##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940034_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 639904 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.08245924388658 34.0 33.0 34.0 31.0 34.0 2 33.11938040706106 34.0 33.0 34.0 31.0 34.0 3 33.17522159573936 34.0 33.0 34.0 31.0 34.0 4 36.4333321873281 37.0 37.0 37.0 35.0 37.0 5 36.44325086262939 37.0 37.0 37.0 35.0 37.0 6 36.525005313296994 37.0 37.0 37.0 35.0 37.0 7 36.50942797669651 37.0 37.0 37.0 35.0 37.0 8 36.50764958493774 37.0 37.0 37.0 35.0 37.0 9 38.36035561584237 39.0 39.0 39.0 37.0 39.0 10-11 38.34989545306796 39.0 39.0 39.0 37.0 39.0 12-13 38.33839294644197 39.0 39.0 39.0 37.0 39.0 14-15 39.904812284342654 41.0 40.0 41.0 38.0 41.0 16-17 39.91672891558734 41.0 40.0 41.0 38.0 41.0 18-19 39.9812214019603 41.0 40.0 41.0 38.0 41.0 20-21 39.9852673213482 41.0 40.0 41.0 38.0 41.0 22-23 39.94305864629695 41.0 40.0 41.0 38.0 41.0 24-25 39.915127894184124 41.0 40.0 41.0 38.0 41.0 26-27 39.81158111216682 41.0 40.0 41.0 37.0 41.0 28-29 39.72158636295444 41.0 40.0 41.0 37.0 41.0 30-31 39.52832299844977 41.0 39.5 41.0 36.5 41.0 32-33 39.307129663199476 41.0 39.0 41.0 35.0 41.0 34-35 39.17557086688003 41.0 39.0 41.0 35.0 41.0 36-37 39.05775553833075 41.0 39.0 41.0 35.0 41.0 38-39 38.92327130319548 41.0 39.0 41.0 35.0 41.0 40-41 38.77042728284243 41.0 39.0 41.0 35.0 41.0 42-43 38.57511829899485 41.0 39.0 41.0 35.0 41.0 44-45 38.29631554108116 41.0 38.0 41.0 35.0 41.0 46-47 37.904535680352055 41.0 38.0 41.0 33.5 41.0 48-49 37.54391752512877 41.0 38.0 41.0 33.0 41.0 50-51 37.5368023953593 41.0 38.0 41.0 33.0 41.0 52-53 37.43806024028604 41.0 38.0 41.0 33.0 41.0 54-55 37.29819941741261 41.0 37.5 41.0 33.0 41.0 56-57 37.129841351202685 40.5 37.0 41.0 33.0 41.0 58-59 36.941689690953645 40.0 37.0 41.0 32.5 41.0 60-61 36.72933205605841 40.0 36.0 41.0 32.0 41.0 62-63 36.48451095789369 39.5 35.5 41.0 32.0 41.0 64-65 36.19259842101315 39.0 35.0 41.0 31.0 41.0 66-67 35.88121812021803 39.0 35.0 41.0 31.0 41.0 68-69 35.53384570185528 37.5 35.0 41.0 31.0 41.0 70-71 35.17057714907236 37.0 35.0 40.0 31.0 41.0 72-73 34.80921903910587 36.5 35.0 39.0 30.0 41.0 74-75 34.445437909436414 36.0 35.0 39.0 30.0 41.0 76-77 34.079075142521376 36.0 35.0 38.0 30.0 40.5 78-79 33.72844911111667 35.0 35.0 37.0 29.0 39.0 80-81 33.40190794244137 35.0 35.0 37.0 29.0 39.0 82-83 33.10593542156323 35.0 35.0 36.0 29.0 38.5 84-85 32.84193019577937 35.0 35.0 36.0 29.0 37.0 86-87 32.625875131269694 35.0 34.0 36.0 29.0 37.0 88-89 32.50550082512377 35.0 34.5 35.0 28.5 36.5 90-91 32.47854756338451 35.0 35.0 35.0 29.0 36.0 92-93 32.38448032829925 35.0 35.0 35.0 29.0 36.0 94-95 32.296263189478424 35.0 35.0 35.0 29.0 36.0 96-97 32.22615345426814 35.0 35.0 35.0 29.0 36.0 98-99 32.15780413937091 35.0 35.0 35.0 29.0 36.0 100-101 32.096357422363354 35.0 35.0 35.0 28.5 35.0 102-103 31.62607828674301 35.0 33.5 35.0 26.0 35.0 104-105 31.99630725858879 35.0 34.0 35.0 27.0 35.0 106-107 32.034350465069764 35.0 34.5 35.0 29.0 35.0 108-109 32.020163962094315 35.0 35.0 35.0 29.0 35.0 110-111 31.977541943791568 35.0 35.0 35.0 27.0 35.0 112-113 31.943795475571335 35.0 35.0 35.0 27.0 35.0 114-115 31.879785092763914 35.0 34.0 35.0 27.0 35.0 116-117 31.81684752712907 35.0 34.0 35.0 27.0 35.0 118-119 31.755196873280994 35.0 34.0 35.0 25.0 35.0 120-121 31.673581505975896 35.0 34.0 35.0 25.0 35.0 122-123 31.56346967670151 35.0 34.0 35.0 25.0 35.0 124-125 30.540293544031606 34.5 32.0 35.