##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940035_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 744764 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.986454769564588 33.0 31.0 34.0 27.0 34.0 2 31.03564216315504 34.0 31.0 34.0 27.0 34.0 3 31.13022514514665 34.0 31.0 34.0 27.0 34.0 4 34.24486145946904 37.0 35.0 37.0 30.0 37.0 5 34.42135361000263 37.0 35.0 37.0 31.0 37.0 6 34.37643199725014 37.0 35.0 37.0 31.0 37.0 7 34.33245296496608 37.0 35.0 37.0 31.0 37.0 8 34.28733397425225 37.0 35.0 37.0 30.0 37.0 9 35.822844552099724 39.0 37.0 39.0 30.0 39.0 10-11 35.895875069149426 39.0 37.5 39.0 30.5 39.0 12-13 35.881030098125045 39.0 38.0 39.0 30.5 39.0 14-15 37.307846512452265 41.0 39.0 41.0 31.0 41.0 16-17 37.37743835631153 41.0 39.0 41.0 31.0 41.0 18-19 37.39190468389987 41.0 39.0 41.0 31.0 41.0 20-21 37.398282543194895 41.0 39.0 41.0 31.0 41.0 22-23 37.46086679807294 41.0 39.0 41.0 30.5 41.0 24-25 37.56999129925721 41.0 39.0 41.0 30.5 41.0 26-27 37.625847919609434 41.0 39.0 41.0 30.0 41.0 28-29 37.59340005693079 41.0 39.0 41.0 30.0 41.0 30-31 37.555341020779736 41.0 38.5 41.0 30.0 41.0 32-33 37.50239136155883 41.0 38.0 41.0 30.0 41.0 34-35 37.42012033342105 41.0 38.0 41.0 30.0 41.0 36-37 37.297792992142476 41.0 38.0 41.0 30.0 41.0 38-39 37.20331406996041 40.5 38.0 41.0 30.0 41.0 40-41 37.10563950459475 40.0 38.0 41.0 30.0 41.0 42-43 36.96579990439925 40.0 38.0 41.0 30.0 41.0 44-45 36.82905994382113 40.0 38.0 41.0 30.0 41.0 46-47 36.68402406668421 40.0 37.0 41.0 29.5 41.0 48-49 36.55540077125103 40.0 37.0 41.0 29.0 41.0 50-51 36.41196741518118 40.0 37.0 41.0 29.0 41.0 52-53 36.56808196959037 40.0 37.0 41.0 30.0 41.0 54-55 36.40619914496404 40.0 37.0 41.0 29.0 41.0 56-57 36.31486551444485 40.0 36.0 41.0 29.5 41.0 58-59 36.147502430300065 40.0 36.0 41.0 29.0 41.0 60-61 35.932038202705826 39.5 35.0 41.0 29.0 41.0 62-63 35.6723915226837 39.0 35.0 41.0 28.5 41.0 64-65 35.39048074289305 39.0 35.0 41.0 28.0 41.0 66-67 35.08511421067613 38.5 35.0 41.0 28.0 41.0 68-69 34.74415183870327 37.5 35.0 40.0 28.0 41.0 70-71 34.38919912885156 37.0 35.0 40.0 27.5 41.0 72-73 34.00544602048434 36.5 34.0 39.0 26.5 41.0 74-75 33.62528331122342 36.0 34.0 39.0 26.0 41.0 76-77 33.24336971174762 35.5 34.0 38.5 26.0 40.0 78-79 32.871027600689615 35.0 34.0 37.0 26.0 39.0 80-81 32.51034421642292 35.0 34.0 37.0 25.5 39.0 82-83 32.16201038181222 35.0 33.5 36.0 24.5 38.5 84-85 31.876484362831718 35.0 33.0 36.0 24.0 37.0 86-87 31.62977131010629 35.0 33.0 36.0 23.5 37.0 88-89 31.42553882840739 35.0 33.0 35.0 23.0 36.5 90-91 31.24176316255888 35.0 33.0 35.0 21.0 36.0 92-93 31.068673432120782 35.0 33.0 35.0 20.0 36.0 94-95 30.937641319934905 35.0 33.0 35.0 20.0 36.0 96-97 30.812770219828025 35.0 33.0 35.0 19.0 35.5 98-99 30.73241859166125 35.0 33.0 35.0 18.5 35.0 100-101 30.626089606909034 35.0 32.0 35.0 18.0 35.0 102-103 29.920141816736578 34.0 31.0 35.0 17.5 35.0 104-105 30.802799947365877 35.0 33.0 35.0 19.0 35.0 106-107 30.903909829153932 35.0 33.0 35.0 19.0 35.0 108-109 30.88457417383225 35.0 33.0 35.0 18.0 35.0 110-111 30.83369026966932 35.0 33.0 35.0 18.0 35.0 112-113 30.774330526180105 35.0 33.0 35.0 16.5 35.0 114-115 30.707728219946183 35.0 33.0 35.0 12.5 35.0 116-117 30.62565994059863 35.0 33.0 35.0 6.0 35.0 118-119 30.537597816220977 35.0 33.0 35.0 2.0 35.0 120-121 30.435780999081587 35.0 33.0 35.0 2.0 35.0 122-123 30.294281812762165 35.0 33.0 35.0 2.0 35.0 124-125 29.07382741378477 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4472296429145146 1101 2 0.6162225675657673 1101 3 0.6290995135107487 1101 4 0.6351452939711066 1101 5 0.657908356074941 1101 6 0.6368443443201599 1101 7 0.7607888612441656 1101 8 0.7576301625013784 1101 9 0.8519936889284381 1101 