##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940035_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 744764 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.07548297178704 34.0 33.0 34.0 31.0 34.0 2 33.11576016026553 34.0 33.0 34.0 31.0 34.0 3 33.18002749864387 34.0 33.0 34.0 31.0 34.0 4 36.43224162284965 37.0 37.0 37.0 35.0 37.0 5 36.44258986739423 37.0 37.0 37.0 35.0 37.0 6 36.52501060738704 37.0 37.0 37.0 35.0 37.0 7 36.50665579968957 37.0 37.0 37.0 35.0 37.0 8 36.50662491742351 37.0 37.0 37.0 35.0 37.0 9 38.363998528392884 39.0 39.0 39.0 37.0 39.0 10-11 38.34871449210756 39.0 39.0 39.0 37.0 39.0 12-13 38.335967501114446 39.0 39.0 39.0 37.0 39.0 14-15 39.90953510105214 41.0 40.0 41.0 38.0 41.0 16-17 39.924854719078795 41.0 40.0 41.0 38.0 41.0 18-19 39.97759155920534 41.0 40.0 41.0 38.0 41.0 20-21 39.98397948880451 41.0 40.0 41.0 38.0 41.0 22-23 39.94315984660913 41.0 40.0 41.0 38.0 41.0 24-25 39.918250613617204 41.0 40.0 41.0 38.0 41.0 26-27 39.82872963784501 41.0 40.0 41.0 37.0 41.0 28-29 39.73827346649408 41.0 40.0 41.0 37.0 41.0 30-31 39.575207716806936 41.0 40.0 41.0 36.5 41.0 32-33 39.383715512565054 41.0 40.0 41.0 35.0 41.0 34-35 39.268307141591166 41.0 39.0 41.0 35.0 41.0 36-37 39.16736107008394 41.0 39.0 41.0 35.0 41.0 38-39 39.04655837285368 41.0 39.0 41.0 35.0 41.0 40-41 38.917229484776385 41.0 39.0 41.0 35.0 41.0 42-43 38.74503399734681 41.0 39.0 41.0 35.0 41.0 44-45 38.51164328565828 41.0 39.0 41.0 35.0 41.0 46-47 38.176741894076514 41.0 39.0 41.0 34.5 41.0 48-49 37.86709682530305 41.0 38.0 41.0 33.5 41.0 50-51 37.884964230279664 41.0 39.0 41.0 34.0 41.0 52-53 37.797266650912235 41.0 38.5 41.0 34.0 41.0 54-55 37.66413924410954 41.0 38.0 41.0 34.0 41.0 56-57 37.508123378681034 41.0 37.5 41.0 33.5 41.0 58-59 37.32228934266425 40.5 37.0 41.0 33.0 41.0 60-61 37.116484550810725 40.0 37.0 41.0 33.0 41.0 62-63 36.88114489959236 40.0 36.0 41.0 33.0 41.0 64-65 36.60778917885398 39.0 36.0 41.0 32.0 41.0 66-67 36.29825286936533 39.0 35.0 41.0 32.0 41.0 68-69 35.9568568029604 38.5 35.0 41.0 31.5 41.0 70-71 35.59496699625653 37.0 35.0 40.5 31.0 41.0 72-73 35.21724398601436 37.0 35.0 39.0 31.0 41.0 74-75 34.847876642802284 36.5 35.0 39.0 31.0 41.0 76-77 34.46924999060105 36.0 35.0 39.0 30.5 40.5 78-79 34.10934269110752 35.5 35.0 37.0 30.0 39.0 80-81 33.771487343641745 35.0 35.0 37.0 30.0 39.0 82-83 33.46417791407748 35.0 35.0 36.5 30.0 39.0 84-85 33.191504288606865 35.0 35.0 36.0 29.5 37.0 86-87 32.96742726554989 35.0 35.0 36.0 29.0 37.0 88-89 32.83595407404225 35.0 35.0 36.0 29.5 37.0 90-91 32.80859909984908 35.0 35.0 35.0 30.0 36.0 92-93 32.706403639273645 35.0 35.0 35.0 30.0 36.0 94-95 32.6192545558056 35.0 35.0 35.0 30.0 36.0 96-97 32.540970696757626 35.0 35.0 35.0 30.0 36.0 98-99 32.47902892728435 35.0 35.0 35.0 29.5 36.0 100-101 32.41987058987813 35.0 35.0 35.0 29.0 35.5 102-103 31.94623531749655 35.0 33.5 35.0 28.0 35.0 104-105 32.31875869402925 35.0 34.5 35.0 29.0 35.0 106-107 32.36370917498698 35.0 35.0 35.0 29.0 35.0 108-109 32.35160131263058 35.0 35.0 35.0 29.0 35.0 110-111 32.31064471429876 35.0 35.0 35.0 29.0 35.0 112-113 32.27315028653372 35.0 35.0 35.0 29.0 35.0 114-115 32.21414367504337 35.0 35.0 35.0 29.0 35.0 116-117 32.15849316024942 35.0 35.0 35.0 29.0 35.0 118-119 32.10324344356064 35.0 35.0 35.0 29.0 35.0 120-121 32.02358733773383 35.0 34.5 35.0 29.0 35.0 122-123 31.92027474475136 35.0 34.0 35.0 29.0 35.0 124-125 30.899987781364295 34.5 32.5 35.0 22.