##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940034_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 639904 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.8396743886583 33.0 31.0 34.0 26.0 34.0 2 30.819516677501625 34.0 31.0 34.0 26.0 34.0 3 31.087217770165523 34.0 31.0 34.0 27.0 34.0 4 34.15424344901735 37.0 35.0 37.0 30.0 37.0 5 34.4397300220033 37.0 35.0 37.0 31.0 37.0 6 34.31358922588388 37.0 35.0 37.0 31.0 37.0 7 34.35850065009751 37.0 35.0 37.0 31.0 37.0 8 34.25076573986098 37.0 36.0 37.0 30.0 37.0 9 35.74383657548632 39.0 38.0 39.0 30.0 39.0 10-11 35.829486141671254 39.0 38.0 39.0 30.0 39.0 12-13 35.7514775653848 39.0 38.0 39.0 30.0 39.0 14-15 37.15975130644597 41.0 39.0 41.0 30.0 41.0 16-17 37.21068863454518 41.0 39.0 41.0 30.0 41.0 18-19 37.22565806746012 41.0 39.0 41.0 30.0 41.0 20-21 37.264521709506425 41.0 39.0 41.0 30.0 41.0 22-23 37.3096855153273 41.0 39.0 41.0 30.0 41.0 24-25 37.4158772253338 41.0 39.0 41.0 30.0 41.0 26-27 37.4790374806221 41.0 39.0 41.0 30.0 41.0 28-29 37.44522928439266 41.0 38.0 41.0 30.0 41.0 30-31 37.39620552457869 41.0 38.0 41.0 30.0 41.0 32-33 37.348264739710956 41.0 38.0 41.0 30.0 41.0 34-35 37.257615986147925 41.0 38.0 41.0 30.0 41.0 36-37 37.10475946391959 41.0 38.0 41.0 30.0 41.0 38-39 36.990356365954895 40.0 38.0 41.0 30.0 41.0 40-41 36.8982683340001 40.0 38.0 41.0 30.0 41.0 42-43 36.716264470920635 40.0 37.5 41.0 29.0 41.0 44-45 36.54879403785568 40.0 37.0 41.0 29.0 41.0 46-47 36.38559065109766 40.0 37.0 41.0 28.5 41.0 48-49 36.23728324873731 40.0 36.5 41.0 28.0 41.0 50-51 36.07344070360554 40.0 36.0 41.0 28.0 41.0 52-53 36.225958893834076 40.0 36.0 41.0 29.0 41.0 54-55 36.053686177926686 40.0 36.0 41.0 28.5 41.0 56-57 35.93815791118668 40.0 35.5 41.0 29.0 41.0 58-59 35.75404904485673 40.0 35.0 41.0 28.0 41.0 60-61 35.519835006500976 39.0 35.0 41.0 28.0 41.0 62-63 35.24224805595839 39.0 35.0 41.0 28.0 41.0 64-65 34.94298597914687 38.5 35.0 41.0 27.0 41.0 66-67 34.62778713682053 37.5 35.0 40.0 26.5 41.0 68-69 34.28828621168175 37.0 34.5 40.0 26.0 41.0 70-71 33.92741801895284 36.5 34.0 39.5 26.0 41.0 72-73 33.55661474221133 36.0 34.0 39.0 26.0 41.0 74-75 33.17488013827074 35.5 34.0 39.0 25.0 40.5 76-77 32.79612019927989 35.0 34.0 37.5 25.0 39.5 78-79 32.43377209706456 35.0 33.5 37.0 24.0 39.0 80-81 32.09379688203231 35.0 33.0 36.5 23.0 39.0 82-83 31.7577050620093 35.0 33.0 36.0 21.0 37.5 84-85 31.48853499899985 35.0 33.0 36.0 20.0 37.0 86-87 31.24905845251788 35.0 33.0 35.5 20.0 37.0 88-89 31.057496905785868 35.0 33.0 35.0 18.5 36.0 90-91 30.882330787118068 35.0 33.0 35.0 18.0 36.0 92-93 30.718946904535677 35.0 32.5 35.0 16.5 36.0 94-95 30.587686590488573 35.0 32.0 35.0 14.0 36.0 96-97 30.465322142071308 35.0 32.0 35.0 10.0 35.0 98-99 30.390276510226535 35.0 32.0 35.0 8.5 35.0 100-101 30.28858860079012 35.0 32.0 35.0 7.0 35.0 102-103 29.55985819747962 34.0 30.0 34.5 7.0 35.0 104-105 30.467974102365353 35.0 32.5 35.0 7.0 35.0 106-107 30.57823907961194 35.0 33.0 35.0 7.0 35.0 108-109 30.566424026103917 35.0 33.0 35.0 2.0 35.0 110-111 30.51238623293494 35.0 33.0 35.0 2.0 35.0 112-113 30.447435084012604 35.0 33.0 35.0 2.0 35.0 114-115 30.382747255838375 35.0 33.0 35.0 2.0 35.0 116-117 30.299262076811523 35.0 33.0 35.0 2.0 35.0 118-119 30.207095908136218 35.0 33.0 35.0 2.0 35.0 120-121 30.10879210006501 35.0 33.0 35.0 2.0 35.0 122-123 29.966230090763617 35.0 32.5 35.0 2.0 35.0 124-125 28.714409505175777 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14029844400165103 1101 2 0.0929831454437604 1101 3 0.32401406349250905 