##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940033_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 794354 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.790801078612308 33.0 31.0 34.0 26.0 34.0 2 30.870876208843917 34.0 31.0 34.0 26.0 34.0 3 31.037689745378007 34.0 31.0 34.0 26.0 34.0 4 34.077508264577254 37.0 35.0 37.0 30.0 37.0 5 34.38666891587378 37.0 35.0 37.0 31.0 37.0 6 34.361858818612355 37.0 35.0 37.0 31.0 37.0 7 34.455030125107946 37.0 36.0 37.0 31.0 37.0 8 34.40178686076988 37.0 36.0 37.0 31.0 37.0 9 35.88427703517575 39.0 38.0 39.0 30.0 39.0 10-11 35.97387059170093 39.0 38.0 39.0 31.0 39.0 12-13 35.90225516583287 39.0 38.0 39.0 30.5 39.0 14-15 37.33755919904728 41.0 39.0 41.0 31.0 41.0 16-17 37.41469672211633 41.0 39.0 41.0 31.0 41.0 18-19 37.459801927100514 41.0 39.0 41.0 31.0 41.0 20-21 37.50237110910249 41.0 39.0 41.0 30.5 41.0 22-23 37.5991654854133 41.0 39.0 41.0 30.0 41.0 24-25 37.74801285069377 41.0 39.0 41.0 30.0 41.0 26-27 37.837701453004584 41.0 39.0 41.0 30.0 41.0 28-29 37.814432859908806 41.0 39.0 41.0 30.0 41.0 30-31 37.768123531825864 41.0 39.0 41.0 30.0 41.0 32-33 37.72162159440249 41.0 38.5 41.0 30.0 41.0 34-35 37.63531372662566 41.0 38.0 41.0 30.0 41.0 36-37 37.49455973029657 41.0 38.0 41.0 30.0 41.0 38-39 37.38963547738162 41.0 38.0 41.0 30.0 41.0 40-41 37.294494645963894 41.0 38.0 41.0 30.0 41.0 42-43 37.13592239731908 40.0 38.0 41.0 30.0 41.0 44-45 37.00598788449482 40.0 38.0 41.0 30.0 41.0 46-47 36.86214395596925 40.0 37.0 41.0 30.0 41.0 48-49 36.72862791652085 40.0 37.0 41.0 29.0 41.0 50-51 36.57540529788986 40.0 37.0 41.0 29.0 41.0 52-53 36.71443651067408 40.0 37.0 41.0 30.0 41.0 54-55 36.531312865548614 40.0 36.0 41.0 30.0 41.0 56-57 36.426717810950784 40.0 36.0 41.0 30.0 41.0 58-59 36.241004010806265 40.0 35.5 41.0 30.0 41.0 60-61 36.01045755418869 39.5 35.0 41.0 29.5 41.0 62-63 35.72675658459578 39.0 35.0 41.0 29.0 41.0 64-65 35.42775953793901 39.0 35.0 41.0 29.0 41.0 66-67 35.108890998219934 38.5 35.0 41.0 28.0 41.0 68-69 34.75913320761273 37.0 35.0 40.0 28.0 41.0 70-71 34.39709499794802 37.0 35.0 39.5 28.0 41.0 72-73 34.02230680527825 36.0 34.5 39.0 27.5 41.0 74-75 33.627097616427946 36.0 34.0 39.0 27.0 41.0 76-77 33.25595578293809 35.5 34.0 37.5 26.5 40.0 78-79 32.88398434451139 35.0 34.0 37.0 26.0 39.0 80-81 32.52799066914751 35.0 34.0 37.0 26.0 39.0 82-83 32.18556965786035 35.0 34.0 36.0 25.0 38.0 84-85 31.901426190338313 35.0 33.0 36.0 25.0 37.0 86-87 31.667845192445686 35.0 33.0 35.5 24.0 37.0 88-89 31.46498538435005 35.0 33.0 35.0 23.5 36.5 90-91 31.288096994538957 35.0 33.0 35.0 23.0 36.0 92-93 31.126108133149703 35.0 33.0 35.0 20.5 36.0 94-95 30.985863985074666 35.0 33.0 35.0 20.0 36.0 96-97 30.869960370313485 35.0 33.0 35.0 19.5 35.5 98-99 30.787359917618595 35.0 33.0 35.0 19.0 35.0 100-101 30.68416726044056 35.0 33.0 35.0 18.0 35.0 102-103 30.000669097153157 34.0 31.0 35.0 18.5 35.0 104-105 30.86425636429098 35.0 33.0 35.0 19.0 35.0 106-107 30.960979613623145 35.0 33.0 35.0 19.0 35.0 108-109 30.942310984775048 35.0 33.0 35.0 19.0 35.0 110-111 30.889481264020827 35.0 33.0 35.0 18.0 35.0 112-113 30.827081502705344 35.0 33.0 35.0 17.5 35.0 114-115 30.762389942015776 35.0 33.0 35.0 16.0 35.0 116-117 30.679838585819418 35.0 33.0 35.0 8.5 35.0 118-119 30.588975444197423 35.0 33.0 35.0 2.0 35.0 120-121 30.485742502712895 35.0 33.0 35.0 2.0 35.0 122-123 30.349341729254213 35.0 33.0 35.0 2.0 35.0 124-125 29.122851398746654 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21395966131240485 1101 2 0.22248825479789858 1101 3 0.3721797759275667 1101 4 0.22064181009706374 1101 5 0.24907708084517566 1101 6 0.38538117321085963 1101 7 0.3867717484932811 