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17325769200020602 1101 2 0.21429996891288283 1101 3 0.23789838867212154 1101 4 0.053949900713739396 1101 5 0.12060912741441854 1101 6 0.12997297900939486 1101 7 0.15515885452970224 1101 8 0.1627671623869844 1101 9 0.2383470365641145 1101 10-11 0.22203645767712743 1101 12-13 0.22182483555921806 1101 14-15 0.3261056637228137 1101 16-17 0.3069094503910037 1101 18-19 0.1999732589792913 1101 20-21 0.22496537520851945 1101 22-23 0.23708663726260681 1101 24-25 0.32227793544103633 1101 26-27 0.2665726458354243 1101 28-29 0.42866673234153296 1101 30-31 0.4280312529832315 1101 32-33 0.42895367788015193 1101 34-35 0.4422612207394039 1101 36-37 0.4283213169435456 1101 38-39 0.47606823033736845 1101 40-41 0.5412989250982037 1101 42-43 0.5501064065828345 1101 44-45 0.5640717701019398 1101 46-47 0.6358744462020738 1101 48-49 0.5721893451976712 1101 50-51 0.42991239800016245 1101 52-53 0.42493546938891313 1101 54-55 0.40314878064245363 1101 56-57 0.42863056782825737 1101 58-59 0.5190904029857464 1101 60-61 0.5283134495585955 1101 62-63 0.5990486861505673 1101 64-65 0.5109350496305609 1101 66-67 0.49571181971908373 1101 68-69 0.4736524013185175 1101 70-71 0.43670394131069656 1101 72-73 0.4600149283003532 1101 74-75 0.3632455320773005 1101 76-77 0.33372731829186364 1101 78-79 0.36095665371220775 1101 80-81 0.43922196942572356 1101 82-83 0.360225839650937 1101 84-85 0.37189988588936984 1101 86-87 0.44815326235016784 1101 88-89 0.41568641386346883 1101 90-91 0.4276809240559487 1101 92-93 0.5185571013644719 1101 94-95 0.5618770160899302 1101 96-97 0.5297847768077943 1101 98-99 0.5437442563016077 1101 100-101 0.56065598843945 1101 102-103 0.43432619538449657 1101 104-105 0.4937820982706853 1101 106-107 0.4164350864689865 1101 108-109 0.5426422575012566 1101 110-111 0.6066256526870575 1101 112-113 0.5489894310545793 1101 114-115 0.5750195545615284 1101 116-117 0.4834416266323558 1101 118-119 0.48689905762107344 1101 120-121 0.49864209337092547 1101 122-123 0.6148020616616385 1101 124-125 0.6153650069864227 1102 1 0.10898753949475548 1102 2 0.1271539994139701 1102 3 0.1387698483671116 1102 4 0.04196732990764218 1102 5 0.06941087033381166 1102 6 0.06897080036016234 1102 7 0.12138979134887506 1102 8 0.10721160683142728 1102 9 0.19804202567086548 1102 10-11 0.13652447510632726 1102 12-13 0.14775076148514188 1102 14-15 0.2683714589297779 1102 16-17 0.25353254407291104 1102 18-19 0.25335016529736976 1102 20-21 0.22224206366168175 1102 22-23 0.14340472005127225 1102 24-25 0.1861123580989812 1102 26-27 0.11624584844980035 1102 28-29 0.17322010924785758 1102 30-31 0.09524258195926194 1102 32-33 0.24540247962306694 1102 34-35 0.22494095930149172 1102 36-37 0.15925813611566042 1102 38-39 0.2358721519060012 1102 40-41 0.31689805363850354 1102 42-43 0.27341795342379527 1102 44-45 0.29609791389278683 1102 46-47 0.39676769238943876 1102 48-49 0.3401632014068241 1102 50-51 0.34657906466683386 1102 52-53 0.343780785293049 1102 54-55 0.18310520765770377 1102 56-57 0.265776537327163 1102 58-59 0.09588778860666025 1102 60-61 0.08550299204225809 1102 62-63 0.1170225423597131 1102 64-65 0.11169757686367632 1102 66-67 0.16891443409816986 1102 68-69 0.1207112248479234 1102 70-71 0.10391527028673408 1102 72-73 0.13103889344196773 1102 74-75 0.16662243839537894 1102 76-77 0.15889071698467916 1102 78-79 0.2667300741915142 1102 80-81 0.27201064044969314 1102 82-83 0.26872257167708113 1102 84-85 0.2929238510309773 1102 86-87 0.39641034296018773 1102 88-89 0.31601321124909276 1102 90-91 0.32256109834789015 1102 92-93 0.3360952277261262 1102 94-95 0.28900119909646804 1102 96-97 0.2705255175485419 1102 98-99 0.29810155477655087 1102 100-101 0.2943161191584025 1102 102-103 0.27909743721455627 1102 104-105 0.2895337322576168 1102 106-107 0.27863552219883303 1102 108-109 0.2724897520546321 1102 110-111 0.29290016249098016 1102 112-113 0.34855370120708074 1102 114-115 0.32883219072710546 1102 116-117 0.42189478567374294 1102 118-119 0.34692084411344126 1102 120-121 0.4223893700593848 1102 122-123 