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1554268016906093 1101 2 0.23887672143819572 1101 3 0.1449368962869073 1101 4 0.046651879745787994 1101 5 0.09159543974155326 1101 6 0.1324406423023703 1101 7 0.11914735960433376 1101 8 0.1668710971826144 1101 9 0.18722060166694376 1101 10-11 0.2642801326964559 1101 12-13 0.2528016682826788 1101 14-15 0.28032505369807126 1101 16-17 0.2998132069999926 1101 18-19 0.26526727881380907 1101 20-21 0.3290600892451394 1101 22-23 0.22745242091364304 1101 24-25 0.23610905221052292 1101 26-27 0.28963118945716104 1101 28-29 0.29248600969103933 1101 30-31 0.2614885842433452 1101 32-33 0.29310297244178685 1101 34-35 0.42428009438485503 1101 36-37 0.4035091084511251 1101 38-39 0.39851774769581993 1101 40-41 0.41843661860740156 1101 42-43 0.4415574286479824 1101 44-45 0.3864811041323222 1101 46-47 0.30809383093189524 1101 48-49 0.13159167857459408 1101 50-51 0.07929168856490065 1101 52-53 0.03415339980892895 1101 54-55 0.12046051927239887 1101 56-57 0.10673707597116788 1101 58-59 0.004580195609285909 1101 60-61 0.025850673534193902 1101 62-63 -0.00734423535242712 1101 64-65 -0.15879020500290153 1101 66-67 -0.24741553588577148 1101 68-69 -0.11856316358149854 1101 70-71 -0.07768044328594925 1101 72-73 -0.06313958002285602 1101 74-75 -0.15095284050210012 1101 76-77 -0.04925301039981633 1101 78-79 0.011432513469578964 1101 80-81 0.14024440509587066 1101 82-83 0.07031137800856158 1101 84-85 0.03243507211831087 1101 86-87 0.10257162784749596 1101 88-89 0.026903927063166577 1101 90-91 0.008403862359891434 1101 92-93 -0.08060818271182058 1101 94-95 -0.033248281570323 1101 96-97 -0.09822767491953499 1101 98-99 -0.07636943942863894 1101 100-101 0.024849514714134102 1101 102-103 0.01918419559216744 1101 104-105 0.08981897850841136 1101 106-107 0.04221290881368844 1101 108-109 -0.018636552583025434 1101 110-111 0.07191783309293953 1101 112-113 0.006845539698012715 1101 114-115 0.048621981911715295 1101 116-117 0.09189116256595398 1101 118-119 0.1223750977035678 1101 120-121 0.1422111245880906 1101 122-123 0.276302836014068 1101 124-125 0.25902165814574474 1102 1 0.0792785391044788 1102 2 0.11125588718527268 1102 3 0.05350503184687483 1102 4 -0.017051251767981057 1102 5 0.008347934898374376 1102 6 0.03243819005979276 1102 7 0.0419875469556672 1102 8 0.04693191279849884 1102 9 0.0513050569012492 1102 10-11 0.17155164499445164 1102 12-13 0.13699144733562463 1102 14-15 0.19708061415810363 1102 16-17 0.23375592261341893 1102 18-19 0.18412563728262654 1102 20-21 0.25743314890819846 1102 22-23 0.18672373703223144 1102 24-25 0.18887886019992806 1102 26-27 0.17875610669397446 1102 28-29 0.12896434412787983 1102 30-31 0.12282039718628113 1102 32-33 0.1794445943550329 1102 34-35 0.2724556259084352 1102 36-37 0.2545997433367333 1102 38-39 0.40841254648931624 1102 40-41 0.4186174714973703 1102 42-43 0.4642008235326074 1102 44-45 0.551057234003089 1102 46-47 0.4467466914728533 1102 48-49 0.5670762417075821 1102 50-51 0.5089460449738397 1102 52-53 0.4543696876041139 1102 54-55 0.4396321517302795 1102 56-57 0.4885113960869063 1102 58-59 0.41533695766819534 1102 60-61 0.5743958806260849 1102 62-63 0.5615453892092361 1102 64-65 0.5725022494466856 1102 66-67 0.4715727914395629 1102 68-69 0.3974469151354896 1102 70-71 0.4495517105186977 1102 72-73 0.4090305320446248 