10-11 0.7669317806586378 1101 12-13 0.8775506574466974 1101 14-15 1.0002426772540431 1101 16-17 0.89281707654402 1101 18-19 0.8816319459838695 1101 20-21 0.7623307406013495 1101 22-23 0.8916974546250813 1101 24-25 0.8135344211337738 1101 26-27 0.9117091526094114 1101 28-29 0.9051269592329092 1101 30-31 0.8257071596372825 1101 32-33 0.9356098309691561 1101 34-35 0.9080457615842334 1101 36-37 0.8543693806349779 1101 38-39 0.9752431647238353 1101 40-41 1.023297170120152 1101 42-43 0.9099551237949584 1101 44-45 1.011300109074213 1101 46-47 1.0126634595540196 1101 48-49 1.0327540179695234 1101 50-51 1.0205371701560324 1101 52-53 1.0296896023854885 1101 54-55 1.1749018534813942 1101 56-57 1.088165545945401 1101 58-59 0.9853225997314112 1101 60-61 0.9024454414312757 1101 62-63 0.9298961285301104 1101 64-65 0.9299130375714029 1101 66-67 0.9009698792038634 1101 68-69 0.9457474873436453 1101 70-71 0.9768672393677633 1101 72-73 1.0445793637670846 1101 74-75 0.9362671423907258 1101 76-77 0.8116724410511651 1101 78-79 0.6492059321528032 1101 80-81 0.5496847993022911 1101 82-83 0.8170014551428153 1101 84-85 0.8765020609896332 1101 86-87 0.780900333959547 1101 88-89 0.7591774459935294 1101 90-91 0.7832093377923037 1101 92-93 0.8706647864903871 1101 94-95 0.8333196244067587 1101 96-97 0.6741358926752348 1101 98-99 0.8698933596815657 1101 100-101 0.8438879165420943 1101 102-103 0.7890995883179066 1101 104-105 0.9418590911104552 1101 106-107 0.8775646453102333 1101 108-109 0.8208532321839641 1101 110-111 0.8850380585631186 1101 112-113 0.7806538347518064 1101 114-115 0.8448331850906392 1101 116-117 0.8131106826524324 1101 118-119 0.7387508965057918 1101 120-121 0.6564826139870377 1101 122-123 0.711058105147842 1101 124-125 0.7170898197474678 1102 1 0.4526762660081971 1102 2 0.5236299749731756 1102 3 0.48748731307501814 1102 4 0.5305701305724142 1102 5 0.5282787264453077 1102 6 0.5910927103332284 1102 7 0.6649282947953665 1102 8 0.6802881145710984 1102 9 0.6319501159436882 1102 10-11 0.7004829789157228 1102 12-13 0.7686181955730547 1102 14-15 0.8248614136374854 1102 16-17 0.7179804752368284 1102 18-19 0.7449217063324554 1102 20-21 0.5172327013856659 1102 22-23 0.5474708751043877 1102 24-25 0.6201793013080632 1102 26-27 0.5446067560952486 1102 28-29 0.6240812075989197 1102 30-31 0.7636156563693035 1102 32-33 0.7972656043896365 1102 34-35 0.7713355219328193 1102 36-37 0.7236504263866195 1102 38-39 0.8646767159220872 1102 40-41 0.7885477147824602 1102 42-43 0.7503690671500749 1102 44-45 0.6213218955665951 1102 46-47 0.7005719562860477 1102 48-49 0.7359130593638525 1102 50-51 0.5788160372584343 1102 52-53 0.6707571405118529 1102 54-55 0.7506099144835687 1102 56-57 0.8594073760107577 1102 58-59 0.7870655191213913 1102 60-61 0.716170931627353 1102 62-63 0.6804407908394765 1102 64-65 0.6967975691618165 1102 66-67 0.6378979837790268 1102 68-69 0.565573195404653 1102 70-71 0.6141221413285507 1102 72-73 0.5048190151832159 1102 74-75 0.6563107153754757 1102 76-77 0.5872715695914721 1102 78-79 0.6595545160308021 1102 80-81 0.574739265533232 1102 82-83 0.6312716076482587 1102 84-85 0.4821665490070721 1102 86-87 0.5913578502994135 1102 88-89 0.5609203653835095 1102 90-91 0.6198106449818503 1102 92-93 0.4245863551178388 1102 94-95 0.4139296461932531 1102 96-97 0.5908025593419026 1102 98-99 0.556712531794858 1102 100-101 0.7888770232959068 1102 102-103 0.5886638584704116 1102 104-105 0.5317283721562092 1102 106-107 0.5627498304954166 1102 108-109 0.5806571537525897 1102 110-111 0.5930990607417677 1102 112-113 0.5644228979326336 1102 114-115 0.4962493070949954 1102 116-117 0.43293639071343293 1102 118-119 0.4021495893162488 1102 120-121 0.5230403481918344 1102 122-123 0.45506681974478624 1102 