0.33375631002765616 1102 124-125 0.24553929892542214 1103 1 0.2940844638366329 1103 2 0.22262668268296437 1103 3 0.21671810893170118 1103 4 0.18482447276478808 1103 5 0.2275626882749151 1103 6 0.12294230604369005 1103 7 0.140572588845977 1103 8 0.17253856680281388 1103 9 0.2642748206130534 1103 10-11 0.2604577963618553 1103 12-13 0.2716260163963966 1103 14-15 0.3494889805652974 1103 16-17 0.36297134446868995 1103 18-19 0.34181191815772394 1103 20-21 0.2921760073884201 1103 22-23 0.37537976545915797 1103 24-25 0.37719243855570994 1103 26-27 0.4101416820734727 1103 28-29 0.4908151406269141 1103 30-31 0.46702280316880973 1103 32-33 0.4405483655064444 1103 34-35 0.40373071732742716 1103 36-37 0.38492949918825303 1103 38-39 0.31806273958385844 1103 40-41 0.32079082135919634 1103 42-43 0.4044876283916281 1103 44-45 0.363338740571713 1103 46-47 0.36081649599047694 1103 48-49 0.277565359616716 1103 50-51 0.2971100233189503 1103 52-53 0.32196525974618595 1103 54-55 0.2825252410110153 1103 56-57 0.21007117060336356 1103 58-59 0.18543189812443472 1103 60-61 0.08777570875746221 1103 62-63 0.11345262351323981 1103 64-65 0.16478299013346032 1103 66-67 0.19299454707204688 1103 68-69 0.15706888565685517 1103 70-71 0.2153701341559895 1103 72-73 0.26399456327208526 1103 74-75 0.23997300581969938 1103 76-77 0.10884681882839686 1103 78-79 0.13891908246480256 1103 80-81 0.1656644535503844 1103 82-83 0.222833901117653 1103 84-85 0.14167728275192815 1103 86-87 0.1665558804454932 1103 88-89 0.20235066497021847 1103 90-91 0.18789014870766607 1103 92-93 0.16012865535920184 1103 94-95 0.1618382277719519 1103 96-97 0.12138443491316053 1103 98-99 0.09784699192321966 1103 100-101 0.21746531250074952 1103 102-103 0.28182074876140106 1103 104-105 0.263408522638116 1103 106-107 0.2794275471348442 1103 108-109 0.26737294579647397 1103 110-111 0.22785075964526413 1103 112-113 0.2555070298022031 1103 114-115 0.21394560591075873 1103 116-117 0.15639687838479688 1103 118-119 0.15908086566767565 1103 120-121 0.21014526843863024 1103 122-123 0.08514641830976544 1103 124-125 -0.01945110851450238 1104 1 0.17020118199967982 1104 2 0.16856890978758798 1104 3 0.1838402258243761 1104 4 -0.023615041550911542 1104 5 0.08867236996688632 1104 6 0.13019244420016207 1104 7 0.1762046518855982 1104 8 0.12372179024488617 1104 9 0.18358361318146876 1104 10-11 0.18800563653440605 1104 12-13 0.19073736267802843 1104 14-15 0.2770591942547611 1104 16-17 0.22613883037897153 1104 18-19 0.24006051925357497 1104 20-21 0.24209680513854437 1104 22-23 0.2192568050848891 1104 24-25 0.25815637915849265 1104 26-27 0.23566348842172857 1104 28-29 0.34696852898417774 1104 30-31 0.29883883916332366 1104 32-33 0.31251367760582127 1104 34-35 0.2617808205812011 1104 36-37 0.4254591250806783 1104 38-39 0.3626201578589914 1104 40-41 0.48126319342183166 1104 42-43 0.400543736697351 1104 44-45 0.39932542385444236 1104 46-47 0.49027358022900813 1104 48-49 0.3372730048711787 1104 50-51 0.3402008575602693 1104 52-53 0.32732203903368173 1104 54-55 0.3266760206162047 1104 56-57 0.3207971464802313 1104 58-59 0.28593149875715085 1104 60-61 0.31305353190376195 1104 62-63 0.3204747755323396 1104 64-65 0.30295120318304214 1104 66-67 0.24567467066335524 1104 68-69 0.1892380876158839 1104 70-71 0.2604768222714 1104 72-73 0.3542778567432663 1104 74-75 0.31591934965143764 1104 76-77 0.4326083887333354 1104 78-79 0.3865603544439864 1104 80-81 0.39615306878630463 1104 82-83 0.4404683715476878 1104 84-85 0.4232793160571475 1104 86-87 0.4223220950925466 1104 88-89 0.4860530121758728 1104 90-91 0.4753025843558447 1104 92-93 0.46217169305634087 1104 94-95 0.42699908217181104 1104 96-97 0.465499193156127 1104 98-99 0.51927903124691 1104 100-101 0.5356323845009641 1104 102-103 0.5050631412063176 1104 