1102 74-75 0.4397218940426484 1102 76-77 0.2877770786165925 1102 78-79 0.3685403385756416 1102 80-81 0.38478203475819583 1102 82-83 0.22831243247286181 1102 84-85 0.28910823722780776 1102 86-87 0.45213574172963433 1102 88-89 0.4636544604259285 1102 90-91 0.3951531696013646 1102 92-93 0.49958628012444706 1102 94-95 0.42263893979361455 1102 96-97 0.4347182040868134 1102 98-99 0.5866188633742766 1102 100-101 0.5763312822905817 1102 102-103 0.46095876093217214 1102 104-105 0.491784450932947 1102 106-107 0.4451286252363573 1102 108-109 0.372830256313712 1102 110-111 0.40440514273760897 1102 112-113 0.5437487251611195 1102 114-115 0.3848060472394579 1102 116-117 0.4007970945407422 1102 118-119 0.42683634000965753 1102 120-121 0.40076332057131836 1102 122-123 0.5470150898702251 1102 124-125 0.39236847881324266 1103 1 0.2301650921649454 1103 2 0.2225528028300161 1103 3 0.23697373705471136 1103 4 0.1561716605853647 1103 5 0.11357965969531847 1103 6 0.1669075853987536 1103 7 0.11418588002356245 1103 8 0.18986868617005115 1103 9 0.16955637080474162 1103 10-11 0.22937003433457903 1103 12-13 0.3005392521877681 1103 14-15 0.3602634458192 1103 16-17 0.3187216814922209 1103 18-19 0.30582760681068066 1103 20-21 0.3495722899603848 1103 22-23 0.3698540125538088 1103 24-25 0.3130228321699917 1103 26-27 0.35237717161938775 1103 28-29 0.4022761772223191 1103 30-31 0.33971401081902286 1103 32-33 0.3372457304170382 1103 34-35 0.5135038289131586 1103 36-37 0.5142018561997119 1103 38-39 0.5215940509751675 1103 40-41 0.5546131183625818 1103 42-43 0.5693263743103358 1103 44-45 0.6126514369016363 1103 46-47 0.6719351503569726 1103 48-49 0.4433901451953943 1103 50-51 0.6071570127500223 1103 52-53 0.5069590064977803 1103 54-55 0.437966546803807 1103 56-57 0.5819777123793131 1103 58-59 0.7013233143067197 1103 60-61 0.5863072784070482 1103 62-63 0.5314915058944791 1103 64-65 0.6601754458155398 1103 66-67 0.6298583464642533 1103 68-69 0.6267496172642808 1103 70-71 0.733461037906288 1103 72-73 0.5438250853174438 1103 74-75 0.44751904857892555 1103 76-77 0.49564590061584823 1103 78-79 0.34571956032008444 1103 80-81 0.3665201034527854 1103 82-83 0.47180927379579174 1103 84-85 0.4553833871986086 1103 86-87 0.3870377961969069 1103 88-89 0.32536280039513343 1103 90-91 0.35721586535970573 1103 92-93 0.2533488219304658 1103 94-95 0.4249084094543889 1103 96-97 0.31610855286846373 1103 98-99 0.38717096827420505 1103 100-101 0.35479255531706855 1103 102-103 0.22989755161009384 1103 104-105 0.2156324628244377 1103 106-107 0.2664510226457182 1103 108-109 0.2572996516526658 1103 110-111 0.2461328594653267 1103 112-113 0.37997530051812944 1103 114-115 0.33595936252321223 1103 116-117 0.2897390704392464 1103 118-119 0.20975028596708256 1103 120-121 0.1360913982906986 1103 122-123 0.26733281562272637 1103 124-125 0.21292111458327057 1104 1 0.08509865879935319 1104 2 0.14956815445332694 1104 3 0.17021045074981345 1104 4 -0.06823334065919795 1104 5 -0.07182694549415913 1104 6 0.0341631016289341 1104 7 0.06060027517137456 1104 8 0.07037847259057628 1104 9 0.1436723232077881 1104 10-11 0.1841701598222869 1104 12-13 0.18886845414785824 1104 14-15 0.13058192241029332 1104 16-17 0.15875432007732826 1104 18-19 0.10489042577700047 1104 20-21 0.033942737901398345 1104 22-23 0.14370219894723135 1104 24-25 0.14408943344164982 1104 26-27 0.16003265780322096 1104 28-29 0.1749578582272875 