1101 4 0.39815111033038164 1101 5 0.25058057732799455 1101 6 0.370391002348768 1101 7 0.38785053861179364 1101 8 0.44718754825657214 1101 9 0.3218328611411536 1101 10-11 0.17513807558300698 1101 12-13 0.1626963902285823 1101 14-15 0.2767483629334677 1101 16-17 0.28594002989178335 1101 18-19 0.2882394196085656 1101 20-21 0.08456870780099024 1101 22-23 0.20403664172596336 1101 24-25 0.33516299787966375 1101 26-27 0.4205092771415764 1101 28-29 0.4877856745363758 1101 30-31 0.4522014686229028 1101 32-33 0.4725704656980696 1101 34-35 0.38592971219556205 1101 36-37 0.45632545179045536 1101 38-39 0.4413825697898943 1101 40-41 0.4225461285903762 1101 42-43 0.35173070972787457 1101 44-45 0.48722067849534056 1101 46-47 0.5080906066850019 1101 48-49 0.4310964644528852 1101 50-51 0.6178571164917059 1101 52-53 0.5220392919343269 1101 54-55 0.5519829461194092 1101 56-57 0.5289705602781822 1101 58-59 0.4961858966329942 1101 60-61 0.3942688068336153 1101 62-63 0.45732227510169565 1101 64-65 0.29675271500924083 1101 66-67 0.2230336962583408 1101 68-69 0.2789156861841704 1101 70-71 0.320552288651605 1101 72-73 0.33711277827545416 1101 74-75 0.32358180153826055 1101 76-77 0.24582970193399234 1101 78-79 0.33525263924961024 1101 80-81 0.46156823692921733 1101 82-83 0.4130875689243041 1101 84-85 0.34488847722674976 1101 86-87 0.3392722763434044 1101 88-89 0.4058434946055769 1101 90-91 0.3111028173177246 1101 92-93 0.3704894737081972 1101 94-95 0.4021063278173109 1101 96-97 0.38337247630425964 1101 98-99 0.2913606306216714 1101 100-101 0.3579792964013464 1101 102-103 0.2996437152049083 1101 104-105 0.35669283341285407 1101 106-107 0.3369073385839805 1101 108-109 0.3566111621771455 1101 110-111 0.4022608809920918 1101 112-113 0.4282966678337452 1101 114-115 0.4364970371781851 1101 116-117 0.3811278606730859 1101 118-119 0.4678205043966308 1101 120-121 0.5336432263227842 1101 122-123 0.5517474266800306 1101 124-125 0.41478277489748194 1102 1 -0.1414519667489813 1102 2 -0.17520023325606005 1102 3 -0.03528238811248485 1102 4 -0.17037680845134417 1102 5 -0.024990182131531924 1102 6 0.09367381948504061 1102 7 -0.03696370401308258 1102 8 0.09606319503554772 1102 9 0.19320660743213125 1102 10-11 0.17830146850584327 1102 12-13 0.12680782013122638 1102 14-15 0.23363487314706077 1102 16-17 0.2655435022672705 1102 18-19 0.11581304819190308 1102 20-21 0.05188951017476029 1102 22-23 -0.015203040463397599 1102 24-25 0.0650116945127337 1102 26-27 0.048194482762113466 1102 28-29 0.0035658555118729396 1102 30-31 0.05648614946353092 1102 32-33 0.202805390536831 1102 34-35 0.2260343003414249 1102 36-37 0.2182065670703821 1102 38-39 0.2130787859796044 1102 40-41 0.18133741822474292 1102 42-43 0.04241709242484859 1102 44-45 0.10401792707916258 1102 46-47 0.09470381446351439 1102 48-49 -0.034170584763622 1102 50-51 -0.09714325134467572 1102 52-53 -0.07419896795434511 1102 54-55 0.020747506309696462 1102 56-57 0.007231429843400861 1102 58-59 -0.0432768170186435 1102 60-61 0.017791330681667716 1102 62-63 -0.01784876881797004 1102 64-65 -0.13579835294239473 1102 66-67 -0.029197844485665314 1102 68-69 -0.004330592537719724 1102 70-71 -0.031856426618510625 1102 72-73 -0.0012108686997081008 1102 74-75 -0.17803361325857026 1102 76-77 -0.11136701696543838 1102 78-79 -0.02935598283527696 1102 80-81 -0.04259137954004899 1102 82-83 -0.07321359098643399 1102 84-85 -0.09500399193630216 1102 86-87 -0.14429156779205954 1102 88-89 -0.29573697573454893 1102 90-91 -0.15207676040610352 1102 92-93 -0.15996124847724502 1102 94-95 -0.30532580636940665 