FastQCFastQC Report
Fri 27 May 2016
SRR940034_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940034_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639904
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23380.36536730509576437No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8500.056.1276631
TAGATCG453.1441395E-452.9762345
GTATCAA14100.046.5242581
ATCAACG15350.038.0586553
TCAACGC15850.036.866734
CAACGCA15850.036.8494035
AACGCAG16350.034.6289636
TATCAAC18250.034.3162962
GACCGTA700.002765597534.0561527
TACATGG32850.032.8637542
GTACATG34600.032.7478941
GAGCGTA750.003894128431.758389
GTACCGT850.007150173728.0462446
TGACGCA850.007150173728.0462445
CGCAGAG20400.027.7540958
ACATGGG35950.027.6919353
ACGCAGA20450.027.6862377
GTATAGG2701.6643753E-924.2960011
GTGTAGC6000.023.8542581
GTAGCCC4500.023.8449123
Produced by FastQC (version 0.11.5)