1104 30-31 0.12139486849505943 1104 32-33 0.05396019140169983 1104 34-35 0.06498766206164674 1104 36-37 0.10158886940096323 1104 38-39 0.16672970877668547 1104 40-41 0.22475868114987918 1104 42-43 0.21683481011792338 1104 44-45 0.1682069924571934 1104 46-47 0.17186003447967835 1104 48-49 0.26293456003534743 1104 50-51 0.3273437419837961 1104 52-53 0.26571258370732664 1104 54-55 0.11304913744731948 1104 56-57 0.1874057918725356 1104 58-59 0.14349366238780448 1104 60-61 0.1964632395883399 1104 62-63 0.07235012455768697 1104 64-65 -0.052655818885448014 1104 66-67 0.0947954332348715 1104 68-69 0.054356322313687144 1104 70-71 0.10623020681538264 1104 72-73 0.18561587728917317 1104 74-75 0.06472043719165299 1104 76-77 0.04669082138332925 1104 78-79 -0.10623647185308016 1104 80-81 -0.062482873197957645 1104 82-83 0.06386746356206174 1104 84-85 0.10861241256273502 1104 86-87 0.11549389980379487 1104 88-89 0.04692223546481955 1104 90-91 -3.6074915297490406E-4 1104 92-93 0.08599138376482074 1104 94-95 0.03819198956546899 1104 96-97 0.08471703860602275 1104 98-99 -0.019218447162252517 1104 100-101 0.08275672357073915 1104 102-103 0.1713513531362736 1104 104-105 0.11002174864918857 1104 106-107 -0.05000112191257955 1104 108-109 -0.12460714279935914 1104 110-111 -0.09785661842041193 1104 112-113 0.014815664550287266 1104 114-115 0.10763213419598117 1104 116-117 0.07000450932952873 1104 118-119 0.0659321017387633 1104 120-121 -0.011880252093497745 1104 122-123 -0.030915511576143473 1104 124-125 0.08000651544430681 1105 1 0.14191164907278875 1105 2 0.15527957426646566 1105 3 0.11532000249775365 1105 4 -0.01928490196797128 1105 5 0.06189060800626578 1105 6 0.059761445994546136 1105 7 0.0645318303695177 1105 8 0.11614909632142911 1105 9 0.1876875824843367 1105 10-11 0.14185838230680758 1105 12-13 0.16274335632736125 1105 14-15 0.25723982666654877 1105 16-17 0.2706890689434758 1105 18-19 0.17973625491288203 1105 20-21 0.13740691650468762 1105 22-23 0.19450904688626736 1105 24-25 0.20539748260915758 1105 26-27 0.29500980780041175 1105 28-29 0.32090891843570546 1105 30-31 0.32158279913942067 1105 32-33 0.4270680944329541 1105 34-35 0.34304286812962204 1105 36-37 0.3434717499857314 1105 38-39 0.42490519622179335 1105 40-41 0.5669084693676183 1105 42-43 0.6486717601073337 1105 44-45 0.6389357857666269 1105 46-47 0.8864965745958102 1105 48-49 0.8225110243162703 1105 50-51 0.9474946917973739 1105 52-53 0.8754322298097179 1105 54-55 0.7680241833130594 1105 56-57 0.7805676529047361 1105 58-59 0.8499572371341912 1105 60-61 0.802884011112809 1105 62-63 0.8454332391910526 1105 64-65 0.6724970549113465 1105 66-67 0.7581488134643948 1105 68-69 0.8696106988350394 1105 70-71 0.8210908513425537 1105 72-73 0.7723558138481721 1105 74-75 0.7433230056732043 1105 76-77 0.7600057940080021 1105 78-79 0.7188273366536606 1105 80-81 0.697155417959074 1105 82-83 0.6680688087650068 1105 84-85 0.7422989805658133 1105 86-87 0.7511981280991762 1105 88-89 0.7363675662386129 1105 90-91 0.74290783169684 1105 92-93 0.7357493365890448 1105 94-95 0.7202988016189664 1105 96-97 0.7174437767843358 1105 98-99 0.6611085002762564 1105 100-101 0.5917915181224842 1105 102-103 0.6583770768638146 1105 104-105 0.6831446400140351 1105 106-107 0.747801213673327 1105 108-109 0.6949563796319822 1105 110-111 0.7767169706946007 1105 112-113 0.693247452499957 1105 114-115 0.7115747865257305 1105 