1102 96-97 -0.2528158804479901 1102 98-99 -0.26912393251131306 1102 100-101 -0.1941768378834574 1102 102-103 -0.1667452349900458 1102 104-105 -0.24336785959089724 1102 106-107 -0.2964609392060602 1102 108-109 -0.2741056283277672 1102 110-111 -0.2592744680846373 1102 112-113 -0.17904718562023803 1102 114-115 -0.16627644917508633 1102 116-117 -0.2830252573533514 1102 118-119 -0.3211793484688137 1102 120-121 -0.29406834090544365 1102 122-123 -0.20353102546445712 1102 124-125 -0.11671934629234926 1103 1 0.45965224305087915 1103 2 0.5084675482378067 1103 3 0.48789009158173613 1103 4 0.5292568911823565 1103 5 0.591442901692858 1103 6 0.4878581375823998 1103 7 0.6100076820887352 1103 8 0.43953614720415146 1103 9 0.5456247207198146 1103 10-11 0.5777082211734594 1103 12-13 0.6679104373208062 1103 14-15 0.8932276957833594 1103 16-17 0.8650750094074766 1103 18-19 0.8145110892812468 1103 20-21 0.6162442375729711 1103 22-23 0.7247037918347345 1103 24-25 0.8470297971830121 1103 26-27 0.8814349138286346 1103 28-29 0.8886722949661348 1103 30-31 0.9097801984097558 1103 32-33 0.9736287700558677 1103 34-35 1.0371905354107014 1103 36-37 0.9804696662899985 1103 38-39 1.0595113389338948 1103 40-41 0.9806552501512655 1103 42-43 1.0609046435210914 1103 44-45 1.0077778145347054 1103 46-47 0.981356413072632 1103 48-49 1.0611338692364782 1103 50-51 0.9130759980050058 1103 52-53 0.969803527135106 1103 54-55 1.0152994519486 1103 56-57 0.878787410095029 1103 58-59 0.9924640122004433 1103 60-61 0.8935080053113964 1103 62-63 0.9319043765873545 1103 64-65 0.9483180137826608 1103 66-67 1.0124024634189794 1103 68-69 0.9068349327582439 1103 70-71 0.9380697824381343 1103 72-73 0.9753578524342288 1103 74-75 0.9912874038885278 1103 76-77 0.784595715953813 1103 78-79 0.8134799252180187 1103 80-81 0.8336296216809487 1103 82-83 0.9911743157927759 1103 84-85 0.6774385413757962 1103 86-87 0.7984872717259144 1103 88-89 0.8452156706528804 1103 90-91 0.6251170602659784 1103 92-93 0.6809104402205293 1103 94-95 0.8005230549142404 1103 96-97 0.6800091447683414 1103 98-99 0.8206793801180865 1103 100-101 0.8745774754360731 1103 102-103 0.8144553277322579 1103 104-105 0.9399084052071061 1103 106-107 0.8306559053020912 1103 108-109 0.831146004097775 1103 110-111 0.8955670671916813 1103 112-113 0.8441452017025846 1103 114-115 0.9106731712527925 1103 116-117 0.9560520904196501 1103 118-119 0.8420101190684797 1103 120-121 0.7260516357362725 1103 122-123 0.990621440427141 1103 124-125 0.7629788540580265 1104 1 -0.06268294755934178 1104 2 0.049578762449144165 1104 3 -0.023283763379815525 1104 4 -0.07870008951286422 1104 5 -0.04118890826102728 1104 6 0.07868427967513014 1104 7 0.14600512520318887 1104 8 0.030481590984337004 1104 9 0.11967351069139909 1104 10-11 0.09565649726407344 1104 12-13 0.1456172232302393 1104 14-15 0.19936245366721295 1104 16-17 0.29207931457824543 1104 18-19 0.2981299365912946 1104 20-21 0.010446470134667152 1104 22-23 0.2527927113591204 1104 24-25 0.1687547817547994 1104 26-27 0.17841510827565799 1104 28-29 0.17547281518040592 1104 30-31 0.1234546756547985 1104 32-33 0.27628021323806706 1104 34-35 0.29534981347347866 1104 36-37 0.17675187222238975 1104 38-39 0.1434194560145059 1104 40-41 0.015925100258272096 1104 42-43 0.022159579444313238 1104 44-45 0.08027052755817721 1104 46-47 0.16042309093303686 1104 48-49 -0.1719610080079761 1104 50-51 0.01686699323146712 1104 52-53 -0.07862706653070717 1104 54-55 0.029655421672487137 1104 56-57 -0.11638529014400945 1104 58-59 0.004669605829441537 1104 60-61 -0.1710857577142164 