1101 8 0.15965642673926794 1101 9 0.28386553340470755 1101 10-11 0.19798511025715015 1101 12-13 0.34656333239684045 1101 14-15 0.4044056397900917 1101 16-17 0.4032680083609037 1101 18-19 0.36624329559290203 1101 20-21 0.12990094106321237 1101 22-23 0.18383841730101835 1101 24-25 0.17115688270929041 1101 26-27 0.2746274081828872 1101 28-29 0.1939937479036118 1101 30-31 0.23988336867388682 1101 32-33 0.29074078462447517 1101 34-35 0.2846106272614577 1101 36-37 0.3100347326684627 1101 38-39 0.27756403912858474 1101 40-41 0.24036954863187532 1101 42-43 0.3082520669527682 1101 44-45 0.27834296533310265 1101 46-47 0.3811033043086667 1101 48-49 0.26163371082859754 1101 50-51 0.2945094788006699 1101 52-53 0.26832075079700246 1101 54-55 0.3346222821513223 1101 56-57 0.21246421954369765 1101 58-59 0.27463999129486893 1101 60-61 0.21319523492268644 1101 62-63 0.240927923712853 1101 64-65 0.2162313179095534 1101 66-67 0.20696060528624827 1101 68-69 0.23317731756131366 1101 70-71 0.21072374580899123 1101 72-73 0.2522108295478205 1101 74-75 0.2831787045741976 1101 76-77 0.26797757197167726 1101 78-79 0.3257925841011371 1101 80-81 0.30663717447002625 1101 82-83 0.37234237962904615 1101 84-85 0.21036391535680465 1101 86-87 0.3417661546014408 1101 88-89 0.31301891071255383 1101 90-91 0.1858986174939723 1101 92-93 0.2646938877374687 1101 94-95 0.21364455857926856 1101 96-97 0.1763293363912659 1101 98-99 0.054624912113837354 1101 100-101 0.15206884547949784 1101 102-103 0.174653651280817 1101 104-105 0.18359800679376193 1101 106-107 0.2138670591851337 1101 108-109 0.15667330756987496 1101 110-111 0.11879674507088467 1101 112-113 0.2761605015384667 1101 114-115 0.19238104736054495 1101 116-117 0.22808258215870936 1101 118-119 0.07399033700608015 1101 120-121 0.15533912151447282 1101 122-123 0.009891831936055695 1101 124-125 -0.12216126301566277 1102 1 0.19049581551353256 1102 2 0.2774515655987706 1102 3 0.3622698888603182 1102 4 0.36646045845377984 1102 5 0.2967309498188442 1102 6 0.4299589304789748 1102 7 0.4396356358749358 1102 8 0.5606878825930579 1102 9 0.521135877274034 1102 10-11 0.538595261527206 1102 12-13 0.5479969241857674 1102 14-15 0.7302229696683327 1102 16-17 0.6711929767934848 1102 18-19 0.6360202191769346 1102 20-21 0.5344344785916704 1102 22-23 0.5420548201874666 1102 24-25 0.6331497122148164 1102 26-27 0.6274076228920364 1102 28-29 0.5919587270868618 1102 30-31 0.6503845426268384 1102 32-33 0.5849475930444825 1102 34-35 0.5331788103474011 1102 36-37 0.5166428453679472 1102 38-39 0.6570540922227011 1102 40-41 0.7122119194590226 1102 42-43 0.7609780212015664 1102 44-45 0.7460311147471401 1102 46-47 0.7548564547115717 1102 48-49 0.7418900027600159 1102 50-51 0.8151827141947265 1102 52-53 0.6709146036446612 1102 54-55 0.6564375227482913 1102 56-57 0.6138871620833868 1102 58-59 0.589639205278857 1102 60-61 0.5644906400251983 1102 62-63 0.6744583526747547 1102 64-65 0.6948171990335084 1102 66-67 0.6449231503553889 1102 68-69 0.6382732368704325 1102 70-71 0.6859708337464383 1102 72-73 0.6413800359759136 1102 74-75 0.6532634421716281 1102 76-77 0.6411992741273949 1102 78-79 0.6596460402896156 1102 80-81 0.521570971637324 1102 82-83 0.5978382036020236 1102 84-85 0.6377319787654763 1102 86-87 0.7277918040788585 1102 88-89 0.7991155551848337 1102 90-91 0.6889245255960255 1102 92-93 0.576470436062241 1102 94-95 0.6137404018223194 1102 96-97 0.7126929727549012 1102 98-99 0.6167764661435626 1102 100-101 0.6626907109237017 1102 102-103 0.6763428250512078 1102 104-105 0.5760000210231873 1102 106-107 0.6492200310863296 1102 108-109 0.5778561856055404 1102 110-111 0.5263658257364128 1102 112-113 0.6376431812952568 1102 114-115 