124-125 0.4093556149544426 1103 1 0.25283049021861004 1103 2 0.4020436953535338 1103 3 0.32121549754947054 1103 4 0.43045910772215024 1103 5 0.2336663541331987 1103 6 0.3726819629496987 1103 7 0.2839132021911581 1103 8 0.29772148924767095 1103 9 0.24627810252803783 1103 10-11 0.32685508663111307 1103 12-13 0.4073061749107296 1103 14-15 0.478091528237222 1103 16-17 0.6653514634330975 1103 18-19 0.6147671569505135 1103 20-21 0.5224714469668186 1103 22-23 0.49466069630649656 1103 24-25 0.42706457614767857 1103 26-27 0.500190648026809 1103 28-29 0.47939149091622824 1103 30-31 0.6225248917052326 1103 32-33 0.5902974108103862 1103 34-35 0.5724490021507762 1103 36-37 0.5951925755845977 1103 38-39 0.6099652127470208 1103 40-41 0.49374953016864964 1103 42-43 0.5271353292104521 1103 44-45 0.5013034072425739 1103 46-47 0.30694020839618474 1103 48-49 0.3031875645224673 1103 50-51 0.20304781952357587 1103 52-53 0.28651820447309717 1103 54-55 0.2882241309827762 1103 56-57 0.2900645476688055 1103 58-59 0.3579250403759957 1103 60-61 0.26705446912195185 1103 62-63 0.2291131619438076 1103 64-65 0.22465896247535966 1103 66-67 0.2779946526289905 1103 68-69 0.3213949270823946 1103 70-71 0.23010269607117095 1103 72-73 0.16376750336786472 1103 74-75 0.10609494084012283 1103 76-77 0.0630102896234277 1103 78-79 0.16332650575538565 1103 80-81 0.05661908213648559 1103 82-83 0.1070126503339921 1103 84-85 0.23362982088297812 1103 86-87 0.2384317710783428 1103 88-89 0.2686678093030359 1103 90-91 0.2250222155138779 1103 92-93 0.17502417535372672 1103 94-95 0.16200997285127983 1103 96-97 0.21518995940972374 1103 98-99 0.109561034379972 1103 100-101 0.13366800768302767 1103 102-103 0.1484315466402073 1103 104-105 0.20089537560767567 1103 106-107 0.18531510867966006 1103 108-109 0.1667892848803305 1103 110-111 0.20369454271565246 1103 112-113 0.05906105170160458 1103 114-115 0.026979177816084388 1103 116-117 0.041093086227459 1103 118-119 0.1549298058804851 1103 120-121 0.19629057697322594 1103 122-123 0.1530324073065117 1103 124-125 0.22083858293433423 1104 1 0.20131546752067564 1104 2 0.2654097462264353 1104 3 0.30418701382508573 1104 4 0.2768112176566575 1104 5 0.3007276150598699 1104 6 0.38566643349160756 1104 7 0.27910848943355404 1104 8 0.4108934498613266 1104 9 0.5080460130876645 1104 10-11 0.3487671497602278 1104 12-13 0.4146374170660181 1104 14-15 0.4518574609779549 1104 16-17 0.5223975551153046 1104 18-19 0.48401017137918956 1104 20-21 0.34118275476600246 1104 22-23 0.37308692075314553 1104 24-25 0.4187125422831244 1104 26-27 0.3980600180109519 1104 28-29 0.3701376924523956 1104 30-31 0.37618072436922745 1104 32-33 0.5020260558478213 1104 34-35 0.4127602625181055 1104 36-37 0.44244521957496374 1104 38-39 0.4427365979138216 1104 40-41 0.4436775993117692 1104 42-43 0.5144685626087409 1104 44-45 0.5515313933799462 1104 46-47 0.5582356350255822 1104 48-49 0.5371261760667565 1104 50-51 0.5619497944339145 1104 52-53 0.45278419874099995 1104 54-55 0.5479538141513984 1104 56-57 0.5012476144962648 1104 58-59 0.4781105392047422 1104 60-61 0.5127609912258677 1104 62-63 0.6600008088539084 1104 64-65 0.5641737256405008 1104 66-67 0.4960760340594206 1104 68-69 0.5266764194854687 1104 70-71 0.543825268936132 1104 72-73 0.5165335412641312 1104 74-75 0.45334199145126064 1104 76-77 0.49288553489310516 1104 78-79 0.4467441499582918 1104 80-81 0.4366287922428356 1104 82-83 0.38290216543276046 1104 84-85 0.4149073263253449 1104 86-87 0.5496966131101253 1104 88-89 0.6747896510014542 1104 90-91 0.6008754855152425 1104 92-93 0.5411589489449256 1104 94-95 0.44380777442950503 1104 96-97 0.6681183171712064 1104 98-99 0.7580512694585124 1104 100-101 0.6196476433079319 1104 102-103 0.6641976046726334 1104 