1104 62-63 -0.10199939555526782 1104 64-65 -0.007114705220622852 1104 66-67 -0.023309981219874487 1104 68-69 -0.10542221098261706 1104 70-71 -0.1505897820959774 1104 72-73 -0.07309262484616141 1104 74-75 -0.05562025218679878 1104 76-77 -0.06637217421793906 1104 78-79 -0.0111657414059394 1104 80-81 -0.06619746451524833 1104 82-83 -0.08912750274788195 1104 84-85 0.06614995059383943 1104 86-87 0.04554412855357626 1104 88-89 0.08873692623686225 1104 90-91 0.1305231941401459 1104 92-93 0.11920053773861383 1104 94-95 0.15752230817404822 1104 96-97 0.10759375691266371 1104 98-99 0.04858831610905057 1104 100-101 0.130805097164302 1104 102-103 0.15364033519229636 1104 104-105 0.13029475120261935 1104 106-107 0.08859351788385439 1104 108-109 0.14833089667470745 1104 110-111 0.12702559659954815 1104 112-113 0.1963723874641481 1104 114-115 0.16276500114920722 1104 116-117 0.188586544305128 1104 118-119 0.08122620388167334 1104 120-121 0.07344563029552731 1104 122-123 0.15560168200569535 1104 124-125 0.04539393261303104 1105 1 0.26917605257468935 1105 2 0.476025677086259 1105 3 0.5364281723778817 1105 4 0.4437211604853317 1105 5 0.13766370597817712 1105 6 0.2718853409644453 1105 7 0.1352351428000702 1105 8 0.2844689921370005 1105 9 0.40033870446605135 1105 10-11 0.3585713246172091 1105 12-13 0.39781971561718166 1105 14-15 0.6097322187394951 1105 16-17 0.6275311912184947 1105 18-19 0.4918779279384111 1105 20-21 0.14304061920805822 1105 22-23 0.248854670824322 1105 24-25 0.3135507314458792 1105 26-27 0.3314395089945208 1105 28-29 0.4887484434293796 1105 30-31 0.5483047562891556 1105 32-33 0.5327882485701849 1105 34-35 0.2638635040191559 1105 36-37 0.4084174652055381 1105 38-39 0.53419197490485 1105 40-41 0.490607066554702 1105 42-43 0.3935027503127557 1105 44-45 0.269275576032463 1105 46-47 0.25280599097382606 1105 48-49 0.33075850982886124 1105 50-51 0.3116147352534213 1105 52-53 0.1850148391206119 1105 54-55 0.3223746945077153 1105 56-57 0.3300458290953827 1105 58-59 0.23298155916960184 1105 60-61 0.18958110783611204 1105 62-63 0.31447163012234114 1105 64-65 0.25859674341622707 1105 66-67 0.2753772425227936 1105 68-69 0.14053850517149158 1105 70-71 0.23592187061280612 1105 72-73 0.15015139819551848 1105 74-75 0.23991870090691947 1105 76-77 0.19831819419241725 1105 78-79 0.0013696937529488196 1105 80-81 0.04268341137990461 1105 82-83 0.1194582182702284 1105 84-85 0.08285689840705501 1105 86-87 0.14825816988390628 1105 88-89 0.20672393538695033 1105 90-91 0.3897132764750957 1105 92-93 0.30100181837642737 1105 94-95 0.3449845671176881 1105 96-97 0.3326277328707974 1105 98-99 0.36642097424677544 1105 100-101 0.3765402677214489 1105 102-103 0.29728910088861227 1105 104-105 0.4649914464191802 1105 106-107 0.42085244711153535 1105 108-109 0.3636469559535662 1105 110-111 0.4052234800739001 1105 112-113 0.4045913266648391 1105 114-115 0.3802925602270193 1105 116-117 0.3964199662713952 1105 118-119 0.40026369345109103 1105 120-121 0.5261487906721349 1105 122-123 0.5360019858076868 1105 124-125 0.5595377312620649 1106 1 -0.17843922024866998 1106 2 -0.005826586745982354 1106 3 0.01502579113122593 1106 4 0.02819631877814288 1106 5 -0.0982050114984574 1106 6 0.015740085202757825 1106 7 -0.006220454947964527 1106 8 0.07730820471461897 1106 9 0.07850246525035232 1106 10-11 0.30176775260516564 1106 12-13 0.032525938083750816 1106 14-15 0.29147807798142367 1106 16-17 0.36718018392644325 1106 18-19 0.337369654945995 1106 20-21 0.19739290790118957 1106 22-23 0.3143929374097283 1106 24-25 0.35935087549695766 