FastQCFastQC Report
Fri 27 May 2016
SRR940034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639904
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50910.795588088213232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38360.5994649197379607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24940.3897459618942841No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11840.18502775416312447No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8000.12501875281292193No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19800.077.6829761
GTATCAA39600.056.6061971
CAACGTA350.00779672650.996629
ATCAACG49150.045.1081283
TATCAAC50300.044.317272
TCAACGC50100.044.2527854
CAACGCA50200.044.1162835
AACGCAG51550.043.1649366
GTGGTAT7400.041.087511
TGGTATC7850.038.723082
ACGCAGA60800.036.4021877
CGCAGAG61600.036.026028
GCAGAGT66950.033.05839
TATTCCG1102.030586E-532.4726945
GGACCGA1501.4774196E-731.7312286
TCCGTTG953.285979E-431.3137118
TCGCGCC400.005364167529.74802878-79
GTATAAC2052.3719622E-929.0815451
GAGTACT49150.028.80988912-13
ATAGCCC2900.028.7716923
Produced by FastQC (version 0.11.5)