104-105 0.71673638492177 1104 106-107 0.5617696669481269 1104 108-109 0.5814555726887747 1104 110-111 0.6062601139003512 1104 112-113 0.6893688889208462 1104 114-115 0.7266460361492157 1104 116-117 0.8395882434797279 1104 118-119 0.741408482800189 1104 120-121 0.669173874471646 1104 122-123 0.9152824671556701 1104 124-125 0.9284262255934728 1105 1 0.21563108448802737 1105 2 0.24833626455027158 1105 3 0.24432358860288161 1105 4 0.1434628473683759 1105 5 0.030325027831906937 1105 6 0.3660606414309058 1105 7 0.2953723727380648 1105 8 0.11574308575998771 1105 9 0.27788907304262267 1105 10-11 0.4474542492514715 1105 12-13 0.5097407089605497 1105 14-15 0.6399103823423147 1105 16-17 0.5699290428258621 1105 18-19 0.38231404644606215 1105 20-21 0.3172652793181854 1105 22-23 0.29569945002344156 1105 24-25 0.18137491143165363 1105 26-27 0.18065016619033258 1105 28-29 0.2997118960113596 1105 30-31 0.26969347690562273 1105 32-33 0.29811568481640904 1105 34-35 0.3580437254634461 1105 36-37 0.4001973691512646 1105 38-39 0.37343203528763524 1105 40-41 0.37274130914647685 1105 42-43 0.41053541821801787 1105 44-45 0.38736652733415156 1105 46-47 0.30414433019927145 1105 48-49 0.18851725784490725 1105 50-51 0.12730116339611186 1105 52-53 0.2030143840115386 1105 54-55 0.38172082198260426 1105 56-57 0.33204700299343415 1105 58-59 0.40004477105061653 1105 60-61 0.3644208858146314 1105 62-63 0.26828922200841276 1105 64-65 0.32496628212987133 1105 66-67 0.22543590652860956 1105 68-69 0.2887422137908189 1105 70-71 0.27627371015961444 1105 72-73 0.23972117957960393 1105 74-75 0.25030823130811797 1105 76-77 0.30993155778196524 1105 78-79 0.2197422463669696 1105 80-81 0.25452364034234165 1105 82-83 0.2970831035862034 1105 84-85 0.4599321100601479 1105 86-87 0.24766575248417766 1105 88-89 0.1283668663099462 1105 90-91 0.27484597167742564 1105 92-93 0.2865271854478948 1105 94-95 0.20969655108605068 1105 96-97 0.35977411473456655 1105 98-99 0.40293450968258426 1105 100-101 0.30282445065729746 1105 102-103 0.22964486146350893 1105 104-105 0.4947625993465685 1105 106-107 0.4851787189770853 1105 108-109 0.5182551420652572 1105 110-111 0.5255100313605432 1105 112-113 0.3934040230929803 1105 114-115 0.4726048545060699 1105 116-117 0.3490991174863858 1105 118-119 0.33892803901388646 1105 120-121 0.4646494944463875 1105 122-123 0.40125723776038313 1105 124-125 0.464402140314327 1106 1 0.2979052914970559 1106 2 0.4079410272741235 1106 3 0.4334086945792208 1106 4 0.3795349380359596 1106 5 0.29715478402580686 1106 6 0.3256450179638506 1106 7 0.2854022512491099 1106 8 0.3719624666568535 1106 9 0.4864684740708469 1106 10-11 0.512457530154073 1106 12-13 0.5771744060599815 1106 14-15 0.5428466309009465 1106 16-17 0.5486573267545367 1106 18-19 0.521245937456392 1106 20-21 0.5849646339678358 1106 22-23 0.6189352481746724 1106 24-25 0.7700516565214528 1106 26-27 0.7526978316529451 1106 28-29 0.7256590575528108 1106 30-31 0.6439536811497746 1106 32-33 0.5718990433139481 1106 34-35 0.5748460877133255 1106 36-37 0.6306234405411502 1106 38-39 0.6461100799971007 1106 40-41 0.6146600956584649 1106 42-43 0.5472200502154152 1106 44-45 0.6389783105524884 1106 46-47 0.5828012624597818 1106 48-49 0.6012611754337343 1106 50-51 0.4868817175182585 1106 52-53 0.4455543122776291 1106 54-55 0.6758107378258558 1106 56-57 0.4707256592886253 1106 58-59 0.45362859970467895 1106 60-61 0.4448890037232687 1106 62-63 0.5617946275343471 1106 64-65 0.5341206374484386 1106 66-67 0.5323070084659278 1106 68-69 0.5715878712136231 1106 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-1.3723559113416428 2216 52-53 -1.607127755349829 2216 54-55 -1.3542935815436437 2216 56-57 -1.550682868358038 2216 58-59 -1.4170732863758886 2216 