104-105 0.5226034787524583 1104 106-107 0.5465265074375196 1104 108-109 0.4682932804147981 1104 110-111 0.4514483316712017 1104 112-113 0.4919313390758049 1104 114-115 0.4650217720743868 1104 116-117 0.6023094605795052 1104 118-119 0.5372783209038587 1104 120-121 0.4849300911396952 1104 122-123 0.5162399015754922 1104 124-125 0.602457710454658 1105 1 0.0925255029667511 1105 2 0.13831194130307978 1105 3 0.09360868940715505 1105 4 0.023794951922177177 1105 5 -0.01778600218467119 1105 6 0.11378581878705063 1105 7 0.010003803932100652 1105 8 0.05152370342499779 1105 9 0.10072563286244218 1105 10-11 0.16138041956094895 1105 12-13 0.2062281503638559 1105 14-15 0.20250804191188365 1105 16-17 0.16549577173158525 1105 18-19 0.17191042429193004 1105 20-21 0.1552333287035168 1105 22-23 0.1834704867525616 1105 24-25 0.23987612736577546 1105 26-27 0.1955731141432011 1105 28-29 0.23504182651212346 1105 30-31 0.20208802003065074 1105 32-33 0.23473829702376037 1105 34-35 0.3004454082254071 1105 36-37 0.2818224266293754 1105 38-39 0.3055380244855641 1105 40-41 0.36722586908452115 1105 42-43 0.3737528952925473 1105 44-45 0.4678650631200796 1105 46-47 0.5073595927009578 1105 48-49 0.46257987316379 1105 50-51 0.48206254154667505 1105 52-53 0.48587147411271303 1105 54-55 0.6137396628935932 1105 56-57 0.4610608815944701 1105 58-59 0.4347848219786812 1105 60-61 0.414341588747817 1105 62-63 0.3659484886468505 1105 64-65 0.29229130968390393 1105 66-67 0.2760754708908735 1105 68-69 0.33421885012025143 1105 70-71 0.39360944494517014 1105 72-73 0.3403877420156647 1105 74-75 0.30673809005584474 1105 76-77 0.14319222575018387 1105 78-79 0.0634641364560764 1105 80-81 0.10034020695957224 1105 82-83 0.17749927031084667 1105 84-85 0.23455844879531895 1105 86-87 0.17511455569408696 1105 88-89 0.1732200607593981 1105 90-91 0.20381962460366765 1105 92-93 0.1835745101971682 1105 94-95 0.18450748052908494 1105 96-97 0.1860112341612492 1105 98-99 0.16556027242768323 1105 100-101 0.18183571682093458 1105 102-103 0.23327453900023798 1105 104-105 0.2603153990260907 1105 106-107 0.2300078676063606 1105 108-109 0.2088355260859487 1105 110-111 0.24340715656586553 1105 112-113 0.2854695890575556 1105 114-115 0.28797742722253616 1105 116-117 0.3351000470098455 1105 118-119 0.2999984406764753 1105 120-121 0.31237776417092533 1105 122-123 0.44726495336037786 1105 124-125 0.4180343613325235 1106 1 0.16303942609807365 1106 2 0.14827887744971235 1106 3 0.1933162749813775 1106 4 0.11354197203443306 1106 5 0.14204810518685207 1106 6 0.13121601200991506 1106 7 0.15511207371785218 1106 8 0.13464521149347064 1106 9 0.1442374090730354 1106 10-11 0.1940381417863577 1106 12-13 0.18106872398030305 1106 14-15 0.22467483917758102 1106 16-17 0.24275624991517475 1106 18-19 0.23589758099166858 1106 20-21 0.14738942321899628 1106 22-23 0.19021226379133083 1106 24-25 0.15717508448469175 1106 26-27 0.2195412224958062 1106 28-29 0.1826397598620204 1106 30-31 0.050957860572417246 1106 32-33 0.10699810628545947 1106 34-35 0.1386570933352047 1106 36-37 0.19713054479283443 1106 38-39 0.07972114344535441 1106 40-41 0.08525618080211927 1106 42-43 0.0815264047116031 1106 44-45 0.044784709674495105 1106 46-47 0.07297764467300993 1106 48-49 0.022524830448354294 1106 50-51 -0.1873737802748323 1106 52-53 -0.1443469129573458 1106 54-55 -0.1295042260271515 1106 56-57 -0.18335935424827454 1106 58-59 -0.14033192081767254 1106 60-61 -0.07871436497552509 1106 62-63 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-0.40787773732676413 2216 9 -0.5525905641862039 2216 10-11 -0.5483281904242645 2216 12-13 -0.5058726211015738 2216 14-15 -0.6520886533662633 2216 16-17 -0.7776890626564779 2216 18-19 -0.8229217485503781 2216 20-21 -0.7432275322484401 2216 22-23 -0.734172568960993 2216 24-25 -0.7059154046752809 2216 26-27 -0.7571663963684045 2216 28-29 -0.8866337774986661 2216 30-31 -0.845363057406594 2216 32-33 -0.8516629035805323 2216 34-35 -0.8667092035040369 2216 36-37 -0.9133532701807852 2216 38-39 -0.9280792283471513 2216 40-41 -0.9534139077180299 2216 42-43 -0.9325212732883088 2216 44-45 -0.9225046242824249 2216 46-47 -0.8133184347370488 2216 48-49 -0.6884366291581898 2216 50-51 -0.9440026388665999 2216 52-53 -0.883803621663553 2216 54-55 -0.8847326399875755 2216 56-57 -0.814886520123018 2216 58-59 -1.0424979593148294 2216 60-61 -1.002541443469866 2216 62-63 -0.9145001813286626 2216 64-65 -0.8122576554534646 2216 66-67 -0.7434483186760872 2216 68-69 -0.6707993006378103 2216 70-71 -0.6380700639565831 2216 72-73 -0.60938119088474 2216 74-75 -0.5994979609352526 2216 76-77 -0.5570342180117436 2216 78-79 -0.5353568791747847 2216 80-81 -0.6170188627317543 2216 82-83 -0.5798686336141969 2216 84-85 -0.6733140641649911 2216 86-87 -0.7313616427648455 2216 88-89 -0.6821689215940836 2216 90-91 -0.4786601873761711 2216 92-93 -0.4608113276214709 2216 94-95 -0.5013034628779351 2216 96-97 -0.510113743636758 2216 98-99 -0.4855094481697151 2216 100-101 -0.5659924786205224 2216 102-103 -0.5143035305467372 2216 104-105 -0.5826956776927119 2216 106-107 -0.6443081944009066 2216 108-109 -0.6266172250660631 2216 110-111 -0.7019110654381606 2216 112-113 -0.601353765516528 2216 114-115 -0.6036631791198879 2216 116-117 -0.7529871750826516 2216 118-119 -0.7293660732444316 2216 120-121 -0.7426213277922571 2216 122-123 -0.7681360164096525 2216 124-125 -0.9228855697764189 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 4.0 4 19.0 5 44.0 6 98.0 7 252.0 8 386.0 9 682.0 10 1330.0 11 1881.0 12 2463.0 13 4024.0 14 7399.0 15 7508.0 16 3255.0 17 2345.0 18 2051.0 19 1747.0 20 1567.0 21 1421.0 22 1425.0 23 1572.0 24 1823.0 25 2334.0 26 2841.0 27 3470.0 28 4238.0 29 5427.0 30 6522.0 31 8162.0 32 10409.0 33 14033.0 34 19981.0 35 35092.0 36 77122.0 37 277963.0 38 231258.0 39 2610.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.24826893428665 19.080534172768147 14.28337371143712 22.387823181508082 2 15.15571017886563 20.162900520846623 41.98609195849877 22.69529734178898 3 19.19018315942967 25.692806148549668 29.594057690990798 25.52295300102986 4 12.037810182664058 16.959856934443973 38.52793072615182 32.47440215674015 5 12.826819004227072 38.282065368502074 34.30724780956551 14.583867817705345 6 30.46120253215553 37.54011887272347 17.76349538448316 14.235183210637848 7 27.193177973156597 33.72128620610019 21.024781004452418 18.060754816290796 8 25.538022783056107 35.91392709636878 19.685430552497166 18.86261956807794 9 26.546932988168066 16.595726968543055 20.34362563174375 36.51371441154513 10-11 24.56073333297528 27.602905081341206 27.16048305234947 20.67587853333405 12-13 25.75809249641497 24.833370033997344 28.18483439049149 21.223703079096197 14-15 22.4993420734622 25.74164433296991 27.302272934782025 24.45674065878587 16-17 21.16596666863597 29.16903878275534 27.673934293279483 21.991060255329206 18-19 20.778729906386452 27.065822193339102 31.412903953467136 20.74254394680731 20-21 22.117677546175702 26.032676122906047 31.47352718445038 20.376119146467875 22-23 21.445719717924067 26.279599980665015 31.349796714126892 20.924883587284025 24-25 21.08426293429865 26.320015467987172 31.607730771089905 20.987990826624273 26-27 21.010749714003364 26.52867216997599 31.44103366972625 21.019544446294397 28-29 20.862570861563828 26.60816475598916 31.527454408253913 21.001809974193097 30-31 21.278864067404037 26.367533274030315 31.374767123747503 20.978835534818145 32-33 21.003057348445076 26.363151767743563 31.466117409162376 21.167673474648982 34-35 21.33155558061954 26.521613324014904 31.293415802798346 