1105 20-21 -0.14175926706649733 1105 22-23 -0.1296753743769088 1105 24-25 -0.20169875925738978 1105 26-27 -0.07212709251017202 1105 28-29 -0.012494536019964642 1105 30-31 0.005151983512597269 1105 32-33 -1.33907562954505E-4 1105 34-35 0.040362720236942096 1105 36-37 0.11103739424959258 1105 38-39 0.03734279308027766 1105 40-41 0.09419554128356111 1105 42-43 0.0900164991875343 1105 44-45 0.08729137240027995 1105 46-47 -0.13708325952384826 1105 48-49 -0.034817412194875885 1105 50-51 -0.17292542875389216 1105 52-53 0.06960874202734146 1105 54-55 0.1347730913524927 1105 56-57 0.015433891430653546 1105 58-59 -0.07736165628899272 1105 60-61 0.021896442252476334 1105 62-63 -0.09640553505010985 1105 64-65 -0.06121295132602711 1105 66-67 0.04603408279785981 1105 68-69 -0.08787882462600294 1105 70-71 -0.08130500132460838 1105 72-73 -0.08699564370128599 1105 74-75 -0.10832342949971974 1105 76-77 -0.0851557421002056 1105 78-79 -0.11287828656407584 1105 80-81 -0.13705220409715224 1105 82-83 -0.10353389848435057 1105 84-85 -0.05294091883351726 1105 86-87 -0.20686540587580282 1105 88-89 -0.15867112475564582 1105 90-91 -0.21041068142547914 1105 92-93 -0.17155121616038826 1105 94-95 -0.20783099346155254 1105 96-97 -0.23002650912084022 1105 98-99 -0.11317140380882762 1105 100-101 -0.11735639907410444 1105 102-103 -0.23569606357129658 1105 104-105 -0.25497775386181587 1105 106-107 -0.16577497896871662 1105 108-109 -0.20319667027383304 1105 110-111 -0.2015366894434294 1105 112-113 -0.34207185713875177 1105 114-115 -0.3474129899088041 1105 116-117 -0.2611717261590272 1105 118-119 -0.3438408654957037 1105 120-121 -0.3486788202861675 1105 122-123 -0.2790224185680863 1105 124-125 -0.2667879931724144 1106 1 0.1448869167271205 1106 2 0.3241319326273384 1106 3 0.2766941142843926 1106 4 0.26042096379828195 1106 5 0.25721917835784325 1106 6 0.39843640415011095 1106 7 0.493951403605962 1106 8 0.3461253380366145 1106 9 0.5371512694015195 1106 10-11 0.559189997519077 1106 12-13 0.5032059207719968 1106 14-15 0.6035705963468914 1106 16-17 0.6277321420374804 1106 18-19 0.5209976423859644 1106 20-21 0.40244900481032886 1106 22-23 0.6785356546885879 1106 24-25 0.6181001555852674 1106 26-27 0.6652829375018428 1106 28-29 0.6315440925972879 1106 30-31 0.6519796623296514 1106 32-33 0.6708840607899944 1106 34-35 0.7228593903177156 1106 36-37 0.6892846677567945 1106 38-39 0.7214383683675365 1106 40-41 0.7755483044559739 1106 42-43 0.7554847316803972 1106 44-45 0.7798411346821226 1106 46-47 0.8653801813046158 1106 48-49 0.797480939242547 1106 50-51 0.6475771598017559 1106 52-53 0.6341423435156699 1106 54-55 0.8279029667505711 1106 56-57 0.5900089284599801 1106 58-59 0.5296196728500107 1106 60-61 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8 -0.43374854077220704 2216 9 -0.9047381819849676 2216 10-11 -0.6959819659956281 2216 12-13 -0.7220892176176577 2216 14-15 -0.7747024663559046 2216 16-17 -0.9124249696863629 2216 18-19 -1.049904868488305 2216 20-21 -0.9570440025205755 2216 22-23 -0.9899509711570715 2216 24-25 -1.0668938551430998 2216 26-27 -1.1158760065949878 2216 28-29 -1.1953381438500017 2216 30-31 -1.2905035307040365 2216 32-33 -1.1793999405242985 2216 34-35 -1.4709099286778198 2216 36-37 -1.498921770486291 2216 38-39 -1.3587808658448068 2216 40-41 -1.414241837688465 2216 42-43 -1.296037146721119 2216 44-45 -1.3334576882527571 2216 46-47 -1.3233132240853962 2216 48-49 -1.3282016043758986 2216 50-51 -1.3396348849275697 2216 52-53 -1.5242570383707559 