60-61 -1.3186755929316476 2216 62-63 -1.2852074515167544 2216 64-65 -1.190593411146601 2216 66-67 -1.2738712580482456 2216 68-69 -1.380269715750842 2216 70-71 -1.4986159783338664 2216 72-73 -1.492803102921627 2216 74-75 -1.398360679839307 2216 76-77 -1.3313479958231405 2216 78-79 -1.3914699794674483 2216 80-81 -1.4200845234741521 2216 82-83 -1.4566496595133103 2216 84-85 -1.2096159936063913 2216 86-87 -1.2371244644002566 2216 88-89 -1.2327520612353666 2216 90-91 -1.2733451724024043 2216 92-93 -1.3374391327055903 2216 94-95 -1.1721472359796472 2216 96-97 -1.1374399085470976 2216 98-99 -1.2714351697371775 2216 100-101 -1.3464155591082552 2216 102-103 -1.2337120276089841 2216 104-105 -1.2562001525172377 2216 106-107 -1.2445279884547809 2216 108-109 -1.1888906753441724 2216 110-111 -1.203444967930988 2216 112-113 -1.2182296249599318 2216 114-115 -1.1619592179692404 2216 116-117 -1.2281468766742343 2216 118-119 -1.3208535132700767 2216 120-121 -1.382705856617946 2216 122-123 -1.3379416143214122 2216 124-125 -1.28489587737144 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17699.0 3 3014.0 4 2270.0 5 1564.0 6 1571.0 7 1707.0 8 1909.0 9 1937.0 10 2277.0 11 2705.0 12 2900.0 13 2859.0 14 2946.0 15 2930.0 16 2888.0 17 2736.0 18 2339.0 19 2051.0 20 1841.0 21 1711.0 22 1709.0 23 2427.0 24 4419.0 25 7669.0 26 8251.0 27 5911.0 28 5807.0 29 6950.0 30 7870.0 31 9413.0 32 12455.0 33 16727.0 34 25088.0 35 43738.0 36 102002.0 37 262448.0 38 159128.0 39 898.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58043299534119 19.51488186395413 13.739461254493577 23.165223886211102 2 14.557322180235177 20.591887278015367 41.8226382960405 23.02815224570896 3 19.05068884286754 26.38379654625011 28.20280372074533 26.362710890137013 4 12.037324537173319 17.509654403927087 37.8812867878827 32.571734271016894 5 13.133780732985137 39.9680902069436 33.45532293220915 13.442806127862111 6 30.797273246247354 37.77359884482354 17.95829275664059 13.470835152288526 7 27.82649226165051 34.3371293020307 20.335431458989834 17.50094697732896 8 24.918888115328063 38.2043024915528 19.423977936492147 17.45283145662698 9 25.576402965894367 19.32576445981041 19.54070577101962 35.5571268032756 10-11 23.97731363814662 29.52585396259061 26.722567610409286 19.774264788853486 12-13 24.422207745982284 26.570720536187984 27.21201050348213 21.795061214347594 14-15 21.805823391329106 27.782172075009537 25.156516063879504 25.255488469781856 16-17 21.750757184014184 30.74180528786424 25.90214026204275 21.60529726607883 18-19 21.604767444672223 29.75206278496793 27.509397056887458 21.133772713472386 20-21 23.12779656730192 28.503326956554492 27.234697748754915 21.13417872738867 22-23 22.456357300554124 28.62168038254359 27.049165454136105 21.872796862766183 24-25 22.032652512765385 28.652244020424618 27.390083310937918 21.925020155872076 26-27 22.008934536691292 28.86998187711218 27.18359311263116 21.93749047356536 28-29 21.845573755742993 28.913075734697262 27.269956217944802 21.97139429161494 30-31 22.199692219270943 28.64878619982113 27.243563058792564 21.90795852211536 32-33 21.92508055186842 28.64853469118371 27.35554208268627 22.070842674261606 34-35 22.272586578611477 28.63930966738263 27.25805357839461 21.830050175611284 36-37 22.146795322831007 28.66075217127419 27.13602623253715 22.056426273357648 38-39 21.978128963512685 28.710250730679544 27.376798331103082 21.93482197470469 40-41 22.335472868633428 28.483167944569026 27.26786265794739 21.913496528850157 42-43 22.06462754492948 28.535976483448305 27.269370067426113 22.130025904196103 44-45 22.121252611061163 28.61984896635214 27.32101599570816 21.937882426878538 46-47 22.083897376393992 28.62881131953845 27.070776660435335 22.216514643632223 48-49 