20.853415292567206 36-37 21.285130586218052 26.458898674332705 31.089737995077588 21.166232744371655 38-39 21.216389502447146 26.50773083404387 31.18295523971292 21.092924423796063 40-41 21.563556753251202 26.33057339678972 30.933108295452183 21.1727615545069 42-43 21.328864755705222 26.46047833115812 30.842987501946943 21.367669411189706 44-45 21.517818839504564 26.637928082824054 30.516092778944078 21.32816029872731 46-47 21.626879960470646 26.90097386685429 29.802232388021427 21.66991378465364 48-49 21.642051073411736 27.034252364714938 29.654992302801674 21.66870425907165 50-51 21.848726519821067 27.24167355376062 29.25603943871135 21.653560487706958 52-53 21.860347707461692 27.40586279680543 29.070752614250956 21.66303688148192 54-55 21.722921623494155 27.25615094177484 29.19387886632544 21.827048568405562 56-57 21.76378690724674 27.434507029488202 29.024693338954865 21.777012724310193 58-59 21.882569674574487 27.235856961012317 29.208403379398135 21.673169985015065 60-61 21.900797039567323 27.186219379232927 29.27918674035523 21.633796840844518 62-63 21.79094437361659 27.220654610490445 29.41732509716172 21.571075918731246 64-65 21.73831896169742 27.148494670723487 29.314981859781884 21.798204507797205 66-67 21.77588872649493 27.353259676445884 29.095969907063957 21.774881689995226 68-69 21.881201678934634 27.145059200438265 29.111939717330998 21.861799403296104 70-71 21.804389625595498 27.06899977979365 29.213259107680905 21.913351486929947 72-73 21.779315326734373 27.14611608509541 29.11439059890113 21.960177989269084 74-75 21.703817187481118 27.320436165598988 28.89482963036563 22.08091701655426 76-77 21.74555815752159 27.248783461521935 28.961739462037556 22.04391891891892 78-79 21.758194099161006 27.22110590211458 28.852574357996584 22.168125640727833 80-81 21.603756357718687 27.336511968892157 28.842828063655062 22.21690360973409 82-83 21.796837655955446 27.333222336203143 28.803352471386912 22.066587536454502 84-85 21.957417268872472 27.065223521071164 28.71709351443817 22.260265695618198 86-87 21.6744700648933 27.224908393430912 28.834820835140402 22.265800706535384 88-89 21.710165904904105 27.045010231429018 28.878141263541202 22.366682600125678 90-91 21.708756062323097 27.254069745583838 28.777505357401807 22.259668834691258 92-93 21.68743290610312 27.142086998671385 28.862913279942426 22.307566815283074 94-95 21.631105922255337 27.22761808022332 28.804840763818067 22.336435233703273 96-97 21.556416680765132 27.135917246046386 28.799468823597334 22.508197249591145 98-99 21.4804286995612 27.085895666278176 28.680091948590423 22.753583685570195 100-101 21.49738070158125 27.06883622318853 28.728698689041643 22.705084386188577 102-103 21.633698728724802 26.916781691918512 28.78066071936882 22.66885885998786 104-105 21.43833482821404 26.892411555875416 28.79066388815786 22.878589727752686 106-107 21.346215300995485 26.899130191927085 28.837736082130938 22.91691842494649 108-109 21.413758401202 26.857864854359025 28.64884570119616 23.079531043242817 110-111 21.342935480232665 27.025943788904943 28.603490501689127 23.02763022917327 112-113 21.330961212467063 27.04036521709941 28.4952585555798 23.13341501485373 114-115 21.14303323345595 27.00878755388579 28.549647430367248 23.298531782291022 116-117 21.07515389216773 26.825407162187474 28.81873788617384 23.280701059470957 118-119 20.96616100176969 26.890201164935913 28.679229069152296 23.464408764142103 120-121 20.94420767976884 27.01648311809679 28.650763599646602 23.388545602487774 122-123 20.962726247381706 26.968419356571243 28.570747623395455 23.498106772651592 124-125 20.747237018248903 26.931401786085928 28.603222259072425 23.71813893659274 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 268.0 1 190.0 2 251.5 3 386.5 4 232.5 5 