1106 26-27 0.35186592290411056 1106 28-29 0.40444731133568723 1106 30-31 0.3349877032843267 1106 32-33 0.4124821613468299 1106 34-35 0.46564581642246594 1106 36-37 0.4533346926627573 1106 38-39 0.35669519616531176 1106 40-41 0.27925828537737374 1106 42-43 0.3482253951700258 1106 44-45 0.38294096555992496 1106 46-47 0.3548187282709776 1106 48-49 0.3653925646961298 1106 50-51 0.39475735007874846 1106 52-53 0.36430089131142296 1106 54-55 0.3762773563272219 1106 56-57 0.23170539975499338 1106 58-59 0.19479523890741746 1106 60-61 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32-33 -1.631190246053471 2216 34-35 -1.8878262041221632 2216 36-37 -1.9968138029289477 2216 38-39 -1.7295902404902677 2216 40-41 -1.6931700285127178 2216 42-43 -1.6285573281989443 2216 44-45 -1.6697498549284475 2216 46-47 -1.7331557772460116 2216 48-49 -1.6775620226852155 2216 50-51 -1.52927108143885 2216 52-53 -1.764293916639751 2216 54-55 -1.5934385842087906 2216 56-57 -1.6734103921027668 2216 58-59 -1.5602852099036184 2216 60-61 -1.5286728604178634 2216 62-63 -1.5198857687511804 2216 64-65 -1.6317855743854395 2216 66-67 -1.6608320082025827 2216 68-69 -1.863045724219191 2216 70-71 -2.010195214213958 2216 72-73 -1.8265937801625611 2216 74-75 -1.9035784829128417 2216 76-77 -1.8068021335085547 2216 78-79 -1.7872034415943787 2216 80-81 -1.7694859007894053 2216 82-83 -1.7285960572034007 2216 84-85 -1.7844839447402343 2216 86-87 -1.645691309549445 2216 88-89 -1.7362270801447117 2216 90-91 -1.8206468532398716 2216 92-93 -1.8611845615518874 2216 94-95 -1.9364201094482816 2216 96-97 -2.026989949327529 2216 98-99 -2.102259687982272 2216 100-101 -2.0667947723877838 2216 102-103 -2.0217926536770143 2216 104-105 -2.01853816614269 2216 106-107 -2.145071772038488 2216 108-109 -2.028527476543445 2216 110-111 -2.075276605264893 2216 112-113 -2.0791089634870623 2216 114-115 -2.0825423945434167 2216 116-117 -2.0952595012145245 2216 118-119 -2.1144572486892095 2216 120-121 -2.1779816070066467 2216 122-123 -2.144958929024199 2216 124-125 -2.076193622211221 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15205.0 3 2993.0 4 2331.0 5 1543.0 6 1652.0 7 1802.0 8 1921.0 9 2001.0 10 2320.0 11 2694.0 12 2923.0 13 2753.0 14 2806.0 15 2782.0 16 2633.0 17 2523.0 18 2179.0 19 1783.0 20 1677.0 21 1510.0 22 1617.0 23 2301.0 24 4638.0 25 6877.0 26 7277.0 27 5140.0 28 5233.0 29 6163.0 30 6910.0 31 8479.0 32 10647.0 33 14618.0 34 22093.0 35 39342.0 36 93291.0 37 223262.0 38 123510.0 39 475.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.50656762203769 19.602643622269095 13.617125068600028 23.273663687093187 2 14.688231471985846 21.154854821158768 40.753880960395804 23.40303274645958 3 19.102119418336198 26.328681002452512 27.974477570179644 26.594722009031642 4 12.209255029567286 17.683739557585806 37.0992772441413 33.00772816870561 5 13.377703722923695 39.94413582466089 32.89874735450149 13.779413097913926 6 30.799919334289022 37.65572312276352 17.563082618258903 13.981274924688552 7 27.79084019110033 34.4757560219115 20.056467145251258 17.676936641736912 8 24.694843906185717 38.422214331190766 19.135786053627378 17.74715570899614 9 25.35878954194783 19.70811827342947 19.17523386053323 35.757858324089476 10-11 23.884331826414876 29.72371250716262 26.30770060607672 20.08425506034579 12-13 24.293585267273365 26.74930024863548 26.810188973903827 22.14692551018733 14-15 21.869771490954896 27.875040147916252 24.69079266639211 25.564395694736735 16-17 21.987335004908427 30.77904882791739 25.386968592312837 21.846647574861343 18-19 