2216 54-55 -1.4258896493566837 2216 56-57 -1.632200827570891 2216 58-59 -1.5191932445546072 2216 60-61 -1.4481012378929705 2216 62-63 -1.4168165181540004 2216 64-65 -1.4372900093233625 2216 66-67 -1.478309830301754 2216 68-69 -1.439184640666383 2216 70-71 -1.6111828352599034 2216 72-73 -1.4967001582935602 2216 74-75 -1.5259346199345458 2216 76-77 -1.4932315052498453 2216 78-79 -1.2879967301843323 2216 80-81 -1.4648362479627863 2216 82-83 -1.7458882790783292 2216 84-85 -1.5228645868827115 2216 86-87 -1.515279877497381 2216 88-89 -1.3995297815501502 2216 90-91 -1.4791692292159375 2216 92-93 -1.5468541804297757 2216 94-95 -1.4981231786577744 2216 96-97 -1.6368632369805454 2216 98-99 -1.684629233415027 2216 100-101 -1.6549501760399856 2216 102-103 -1.61798261511235 2216 104-105 -1.6986351356098979 2216 106-107 -1.6809355682311846 2216 108-109 -1.6867686541959763 2216 110-111 -1.6008292794008838 2216 112-113 -1.6334317578217963 2216 114-115 -1.5225775394250824 2216 116-117 -1.5781521879362685 2216 118-119 -1.614102950821703 2216 120-121 -1.7226539347501237 2216 122-123 -1.9024165987421604 2216 124-125 -1.8256047994024343 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20590.0 3 3256.0 4 2107.0 5 1642.0 6 1699.0 7 1882.0 8 2085.0 9 2334.0 10 2508.0 11 2889.0 12 3246.0 13 3260.0 14 3266.0 15 3238.0 16 3198.0 17 3077.0 18 2630.0 19 2307.0 20 2157.0 21 2007.0 22 2132.0 23 2733.0 24 4568.0 25 7764.0 26 8590.0 27 7261.0 28 7929.0 29 9152.0 30 10687.0 31 12691.0 32 16850.0 33 22661.0 34 35238.0 35 62902.0 36 149715.0 37 304398.0 38 133122.0 39 470.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.19917769829271 18.41271341126148 13.489965522327518 23.898143368118294 2 15.450812153045012 19.52940470572217 40.33926154478121 24.680521596451612 3 19.68363245514901 24.240444863779476 27.753873376948786 28.322049304122732 4 12.876418849423235 15.99431307163316 35.89729398144945 35.23197409749416 5 14.37724928921309 38.13481220019077 32.8722242446305 14.615714265965634 6 33.09176211418215 35.323511485386476 17.225948205440766 14.358778194990604 7 30.12693844113557 32.172217799034684 19.584722220621938 18.116121539207814 8 26.742675178328696 36.04643521310092 18.823347656506705 18.38754195206368 9 26.72168971016047 17.698002820334544 18.90788713588455 36.672420333620444 10-11 25.516200309400418 27.397435491165957 25.925622906001617 21.16074129343201 12-13 25.57854315195701 24.35791676075746 26.687124692956786 23.376415394328745 14-15 22.99174431449704 25.717894460385494 24.19870779573082 27.091653429386646 16-17 23.37042146829843 28.84613944485888 24.826219573664062 22.957219513178625 18-19 23.42077925220689 27.71050947028296 26.250772793297024 22.61793848421312 20-21 24.25167426234938 26.706028036897823 26.225301528792233 22.816996171960565 22-23 24.040990458620392 26.496209391484822 26.04531434666052 23.417485803234268 24-25 23.691093139808842 26.79279493196981 26.01463190848775 23.5014800197336 26-27 23.403869303030127 27.12860623304516 26.029673487959542 23.437850975965166 28-29 23.36490257113157 27.129598967976758 26.1415749296675 23.363923531224174 30-31 23.60272664549577 26.836874678203536 26.05569396882879 23.504704707471898 32-33 23.424562785685943 26.882021388039906 25.998694994387783 23.694720831886368 34-35 23.723966027332253 27.028105719108673 25.875506886048043 23.37242136751103 36-37 23.881066325737287 26.752935620785173 25.826840126005656 23.539157927471884 38-39 23.562578253773168 26.779636222901303 