0.6288146645048229 1102 116-117 0.6422126145628511 1102 118-119 0.5753098296961348 1102 120-121 0.5681450972646118 1102 122-123 0.6779826243923317 1102 124-125 0.5302441995420821 1103 1 -0.035060065730970535 1103 2 0.18246272210693348 1103 3 0.2341036439276074 1103 4 0.3061205987127238 1103 5 0.3868593842586563 1103 6 0.17564936778617835 1103 7 0.23511161697965832 1103 8 0.21438149437049958 1103 9 0.28515229997309177 1103 10-11 0.3658782579718718 1103 12-13 0.30946848655553083 1103 14-15 0.40474455586657143 1103 16-17 0.3097849237686674 1103 18-19 0.27759521105132023 1103 20-21 0.30429884576142285 1103 22-23 0.2933549172959431 1103 24-25 0.2220383684687306 1103 26-27 0.3349605490078318 1103 28-29 0.312548516538925 1103 30-31 0.29611456111134515 1103 32-33 0.3256096256226826 1103 34-35 0.35330977718496115 1103 36-37 0.42925568163347094 1103 38-39 0.4808002063879755 1103 40-41 0.4606690511496794 1103 42-43 0.4491600986051978 1103 44-45 0.3709795196356609 1103 46-47 0.2945986486453762 1103 48-49 0.44146288496763475 1103 50-51 0.2618433389990571 1103 52-53 0.2358506655867103 1103 54-55 0.3283429626177963 1103 56-57 0.3423303755565428 1103 58-59 0.24743450379272502 1103 60-61 0.32334954559069473 1103 62-63 0.3406589185759188 1103 64-65 0.30989373925974917 1103 66-67 0.34420540502686947 1103 68-69 0.31644935217229886 1103 70-71 0.3824290661328149 1103 72-73 0.35073206119620437 1103 74-75 0.3610062981180775 1103 76-77 0.3353499338469135 1103 78-79 0.3272075207253522 1103 80-81 0.20908405735400493 1103 82-83 0.3285401363997664 1103 84-85 0.3561986418429193 1103 86-87 0.33212908356584236 1103 88-89 0.3037815478747774 1103 90-91 0.27322346019435884 1103 92-93 0.2683429348209678 1103 94-95 0.10560632033494599 1103 96-97 0.18062075355693352 1103 98-99 0.13528440531018404 1103 100-101 0.17026696700295219 1103 102-103 0.11772030984186443 1103 104-105 0.30554193619384407 1103 106-107 0.3965580224362988 1103 108-109 0.470000966971881 1103 110-111 0.5004461366430704 1103 112-113 0.4664580789724333 1103 114-115 0.3424428526839094 1103 116-117 0.3249669859399802 1103 118-119 0.4628035734772986 1103 120-121 0.4861911230950682 1103 122-123 0.4633225339837388 1103 124-125 0.29450439044898147 1104 1 -0.11919449417677797 1104 2 0.10662239476960167 1104 3 -0.15820963161920076 1104 4 0.09972719172051825 1104 5 -0.2896826365947405 1104 6 0.1312576317776717 1104 7 0.11396131020060807 1104 8 0.04919937110454953 1104 9 0.18447254061069884 1104 10-11 0.09953432246626193 1104 12-13 0.25828663447546774 1104 14-15 0.24820498765035381 1104 16-17 0.15620796180846952 1104 18-19 0.07058565374236991 1104 20-21 0.008460452617640613 1104 22-23 0.11797889611154488 1104 24-25 0.14264022170532087 1104 26-27 0.11991511539952171 1104 28-29 0.19885183397997253 1104 30-31 0.21332160556389823 1104 32-33 0.26826427636115824 1104 34-35 0.2254865026550945 1104 36-37 0.28837111709621865 1104 38-39 0.20200913717774682 1104 40-41 0.29163249887960063 1104 42-43 0.2040349642585113 1104 44-45 0.14263451135053629 1104 46-47 0.207903407758522 1104 48-49 0.25237951324952235 1104 50-51 0.2087891078011168 1104 52-53 0.15942609215614567 1104 54-55 0.17641754272831633 1104 56-57 0.12837267656890106 1104 58-59 0.12460424024389027 1104 60-61 0.1204346959692586 1104 62-63 0.18944336765977 1104 64-65 0.019492523708834142 1104 66-67 -0.04688504145280348 1104 68-69 -0.03954894095174666 1104 70-71 -0.16467851690291724 1104 72-73 -0.18229628770041728 1104 74-75 -0.21586742695924244 1104 76-77 -0.14751201458390284 1104 78-79 -0.13855575791218655 1104 80-81 -0.21669076662441 1104 82-83 -0.19237158660776288 1104 84-85 -0.14448580345230866 1104 86-87 -0.1768036005165463 1104 88-89 -0.13994538387610334 