FastQCFastQC Report
Fri 27 May 2016
SRR940033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940033_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794354
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66140.8326262598287414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48470.6101813549122935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31580.3975557497035327No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13670.17208952180010423No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9060.11405494275851824No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26900.077.952711
GTATCAA54200.058.252881
TGGTATC10300.046.263542
ATCAACG68500.045.3036583
TCAACGC69350.044.8342744
CAACGCA71200.043.714435
AACGCAG72750.042.9385536
TATCAAC73150.042.6680762
TATAACG853.4537752E-642.045392
ATGGGCG758.1768E-539.6745385
GTGGTAT12300.038.2615741
ACGCAGA83850.036.9682127
CGCAGAG84100.036.787578
GCAGAGT90600.033.9512759
GTACATG57700.032.4186331
TACATGG58550.031.3335742
ACATGGG58900.030.3383563
TGCACCG1403.088042E-629.7559035
TAACGCA1454.0307714E-628.7551824
GAGTACT59000.028.63914712-13
Produced by FastQC (version 0.11.5)