FastQCFastQC Report
Fri 27 May 2016
SRR940032_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940032_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277522
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20750.050.6128771
GGTATCA14650.045.2118031
TCAACGC24800.040.863674
ATCAACG25200.040.211883
CAACGCA25950.038.7986685
AACGCAG28150.035.7664456
GTACATG64200.035.3195421
TATCAAC29100.035.2641752
TACATGG64500.034.7797432
TATTCCG2750.034.662095
ACATGGG66650.032.2859153
ACGCAGA33850.029.039747
CGCAGAG34500.028.4926158
ATTCCGT3350.028.4539576
CATGGGA33900.026.729084
TCCGTTG2256.4646883E-926.4779858
CATGGGG39050.026.1044864
CCGTTGC2752.0936568E-923.8227149
GTAAGGT4900.023.1151984
TTACGCG650.002057598422.8755180-81
Produced by FastQC (version 0.11.5)