136.0 6 391.0 7 1073.5 8 1972.0 9 2144.5 10 1457.0 11 861.0 12 739.5 13 816.0 14 898.5 15 972.5 16 1041.0 17 1128.5 18 1254.5 19 1372.0 20 1478.5 21 1693.0 22 2006.5 23 2456.5 24 3078.5 25 3735.5 26 4681.5 27 5869.5 28 7002.5 29 8460.5 30 10236.0 31 11858.0 32 13162.0 33 14486.5 34 16233.0 35 17927.5 36 19846.5 37 21309.0 38 21809.5 39 22586.5 40 23602.5 41 24583.5 42 25552.5 43 26443.5 44 27555.5 45 28565.5 46 28704.0 47 28534.0 48 28592.0 49 28541.5 50 27785.0 51 26648.0 52 25259.0 53 23957.5 54 22454.5 55 20421.5 56 18151.5 57 15864.5 58 14006.0 59 12048.0 60 9959.5 61 7989.0 62 6102.5 63 4480.0 64 3263.5 65 2282.5 66 1653.0 67 1280.0 68 914.0 69 605.5 70 437.0 71 322.5 72 238.5 73 208.0 74 152.5 75 95.5 76 63.5 77 33.5 78 22.0 79 11.5 80 5.5 81 1.5 82 1.0 83 1.0 84 0.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018260818192071582 2 0.002014060830007895 3 4.02812166001579E-4 4 0.14084998737855212 5 0.037192989994145796 6 0.013695613644053686 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.685414440010527E-4 30-31 2.014060830007895E-4 32-33 1.3427072200052634E-4 34-35 4.699475270018422E-4 36-37 9.398950540036844E-4 38-39 0.0025511437180100005 40-41 6.713536100026317E-5 42-43 0.0010741657760042107 44-45 6.042182490023685E-4 46-47 1.3427072200052634E-4 48-49 0.0016783840250065793 50-51 0.0018126547470071056 52-53 0.0 54-55 0.0 56-57 0.001141301137004474 58-59 0.0021483315520084215 60-61 0.0010741657760042107 62-63 6.713536100026317E-5 64-65 0.0013427072200052634 66-67 6.042182490023685E-4 68-69 8.05624332003158E-4 70-71 0.0010741657760042107 72-73 0.0 74-75 1.3427072200052634E-4 76-77 0.0037595802160147376 78-79 7.384889710028949E-4 80-81 0.0 82-83 0.0 84-85 2.685414440010527E-4 86-87 4.02812166001579E-4 88-89 0.0 90-91 0.0 92-93 7.384889710028949E-4 94-95 7.384889710028949E-4 96-97 2.685414440010527E-4 98-99 0.0 100-101 4.699475270018422E-4 102-103 0.0 104-105 0.0 106-107 2.685414440010527E-4 108-109 6.042182490023685E-4 110-111 0.0 112-113 2.014060830007895E-4 114-115 0.002349737635009211 116-117 7.384889710028949E-4 118-119 2.685414440010527E-4 120-121 2.685414440010527E-4 122-123 5.370828880021054E-4 124-125 9.398950540036844E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 744764.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.54645773372219 #Duplication Level Percentage of deduplicated Percentage of total 1 79.17574861811913 53.48041357569596 2 13.020330874898805 17.589544582408603 3 3.9324431976335394 7.968678247176515 4 1.571158268156297 4.245047022120298 5 0.742865371118684 2.5088962196057016 6 0.4288398883929732 1.7379969237522064 7 0.27903324088232034 1.3193394908092821 8 0.16064006011999515 0.8680533624990275 9 0.12757079202293117 0.7755259600290945 >10 0.5333406181539545 5.97814263000387 >50 0.018487611572076807 0.8334465884632108 >100 0.00854755696283689 1.05804062459881 >500 1.987803932851984E-4 0.09116872863231971 >1k 7.951215731407936E-4 1.5457060442051025 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4730 0.6351005150624897 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3468 0.46565086389782534 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2281 0.3062715168832006 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1033 0.13870165582654373 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 1.3427072200052634E-4 0.0 0.0 0.0 0.0 12-13 1.3427072200052634E-4 0.0 0.0 0.0 0.0 14-15 3.3567680500131585E-4 0.0 0.0 0.0 0.0 16-17 5.370828880021054E-4 0.0 0.0 0.0 0.0 18-19 5.370828880021054E-4 0.0 0.0 0.0 0.0 20-21 5.370828880021054E-4 0.0 0.0 0.0 0.0 22-23 6.713536100026317E-4 0.0 0.0 6.713536100026317E-5 0.0 24-25 7.384889710028949E-4 0.0 0.0 1.3427072200052634E-4 0.0 26-27 8.05624332003158E-4 0.0 0.0 4.699475270018422E-4 0.0 28-29 