25.972074561519705 23.68571096180582 40-41 23.76885660742896 26.59033606428034 26.09966401634652 23.54114331194418 42-43 23.44590858827866 26.553457887946923 26.02636610762993 23.97426741614449 44-45 23.524868687799483 26.739368319203834 26.154683468485075 23.581079524511612 46-47 23.649650760079922 26.982621231782762 25.8464035516666 23.521324456470715 48-49 23.599342005225253 26.72637231998452 26.02333623625146 23.650949438538774 50-51 23.66410587055645 26.834106141245595 25.970953326855987 23.530834661341963 52-53 23.71698215357395 26.69315274573249 25.997864372691154 23.592000728002404 54-55 23.618745709138324 26.585027023789227 26.049677235049373 23.746550032023077 56-57 23.514294337327467 26.910884154700003 25.997919608895685 23.57690189907684 58-59 23.71695035526352 26.586417506059295 25.925827796260286 23.7708043424169 60-61 23.64299408929467 26.720327401126852 26.18195389039365 23.454724619184823 62-63 23.46983091303276 26.839474514845506 26.18233007785051 23.508364494271223 64-65 23.44352160042582 26.645536879773356 26.05755505092924 23.853386468871584 66-67 23.487012111737197 26.866789323975432 25.819152282082626 23.827046282204744 68-69 23.31925596367958 26.655425509668568 26.270722162726408 23.754596363925447 70-71 23.39164780677021 26.55607826285809 26.295659046097253 23.756614884274445 72-73 23.367422872322045 26.717704980728158 25.949135243852712 23.96573690309709 74-75 23.292133479004313 26.99228776968473 25.870037602234135 23.84554114907682 76-77 23.571854763095025 26.69566689780845 25.983178099738062 23.749300239358455 78-79 23.50112265317102 26.651002899659343 26.12360359571583 23.724270851453806 80-81 23.427776561798012 26.907737855242086 26.03044685336436 23.634038729595538 82-83 23.531563289298234 26.823971914302913 26.04994484812354 23.594519948275316 84-85 23.56185560181844 26.753284303250812 25.952460901974582 23.732399192956162 86-87 23.459569994671735 26.745409772324706 26.157975835601444 23.63704439740211 88-89 23.493724319364144 26.805673083957426 26.10537982139267 23.59522277528576 90-91 23.540241220364447 26.719723318928622 26.180151451089458 23.55988400961748 92-93 23.40625073809358 26.612039580826213 26.1423096265837 23.839400054496508 94-95 23.317241089311093 26.6868774959923 26.30070972153179 23.695171693164816 96-97 23.538479262672812 26.577937788018435 26.09130184331797 23.792281105990785 98-99 23.2342341511973 26.678721864803585 26.07828543513927 24.008758548859845 100-101 23.176406686408015 26.643622931377397 26.25505509702964 23.924915285184944 102-103 23.233102933408293 26.37532856327338 26.31311539908305 24.078453104235276 104-105 23.00986141031992 26.60904577030748 26.345460410354484 24.03563240901812 106-107 23.152613726963118 26.5943204494237 26.26746056251699 23.985605261096186 108-109 23.136029269883 26.613728667216936 26.174242664241334 24.07599939865873 110-111 23.01591644399696 26.754866717853336 26.323677560826415 23.90553927732329 112-113 23.065415844869218 26.70221516654071 26.470422204199 23.76194678439107 114-115 22.881906987807753 26.94994491054659 26.241355736185735 23.92679236545992 116-117 22.76414850924036 26.776597999419455 26.56108175103784 23.898171740302345 118-119 22.69860901252966 26.96540993720481 26.2501864961252 24.08579455414033 120-121 22.72257038846136 26.917135155061978 26.45857333763421 23.90172111884245 122-123 22.75349288348569 26.904288915908726 26.35647484438475 23.985743356220837 124-125 22.74025056201067 26.924328567882196 