1104 90-91 -0.23914740247590416 1104 92-93 -0.3516014920096886 1104 94-95 -0.2344114463295277 1104 96-97 -0.193400933339003 1104 98-99 -0.20839835903126414 1104 100-101 -0.21643016819717786 1104 102-103 -0.2662146175062361 1104 104-105 -0.32459937957620966 1104 106-107 -0.28284790098160073 1104 108-109 -0.2807851730358166 1104 110-111 -0.3033045039339157 1104 112-113 -0.22549368184160556 1104 114-115 -0.25380271811255994 1104 116-117 -0.26238279003255016 1104 118-119 -0.22688797250166814 1104 120-121 -0.3294573003377863 1104 122-123 -0.41542396901426315 1104 124-125 -0.3254001561022726 1105 1 0.17585462496412063 1105 2 0.1760682452861957 1105 3 0.23414753320450288 1105 4 0.11624604571547081 1105 5 -0.06666187150896974 1105 6 0.11655722701991067 1105 7 0.03333734525542553 1105 8 0.20795839410831007 1105 9 0.21951111580223426 1105 10-11 0.06458982146057224 1105 12-13 0.11802823131075968 1105 14-15 0.34177012232447623 1105 16-17 0.24443474458592362 1105 18-19 0.18202058592299863 1105 20-21 0.014056195307077246 1105 22-23 0.07228558368782956 1105 24-25 -0.04133450337839406 1105 26-27 0.05433060430831915 1105 28-29 0.11480156302721412 1105 30-31 0.16708294938261048 1105 32-33 0.16329467617168092 1105 34-35 0.15861408253220333 1105 36-37 0.22191319643179241 1105 38-39 0.2758038892707688 1105 40-41 0.16721389644952467 1105 42-43 0.23481154905039148 1105 44-45 0.27177067155744083 1105 46-47 0.14632445134209604 1105 48-49 0.3123883380767083 1105 50-51 0.11052456163982072 1105 52-53 0.20529991240855594 1105 54-55 0.2904875071806856 1105 56-57 0.21714193790030833 1105 58-59 0.07911340221003371 1105 60-61 -0.0010465952061124995 1105 62-63 -0.04878876349778949 1105 64-65 -0.049636721806649575 1105 66-67 0.04180351664302151 1105 68-69 0.0311614194335732 1105 70-71 0.08874492390102517 1105 72-73 0.10140184972621569 1105 74-75 0.11275232804674573 1105 76-77 0.07308574329875483 1105 78-79 -0.01401824953146047 1105 80-81 0.10040943718706785 1105 82-83 0.05037180766527172 1105 84-85 0.08769549517258568 1105 86-87 0.09735961676330973 1105 88-89 0.17855112917107618 1105 90-91 0.10817783442868034 1105 92-93 0.040171171273492234 1105 94-95 0.07428941304054959 1105 96-97 0.043346544804268916 1105 98-99 0.1612534996263335 1105 100-101 0.25792339411049525 1105 102-103 0.1459516101475664 1105 104-105 0.2595994044313663 1105 106-107 0.24479367350509662 1105 108-109 0.22537835750630109 1105 110-111 0.16047788958078968 1105 112-113 0.09057218345943951 1105 114-115 0.06571237482509673 1105 116-117 0.019740917999378382 1105 118-119 -0.007297333694967989 1105 120-121 0.11804500615113511 1105 122-123 0.07614373670258345 1105 124-125 -0.09787051557071891 1106 1 -0.10642114585209939 1106 2 0.020683618716756058 1106 3 0.01383695297491272 1106 4 -0.11118926824537567 1106 5 -0.09799979472178677 1106 6 0.184020458304758 1106 7 0.1520059651654151 1106 8 0.1386830813106883 1106 9 0.24905149777520563 1106 10-11 0.15201321239762677 1106 12-13 0.24639975709429507 1106 14-15 0.23393218820645245 1106 16-17 0.2675101577114276 1106 18-19 0.26507462484730837 1106 20-21 0.24318817736437381 1106 22-23 0.21927683771366446 1106 24-25 0.1582334687624467 1106 26-27 0.19002133997342696 1106 28-29 0.2194106477897364 1106 30-31 0.30826990821181965 1106 32-33 0.21071868507032576 1106 34-35 0.10478780971305923 1106 36-37 0.15337398495079668 1106 38-39 0.1723334896313773 1106 40-41 0.050288291755926195 1106 42-43 0.09413440854046229 1106 44-45 0.09024536057735588 1106 46-47 0.0660785026256292 1106 48-49 0.07228299379939074 1106 50-51 0.15572572631567994 1106 52-53 0.055765019388609005 1106 54-55 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2216 70-71 -0.9607982211130377 2216 72-73 -0.842501044276375 2216 74-75 -0.7871456430917121 2216 76-77 -0.7005654453886692 2216 78-79 -0.7765881597756419 2216 80-81 -0.7884135114584048 2216 82-83 -0.9080696303843325 2216 84-85 -0.7861552583281863 2216 86-87 -0.7265515919659045 2216 88-89 -0.6968863608051876 2216 90-91 -0.7855418171035176 2216 92-93 -0.8832286988302336 2216 94-95 -0.8492207892768846 2216 96-97 -0.9340811335670942 2216 98-99 -0.9239317845587394 2216 100-101 -0.983556854717083 2216 102-103 -1.0155292529005848 2216 104-105 -0.9580991338374183 2216 106-107 -1.0008610003153429 2216 108-109 -0.8877784177447055 2216 110-111 -0.8681251681002102 2216 112-113 -0.8880446941844049 2216 114-115 -0.9165736176472912 2216 116-117 -0.9110185088645828 2216 118-119 -0.8240136309003816 2216 120-121 -0.9795407088982913 2216 122-123 -0.9334386729302189 2216 124-125 -1.13345379193699 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14565.0 3 2817.0 4 2382.0 5 1729.0 6 2186.0 7 2402.0 8 2305.0 9 2396.0 10 2851.0 11 3560.0 12 3767.0 13 3301.0 14 3220.0 15 3244.0 16 3241.0 17 3073.0 18 2580.0 19 2178.0 20 1853.0 21 1658.0 22 1696.0 23 2884.0 24 5490.0 25 9060.0 26 9601.0 27 6044.0 28 5718.0 29 6640.0 30 7603.0 31 9255.0 32 12132.0 33 16942.0 34 25762.0 35 45980.0 36 107597.0 37 286142.0 38 169957.0 39 543.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.99953525453316 20.874628298086986 13.597142508088963 22.528693939290893 2 14.745238638901048 21.834957984465415 40.79638392648764 22.623419450145896 3 18.601181107319594 28.49490663208129 28.06514977901457 24.83876248158455 4 11.600852927906924 18.69384072125842 37.88648295782494 31.818823393009723 5 12.89222253560852 41.450556036827365 32.44391784354413 13.213303584019986 6 29.057100397964835 39.097904387688274 18.33207395093446 13.512921263412423 7 27.6284040420323 35.11817806300815 20.636705723525797 16.616712171433754 8 25.162813908239496 37.84226205478209 19.558209752704325 17.43671428427409 9 26.24350094846764 19.543853236448886 19.832237816444728 34.380407998638745 10-11 23.593811694604973 30.417018532547672 26.41383961716992 19.57533015567743 12-13 24.075341538527407 28.056247996922266 26.64513679486154 21.223273669688783 14-15 22.180869547700226 28.5437115676133 24.869877570342915 24.40554131434356 16-17 21.501960058379897 31.535595239444458 25.597178724835 21.36526597734064 18-19 21.556213461202987 30.088729093809498 27.569248557971164 20.785808887016355 20-21 23.18311909293757 28.93626521661804 27.142561626435892 20.738054064008494 22-23 22.763192800426975 28.90881628851727 26.77766554525159 21.55032536580417 24-25 21.93896223580465 29.116874298035476 27.37256922637919 21.57159423978069 26-27 21.92595241272855 29.334991929452585 27.17262661694236 21.566429040876503 28-29 21.764876212538006 28.969539408665433 27.51827068947067 21.74731368932589 30-31 22.09180526004356 28.911480693449498 27.424807060393558 21.571906986113383 32-33 21.707165995419825 29.073654180510218 27.22572149068417 21.99345833338579 34-35 22.14783605498575 28.98838379737082 27.406570869033974 21.457209278609454 36-37 22.29589758858302 28.64940128448602 27.078886426282423 21.975814700648538 38-39 21.94107993470812 29.13118115222664 27.152217827603508 21.77552108546173 40-41 22.09095475678799 28.8684979485262 27.38437405184645 21.656173242839355 42-43 21.81145060080779 28.92262522284782 27.303390308512032 21.962533867832356 44-45 21.79697744382675 29.0303634256476 27.32796440467025 21.8446947258554 46-47 21.8789622457395 29.058252488306064 27.144098260546578 21.918687005407854 48-49 21.65899023678995 28.957348597215333 27.185138364605656 22.19852280138906 50-51 21.86553371288712 28.865581555801207 