116-117 0.6862887573115088 1105 118-119 0.49588470113375394 1105 120-121 0.6023957033046834 1105 122-123 0.6378869926285198 1105 124-125 0.6043964254134195 1106 1 0.15504956163918848 1106 2 0.1705796735679428 1106 3 0.14712488858517503 1106 4 0.052402527089718376 1106 5 0.06536971136646486 1106 6 0.07118892963253387 1106 7 0.07241486203376013 1106 8 0.07152593762516801 1106 9 0.07798999029662212 1106 10-11 0.14181292240172638 1106 12-13 0.11924888183676075 1106 14-15 0.14933221824112053 1106 16-17 0.1882240908281645 1106 18-19 0.24783803395784787 1106 20-21 0.1895899153525633 1106 22-23 0.22044179179795265 1106 24-25 0.2261016281263153 1106 26-27 0.21052470515336807 1106 28-29 0.22607742551821275 1106 30-31 0.15568255805986553 1106 32-33 0.1386058183407357 1106 34-35 0.11147792510366372 1106 36-37 0.20703947174838788 1106 38-39 0.17878598046198846 1106 40-41 0.06342581445233009 1106 42-43 -0.07130872054592885 1106 44-45 0.03503220054280831 1106 46-47 -0.01098636728512048 1106 48-49 0.047771957068285076 1106 50-51 0.01652271640823244 1106 52-53 0.08200630033223177 1106 54-55 0.11016111138839335 1106 56-57 0.10442685789623596 1106 58-59 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-0.9967474817068371 2216 102-103 -0.991093822607084 2216 104-105 -0.8796647131178972 2216 106-107 -0.9847896721433678 2216 108-109 -0.9436338234743502 2216 110-111 -0.9168314164021751 2216 112-113 -0.9613437746719136 2216 114-115 -0.8816151776319785 2216 116-117 -1.1065939871841373 2216 118-119 -0.959013745700176 2216 120-121 -0.9353667134899943 2216 122-123 -0.9595272418824905 2216 124-125 -1.2392629021168737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 9.0 5 39.0 6 117.0 7 206.0 8 355.0 9 643.0 10 1256.0 11 1864.0 12 2536.0 13 4014.0 14 7492.0 15 7348.0 16 3018.0 17 2251.0 18 2090.0 19 1842.0 20 1590.0 21 1422.0 22 1475.0 23 1569.0 24 1775.0 25 2177.0 26 2703.0 27 3213.0 28 3771.0 29 4743.0 30 5763.0 31 7247.0 32 9015.0 33 12158.0 34 17360.0 35 31804.0 36 71442.0 37 238049.0 38 185806.0 39 1736.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.7068532460976 18.850138871739404 14.311838561798314 22.131169320364677 2 15.50980477955655 19.956805210879335 41.6399954992405 22.893394510323617 3 19.31027046286703 25.646074483163854 29.619645477517686 25.42400957645143 4 12.235728973337382 16.77297495234906 38.16237938599923 32.82891668831433 5 12.915349523639785 38.00500905193843 34.06264754346962 15.016993880952157 6 30.555234293680588 37.39680981748505 17.31110062234503 14.736855266489329 7 27.148447267090063 34.04401285192779 20.7807733660049 18.026766514977247 8 25.845751862779416 35.433752562884436 19.415099764964744 19.305395809371404 9 26.64415287293094 16.33166850027504 20.334300145021754 36.68987848177226 10-11 24.750431314697206 27.38879581937291 26.747293344001598 21.113479521928287 12-13 26.1213400760114 24.598220983147474 27.909420788118215 21.37101815272291 14-15 22.98657298594789 25.25245974396159 27.339179001850276 24.421788268240235 16-17 21.228574911236684 28.822057683652545 27.701186427964196 22.24818097714657 18-19 20.86375456318448 26.290271665749863 32.00230034505176 20.843673426013904 20-21 22.029554433164975 25.22183014952243 32.23116279941991 20.517452617892683 22-23 21.506897909686455 25.427017177576637 31.985032129819473 21.081052782917435 24-25 21.16661249187378 25.533986347952194 32.22639645946892 