26.22946853921 24.105952330897136 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 517.0 1 471.5 2 399.0 3 325.5 4 232.0 5 186.5 6 155.0 7 129.0 8 149.5 9 172.5 10 170.0 11 148.5 12 139.5 13 153.0 14 175.5 15 200.5 16 231.0 17 262.5 18 308.0 19 378.0 20 473.0 21 660.0 22 884.5 23 1218.5 24 1641.0 25 2113.0 26 2880.5 27 3861.0 28 4709.5 29 5788.0 30 7248.5 31 8879.5 32 10815.0 33 12663.0 34 14101.0 35 15475.5 36 17782.5 37 19831.0 38 20898.0 39 21757.0 40 22539.0 41 23738.5 42 24907.0 43 26436.5 44 28501.5 45 30313.0 46 31146.5 47 31789.5 48 32433.0 49 32673.0 50 33082.0 51 33246.5 52 33705.0 53 34950.0 54 35506.0 55 33899.5 56 30788.5 57 28003.0 58 26420.0 59 24144.0 60 20463.0 61 16662.0 62 13248.0 63 10261.0 64 7853.5 65 6091.0 66 4423.5 67 3354.5 68 2611.0 69 1811.0 70 1331.0 71 939.0 72 679.0 73 552.5 74 414.0 75 295.5 76 204.5 77 126.0 78 83.5 79 55.5 80 39.0 81 26.0 82 21.0 83 20.5 84 17.5 85 12.0 86 11.0 87 8.0 88 8.5 89 8.5 90 6.5 91 9.0 92 6.5 93 2.0 94 3.0 95 3.5 96 3.0 97 5.0 98 6.5 99 5.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04987094597007052 2 0.18358957939097556 3 0.02303507897000948 4 0.11390846550669688 5 0.021768149626658958 6 0.035934723193214786 7 0.03915963424901611 8 0.1015846982577418 9 0.11148978221484587 10-11 0.04992853366749554 12-13 0.03282498753226351 14-15 0.012899644223205307 16-17 0.03034871654298749 18-19 0.05154098919539621 20-21 0.20040518703908247 22-23 0.1112594314251458 24-25 0.07889514547228246 26-27 0.014512099751105971 28-29 0.005010129675977061 30-31 0.007831926849803224 32-33 0.005240480465677157 34-35 0.008350216126628436 36-37 0.0029945602661012325 38-39 0.008292628429203412 40-41 0.0050677173734020855 42-43 0.00932920698285384 44-45 0.00932920698285384 46-47 0.016297318371281705 48-49 0.017161133832657063 50-51 0.0099626716545291 52-53 0.012899644223205307 54-55 0.013475521197455544 56-57 0.015548678304756397 58-59 0.017621835412057252 60-61 0.008119865336928342 62-63 0.019234290939957915 64-65 0.031327707399212895 66-67 0.027642094764011377 68-69 0.034495030757589196 70-71 0.02505064837988531 72-73 0.015548678304756397 74-75 0.008580566916328532 76-77 0.010020259351954122 78-79 0.02395648212880986 80-81 0.019694992519358103 82-83 0.021134684954983697 84-85 0.015491090607331375 86-87 0.02631757772323583 88-89 0.028505910225386732 90-91 0.02741174397431128 92-93 0.03403432917818901 94-95 0.025856876143835642 96-97 0.02775727015886142 98-99 0.0332856891116637 100-101 0.020904334165283603 102-103 0.029657664173887207 104-105 0.02430200831336 106-107 0.03282498753226351 108-109 0.020904334165283603 110-111 0.020328457191033364 112-113 0.025914463841260664 114-115 0.012381354946380095 116-117 0.010711311721054408 118-119 0.03000319035843735 120-121 0.023150254364859527 122-123 0.01779459850433232 124-125 0.01756424771463223 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 868241.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.30421877270469 #Duplication Level Percentage of deduplicated Percentage of total 1 79.74037273292419 49.68161627769124 2 11.625686163486831 14.486585882253788 3 3.6925517830064805 6.901846623539297 4 1.623759061353779 4.046681591709899 5 0.8312922368802215 2.589650669531818 6 0.5458804222887773 2.040639195240984 7 0.3529950689149446 1.5395157399553872 8 0.25812568915571954 1.2865855526410495 9 0.18699546958640698 1.0485545981954532 >10 1.0710770422362657 12.041503576663882 >50 0.05071962402056246 2.1725455085192507 >100 