27.296900534581614 21.971984196730062 52-53 21.999891720114125 29.043938970434557 27.078659041748192 21.877510267703133 54-55 21.968384473011596 28.956536519647962 27.206539667350764 21.868539339989677 56-57 21.8433375261421 29.197561875406848 26.962089705961972 21.99701089248908 58-59 21.80959797833917 28.697611491333713 27.3250956782215 22.167694852105612 60-61 21.92117482348438 29.185384204748043 27.1291409405437 21.764300031223875 62-63 21.684719480985525 29.054359904180004 27.413395282659543 21.84752533217493 64-65 21.559062029155598 28.877239354076252 27.23063371524527 22.333064901522885 66-67 21.661715837072165 29.226789129917407 26.766338052466622 22.345156980543806 68-69 21.709969153156873 28.859019411662107 27.297157922110305 22.13385351307071 70-71 21.51996363379941 28.794148164564614 27.554700406979087 22.13118779465689 72-73 21.631757307609256 29.027219668015363 27.039869759582785 22.301153264792593 74-75 21.31279118737406 29.398711646361846 26.993545641624163 22.294951524639934 76-77 21.483325726696147 29.218488849719076 27.224500716073084 22.073684707511685 78-79 21.60189025039018 28.949149469169793 27.15396562872042 22.294994651719602 80-81 21.35824472756599 29.34935277760115 27.11755140225749 22.174851092575373 82-83 21.832094325748187 29.201608191552587 26.904974162158897 22.06132332054033 84-85 21.712960427286344 29.023641389713074 26.95450828263904 22.30888990036155 86-87 21.548768763210035 29.01797603824512 27.240519782078863 22.19273541646598 88-89 21.793499544784286 28.84203951750912 27.143574910373502 22.22088602733309 90-91 21.7900601463332 29.072469626196174 27.05999459797405 22.077475629496572 92-93 21.796269944212874 28.86001586721908 27.124507297661477 22.219206890906573 94-95 21.417876993671126 28.763010962830137 27.42080091873201 22.398311124766728 96-97 21.76442181466513 28.799556247013264 27.26297968041978 22.17304225790183 98-99 21.371007095628215 28.73524787474318 27.12986907498673 22.76387595464188 100-101 21.275477247859136 29.065436564847914 27.189434756714082 22.469651430578867 102-103 21.5935763827676 28.939647313041156 27.23457813058786 22.232198173603383 104-105 21.218886443980647 28.9804732628822 27.395039459559854 22.4056008335773 106-107 20.964929899466007 29.29998948507731 27.316069131524053 22.419011483932632 108-109 21.215694520692832 29.265614690728142 26.850317557051984 22.668373231527045 110-111 20.95522177579284 29.593693300075675 26.930772107383127 22.520312816748365 112-113 20.902758189950436 29.495217492041746 27.315134282951202 22.286890035056615 114-115 20.721281629333234 29.86475103841539 26.95372551587117 22.460241816380208 116-117 20.72232119811897 29.735975675012117 27.147794446298057 22.393908680570856 118-119 20.596416514406098 29.667578761004 27.190631913899637 22.545372810690267 120-121 20.600601129983342 29.69492716304171 27.172170728973988 22.53230097800096 122-123 20.746517285144446 29.68718522100004 27.199549740118456 22.366747753737055 124-125 20.52594600651971 29.707620305537475 27.235594750486964 22.53083893745585 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1847.0 1 1576.5 2 1219.5 3 962.0 4 656.0 5 453.5 6 369.0 7 345.5 8 356.0 9 349.5 10 293.5 11 259.5 12 265.0 13 301.0 14 315.5 15 338.0 16 404.5 17 445.5 18 513.5 19 630.5 20 779.0 21 1014.0 22 1308.5 23 1731.5 24 2252.0 25 2951.5 26 4098.0 27 5355.5 28 6188.0 29 7532.0 30 9620.0 31 11676.5 32 14508.0 33 17822.5 34 21100.5 35 23539.0 36 26349.5 37 29006.5 38 29610.5 39 30370.0 40 29979.5 41 29115.5 42 28513.5 43 28031.5 44 28289.5 45 29164.0 46 29259.5 47 28065.5 48 27301.5 49 26452.0 50 25622.5 51 23883.5 52 22142.0 53 21281.5 54 20578.0 55 19445.0 56 17572.5 57 16114.0 58 15315.0 59 13759.5 60 12329.0 61 10900.0 62 8799.0 63 6263.0 64 4225.0 65 3457.5 66 2594.0 67 1889.5 68 1405.0 69 975.5 70 749.5 71 597.5 72 514.5 73 527.0 74 426.5 75 327.0 76 243.5 77 134.5 78 86.5 79 55.0 80 36.0 81 22.0 82 17.5 83 14.5 84 14.0 85 12.5 86 7.0 87 6.5 88 7.0 89 5.0 90 3.5 91 4.0 92 3.0 93 3.0 94 3.5 95 2.0 96 2.5 97 4.0 98 3.5 99 1.