21.883345529141728 29.563108617745836 27.123868760593155 21.429677092519277 20-21 23.249680607222032 28.41100527311214 26.879172459574896 21.460141660090933 22-23 22.764077566625577 28.50531252542065 26.538223276245066 22.192386631708704 24-25 22.35357868381503 28.43773995643056 26.94267944244877 22.26600191730564 26-27 22.21214124619619 28.789079411521072 26.71997861895317 22.278800723329567 28-29 22.17681312533015 28.759716938964964 26.771475724453087 22.2919942112518 30-31 22.489507298200593 28.39699022973371 26.766059831257195 22.347442640808502 32-33 22.169086304451618 28.43302959905043 26.855439828652294 22.54244426784566 34-35 22.48271669285764 28.713224865457065 26.76238158385188 22.04167685783342 36-37 22.538671434242154 28.497776138576413 26.625466104476164 22.338086322705266 38-39 22.296006357695383 28.59103124225898 26.71138516647144 22.401577233574198 40-41 22.59972166156015 28.356295082505234 26.690327778285695 22.353655477648925 42-43 22.25898459783229 28.323031359157298 26.781407997249335 22.636576045761085 44-45 22.391212274184298 28.49532778929963 26.863765869028068 22.24969406748801 46-47 22.513627465154716 28.575045815635537 26.416455335128187 22.49487138408156 48-49 22.239927601583638 28.459204774044107 26.908390682611405 22.39247694176085 50-51 22.371669189430804 28.631510509259726 26.797615968354616 22.199204332954853 52-53 22.284860926707246 28.499690149408156 26.761791710070497 22.4536572138141 54-55 22.35413452867139 28.373180540121503 26.82071023211252 22.45197469909459 56-57 22.470588603054377 28.632485311885063 26.65109416492264 22.245831920137917 58-59 22.367781454314127 28.46295598732715 26.69894794097466 22.47031461738406 60-61 22.39175808761877 28.552640729919364 26.84818274240195 22.20741844005992 62-63 22.306095032522315 28.46864955223694 26.839290612644902 22.385964802595844 64-65 22.017567033128504 28.566906510807055 26.81942596850815 22.596100487556296 66-67 22.179661928327803 28.647775836034928 26.59844688360693 22.57411535203034 68-69 22.277770221999198 28.44711554244498 26.858057143571777 22.41705709198405 70-71 22.11991543472569 28.348202597138695 26.911061872550285 22.620820095585334 72-73 22.154875313572102 28.419752893459727 26.79949359570494 22.62587819726323 74-75 22.061844713832816 28.726823470390073 26.59630582117999 22.615025994597122 76-77 22.155608606805583 28.477789455998938 26.798938801345646 22.567663135849838 78-79 22.181599921221565 28.572578540066807 26.861648501255925 22.384173037455707 80-81 22.12275405346898 28.64659814955341 26.715969186836645 22.514678610140958 82-83 22.259023009436042 28.636830415407772 26.73430439478509 22.369842180371098 84-85 22.423673592512678 28.472424650457995 26.72305394720081 22.380847809828516 86-87 22.147798405419792 28.587707773363586 26.799920904069612 22.464572917147013 88-89 22.192269905372736 28.46579091962818 26.905812772921923 22.436126402077157 90-91 22.283514722835537 28.596572813067862 26.71673928759843 22.40317317649817 92-93 22.162627756396027 28.457981472391484 26.734579345749687 22.644811425462798 94-95 22.08995329294142 28.657390907442494 26.78856146011142 22.464094339504665 96-97 22.0539835009164 28.55115223941257 26.897804941909314 22.49705931776172 98-99 21.96173146367772 28.639966233644422 26.613594084634745 22.784708218043114 100-101 21.815436579916238 28.72947261230522 26.82703812449641 22.62805268328213 102-103 21.80875592244817 28.529669687225955 26.911557586282896 22.750016804042975 104-105 21.647066547140717 28.754223332726326 26.860747839584086 22.73796228054887 106-107 