21.073004700705106 26-27 21.03299869980497 25.87567822673401 31.99612754413162 21.0951955293294 28-29 21.006041531229684 25.82402985447817 32.10848814822223 21.06144046606991 30-31 21.25140743877788 25.5601039844289 32.043581571283795 21.144907005509424 32-33 21.11232396759824 25.525098706289306 32.05334866897899 21.30922865713346 34-35 21.33414283292038 25.81780164603458 31.83958781156162 21.008467709483412 36-37 21.47956161831789 25.73648341378874 31.62264669893218 21.161308268961196 38-39 21.32731047491348 25.74396913245991 31.62586703610199 21.302853356524622 40-41 21.70231784767715 25.61454530679602 31.399631819772967 21.28350502575386 42-43 21.46620013470876 25.575520511830774 31.306190508189573 21.652088845270896 44-45 21.70017057339383 25.839485982586357 30.96325131797834 21.49709212604147 46-47 21.94666852111295 26.235440187465763 30.02140166446972 21.796489626951566 48-49 22.002176924753886 26.3292250976139 29.72189903476125 21.946698942870963 50-51 22.091879351798386 26.662483678924698 29.32206687519095 21.92357009408597 52-53 22.168481522228333 26.67548569785468 29.129916362454367 22.026116417462617 54-55 22.079405660849126 26.629697579636947 29.20617780167025 22.084718957843677 56-57 22.126425330385977 26.914863442546476 28.95113342725492 22.007577799812626 58-59 22.159043849107437 26.604823573720225 29.218751709254114 22.017380867918217 60-61 22.210814189717144 26.606266604156897 29.378496640100018 21.80442256602594 62-63 22.181295944391657 26.46623556033405 29.56318447767165 21.78928401760264 64-65 22.021278424496444 26.47387001345529 29.490839932051564 22.014011629996702 66-67 22.152879898609314 26.58223693977662 29.20865883941422 22.056224322199842 68-69 22.287284624045846 26.437083381192483 29.395912007920007 21.879719986841664 70-71 22.20269652561543 26.465332338382318 29.22155501467032 22.110416121331934 72-73 22.194266639996 26.59984935240286 29.110929139370906 22.094954868230236 74-75 22.02566480727172 26.82302878480896 28.909749673192913 22.24155673472641 76-77 22.007227833017524 26.675157742571937 28.972827254790882 22.34478716961966 78-79 22.01576651370018 26.69204040315643 29.09928965518858 22.192903427954818 80-81 21.988063834575186 26.876609616442465 28.82768352752913 22.30764302145322 82-83 22.176529604440667 26.676501475221283 28.829715082262343 22.31725383807571 84-85 22.368876508530597 26.561155801079074 28.633893444704466 22.436074245685866 86-87 22.059263623343206 26.624873906768755 28.736598565252418 22.57926390463562 88-89 21.960950392558885 26.66228059208881 28.795803745561834 22.580965269790468 90-91 22.090596472749407 26.802244400913576 28.60079683889836 22.50636228743865 92-93 22.021123563548453 26.566745038103523 28.738954354693654 22.673177043654373 94-95 21.92216572016165 26.670891298300663 28.770991626790522 22.635951354747164 96-97 21.915699591499948 26.634937849858257 28.80480136020828 22.64456119843351 98-99 21.83311871780767 26.63274491173676 28.570223033455015 22.963913337000548 100-101 21.830557263712418 26.601667282644964 28.751678964093806 22.816096489548816 102-103 21.97212394359154 26.238232609891483 28.77642583887583 23.013217607641145 104-105 21.90062884432665 26.351687128069212 28.67984885232785 23.06783517527629 106-107 21.788084903559525 26.420665648282355 28.635846867296188 23.155402580861928 108-109 21.8501597511492 26.33811610068112 28.53853288357661 23.273191264593066 