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046704617840408684 2 0.21035961296852534 3 0.023163476233518054 4 0.10725696603781185 5 0.020393930162119156 6 0.03952897574632972 7 0.03751476042167598 8 0.10196965081059578 9 0.12198591559934237 10-11 0.04689345052709497 12-13 0.03317160862789135 14-15 0.012840622694667618 16-17 0.03084267215876045 18-19 0.0529360965010562 20-21 0.195504774949204 22-23 0.10857879484461588 24-25 0.07547013044561995 26-27 0.01359595344141277 28-29 0.0061685344317520905 30-31 0.008056861298614975 32-33 0.007679195925242398 34-35 0.009504578563209853 36-37 0.0035878210470394814 38-39 0.009378690105418995 40-41 0.005916757516170372 42-43 0.011329961201177309 44-45 0.011644682345654456 46-47 0.0174984956329294 48-49 0.01863149175304713 50-51 0.01107818428559559 52-53 0.014603061103739642 54-55 0.015610168766066513 56-57 0.017246718717347682 58-59 0.019575655186478573 60-61 0.011329961201177309 62-63 0.01970154364426943 64-65 0.029646731809747293 66-67 0.026562464593871245 68-69 0.03392693937463649 70-71 0.026436576136080386 72-73 0.0164284437417071 74-75 0.010637574683327584 76-77 0.011518793887863598 78-79 0.021841647426714034 80-81 0.019197989813105994 82-83 0.021401037824446027 84-85 0.016680220657288816 86-87 0.026877185738348394 88-89 0.028324903002943273 90-91 0.026121854991603237 92-93 0.03323455285678677 94-95 0.02719190688282554 96-97 0.027506628027302686 98-99 0.03443049320579993 100-101 0.02077159553549173 102-103 0.027947237629570694 104-105 0.0234781973779952 106-107 0.030779727929865025 108-109 0.02089748399328259 110-111 0.019009157126419707 112-113 0.026436576136080386 114-115 0.014477172645948784 116-117 0.013721841899203628 118-119 0.026562464593871245 120-121 0.023037587775727196 122-123 0.01837971483746541 124-125 0.01850560329525627 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 794354.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.12103726767465 #Duplication Level Percentage of deduplicated Percentage of total 1 72.0016580422259 34.64794469984319 2 13.36567147416006 12.863399502311045 3 5.531512415490133 7.985463452772174 4 2.844327989653756 5.474880527664742 5 1.6408595850073087 3.947993262057894 6 1.0059933769336826 2.9045666869475766 7 0.6674062042100998 2.2481395177830046 8 0.5023030718643737 1.933707587268239 9 0.3674502629927507 1.5913879019541908 >10 1.8697633033896137 16.238998681078026 >50 0.13458285924614097 4.433142526197102 >100 0.06768438706971067 5.275778308164292 >500 5.246851709658963E-4 0.15691877284823377 >1k 2.6234258548294814E-4 0.297678573110326 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2358 0.2968449834708455 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 1.2588845779085898E-4 16-17 0.0 0.0 0.0 0.0 1.2588845779085898E-4 18-19 0.0 0.0 0.0 1.2588845779085898E-4 1.2588845779085898E-4 20-21 0.0 0.0 0.0 3.147211444771475E-4 1.2588845779085898E-4 22-23 0.0 0.0 0.0 6.923865178497244E-4 1.2588845779085898E-4 24-25 0.0 0.0 0.0 0.0013847730356994488 1.2588845779085898E-4 26-27 0.0 0.0 0.0 0.0016994941801765965 1.2588845779085898E-4 28-29 0.0 0.0 0.0 0.0027066018425034684 1.2588845779085898E-4 30-31 0.0 0.0 0.0 0.0087492478164647 1.2588845779085898E-4 32-33 0.0 0.0 0.0 0.019449766728687715 1.2588845779085898E-4 34-35 0.0 0.0 0.0 0.0349969912658588 1.2588845779085898E-4 36-37 0.0 0.0 