FastQCFastQC Report
Fri 27 May 2016
SRR940035_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940035_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744764
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19640.2637076980090337No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9500.053.3789251
GTATCAA17300.051.0375861
ATCAACG19350.044.0503653
TCAACGC19950.042.725544
CAACGCA20600.041.641545
TATCAAC22200.040.0304832
AACGCAG21950.039.0804446
GTACATG42450.034.713131
TACATGG42100.034.2839472
ACATGGG43700.031.9175013
CGCAGAG26350.031.6503818
ACGCAGA26900.031.4461577
ATACCGT953.262463E-431.3529196
TAGCCCT7500.027.0216664
CATGGGA30300.026.9508464
GCAGAGT30850.026.8279069
CCGTAAG1358.094233E-526.5150221
TGGTATC4900.025.561172
GTGGTAT5200.025.2402611
CATGGGG15050.024.1594894
Produced by FastQC (version 0.11.5)