21.906455351615012 28.553451686594407 27.460526156751147 22.079566805039434 50-51 22.107054850167525 28.586142762181655 27.313825680003763 21.992976707647063 52-53 22.10850597804848 28.57967799248228 27.35304663589569 21.958769393573547 54-55 22.11252767263798 28.49269482645051 27.38270058088199 22.012076920029518 56-57 22.04371327849589 28.762531475574953 27.14070841027363 22.053046835655532 58-59 22.04138147764899 28.566421146145927 27.366461704372163 22.025735671832916 60-61 22.127073863445617 28.60350349476624 27.390708823193698 21.878713818594438 62-63 21.991466639448728 28.65182572820293 27.412581402001322 21.944126230347017 64-65 21.849746851509725 28.59990315953761 27.344872257222235 22.20547773173043 66-67 21.895849044440133 28.732136345657555 27.16947580696609 22.202538802936218 68-69 22.023380588349465 28.54461310948931 27.285591193629603 22.146415108531624 70-71 21.928573970819027 28.3996397874988 27.39958673606779 22.272199505614385 72-73 21.918074968575084 28.401948882132384 27.417637168426822 22.262338980865717 74-75 21.865505537632064 28.615474483920895 27.246156893420302 22.27286308502674 76-77 21.92752931318222 28.607772399581833 27.221594430310738 22.243103856925217 78-79 21.92463561794697 28.484150513878582 27.338877827507364 22.252336040667085 80-81 21.789892413333707 28.652285964848666 27.29083317553239 22.266988446285232 82-83 21.98900741009344 28.631231176784617 27.3757122184282 22.004049194693742 84-85 22.13922831784248 28.445422107266268 27.193933118730975 22.221416456160284 86-87 22.02059490727994 28.437062237342403 27.396706480264 22.145636375113657 88-89 22.029456956204065 28.3652141444965 27.466274659388834 22.139054239910603 90-91 21.982785540498707 28.51365626282237 27.357922284444868 22.145635912234056 92-93 22.0033955629341 28.441130367857127 27.346235935210295 22.209238133998475 94-95 21.912843417524392 28.62959359027824 27.33707918740888 22.12048380478849 96-97 21.942761284187664 28.497001874290255 27.360809940521 22.199426901001075 98-99 21.765313468760578 28.445429733461562 27.231406512757417 22.557850285020443 100-101 21.74586589560287 28.48412366925016 27.39111924673549 22.378891188411483 102-103 21.743697055738295 28.452264708213065 27.405971259836704 22.398066976211933 104-105 21.55883767093667 28.54283120280859 27.437031107720657 22.46130001853409 106-107 21.490496104487445 28.662926849251956 27.435274586456472 22.411302459804126 108-109 21.575937620031212 28.71609915686048 27.24134365120508 22.46661957190323 110-111 21.389380382413673 28.869714113045376 27.305679128405714 22.43522637613524 112-113 21.458498281184628 28.956750589447232 27.23551766259913 22.34923346676901 114-115 21.38712837581506 28.894176956127914 27.277317571192505 22.441377096864514 116-117 21.34276206769674 28.822075163091498 27.389434839943384 22.44572792926838 118-119 21.299091322400905 28.848885256536388 27.239577002552572 22.612446418510135 120-121 21.154229697632676 28.861651856194374 27.401489038370237 22.582629407802713 122-123 21.322654010487277 28.805633503958077 27.35105923653317 22.52065324902148 124-125 21.31253437153557 28.85852592025065 27.168873271686717 22.660066436527064 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1522.0 1 1323.0 2 991.5 3 703.0 4 465.0 5 332.5 6 273.0 7 276.0 8 329.0 9 323.5 10 249.5 11 223.5 12 226.5 13 248.5 14 263.5 15 280.0 16 325.0 17 386.5 18 492.0 19 607.5 20 758.0 21 970.0 22 1280.5 23 1795.5 24 2398.0 25 3148.5 26 4177.5 27 5551.5 28 6963.5 29 8382.5 30 10066.5 31 11657.0 32 12980.5 33 14654.0 34 16605.0 35 18185.5 36 19984.5 37 21731.5 38 22508.5 39 23118.0 40 24053.5 41 25161.5 42 26282.0 43 27045.0 44 27908.0 45 28881.0 46 29159.0 47 28923.5 48 29002.0 49 28805.5 50 27902.5 51 26816.5 