FastQCFastQC Report
Fri 27 May 2016
SRR940035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940035_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744764
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47300.6351005150624897No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34680.46565086389782534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22810.3062715168832006No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10330.13870165582654373No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19150.069.6913761
GTATCAA39200.059.4279141
TAACGCA1207.2759576E-1249.6465844
ATCAACG48550.047.857063
TCAACGC49500.046.9385874
TATCAAC49750.046.8224682
CAACGCA50300.046.2762685
AACGCAG52750.044.2190066
CTATACG600.001300041139.7172664
TGGTATC8300.038.7602272
ATAACGC1551.200533E-1038.4360663
ACGCAGA60450.038.3844537
GTGGTAT9150.037.7664951
CGCAGAG61150.037.750478
GCAGAGT66900.034.1501169
CCTAGCG750.00388528431.7738113
GTACATG40600.030.963941
TACATGG40450.030.4875412
TATAACG1602.5719964E-729.787952
ACATGGG40450.029.4565623
Produced by FastQC (version 0.11.5)