FastQCFastQC Report
Fri 27 May 2016
SRR940033_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940033_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794354
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23580.2968449834708455No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12850.056.1613961
GTATCAA24450.050.2509231
GTTCGAC255.273161E-447.7094616-17
ATCAACG27700.043.240263
TCAACGC27800.042.4443474
CAACGCA28450.041.456315
AACGCAG28700.041.0951926
GTACATG61350.037.6228871
TATCAAC33350.036.6571662
TACATGG61250.035.829652
GACGCAT350.002794534433.9870172-73
CGCAGAG35250.033.628058
ACGCAGA35850.033.231397
ACATGGG62850.032.805213
CATGGGG15600.031.3248254
TACCGTC800.00533168129.7836347
CATGGGA44100.029.729224
CGCAAAA1653.338646E-728.9139162
GCAGAGT41000.028.4706769
ATGGGAT19100.028.3802195
Produced by FastQC (version 0.11.5)