21.572912514362088 28.86697774755551 26.850735886072485 22.70937385200991 108-109 21.611197730443244 28.79969051498775 26.695661348369924 22.89345040619908 110-111 21.43513051578764 29.11688784653053 26.70405923207435 22.743922405607478 112-113 21.473512262985945 29.184421552842604 26.842730526941054 22.499335657230393 114-115 21.26369817784832 29.34166692064415 26.551743809396545 22.84289109211099 116-117 21.28634067928928 29.146234823128935 26.876883799693825 22.690540697887958 118-119 21.179399202010217 29.295213233656252 26.761029023849815 22.764358540483716 120-121 21.238447680939114 29.24412175020809 26.854916549759476 22.662514019093322 122-123 21.276675557542305 29.274321676725883 26.730067232525446 22.718935533206366 124-125 21.33100654273094 29.308886922978335 26.579336473927544 22.780770060363178 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1845.0 1 1643.5 2 1279.0 3 904.0 4 568.0 5 399.5 6 319.0 7 285.5 8 301.0 9 299.0 10 262.5 11 245.0 12 242.5 13 247.5 14 270.5 15 293.0 16 323.5 17 351.0 18 413.0 19 517.0 20 665.0 21 886.0 22 1177.0 23 1564.5 24 2085.5 25 2727.0 26 3604.0 27 4645.0 28 5708.0 29 6786.5 30 7879.5 31 9197.5 32 10612.0 33 11786.5 34 12857.0 35 13971.5 36 15370.5 37 16819.5 38 17729.5 39 18381.0 40 19432.0 41 20561.0 42 21150.0 43 21823.5 44 22801.0 45 23684.0 46 24227.0 47 24360.0 48 24169.5 49 23971.5 50 23803.0 51 23209.0 52 22454.0 53 22048.5 54 21565.5 55 20033.0 56 17682.0 57 15650.0 58 14276.0 59 12644.0 60 10645.0 61 8796.5 62 6944.5 63 5088.0 64 3754.5 65 2889.5 66 2053.5 67 1481.5 68 1098.5 69 780.5 70 548.5 71 395.5 72 328.0 73 273.0 74 198.5 75 158.5 76 115.5 77 67.0 78 49.0 79 32.5 80 21.5 81 17.5 82 12.5 83 10.0 84 8.0 85 5.0 86 3.5 87 3.5 88 5.5 89 5.0 90 3.5 91 2.5 92 1.0 93 1.5 94 2.0 95 1.5 96 1.5 97 1.0 98 2.0 99 2.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05110141521228184 2 0.1889345901885283 3 0.02390983647547132 4 0.10689103365504825 5 0.021565734860229033 6 0.0354740711106666 7 0.03750562584387658 8 0.10017127569135369 9 0.11579861979296895 10-11 0.0466476221433215 12-13 0.03320810621593239 14-15 0.012892558883832575 16-17 0.029379406911036656 18-19 0.053132969945491826 20-21 0.18627794169125367 22-23 0.1031404710706606 24-25 0.07266715007251087 26-27 0.013283242486372956 28-29 0.006563484522678402 30-31 0.008516902535380306 32-33 0.005547707156073411 34-35 0.008204355653348002 36-37 0.004063109466419963 38-39 0.0077355353302995455 40-41 0.005547707156073411 42-43 0.009220133019952993 44-45 0.008907586137920687 46-47 0.017424488673300997 48-49 0.01734635195279292 50-51 0.00992336350452568 52-53 0.012423738560784117 54-55 0.013283242486372956 56-57 0.015783617542631394 58-59 0.01734635195279292 60-61 0.008204355653348002 62-63 0.01758076211431715 64-65 0.028598039705955893 66-67 0.024691203680552082 68-69 0.03391133670050507 70-71 0.025238160724108616 72-73 0.015471070660599091 74-75 0.009298269740461069 76-77 0.009610816622493374 78-79 0.021096914537180576 80-81 0.01758076211431715 82-83 0.019221633244986748 84-85 0.016018027704155623 86-87 0.0272697154573186 88-89 0.02867617642646397 90-91 0.026019527929189375 92-93 0.03281742261339201 94-95 0.026879031854778215 96-97 0.026800895134270142 98-99 0.03344251637745662 100-101 0.01851840276041406 102-103 0.02766039905985898 104-105 0.024144246636995548 106-107 0.031098414762214335 108-109 0.02054995749362404 110-111 0.018049582437365607 112-113 0.02719157873681052 114-115 0.013048832324848728 116-117 0.011486097914687203 118-119 0.026800895134270142 120-121 0.023675426313947093 122-123 0.017111941791268688 124-125 0.017190078511776768 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 639904.