0.0 0.0595452405350763 1.2588845779085898E-4 38-39 0.0 0.0 0.0 0.10014426817262834 1.2588845779085898E-4 40-41 0.0 0.0 0.0 0.14647122063966445 1.2588845779085898E-4 42-43 0.0 0.0 0.0 0.18832913285512504 1.2588845779085898E-4 44-45 0.0 0.0 0.0 0.2414540620428675 1.2588845779085898E-4 46-47 1.2588845779085898E-4 0.0 0.0 0.3015658006380027 1.2588845779085898E-4 48-49 1.2588845779085898E-4 0.0 0.0 0.3711191735674523 1.2588845779085898E-4 50-51 1.2588845779085898E-4 0.0 0.0 0.44973651545784377 1.2588845779085898E-4 52-53 1.2588845779085898E-4 0.0 0.0 0.5235700959521825 1.2588845779085898E-4 54-55 1.2588845779085898E-4 0.0 0.0 0.6013691628669334 1.2588845779085898E-4 56-57 1.2588845779085898E-4 0.0 0.0 0.6970443907879862 1.2588845779085898E-4 58-59 1.2588845779085898E-4 0.0 0.0 0.800776480007654 1.2588845779085898E-4 60-61 1.2588845779085898E-4 0.0 0.0 0.9161532515729762 1.2588845779085898E-4 62-63 1.2588845779085898E-4 0.0 0.0 1.0351807884142334 1.2588845779085898E-4 64-65 1.2588845779085898E-4 0.0 0.0 1.149739285003915 1.2588845779085898E-4 66-67 1.2588845779085898E-4 0.0 0.0 1.289097807778396 1.2588845779085898E-4 68-69 1.2588845779085898E-4 0.0 0.0 1.448409651112728 1.2588845779085898E-4 70-71 1.2588845779085898E-4 0.0 0.0 1.6242128824176625 1.2588845779085898E-4 72-73 1.2588845779085898E-4 0.0 0.0 1.803226269396264 1.2588845779085898E-4 74-75 1.2588845779085898E-4 0.0 0.0 1.9794701103034669 1.2588845779085898E-4 76-77 1.2588845779085898E-4 0.0 0.0 2.1692469604231865 1.2588845779085898E-4 78-79 1.2588845779085898E-4 0.0 0.0 2.403210659227498 1.2588845779085898E-4 80-81 1.2588845779085898E-4 0.0 0.0 2.6568129574471833 1.2588845779085898E-4 82-83 1.2588845779085898E-4 0.0 0.0 2.920801053434615 1.2588845779085898E-4 84-85 1.2588845779085898E-4 0.0 0.0 3.184726205193151 1.2588845779085898E-4 86-87 1.2588845779085898E-4 0.0 0.0 3.454756947154543 1.2588845779085898E-4 88-89 1.2588845779085898E-4 0.0 0.0 3.7726253030764623 1.2588845779085898E-4 90-91 1.2588845779085898E-4 0.0 0.0 4.113153581400736 1.2588845779085898E-4 92-93 1.2588845779085898E-4 0.0 0.0 4.48232148387243 1.2588845779085898E-4 94-95 1.2588845779085898E-4 0.0 0.0 4.859986857245007 1.2588845779085898E-4 96-97 1.2588845779085898E-4 0.0 0.0 5.233057301908217 1.2588845779085898E-4 98-99 1.2588845779085898E-4 0.0 0.0 5.641880068584031 1.2588845779085898E-4 100-101 1.2588845779085898E-4 1.2588845779085898E-4 0.0 6.128501902174597 1.2588845779085898E-4 102-103 1.2588845779085898E-4 1.2588845779085898E-4 0.0 6.6430709733947335 1.2588845779085898E-4 104-105 1.2588845779085898E-4 1.2588845779085898E-4 0.0 7.165130407853425 1.2588845779085898E-4 106-107 1.2588845779085898E-4 1.2588845779085898E-4 0.0 7.680203032904725 1.2588845779085898E-4 108-109 1.2588845779085898E-4 1.2588845779085898E-4 0.0 8.21806146881617 1.2588845779085898E-4 110-111 1.2588845779085898E-4 1.2588845779085898E-4 0.0 8.813073264564665 1.2588845779085898E-4 112-113 1.2588845779085898E-4 1.2588845779085898E-4 0.0 9.451201857106529 1.2588845779085898E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1285 0.0 56.161396 1 GTATCAA 2445 0.0 50.250923 1 GTTCGAC 25 5.273161E-4 47.70946 16-17 ATCAACG 2770 0.0 43.24026 3 TCAACGC 2780 0.0 42.444347 4 CAACGCA 2845 0.0 41.45631 5 AACGCAG 2870 0.0 41.095192 6 GTACATG 6135 0.0 37.622887 1 TATCAAC 3335 0.0 36.657166 2 TACATGG 6125 0.0 35.82965 2 GACGCAT 35 0.0027945344 33.98701 72-73 CGCAGAG 3525 0.0 33.62805 8 ACGCAGA 3585 0.0 33.23139 7 ACATGGG 6285 0.0 32.80521 3 CATGGGG 1560 0.0 31.324825 4 TACCGTC 80 0.005331681 29.783634 7 CATGGGA 4410 0.0 29.72922 4 CGCAAAA 165 3.338646E-7 28.913916 2 GCAGAGT 4100 0.0 28.470676 9 ATGGGAT 1910 0.0 28.380219 5 >>END_MODULE