8.05624332003158E-4 0.0 0.0 9.398950540036844E-4 0.0 30-31 8.05624332003158E-4 0.0 0.0 0.002484008357009737 0.0 32-33 8.05624332003158E-4 0.0 0.0 0.007989107959031317 0.0 34-35 8.05624332003158E-4 0.0 0.0 0.014971185503058686 0.0 36-37 8.05624332003158E-4 0.0 0.0 0.025310031097099214 0.0 38-39 8.05624332003158E-4 0.0 0.0 0.04276522495716764 0.0 40-41 8.05624332003158E-4 0.0 0.0 0.06948509863527239 0.0 42-43 8.05624332003158E-4 0.0 0.0 0.09358669323436686 0.0 44-45 9.398950540036844E-4 0.0 0.0 0.12131359732747554 0.0 46-47 0.0010741657760042107 0.0 0.0 0.15702960937961555 0.0 48-49 0.0010741657760042107 1.3427072200052634E-4 0.0 0.19623666020376926 0.0 50-51 0.0010741657760042107 1.3427072200052634E-4 0.0 0.23739063649693057 0.0 52-53 0.001141301137004474 1.3427072200052634E-4 0.0 0.28364690022611194 0.0 54-55 0.001208436498004737 1.3427072200052634E-4 0.0 0.3291646749842903 0.0 56-57 0.001208436498004737 1.3427072200052634E-4 0.0 0.38260442234049985 0.0 58-59 0.001208436498004737 1.3427072200052634E-4 0.0 0.44819567003775695 0.0 60-61 0.001208436498004737 1.3427072200052634E-4 0.0 0.5229173268310499 0.0 62-63 0.001208436498004737 1.3427072200052634E-4 0.0 0.5960277349603365 0.0 64-65 0.001208436498004737 1.3427072200052634E-4 0.0 0.6682653833966196 0.0 66-67 0.001208436498004737 1.3427072200052634E-4 0.0 0.7549371344479594 0.0 68-69 0.001208436498004737 1.3427072200052634E-4 0.0 0.8559758527533554 0.0 70-71 0.001208436498004737 1.3427072200052634E-4 0.0 0.97191862120081 0.0 72-73 0.001208436498004737 1.3427072200052634E-4 0.0 1.0882642018142659 0.0 74-75 0.001208436498004737 1.3427072200052634E-4 0.0 1.1994403596307017 0.0 76-77 0.001208436498004737 1.3427072200052634E-4 0.0 1.3214253105681801 0.0 78-79 0.001208436498004737 1.3427072200052634E-4 0.0 1.4740911214827785 0.0 80-81 0.0013427072200052634 1.3427072200052634E-4 0.0 1.6534768060754816 0.0 82-83 0.0014098425810055266 1.3427072200052634E-4 0.0 1.825007653431154 0.0 84-85 0.0014769779420057898 1.3427072200052634E-4 0.0 1.999760998114839 0.0 86-87 0.0014769779420057898 1.3427072200052634E-4 0.0 2.1874043321105745 0.0 88-89 0.001611248664006316 1.3427072200052634E-4 0.0 2.4150603412624667 0.0 90-91 0.0017455193860068424 1.3427072200052634E-4 0.0 2.667355027901456 0.0 92-93 0.0017455193860068424 1.3427072200052634E-4 0.0 2.928108770026478 0.0 94-95 0.0017455193860068424 1.3427072200052634E-4 0.0 3.194367611753522 0.0 96-97 0.0017455193860068424 1.3427072200052634E-4 0.0 3.4606935888415657 0.0 98-99 0.0017455193860068424 1.3427072200052634E-4 0.0 3.7566933954917263 0.0 100-101 0.0017455193860068424 1.3427072200052634E-4 0.0 4.095659833182054 0.0 102-103 0.0017455193860068424 1.3427072200052634E-4 0.0 4.464232965073499 0.0 104-105 0.0018126547470071056 1.3427072200052634E-4 0.0 4.826293966947919 0.0 106-107 0.002014060830007895 1.3427072200052634E-4 0.0 5.18875778098834 0.0 108-109 0.002014060830007895 1.3427072200052634E-4 0.0 5.570489443635836 0.0 110-111 0.002014060830007895 1.3427072200052634E-4 0.0 6.005258041473541 0.0 112-113 0.002014060830007895 1.3427072200052634E-4 0.0 6.470103281039362 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1915 0.0 69.691376 1 GTATCAA 3920 0.0 59.427914 1 TAACGCA 120 7.2759576E-12 49.646584 4 ATCAACG 4855 0.0 47.85706 3 TCAACGC 4950 0.0 46.938587 4 TATCAAC 4975 0.0 46.822468 2 CAACGCA 5030 0.0 46.276268 5 AACGCAG 5275 0.0 44.219006 6 CTATACG 60 0.0013000411 39.717266 4 TGGTATC 830 0.0 38.760227 2 ATAACGC 155 1.200533E-10 38.436066 3 ACGCAGA 6045 0.0 38.384453 7 GTGGTAT 915 0.0 37.766495 1 CGCAGAG 6115 0.0 37.75047 8 GCAGAGT 6690 0.0 34.150116 9 CCTAGCG 75 0.003885284 31.773811 3 GTACATG 4060 0.0 30.96394 1 TACATGG 4045 0.0 30.487541 2 TATAACG 160 2.5719964E-7 29.78795 2 ACATGGG 4045 0.0 29.456562 3 >>END_MODULE