FastQCFastQC Report
Fri 27 May 2016
SRR940036_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940036_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868241
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34560.3980461646017638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25470.29335173068307074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15320.17644870491027262No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17100.064.852581
GTATCAA30550.056.597491
TCAACGC39850.043.2319344
ATCAACG40000.043.0698173
TATCAAC40500.042.6877442
CAACGCA41300.041.9398655
AACGCAG43600.040.1231356
GTGGTAT7700.035.6186521
ACGCAGA51350.034.063627
CGCAGAG52400.033.153978
GTATAGG2203.6379788E-1232.521381
GTACATG45850.031.5992891
TGGTATC8850.030.9866622
TACATGG47200.030.5656492
ACATGGG46700.029.4869793
GCAGAGT58200.029.2366099
TAACGCA1159.936292E-425.918354
TATTCCG1150.001002360625.8795225
CATGGGA25000.025.2755744
AGAGTAC54900.024.87106110-11
Produced by FastQC (version 0.11.5)