52 25504.5 53 24279.5 54 22904.0 55 20883.0 56 18585.5 57 16482.0 58 14617.5 59 12532.0 60 10386.5 61 8377.5 62 6406.5 63 4727.5 64 3473.0 65 2469.5 66 1745.5 67 1393.0 68 1087.0 69 733.5 70 509.5 71 385.0 72 314.5 73 260.5 74 199.0 75 152.0 76 101.0 77 63.5 78 51.0 79 37.0 80 29.0 81 21.5 82 14.0 83 12.5 84 12.5 85 9.5 86 8.0 87 6.5 88 7.5 89 7.5 90 4.5 91 4.5 92 4.5 93 3.0 94 1.5 95 2.0 96 2.5 97 1.0 98 1.0 99 3.0 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.049411625696193694 2 0.1897245301867437 3 0.024437271404095795 4 0.10781938976642265 5 0.022154669130086846 6 0.038804238658152114 7 0.03893850938015264 8 0.09801762706038422 9 0.11278740648044212 10-11 0.04726329414418527 12-13 0.03343340977813106 14-15 0.01503832086405895 16-17 0.03074799533812053 18-19 0.05129141580420106 20-21 0.193618381124759 22-23 0.10694663007341923 24-25 0.07572868720829685 26-27 0.016850975611066056 28-29 0.007049212905027633 30-31 0.010473116316041054 32-33 0.007720566515030265 34-35 0.010674522399041843 36-37 0.005370828880021054 38-39 0.010808793121042371 40-41 0.0072506189880284224 42-43 0.013024260034051055 44-45 0.012822853951050265 46-47 0.01879790108007369 48-49 0.021080503354082635 50-51 0.013292801478052108 52-53 0.01651529880606474 54-55 0.016179622001063425 56-57 0.018663630358073162 58-59 0.020879097271081844 60-61 0.010607387038041581 62-63 0.021080503354082635 64-65 0.03229210864112658 66-67 0.026921279761105532 68-69 0.03457471091513553 70-71 0.02732409192710711 72-73 0.01772373530406948 74-75 0.01161441745304553 76-77 0.012151500341047634 78-79 0.02437013604309553 80-81 0.020811961910081583 82-83 0.02302742882309027 84-85 0.01758946458206895 86-87 0.028331122342111055 88-89 0.029539558840115793 90-91 0.028935340591113426 92-93 0.03531319988613843 94-95 0.027861174815109215 96-97 0.028935340591113426 98-99 0.03437330483213474 100-101 0.022154669130086846 102-103 0.031016536782121587 104-105 0.025511437180100006 106-107 0.03403762802713343 108-109 0.022288939852087374 110-111 0.02034201438307974 112-113 0.028263986981110795 114-115 0.014836914781058162 116-117 0.01208436498004737 118-119 0.029606694201116057 120-121 0.025444301819099742 122-123 0.019804931495077633 124-125 0.01926784860707553 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 744764.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.73545704347575 #Duplication Level Percentage of deduplicated Percentage of total 1 81.40550220759017 58.39660906715156 2 11.702090698407849 16.789096492289868 3 3.473620016802707 7.4754515850212435 4 1.398494635363518 4.012866073626037 5 0.693274224608454 2.486617167937436 6 0.35886111247272495 1.544583955101665 7 0.245259096651097 1.2315641374635506 8 0.16461972232552793 0.9447256815513452 9 0.11227335985858494 0.7248582704935992 >10 0.42857825464955085 5.085216608527833 >50 0.013117113371093728 0.6054350582570561 >100 0.004122185819673049 0.4389903077405595 >500 0.0 0.0 >1k 1.8737207912349562E-4 0.2639855948382855 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1964 0.2637076980090337 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 1.3427072200052634E-4 0.0 0.0 0.0 0.0 9 1.3427072200052634E-4 0.0 0.0 0.0 0.0 10-11 1.3427072200052634E-4 0.0 0.0 0.0 0.0 12-13 2.685414440010527E-4 0.0 0.0 0.0 0.0 14-15 4.02812166001579E-4 0.0 0.0 0.0 0.0 16-17 8.05624332003158E-4 0.0 0.0 0.0 0.0 18-19 0.0010741657760042107 0.0 0.0 0.0 0.0 20-21 0.0010741657760042107 0.0 0.0 0.0 0.0 22-23 0.0010741657760042107 0.0 0.0 6.713536100026317E-5 0.0 24-25 0.0013427072200052634 0.0 0.0 1.3427072200052634E-4 0.0 26-27 0.0014769779420057898 0.0 0.0 4.699475270018422E-4 0.0 28-29 0.0014769779420057898 0.0 0.0 9.398950540036844E-4 0.0 30-31 0.001611248664006316 0.0 