110-111 21.629494424163624 26.482487998199726 28.571863904585687 23.31615367305096 112-113 21.751092156864278 26.56197379761167 28.566025971103453 23.120908074420594 114-115 21.516990367124453 26.6802835478487 28.361582892113045 23.441143192913806 116-117 21.480259071122664 26.409064520086297 28.668552894468913 23.442123514322127 118-119 21.337374570833394 26.70506311112778 28.522369796672308 23.435192521366517 120-121 21.41519886607814 26.603407078885393 28.54315419680795 23.438239858228513 122-123 21.373490492692245 26.618664562179394 28.371822269798912 23.636022675329443 124-125 21.294203230351016 26.632697790824967 28.291708307320135 23.781390671503885 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 159.0 1 130.5 2 305.0 3 457.5 4 256.0 5 160.5 6 466.0 7 1269.0 8 2257.5 9 2485.5 10 1702.5 11 933.0 12 765.5 13 817.5 14 872.0 15 920.5 16 990.5 17 1045.0 18 1106.0 19 1171.0 20 1299.5 21 1524.0 22 1761.0 23 2088.5 24 2548.0 25 3212.0 26 4182.5 27 5137.5 28 6019.5 29 6998.5 30 8119.0 31 9449.5 32 10694.5 33 11761.5 34 12789.0 35 13744.0 36 15168.0 37 16597.0 38 17311.5 39 18034.0 40 18952.0 41 19813.0 42 20576.5 43 21360.5 44 22243.5 45 23357.0 46 24142.0 47 24397.5 48 24167.5 49 23765.5 50 23573.0 51 22785.5 52 21961.5 53 21545.0 54 20819.0 55 19392.0 56 17148.5 57 14982.5 58 13595.0 59 12063.0 60 10098.0 61 8259.5 62 6550.5 63 4904.5 64 3631.5 65 2674.5 66 1834.0 67 1363.5 68 1001.0 69 624.0 70 422.0 71 318.0 72 252.5 73 227.0 74 168.5 75 112.0 76 75.0 77 50.5 78 36.0 79 13.0 80 5.5 81 5.0 82 3.5 83 1.0 84 1.0 85 0.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016408711306696005 2 0.0018752812921938292 3 1.5627344101615243E-4 4 0.13783317497624645 5 0.04094364154623193 6 0.010314047107066059 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 7.813672050807621E-5 32-33 7.813672050807621E-5 34-35 3.9068360254038105E-4 36-37 7.813672050807621E-4 38-39 0.0013283242486372955 40-41 0.0 42-43 7.813672050807621E-4 44-45 5.469570435565335E-4 46-47 7.813672050807621E-5 48-49 0.0016408711306696005 50-51 0.0016408711306696005 52-53 0.0 54-55 0.0 56-57 0.0013283242486372955 58-59 7.813672050807621E-4 60-61 6.250937640646097E-4 62-63 0.0 64-65 0.001093914087113067 66-67 4.688203230484573E-4 68-69 8.595039255888384E-4 70-71 0.0010157773666049908 72-73 0.0 74-75 7.813672050807621E-5 76-77 0.0025785117767665147 78-79 5.469570435565335E-4 80-81 0.0 82-83 0.0 84-85 2.3441016152422864E-4 86-87 7.813672050807621E-5 88-89 0.0 90-91 7.813672050807621E-5 92-93 3.9068360254038105E-4 94-95 9.376406460969146E-4 96-97 3.1254688203230486E-4 98-99 0.0 100-101 7.813672050807621E-5 102-103 0.0 104-105 0.0 106-107 2.3441016152422864E-4 108-109 3.9068360254038105E-4 110-111 0.0 112-113 7.813672050807621E-5 114-115 0.0018752812921938292 116-117 3.9068360254038105E-4 118-119 1.5627344101615243E-4 120-121 1.5627344101615243E-4 122-123 2.3441016152422864E-4 124-125 7.032304845726859E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 639904.