0.02035961870894182 2.0836680042234152 >500 1.850874368801623E-4 0.080606779834631 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.151753948500474E-4 5.75876974250237E-5 24-25 0.0 0.0 0.0 1.151753948500474E-4 1.151753948500474E-4 26-27 0.0 0.0 0.0 2.303507897000948E-4 1.151753948500474E-4 28-29 0.0 0.0 0.0 8.638154613753554E-4 1.151753948500474E-4 30-31 0.0 0.0 0.0 0.0031097356609512796 1.151753948500474E-4 32-33 0.0 0.0 0.0 0.007198462178127962 1.151753948500474E-4 34-35 0.0 0.0 0.0 0.01370587198715564 2.303507897000948E-4 36-37 0.0 0.0 0.0 0.02585687614383564 2.303507897000948E-4 38-39 0.0 0.0 0.0 0.04399700083271811 2.303507897000948E-4 40-41 0.0 0.0 0.0 0.06749278138212778 2.303507897000948E-4 42-43 0.0 0.0 0.0 0.08880022942938653 2.303507897000948E-4 44-45 0.0 0.0 0.0 0.1144843424809471 2.303507897000948E-4 46-47 0.0 0.0 0.0 0.14707897922351051 2.303507897000948E-4 48-49 0.0 0.0 0.0 0.18191953616564988 3.455261845501422E-4 50-51 0.0 0.0 0.0 0.22568618620866787 3.455261845501422E-4 52-53 0.0 0.0 0.0 0.2615633217044576 4.6070157940018955E-4 54-55 0.0 0.0 0.0 0.3049844455629255 5.758769742502369E-4 56-57 0.0 0.0 0.0 0.35237912054372 5.758769742502369E-4 58-59 0.0 0.0 0.0 0.4051870390824667 5.758769742502369E-4 60-61 0.0 0.0 0.0 0.4670362261169422 5.758769742502369E-4 62-63 0.0 0.0 0.0 0.5281367730848923 5.758769742502369E-4 64-65 0.0 0.0 0.0 0.5909649509755932 5.758769742502369E-4 66-67 0.0 0.0 0.0 0.6657137822332739 5.758769742502369E-4 68-69 0.0 0.0 0.0 0.7564144056776863 5.758769742502369E-4 70-71 0.0 0.0 0.0 0.8575384023560279 6.334646716752606E-4 72-73 0.0 0.0 0.0 0.9606779684442454 8.062277639503318E-4 74-75 0.0 0.0 0.0 1.0679062610496395 9.214031588003792E-4 76-77 0.0 0.0 0.0 1.183427182084237 9.214031588003792E-4 78-79 0.0 0.0 0.0 1.317030640110292 9.214031588003792E-4 80-81 0.0 0.0 0.0 1.47027150295828 9.214031588003792E-4 82-83 0.0 0.0 0.0 1.6301349510101457 0.0010365785536504265 84-85 0.0 0.0 0.0 1.793396073210088 0.0010365785536504265 86-87 0.0 0.0 0.0 1.9647770607469586 0.0010365785536504265 88-89 0.0 0.0 0.0 2.160056942715214 0.0010365785536504265 90-91 0.0 0.0 0.0 2.3885073384002826 0.0010365785536504265 92-93 0.0 0.0 0.0 2.6309515445596325 0.0010365785536504265 94-95 0.0 0.0 0.0 2.862914789787628 0.0010365785536504265 96-97 0.0 0.0 0.0 3.105358995946978 0.0010365785536504265 98-99 0.0 0.0 0.0 3.375157358383214 0.0010365785536504265 100-101 0.0 0.0 0.0 3.6871099153345672 0.0010365785536504265 102-103 0.0 0.0 0.0 4.014035273616427 0.0011517539485004738 104-105 0.0 0.0 0.0 4.3485046202609645 0.0012093416459254976 106-107 0.0 0.0 0.0 4.683492256182327 0.0012669293433505214 108-109 0.0 0.0 0.0 5.041918084955675 0.0012669293433505214 110-111 0.0 0.0 0.0 5.453727709242019 0.0013245170407755451 112-113 0.0 0.0 0.0 5.884195747494071 0.0013821047382005687 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1125 0.0 59.382404 1 GTATCAA 1895 0.0 52.56535 1 ATCAACG 2270 0.0 41.48321 3 CAACGCA 2285 0.0 40.668106 5 TCAACGC 2310 0.0 40.51156 4 AACGCAG 2440 0.0 38.084682 6 TATCAAC 2595 0.0 37.22799 2 GTACATG 5050 0.0 34.489246 1 TACATGG 5155 0.0 33.663265 2 ACATGGG 5270 0.0 31.552565 3 ACGCAGA 2970 0.0 31.087858 7 CGCAGAG 2980 0.0 30.583748 8 CATGGGG 2720 0.0 28.707388 4 TATACCG 85 0.007169116 28.032166 5 GTGGTAT 585 0.0 27.529615 1 GTGTAGG 525 0.0 27.267431 1 CATGGGA 2640 0.0 27.093716 4 GCAGAGT 3465 0.0 25.440397 9 GTATACC 170 1.3802983E-5 24.540886 3 GTATAGG 275 2.055458E-9 23.859001 1 >>END_MODULE