0.0 0.002484008357009737 0.0 32-33 0.0017455193860068424 0.0 0.0 0.007921972598031055 0.0 34-35 0.0018126547470071056 0.0 0.0 0.014971185503058686 0.0 36-37 0.0018797901080073688 0.0 0.0 0.025377166458099478 0.0 38-39 0.0018797901080073688 0.0 0.0 0.04289949567916816 0.0 40-41 0.002014060830007895 0.0 0.0 0.06935082791327185 0.0 42-43 0.002014060830007895 6.713536100026317E-5 0.0 0.09358669323436686 0.0 44-45 0.002014060830007895 1.3427072200052634E-4 0.0 0.12138073268847582 0.0 46-47 0.002014060830007895 1.3427072200052634E-4 0.0 0.1568282032966148 0.0 48-49 0.0021483315520084215 1.3427072200052634E-4 0.0 0.19590098339876794 0.0 50-51 0.0021483315520084215 1.3427072200052634E-4 0.0 0.23712209505292953 0.0 52-53 0.0021483315520084215 1.3427072200052634E-4 0.0 0.28277414053310845 0.0 54-55 0.0021483315520084215 1.3427072200052634E-4 0.0 0.3278891031252853 0.0 56-57 0.0021483315520084215 1.3427072200052634E-4 0.0 0.3809260383154932 0.0 58-59 0.0021483315520084215 1.3427072200052634E-4 0.0 0.446181609207749 0.0 60-61 0.0021483315520084215 1.3427072200052634E-4 0.0 0.520903266001042 0.0 62-63 0.0021483315520084215 1.3427072200052634E-4 0.0 0.5931409144373251 0.0 64-65 0.0021483315520084215 1.3427072200052634E-4 0.0 0.6651100214296073 0.0 66-67 0.0022154669130086844 1.3427072200052634E-4 0.0 0.7512446895929449 0.0 68-69 0.002282602274008948 1.3427072200052634E-4 0.0 0.8520148664543399 0.0 70-71 0.002282602274008948 1.3427072200052634E-4 0.0 0.9680247702627947 0.0 72-73 0.002282602274008948 1.3427072200052634E-4 0.0 1.0822220193242424 0.0 74-75 0.002282602274008948 1.3427072200052634E-4 0.0 1.191854063837672 0.0 76-77 0.002282602274008948 1.3427072200052634E-4 0.0 1.3125634429161452 0.0 78-79 0.002282602274008948 1.3427072200052634E-4 0.0 1.4631480576397355 0.0 80-81 0.002282602274008948 1.3427072200052634E-4 0.0 1.640049733875429 0.0 82-83 0.002282602274008948 1.3427072200052634E-4 0.0 1.809230843596092 0.0 84-85 0.002282602274008948 1.3427072200052634E-4 0.0 1.9815001799227674 0.0 86-87 0.002282602274008948 1.3427072200052634E-4 0.0 2.1682707542254995 0.0 88-89 0.002282602274008948 1.3427072200052634E-4 0.0 2.394852597601388 0.0 90-91 0.002282602274008948 1.3427072200052634E-4 0.0 2.6443275990783657 0.0 92-93 0.002282602274008948 1.3427072200052634E-4 0.0 2.9024630621243777 0.0 94-95 0.002282602274008948 1.3427072200052634E-4 0.0 3.1649623236354065 0.0 96-97 0.002282602274008948 1.3427072200052634E-4 0.0 3.427797261951437 0.0 98-99 0.002282602274008948 1.3427072200052634E-4 0.0 3.7209773834395863 0.0 100-101 0.002349737635009211 1.3427072200052634E-4 0.0 4.056587053079902 0.0 102-103 0.002416872996009474 1.3427072200052634E-4 0.0 4.421199198672332 0.0 104-105 0.002819685162011053 1.3427072200052634E-4 0.0 4.779702026413736 0.0 106-107 0.0029539558840115795 1.3427072200052634E-4 0.0 5.137197823740138 0.0 108-109 0.0029539558840115795 1.3427072200052634E-4 0.0 5.514297146478616 0.0 110-111 0.0029539558840115795 1.3427072200052634E-4 0.0 5.9456418409053065 0.0 112-113 0.0029539558840115795 1.3427072200052634E-4 0.0 6.406056146645112 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 950 0.0 53.378925 1 GTATCAA 1730 0.0 51.037586 1 ATCAACG 1935 0.0 44.050365 3 TCAACGC 1995 0.0 42.72554 4 CAACGCA 2060 0.0 41.64154 5 TATCAAC 2220 0.0 40.030483 2 AACGCAG 2195 0.0 39.080444 6 GTACATG 4245 0.0 34.71313 1 TACATGG 4210 0.0 34.283947 2 ACATGGG 4370 0.0 31.917501 3 CGCAGAG 2635 0.0 31.650381 8 ACGCAGA 2690 0.0 31.446157 7 ATACCGT 95 3.262463E-4 31.352919 6 TAGCCCT 750 0.0 27.021666 4 CATGGGA 3030 0.0 26.950846 4 GCAGAGT 3085 0.0 26.827906 9 CCGTAAG 135 8.094233E-5 26.515022 1 TGGTATC 490 0.0 25.56117 2 GTGGTAT 520 0.0 25.240261 1 CATGGGG 1505 0.0 24.159489 4 >>END_MODULE