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.473924373102484 #Duplication Level Percentage of deduplicated Percentage of total 1 76.32515625214802 47.68332039461926 2 13.927564728934936 17.402192511539415 3 4.652839435569531 8.720434170538791 4 1.9990111982370482 4.995442976785853 5 1.0029480205699115 3.1329049393618744 6 0.588392561456495 2.2055515431677475 7 0.3613369856867013 1.5801897661897057 8 0.23461555165681364 1.1725883384769196 9 0.17310502080568263 0.9733094980576694 >10 0.6909632662880807 7.21974872547089 >50 0.02754191221038391 1.1670697046578966 >100 0.015022787669543685 1.5551483536944706 >500 5.007595889567495E-4 0.22039871557816057 >1k 7.511393834351243E-4 1.1753555350278908 >5k 2.5037979447837475E-4 0.7963448268335098 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5091 0.795588088213232 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3836 0.5994649197379607 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2494 0.3897459618942841 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1184 0.18502775416312447 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 800 0.12501875281292193 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 7.813672050807621E-5 0.0 22-23 0.0 0.0 0.0 3.1254688203230486E-4 0.0 24-25 0.0 0.0 0.0 4.688203230484573E-4 0.0 26-27 0.0 0.0 0.0 0.0012501875281292194 0.0 28-29 0.0 0.0 0.0 0.002656648497274591 0.0 30-31 0.0 0.0 0.0 0.006094664199629944 0.0 32-33 0.0 0.0 0.0 0.012892558883832575 0.0 34-35 0.0 0.0 0.0 0.02226896534480172 0.0 36-37 0.0 0.0 0.0 0.03492711406711006 0.0 38-39 0.0 0.0 0.0 0.059305770865629846 0.0 40-41 0.0 0.0 0.0 0.08899772465869882 0.0 42-43 0.0 0.0 0.0 0.11587675651347701 0.0 44-45 0.0 0.0 0.0 0.14408411261689252 0.0 46-47 0.0 0.0 0.0 0.18112091813772066 0.0 48-49 0.0 0.0 0.0 0.2246430714607191 0.0 50-51 0.0 0.0 0.0 0.2746505725858879 0.0 52-53 0.0 0.0 0.0 0.319891733760064 0.0 54-55 0.0 0.0 0.0 0.36739885982897436 0.0 56-57 0.0 0.0 0.0 0.42068810321548233 0.0 58-59 0.0 0.0 0.0 0.4875731359703956 0.0 60-61 0.0 0.0 0.0 0.5667256338450768 0.0 62-63 0.0 0.0 0.0 0.644862354353153 0.0 64-65 0.0 0.0 0.0 0.7184671450717608 0.0 66-67 0.0 0.0 0.0 0.8044175376306446 0.0 68-69 0.0 0.0 0.0 0.9084175126268941 0.0 70-71 0.0 0.0 0.0 1.0367180077011553 0.0 72-73 0.0 0.0 0.0 1.1618148972345852 0.0 74-75 0.0 0.0 0.0 1.2791762514377156 0.0 76-77 0.0 0.0 0.0 1.4128681802270342 0.0 78-79 0.0 0.0 0.0 1.5846908286242938 0.0 80-81 0.0 0.0 0.0 1.771593864079612 0.0 82-83 0.0 0.0 0.0 1.9672482122318349 0.0 84-85 0.0 0.0 0.0 2.151963419512927 0.0 86-87 0.0 0.0 0.0 2.3424607441116168 0.0 88-89 0.0 0.0 0.0 2.5724171125668853 0.0 90-91 0.0 0.0 0.0 2.8258144971745764 0.0 92-93 0.0 0.0 0.0 3.095229909486423 0.0 94-95 0.0 0.0 0.0 3.367614517177577 0.0 96-97 0.0 0.0 0.0 3.6336700505075763 0.0 98-99 0.0 0.0 0.0 3.929417537630645 0.0 100-101 0.0 0.0 0.0 4.284001975296294 0.0 102-103 0.0 0.0 0.0 4.673982347352103 0.0 104-105 0.0 0.0 0.0 5.064197129569436 0.0 106-107 0.0 0.0 0.0 5.430970895634346 0.0 108-109 0.0 0.0 0.0 5.8311090413562034 0.0 110-111 0.0 0.0 0.0 6.292350102515377 0.0 112-113 0.0 0.0 0.0 6.78531467220083 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1980 0.0 77.682976 1 GTATCAA 3960 0.0 56.606197 1 CAACGTA 35 0.007796726 50.99662 9 ATCAACG 4915 0.0 45.108128 3 TATCAAC 5030 0.0 44.31727 2 TCAACGC 5010 0.0 44.252785 4 CAACGCA 5020 0.0 44.116283 5 AACGCAG 5155 0.0 43.164936 6 GTGGTAT 740 0.0 41.08751 1 TGGTATC 785 0.0 38.72308 2 ACGCAGA 6080 0.0 36.402187 7 CGCAGAG 6160 0.0 36.02602 8 GCAGAGT 6695 0.0 33.0583 9 TATTCCG 110 2.030586E-5 32.472694 5 GGACCGA 150 1.4774196E-7 31.731228 6 TCCGTTG 95 3.285979E-4 31.313711 8 TCGCGCC 40 0.0053641675 29.748028 78-79 GTATAAC 205 2.3719622E-9 29.081545 1 GAGTACT 4915 0.0 28.809889 12-13 ATAGCCC 290 0.0 28.771692 3 >>END_MODULE