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.26045159316938 #Duplication Level Percentage of deduplicated Percentage of total 1 78.8852418437862 53.05856990449441 2 12.556027035681769 16.890480972719992 3 4.128946489159077 8.33144416394612 4 1.7790049665118064 4.78626709736301 5 0.8692628366718894 2.9233505473855357 6 0.5148165939519697 2.077607795812007 7 0.32485812648683193 1.5295073003850677 8 0.1987195633193042 1.0692774055403054 9 0.14788652849410405 0.8952223221953595 >10 0.5663400467996147 6.252560879205632 >50 0.02050685500686024 0.9778911413047179 >100 0.008156083182598643 0.8413678018959514 >500 0.0 0.0 >1k 2.3303094801433396E-4 0.3664526677519338 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2338 0.36536730509576437 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 7.813672050807621E-5 0.0 22-23 0.0 0.0 0.0 3.1254688203230486E-4 0.0 24-25 0.0 0.0 0.0 4.688203230484573E-4 0.0 26-27 0.0 0.0 0.0 0.0012501875281292194 0.0 28-29 0.0 0.0 0.0 0.002656648497274591 0.0 30-31 0.0 0.0 0.0 0.006094664199629944 0.0 32-33 0.0 0.0 0.0 0.012892558883832575 0.0 34-35 0.0 0.0 0.0 0.022190828624293644 0.0 36-37 0.0 0.0 0.0 0.03477084062609391 0.0 38-39 0.0 0.0 0.0 0.058915087263089465 0.0 40-41 0.0 0.0 0.0 0.08837263089463421 0.0 42-43 0.0 0.0 0.0 0.11517352602890434 0.0 44-45 0.0 0.0 0.0 0.1434590188528279 0.0 46-47 0.0 0.0 0.0 0.18041768765314797 0.0 48-49 0.0 0.0 0.0 0.22456493474021105 0.0 50-51 0.0 0.0 0.0 0.27433802570385557 0.0 52-53 0.0 0.0 0.0 0.3191885032754913 0.0 54-55 0.0 0.0 0.0 0.36614867230084513 0.0 56-57 0.0 0.0 0.0 0.41935977896684506 0.0 58-59 0.0 0.0 0.0 0.48538530779616945 0.0 60-61 0.0 0.0 0.0 0.5640689853478021 0.0 62-63 0.0 0.0 0.0 0.6417368855328299 0.0 64-65 0.0 0.0 0.0 0.7144040356053408 0.0 66-67 0.0 0.0 0.0 0.7993386507976197 0.0 68-69 0.0 0.0 0.0 0.9024791218682802 0.0 70-71 0.0 0.0 0.0 1.0303107966194929 0.0 72-73 0.0 0.0 0.0 1.1543137720658099 0.0 74-75 0.0 0.0 0.0 1.270346802020303 0.0 76-77 0.0 0.0 0.0 1.402554133119968 0.0 78-79 0.0 0.0 0.0 1.5720326799019855 0.0 80-81 0.0 0.0 0.0 1.7575292543881582 0.0 82-83 0.0 0.0 0.0 1.9513864579686953 0.0 84-85 0.0 0.0 0.0 2.1351640246036907 0.0 86-87 0.0 0.0 0.0 2.3238642046306945 0.0 88-89 0.0 0.0 0.0 2.5513201980297042 0.0 90-91 0.0 0.0 0.0 2.8022953443016454 0.0 92-93 0.0 0.0 0.0 3.0672569635445317 0.0 94-95 0.0 0.0 0.0 3.3358128719307896 0.0 96-97 0.0 0.0 0.0 3.5960081512226836 0.0 98-99 0.0 0.0 0.0 3.8858953843076462 0.0 100-101 0.0 0.0 0.0 4.235088388258239 0.0 102-103 0.0 0.0 0.0 4.620849377406611 0.0 104-105 0.0 0.0 0.0 5.004969495424314 0.0 106-107 0.0 0.0 0.0 5.363851452717908 0.0 108-109 0.0 0.0 0.0 5.755863379506926 0.0 110-111 0.0 0.0 0.0 6.208665674851227 0.0 112-113 0.0 0.0 0.0 6.695457443616543 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 850 0.0 56.127663 1 TAGATCG 45 3.1441395E-4 52.976234 5 GTATCAA 1410 0.0 46.524258 1 ATCAACG 1535 0.0 38.058655 3 TCAACGC 1585 0.0 36.86673 4 CAACGCA 1585 0.0 36.849403 5 AACGCAG 1635 0.0 34.628963 6 TATCAAC 1825 0.0 34.316296 2 GACCGTA 70 0.0027655975 34.056152 7 TACATGG 3285 0.0 32.863754 2 GTACATG 3460 0.0 32.747894 1 GAGCGTA 75 0.0038941284 31.75838 9 GTACCGT 85 0.0071501737 28.046244 6 TGACGCA 85 0.0071501737 28.046244 5 CGCAGAG 2040 0.0 27.754095 8 ACATGGG 3595 0.0 27.691935 3 ACGCAGA 2045 0.0 27.686237 7 GTATAGG 270 1.6643753E-9 24.296001 1 GTGTAGC 600 0.0 23.854258 1 GTAGCCC 450 0.0 23.844912 3 >>END_MODULE